RESUMEN
In this study, a novel near-infrared fluorescent off-on probe for H2S based on seminaphthorhodafluor fluorophore is designed and constructed, which could be used in detection with 121-fold (23-fold) fluorescent (absorbance) enhancement at 630 nm (572 nm), fast responsiveness (completed within 5 min), high sensitivity, and lower cellular autofluorescence interference. Based on these excellent optical properties, the probe was employed to monitor H2S in red wine samples with satisfactory results. Moreover, the probe was successfully applied for monitoring and imaging H2S quantitatively in Hela cells and live athymic nude mice, indicating its potential application in biological science.
Asunto(s)
Benzopiranos/química , Colorantes Fluorescentes/química , Sulfuro de Hidrógeno/análisis , Naftoles/química , Imagen Óptica/métodos , Rodaminas/química , Animales , Células HeLa , Humanos , Rayos Infrarrojos , Ratones Desnudos , Microscopía Fluorescente/métodosRESUMEN
Herein, we presented two novel turn-on colorimetric and fluorescent probes based on a F- triggered SiO bond cleavage reaction, which displayed several desired properties for the quantitative detection for F-, such as high specificity, rapid response time (within 3â¯min) and naked-eye visualization. The fluorescence intensity at 574â¯nm (absorbance at 544â¯nm) of the solution was found to increase linearly with the concentration of F- (0.00-30.0⯵M) with the detection limit was estimated to be 0.47⯵M/0.48⯵M. Based on these excellent optical properties, the probes were employed to monitor F- in real water samples and tea samples with satisfactory. Furthermore, it was successfully applied for fluorescent imaging of F- in living nude mice, suggesting that it could be used as a powerful tool to predict and explore the biological functions of F- in physiological and pathological processes.