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Int J Syst Evol Microbiol ; 68(11): 3557-3562, 2018 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-30204586

RESUMEN

Two mycobacterial strains with close similarity to the Mycobacterium tuberculosis complex (MTBC) were isolated from cutaneous lesions of patients in the USA and Italy. At the phenotypic level, similarities to the MTBC included slow growth rate, rough morphotype of the unpigmented colonies and nearly identical high-performance liquid chromatography profiles of mycolic acids. In contrast to the MTBC, the strains were niacin- and nitrate-negative, and catalase-positive both at 68 °C and in semi-quantitative tests. The clinical isolates were more closely related to M. tuberculosis than to any other known mycobacterium and scored positive with commercial DNA probes (Hologic AccuProbe M. tuberculosis). Both average nucleotide identity and genome-to-genome distance suggested the strains are different from the MTBC. Therefore, given the distinguishing phenotypic and genomic-scale differences, we submit that the strains belong to a new species we have named Mycobacteriumdecipiens with type strain TBL 1200985T (=ATCC TSD-117T=DSM 105360T).


Asunto(s)
Infecciones por Mycobacterium/microbiología , Mycobacterium/clasificación , Filogenia , Tuberculosis Cutánea/microbiología , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Humanos , Italia , Mycobacterium/genética , Mycobacterium/aislamiento & purificación , Mycobacterium tuberculosis , Ácidos Micólicos/química , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Estados Unidos
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