1.
Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase.
Transgenic Res
; 23(1): 177-85, 2014 Feb.
Artículo
en Inglés
| MEDLINE
| ID: mdl-24197666
RESUMEN
We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This method results in rapid allele conversion and minimizes the use of experimental animals when compared to conventional Cre transgenic mouse breeding, resulting in a significant reduction in costs and time with increased welfare benefits.
Asunto(s)
Desarrollo Embrionario/genética , Integrasas/genética , Alelos , Animales , Cruzamiento , Embrión de Mamíferos , Células Madre Embrionarias , Ratones , Ratones Transgénicos
2.
Br J Hosp Med (Lond)
; 70(10): 599, 2009 Oct.
Artículo
en Inglés
| MEDLINE
| ID: mdl-20054910