Rapamycin alleviates irradiation-induced parotid injury by enhancing the whole gland homeostasis.

OBJECTIVES: Salivary gland injury is one of the most common complications of radiotherapy in head-and-neck cancers. This study investigated the mechanism by which rapamycin prevents irradiation (IR)-induced injury in the parotid glands. MATERIALS AND METHODS: Miniature pigs either received (a) no treatment (NT), (b) IR in the right parotid gland for 5 consecutive days (IR), or intraperitoneal administration of rapamycin (Rap) 1 h prior to IR (IR + Rap). Tissues were collected at three distinct time points (24 h, 4 weeks, and 16 weeks) after IR. Histological analyses, western blot, and real-time reverse transcriptase-polymerase chain reaction were performed to explore the mechanisms of IR-induced injury in the parotid gland. RESULTS: Rapamycin treatment maintained parotid salivary flow 16 weeks post-IR, preserved the number of acinar cells, and reduced parotid tissue fibrosis, as well as reduced apoptosis levels, decreased cleaved caspase-3 expression, and increased the Bcl-2/Bax ratio in the parotid glands. Autophagy marker LC3B was upregulated by rapamycin after IR, while P62 expression was downregulated. Rapamycin reduced the expression of pro-inflammatory factors and the mesenchymal tissue fibrosis following IR. CONCLUSIONS: Rapamycin maintains gland homeostasis after IR by decreasing apoptosis, reducing the expression of pro-inflammatory factors, and enhancing autophagy.

Selenium nanoparticles decorated with polysaccharides from Sargassum fusiforme protects against 6-OHDA-induced neurotoxicity in PC12 cells and rat model of Parkinson's disease.

Parkinson's disease (PD) is a neurodegenerative disorder and identifying disease-causing pathways and drugs that target them has remained challenging. Herein, selenium nanoparticles decorated with polysaccharides from Sargassum fusiforme (SFPS-SeNPs) were investigated on 6-OHDA-induced neurotoxicity in PC12 cells and rats. 6-OHDA can significantly increase neurotoxicity, oxidative stress and decrease the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) both in vitro and vivo. In vitro, treatment with SFPS-SeNPs can significantly decrease 6-OHDA cytotoxicity, reactive oxygen species (ROS) production or malondialdehyde (MDA) levels, and cell apoptosis, significantly increased the activity of SOD and GPx. In vivo, 6-OHDA exposure could also decrease the expression of Nrf2 and OH-1, while treatment with SFPS-SeNPs (1 mg Se/kg) increased. SFPS-SeNPs can protect neurons from 6-OHDA-induced neurotoxicity by regulating apoptosis and Nrf2/ARE pathway. The present study demonstrated that SFPS-SeNPs is a good candidate for developing a new drug against neurodegenerative diseases such as PD.

694 W sub-GHz polarization-maintained tapered fiber amplifier based on spectral and pump wavelength optimization.

The comprehensive suppression of the stimulated Brillouin scattering (SBS) and transverse mode instability (TMI) is a critical issue for the power scaling of fiber laser with sub-GHz spectral linewidth. In this manuscript, a narrow linewidth and polarization-maintained (PM) fiber amplifier based on tapered Yb-doped fiber (T-YDF) is established, and the effects of spectral linewidth, spectral shape and pump wavelength on the SBS and/or TMI thresholds are investigated. Up to 694 W polarization-maintained fiber laser with just ∼790 MHz linewidth is obtained by combining the advantages of tapered Yb-doped fiber, near-rectangular spectral injection and 915 nm pump manner. This work could provide a well reference solution for the realization of high-power ultra-narrow linewidth fiber lasers.

Experimental study on the impact of signal bandwidth on the transverse mode instability threshold of fiber amplifiers.

In this work, we conduct a detailed experimental study on the impact of signal bandwidth on the TMI threshold of fiber amplifiers. Both the filtered superfluorescent fiber sources and the phase-modulated single-frequency lasers are employed to construct seed lasers with different 3 dB spectral linewidths ranging from 0.19 nm to 7.97 nm. The TMI threshold of the fiber amplifier employing those seed lasers are estimated through the intensity evolution of the signal laser, and different criteria have been utilized to characterize the spectral linewidth of the seed lasers. Notably, the experimental results reveal that the TMI threshold of fiber amplifiers grows, keeps constant, and further grows as a function of spectral linewidth of seed lasers. Our experimental results could provide a well reference to understand the mechanism of the TMI effect and optimize the TMI effect in high-power fiber amplifiers.

Bidirectional tandem-pumped high-brightness 6†kW level narrow-linewidth confined-doped fiber amplifier exploiting the side-coupled technique.

In this work, a bidirectional tandem-pumped high-power narrow-linewidth confined-doped ytterbium fiber amplifier is demonstrated based on side-coupled combiners. Benefiting from the large-mode-area design of the confined-doped fiber, the nonlinear effects, including stimulated Raman (SRS) and stimulated Brillouin scattering (SBS), are effectively suppressed. While the transverse mode instability (TMI) effect is also mitigated through the combination of confined-doped fiber design and the bidirectional tandem pumping scheme. As a result, narrow-linewidth fiber laser with 5.96 kW output power is obtained, the slope efficiency and the 3-dB linewidth of which are ∼81.7% and 0.42 nm, respectively. The beam quality is well maintained during the power scaling process, being around M2 = 1.6 before the TMI occurs, and is well kept (M2 = 2.0 at 5.96 kW) even after the onset of TMI. No SRS or SBS is observed at the maximum output power, and the signal-to-noise ratio reaches as high as ∼61.4 dB. To the best of our knowledge, this is the record power ever reported in narrow-linewidth fiber lasers. This work could provide a good reference for realizing high-power high-brightness narrow-linewidth fiber lasers.

Identification of ocular refraction based on deep learning algorithm as a novel retinoscopy method.

BACKGROUND: The evaluation of refraction is indispensable in ophthalmic clinics, generally requiring a refractor or retinoscopy under cycloplegia. Retinal fundus photographs (RFPs) supply a wealth of information related to the human eye and might provide a promising approach that is more convenient and objective. Here, we aimed to develop and validate a fusion model-based deep learning system (FMDLS) to identify ocular refraction via RFPs and compare with the cycloplegic refraction. In this population-based comparative study, we retrospectively collected 11,973 RFPs from May 1, 2020 to November 20, 2021. The performance of the regression models for sphere and cylinder was evaluated using mean absolute error (MAE). The accuracy, sensitivity, specificity, area under the receiver operating characteristic curve, and F1-score were used to evaluate the classification model of the cylinder axis. RESULTS: Overall, 7873 RFPs were retained for analysis. For sphere and cylinder, the MAE values between the FMDLS and cycloplegic refraction were 0.50 D and 0.31 D, representing an increase of 29.41% and 26.67%, respectively, when compared with the single models. The correlation coefficients (r) were 0.949 and 0.807, respectively. For axis analysis, the accuracy, specificity, sensitivity, and area under the curve value of the classification model were 0.89, 0.941, 0.882, and 0.814, respectively, and the F1-score was 0.88. CONCLUSIONS: The FMDLS successfully identified the ocular refraction in sphere, cylinder, and axis, and showed good agreement with the cycloplegic refraction. The RFPs can provide not only comprehensive fundus information but also the refractive state of the eye, highlighting their potential clinical value.

3 kW power-level all-fiberized superfluorescent fiber source with linear polarization and near-diffraction-limited beam quality.

In this paper, we demonstrate a high-power linearly polarized all-fiberized superfluorescent fiber source (SFS) with near-diffraction-limited beam quality. The laser system is based on a master oscillator power amplifier configuration, which mainly consists of a polarization-maintained (PM) broadband SFS and multistage PM fiber amplifiers. A linearly polarized fiber laser over 3 kW is obtained at a pump power of about 4.3 kW with the corresponding slope efficiency of 71.9%. The polarization extinction ratio and the beam quality (M2 factor) of the output laser are about 12.7 dB and 1.18, respectively, at the maximum output power. To the best of our knowledge, this is the highest output power of a linearly polarized broadband SFS with near-diffraction-limited beam quality.

Dendritic Mesoporous Silica Nanoparticle Supported PtSn Catalysts for Propane Dehydrogenation.

PtSn catalysts were synthesized by incipient-wetness impregnation using a dendritic mesoporous silica nanoparticle support. The catalysts were characterized by XRD, N2 adsorption-desorption, TEM, XPS and Raman, and their catalytic performance for propane dehydrogenation was tested. The influences of Pt/Sn ratios were investigated. Changing the Pt/Sn ratios influences the interaction between Pt and Sn. The catalyst with a Pt/Sn ratio of 1:2 possesses the highest interaction between Pt and Sn. The best catalytic performance was obtained for the Pt1Sn2/DMSN catalyst with an initial propane conversion of 34.9%. The good catalytic performance of this catalyst is ascribed to the small nanoparticle size of PtSn and the favorable chemical state and dispersion degree of Pt and Sn species.

Comparisons of kilowatt Yb-Raman fiber amplifiers employing a superfluorescent fiber source and fiber oscillator.

In this paper, we demonstrate experimental investigations on kilowatt-level Yb-Raman fiber amplifiers (YRFAs) employing a superfluorescent fiber source (SFS) or a multi-longitudinal mode fiber oscillator (OSC) as the Raman-pump laser. Through comparing the output properties of the two YRFAs, the experimental results reveal that the YRFA employing the SFS is superior to the YRFA employing the OSC in the performances of power scalability and narrow-linewidth operation. Meanwhile, about 1.16 kW Raman-signal laser at 1120 nm is obtained through the YRFA employing the SFS as the Raman-pump laser. Overall, the presentation could provide an effective solution for the design of high-power narrow linewidth YRFAs.

High power tunable multiwavelength random fiber laser at 1.3 µm waveband.

Multiwavelength fiber lasers, especially those operating at optical communication wavebands such as 1.3 µm and 1.5 µm wavebands, have huge demands in wavelength division multiplexing communications. In the past decade, multiwavelength fiber lasers operating at 1.5 µm waveband have been widely reported. Nevertheless, 1.3 µm waveband multiwavelength fiber laser is rarely studied due to the lack of proper gain mechanism. Random fiber laser (RFL), owing to its good temporal stability and flexible wavelength tunability, is a great candidate for multiwavelength generation. Here, we reported high power multiwavelength generation at 1.3 µm waveband in RFL for the first time. At first, we employed a section of 10 km G655C fiber to provide Raman gains, as a result of which, 1.07 W multiwavelength generation at 1.3 µm waveband with an optical to signal noise ratio of ∼33 dB is demonstrated. By tuning the pump wavelength from 1055 nm to 1070 nm, tunable multiwavelength output covering the range of 1300-1330 nm can be achieved. Furtherly, we realized 4.67 W multiwavelength generation at 1.3 µm waveband by shortening the fiber length to 4 km. To the best of our knowledge, this is the highest output power ever reported for multiwavelength fiber lasers.

Temporally stable fiber amplifier pumped random distributed feedback Raman fiber laser with record output power.

In this Letter, we propose a scheme to use a temporally stable pump source in a high-power random distributed feedback Raman fiber laser (RRFL) with a half-open cavity. Different from conventional pump manners, the pump source is based on an Yb-doped fiber amplifier, seeded by a temporally stable phase-modulated single-frequency fiber laser for suppressing the spectral broadening and second-order Raman Stokes generation in the output laser. Using a piece of 50-m-long 20/400 µm passive fiber, the maximum output power of 1570 W was obtained with a pump power of 2025 W. The conversion efficiency with respect to the pump power was 77.5%. To the best of our knowledge, this is the highest output power ever reported in a RRFL to date. This work could provide a novel method for power scaling of RRFLs.

2 kW narrow-linewidth Yb-Raman fiber amplifier.

In this Letter, we report a high-power narrow-linewidth Yb-Raman fiber amplifier with a high second-order Raman threshold and high intensity stability. By employing two temporally stable seed lasers, over 2 kW output power at 1120 nm is achieved at a pump power of 2.6 kW with an optical-to-optical efficiency of 76.3%. The 3 dB linewidth of the 1120 nm Raman-signal laser varies slightly from 0.41 nm to 0.53 nm, and the power ratio of the second-order Raman Stokes light is only about ${-}{46.3}\;{\rm{dB}}$ at the output power of 2 kW. The results further confirm that the technique of employing temporally stable seed lasers is superior to the power scaling of narrow-linewidth Yb-Raman fiber amplifiers. To the best of our knowledge, it is the first demonstration of an over 2 kW narrow-linewidth fiber laser operating at 1120 nm.

Compact and low-cost superfluorescent fiber source assisted narrow linewidth Yb-Raman fiber amplifier.

Recent work has shown that temporally stable optical sources are required in a narrow linewidth Yb-Raman fiber amplifier to suppress the spectral broadening phenomenon. Superfluorescent fiber sources (SFSs) with different spectral widths are used as the Raman-pumped lasers in a 200-watt level narrow linewidth Yb-Raman fiber amplifier for the first time to the best of our knowledge. The experimental results reveal that the spectral broadening phenomenon could be well controlled by using the broadband SFS. Therefore, the narrow linewidth operation could be well maintained during the power scaling process. Moreover, the suppression of the spectral broadening phenomenon would deteriorate when the spectral width of the SFS decreases. This work could provide a compact, low-cost choice for the Raman-pumped laser in narrow linewidth Yb-Raman fiber amplifiers.

Dual-wavelength random distributed feedback fiber laser with wavelength, linewidth, and power ratio tunability.

Owing to the special power distribution property, a random distributed feedback Raman fiber laser can achieve a high power spectrally flexible output with a low power spectrally tuning device. Here, an all-fiberized linearly polarized dual-wavelength random distributed feedback Raman laser with wavelength, linewidth, and power ratio tunability is demonstrated. By adopting two watt-level bandwidth adjustable optical filters, a spectrum-manipulable dual-wavelength output with nearly a 10 W output power is achieved. The wavelength separation can be tuned from 2.5 to 13 nm, and the 3 dB linewidth of the output can be doubled by increasing the bandwidth of the optical filter. The power ratio of each laser line can be tuned from 0 to nearly 100% with the help of two variable optical attenuators. A maximum output power of 9.46 W is realized, with a polarization extinction ratio up to 20.5 dB. The proposed dual-wavelength fiber laser can be employed as a pump source in frequency tunable, bandwidth adjustable terahertz microwave generation, and mid-infrared optical parametric oscillators.

Power scaling of Raman fiber amplifier based on the optimization of temporal and spectral characteristics.

We comprehensively study the effects of temporal and spectral optimization on single-mode Raman fiber amplifiers. Amplified spontaneous emission sources and ytterbium-doped fiber lasers are employed as seed or pump lasers for comparison, and passive fibers are utilized as gain media. The influences of various parameters of the laser on 2nd order Raman threshold and maximum output power are investigated experimentally, including bandwidth, seed power, wavelength separation between pump and seed laser, and temporal stability. With the 190 m passive fiber, the output power increases from 99.5 W to 142.4 W, corresponding to 43.1% improvement through the optimization of seed laser power, pump wavelength and temporal performance of pump source in this amplifier, which has guidance on the establishment of high-power single-mode Raman fiber amplifiers.

Cascaded telecom fiber enabled high-order random fiber laser beyond zero-dispersion wavelength.

Four-wave mixing induced spectral broadening near the zero-dispersion wavelength (ZDW) of the fiber is a bottleneck factor that limits the further wavelength extending in cascaded random fiber lasers (RFLs). In this Letter, we successfully suppress the spectral broadening near the ZDW of the fiber in the cascaded RFL by simply combining two kinds of commercial telecom fibers with different ZDWs, G655C fiber with ZDW around 1.52 µm and G652D fiber with ZDW around 1.31 µm. As a result, an 8th order Stokes light component at 1721 nm with a maximum output power of 2.1 W and a spectral purity of 96.94% is realized in this telecom-fiber-based cascaded RFL. This work provides a reference of nonlinear effect management in fiber lasers as well as affords a cost-effective way with great potential of realizing high-power widely tunable fiber lasers.

Broadband pumping enabled flat-amplitude multi-wavelength random Raman fiber laser.

A flat-amplitude multi-wavelength random Raman fiber laser with broad spectral coverage and a high optical signal-to-noise ratio (OSNR) is challenging and of great interest. In this Letter, we theoretically and experimentally proved that broadband pumping can help realize a broader, flat-amplitude multi-wavelength random Raman fiber laser. The influence of pump bandwidth, tunability of the spectral envelope, and channel spacing are investigated. As a result, with a 40 nm pump bandwidth, a spectral coverage of 1116-1125 nm with 19 laser lines and 31 dB OSNR is achieved, and the standard deviation in the peak intensities of the central nine lines is ${\sim}{1}.{1}\;{\rm dBm}$∼1.1dBm. This technique can also be applied to the multi-wavelength Raman (or random Raman) fiber lasers at other wavelengths and provide a reference for multi-wavelength applications in sensing, communication, and optical component testing.

Sphingosine-1-phosphate alleviates irradiation-induced parotid injury in a miniature pig model.

OBJECTIVES: Our aim was to verify the alleviation effect of sphingosine-1-phosphate (S1P) in a miniature pig model. MATERIAL AND METHODS: Thirty male miniature pigs were randomly separated into 10 groups in our experiment. We administered S1P through the parotid duct in a retrograde fashion 2 hr before irradiation (IR). The salivary flow rate and blood flow rate were tested 20 weeks after IR. The apoptotic level was checked at 12, 24 hr and 7 days post-IR. RESULTS: Twenty weeks after IR, the salivary flow rate of the IR-side parotid gland in IR + S1P group can be maintained at about 40% of the non-IR side, while only 20% was maintained in the IR group. The blood flow rate and microvascular density were significantly higher in the IR + S1P group than in the IR group. The apoptotic level and cleaved caspase-3 expression were downregulated in IR + S1P group, and the ratio of Bcl-2/Bax was increased. The blood flow rate and CD31 level were significantly restored at 12, 24 hr and 7 days post-IR. CONCLUSION: Sphingosine-1-phosphate may partially alleviate IR-induced parotid dysfunction by decreasing apoptosis of microvascular endothelial cells and maintaining the blood flow rate.

Tunable random Raman fiber laser at 1.7 µm region with high spectral purity.

We demonstrate a tunable, high order cascaded random Raman fiber laser (RRFL) with high purity at 1.7 µm band by using a high power amplified spontaneous emission source (ASE) with both wavelength and linewidth tunability as pump source. The influence of the spectral bandwidth of the ASE source on the spectral purity of the output at 1.7 µm band is investigated. By adjusting the spectral bandwidth of the ASE source to the optimized 20 nm, output power >14 W with spectral purity up to 98.29% at 1715 nm is achieved. As far as we know, this is the highest spectral purity ever reported for a RRFL at 1.7 µm region. Furthermore, by adjusting the central wavelength of ASE source, the output of the RRFL can be tuned from 1695 to 1725 nm with >10 W output power. What's more, the spectral purity is above 92% over a tuning range from 1705 to 1725 nm.

Phosphosilicate fiber-based dual-wavelength random fiber laser with flexible power proportion and high spectral purity.

Phosphosilicate fiber has the inherent advantage of generating dual-wavelength output owing to the two Raman gain peaks at the frequency shifts of ∼13.2 THz (silica-related) and 39.9 THz (phosphorus-related), respectively. The frequency shift of 39.9 THz is often adopted to obtain long wavelength laser, while the control of Stokes light at 13.2 THz has attracted much attention currently. In this paper, a dual-wavelength random distributed feedback Raman fiber laser (RDFL) with over 100 nm wavelength interval and continuously tunable power proportion was presented based on phosphosilicate fiber for the first time. Through using the filtered amplified spontaneous emission (ASE) source as the pump source, the spectral purity of the Stokes light could be as high as 99.8%. By tuning two manual variable optical attenuators (VOAs), the power proportion of the silica-related Stokes light could range from ∼0% to 99.0%, and the maximum value is limited by the generation of second order Stokes light. Although the power handling capability of the VOA is merely 2 W, over 23 W total output power of the Stokes light was obtained thanks to the particular power distribution property of RDFL. This experiment demonstrates the potential to achieve a flexible high-power and high-spectral purity dual-wavelength RDFL output.