RESUMEN
SUMMARY: Understanding developmental processes and building towards integrative systems biology require detailed knowledge of the spatio-temporal expression of genes and proteins. We have developed a software package for collecting, storing and searching the annotation of protein or gene expression patterns in Drosophila melanogaster. Using standard Drosophila anatomy and Gene Ontologies, the system can readily capture expression patterns at any stage of development and in all recognized tissue types as well as details of sub-cellular localization. The web-based system allows multiple groups to work in collaboration and share images and annotation. AVAILABILITY: http://www.flannotator.org.uk/.
Asunto(s)
Biología Computacional/métodos , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/genética , Programas Informáticos , Animales , Bases de Datos Genéticas , Bases de Datos de Proteínas , Proteínas de Drosophila/genética , Drosophila melanogaster/metabolismo , Genes de Insecto , Internet , Interfaz Usuario-ComputadorAsunto(s)
Encefalopatías/genética , Dermatoglifia , Síndrome de Down/genética , Epilepsia , Femenino , Humanos , Hidrocefalia , Masculino , Microcefalia , FenilcetonuriasRESUMEN
We present here the first fully integrated, comprehensive map of the canine genome, incorporating detailed cytogenetic, radiation hybrid (RH), and meiotic information. We have mapped a collection of 266 chromosome-specific cosmid clones, each containing a microsatellite marker, to all 38 canine autosomes by fluorescence in situ hybridization (FISH). A 1500-marker RH map, comprising 1078 microsatellites, 320 dog gene markers, and 102 chromosome-specific markers, has been constructed using the RHDF5000-2 whole-genome radiation hybrid panel. Meiotic linkage analysis was performed, with at least one microsatellite marker from each dog autosome on a panel of reference families, allowing one meiotic linkage group to be anchored to all 38 dog autosomes. We present a karyotype in which each chromosome is identified by one meiotic linkage group and one or more RH groups. This updated integrated map, containing a total of 1800 markers, covers >90% of the dog genome. Positional selection of anchor clones enabled us, for the first time, to orientate nearly all of the integrated groups on each chromosome and to evaluate the extent of individual chromosome coverage in the integrated genome map. Finally, the inclusion of 320 dog genes into this integrated map enhances existing comparative mapping data between human and dog, and the 1000 mapped microsatellite markers constitute an invaluable tool with which to perform genome scanning studies on pedigrees of interest.