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High-resolution neutron and THz spectroscopies are used to study the magnetic excitation spectrum of Cs_{2}CoBr_{4}, a distorted-triangular-lattice antiferromagnet with nearly XY-type anisotropy. What was previously thought of as a broad excitation continuum [L. Facheris et al., Phys. Rev. Lett. 129, 087201 (2022)PRLTAO0031-900710.1103/PhysRevLett.129.087201] is shown to be a series of dispersive bound states reminiscent of "Zeeman ladders" in quasi-one-dimensional Ising systems. At wave vectors where interchain interactions cancel at the mean field level, they can indeed be interpreted as bound finite-width kinks in individual chains. Elsewhere in the Brillouin zone their true two-dimensional structure and propagation are revealed.
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Memoria , Neutrones , Anisotropía , ReproducciónRESUMEN
Acanthocephalans are parasites with complex lifecycles that are important components of aquatic systems and are often model species for parasite-mediated host manipulation. Genetic characterization has recently resurrected Pomphorhynchus tereticollis as a distinct species from Pomphorhynchus laevis, with potential implications for fisheries management and host manipulation research. Morphological and molecular examinations of parasites from 7 English rivers across 9 fish species revealed that P. tereticollis was the only Pomphorhynchus parasite present in Britain, rather than P. laevis as previously recorded. Molecular analyses included two non-overlapping regions of the mitochondrial gene - cytochrome oxidase and generated 62 sequences for the shorter fragment (295 bp) and 74 for the larger fragment (583 bp). These were combined with 61 and 13 sequences respectively, from Genbank. A phylogenetic analysis using the two genetic regions and all the DNA sequences available for P. tereticollis identified two distinct genetic lineages in Britain. One lineage, possibly associated with cold water tolerant fish, potentially spread to the northern parts of Britain from the Baltic region via a northern route across the estuarine area of what is now the North Sea during the last Glaciation. The other lineage, associated with temperate freshwater fish, may have arrived later via the Rhine/Thames fluvial connection during the last glaciation or early Holocene when sea levels were low. These results raise important questions on this generalist parasite and its variously environmentally adapted hosts, and especially in relation to the consequences for parasite vicariance.
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Acantocéfalos/fisiología , Distribución Animal , Clima , Peces/parasitología , Interacciones Huésped-Parásitos , Tolerancia a la Sal , Acantocéfalos/genética , Acantocéfalos/crecimiento & desarrollo , Animales , Inglaterra , Proteínas del Helminto/análisis , Larva/genética , Larva/crecimiento & desarrollo , Larva/fisiología , Filogenia , RíosAsunto(s)
COVID-19 , Dermatología , Telemedicina , Hospitales , Humanos , Pandemias , Derivación y ConsultaRESUMEN
Normal-to-cancer (NTC) transition is known to be closely associated to cell´s biomechanical properties which are dependent on the dynamics of the intracellular medium. This study probes different human cancer cells (breast, prostate and lung), concomitantly to their healthy counterparts, aiming at characterising the dynamical profile of water in distinct cellular locations, for each type of cell, and how it changes between normal and cancer states. An increased plasticity of the cytomatrix is observed upon normal-to-malignant transformation, the lung carcinoma cells displaying the highest flexibility followed by prostate and breast cancers. Also, lung cells show a distinct behaviour relative to breast and prostate, with a higher influence from hydration water motions and localised fast rotations upon NTC transformation. Quasielastic neutron scattering techniques allowed to accurately distinguish the different dynamical processes taking place within these highly heterogeneous cellular systems. The results thus obtained suggest that intracellular water dynamics may be regarded as a specific reporter of the cellular conditions-either healthy or malignant.
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Neoplasias , Agua , Humanos , Difracción de Neutrones , NeutronesRESUMEN
AIM: A new procedure was developed to recover adenovirus 41 in small volumes (1 l) of water samples based on adsorption, elution and evaporation. METHODS AND RESULTS: One litre of source water seeded with adenovirus 41 was adjusted to pH 3·5 and filtered using a large pore size (8·0 µm) negatively charged membrane filter (SCWP, 47 mm diameter, made of mixed-cellulose esters). Then, the filter was eluted using 4 ml of 1·5% beef extract plus 0·75% glycerol (pH 9·0). The eluate was reconcentrated to 0·1 ml or less volumes through evaporation assisted with air flow and heating at 55°C. Recovery of adenovirus 41 reached 55% under tested conditions and reduced filtration time by 85% in contrast to the widely used small pore size filter (0·45 µm pore size, 47 mm diameter). Reconcentration by evaporation achieved approx. 86·8% recovery from source water in approx. 1 h at no cost. CONCLUSION: The virus concentration method developed in this study is simple and cost-effective and can be used to efficiently recover adenovirus 41 from turbid water samples. SIGNIFICANCE AND IMPACT OF THE STUDY: The procedure developed can be applied to detect adenovirus 41 in source water within hours of sampling. In addition, this is the first application of evaporation to concentrate viruses in water samples.
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Adenoviridae/aislamiento & purificación , Monitoreo del Ambiente/métodos , Técnicas Microbiológicas/métodos , Microbiología del Agua , Agua/análisis , Adsorción , Monitoreo del Ambiente/instrumentación , Filtración/instrumentación , Técnicas Microbiológicas/instrumentación , Contaminación del Agua/análisis , Abastecimiento de Agua/análisisRESUMEN
AIMS: Research into the relationship between pathogens, faecal indicator microbes and environmental factors in beach sand has been limited, yet vital to the understanding of the microbial relationship between sand and the water column and to the improvement of criteria for better human health protection at beaches. The objectives of this study were to evaluate the presence and distribution of pathogens in various zones of beach sand (subtidal, intertidal and supratidal) and to assess their relationship with environmental parameters and indicator microbes at a non-point source subtropical marine beach. METHODS AND RESULTS: In this exploratory study in subtropical Miami (Florida, USA), beach sand samples were collected and analysed over the course of 6 days for several pathogens, microbial source tracking markers and indicator microbes. An inverse correlation between moisture content and most indicator microbes was found. Significant associations were identified between some indicator microbes and pathogens (such as nematode larvae and yeasts in the genus Candida), which are from classes of microbes that are rarely evaluated in the context of recreational beach use. CONCLUSIONS: Results indicate that indicator microbes may predict the presence of some of the pathogens, in particular helminthes, yeasts and the bacterial pathogen Staphylococcus aureus including methicillin-resistant forms. Indicator microbes may thus be useful for monitoring beach sand and water quality at non-point source beaches. SIGNIFICANCE AND IMPACT OF THE STUDY: The presence of both indicator microbes and pathogens in beach sand provides one possible explanation for human health effects reported at non-point sources beaches.
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Bacterias/aislamiento & purificación , Playas , Monitoreo del Ambiente/métodos , Helmintos/aislamiento & purificación , Microbiología del Agua , Levaduras/aislamiento & purificación , Animales , Recuento de Colonia Microbiana , Florida , Agua de Mar/microbiología , Agua de Mar/parasitología , Dióxido de Silicio/análisisRESUMEN
AIMS: To develop a quantitative, real-time PCR assay to detect the nifH gene of Methanobrevibacter smithii. Methanobrevibacter smithii is a methanogenic archaea found in the intestinal tract of humans that may be a useful indicator of sewage pollution in water. METHODS AND RESULTS: Quantification standards were prepared from Meth. smithii genomic DNA dilutions, and a standard curve was used to quantify the target gene and calculate estimated genome equivalency units. A competitive internal positive control was designed and incorporated into the assay to assess inhibition in environmental extracts. Testing the assay against a panel of 23 closely related methanogen species demonstrated specificity of the assay for Meth. smithii. A set of 36 blind water samples was then used as a field test of the assay. The internal control identified varying levels of inhibition in 29 of 36 (81%) samples, and the Meth. smithii target was detected in all water samples with known sewage input. CONCLUSIONS: The quantitative PCR assay developed in this study is a sensitive and rapid method for the detection of the Meth. smithii nifH gene that includes an internal control to assess inhibition. Further research is required both to better evaluate host specificity of this assay and the correlation with human health risks. SIGNIFICANCE AND IMPACT OF THE STUDY: This research is the first description of the development of a rapid and sensitive quantitative assay for a methanogenic archaeal indicator of sewage pollution.
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Monitoreo del Ambiente/métodos , Methanobrevibacter/genética , Oxidorreductasas/genética , Reacción en Cadena de la Polimerasa/métodos , Aguas del Alcantarillado/microbiología , Contaminantes del Agua/aislamiento & purificación , Cartilla de ADN/genética , ADN Bacteriano/aislamiento & purificación , Genes Bacterianos , Methanobrevibacter/aislamiento & purificación , Sensibilidad y Especificidad , Especificidad de la Especie , Microbiología del AguaRESUMEN
Neutron spectroscopy and diffuse neutron scattering on herbertsmithite [ZnCu(3)(OH)(6)Cl(2)], a near-ideal realization of the s=1/2 kagome antiferromagnet, reveal the hallmark property of a quantum spin liquid: instantaneous short-ranged antiferromagnetic correlations in the absence of a time-averaged ordered moment. These dynamic antiferromagnetic correlations are weakly dependent of neutron-energy transfer and temperature, and persist up to 25 meV and 120 K. At low energy transfers a shift of the magnetic scattering to low Q is observed with increasing temperature, providing evidence of gapless spinons. It is argued that these observations provide important evidence in favor of resonating-valence-bond theories of (doped) Mott insulators.
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The Quaternary fossil record has abundant evidence for ecologically nonanalogue communities made up of combinations of modern taxa not seen in sympatry today. A brief review of the literature detailing these nonanalogue communities is given with a discussion of their various proposed causes. The individualistic, Gleasonian, response of species to climate and environmental change is favoured by many. The degree to which communities are nonanalogue appears to increase with greater time depth, and this progressive process is a necessary outcome of the individualistic response of species to climate change through time. In addition, it is noted that populations within species, as well as the species as a whole, respond individualistically. This paper proposes that many elements of nonanalogue communities are extinct populations, which may explain their environmentally anomalous combinations. These extinct populations are, by definition, lineages without descendents. It is further proposed that the differential extinction of populations, as a result of continuous ecological reassembly, could amount to a significant evolutionary phenomenon.
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Evolución Biológica , Cambio Climático , Animales , Ecosistema , FósilesRESUMEN
Cells of Acanthamoeba castellanii (Neff) are known to form mature cysts characterized by a cellulose-containing cell wall when transferred to a nonnutrient medium. Amebas which engulfed latex beads before encystment formed mature cysts essentially devoid of bead material. The encystment of bead-containing cells appeared to be similar to that of control cells since no important differences between the two were observed with respect to cellular levels of glycogen or protein, cellulose synthetase activity, the amount of cyst wall polysaccharide formed, or the percentage of cysts formed. Actinomycin D and cycloheximide inhibited encystment as well as bead expulsion. Ultrastructural analysis revealed that the beads, which initially were contained in phagocytic vesicles, were released from the cell by fusion of vesicular membranes with the plasma membrane. Exocytosis was observed in cells after 3 hr of encystment, with most of the beads being lost before cyst wall formation. Each bead-containing vesicle involved in expulsion was conspicuously demarcated by an area of concentrated cytoplasm, which was more homogeneously granular than the surrounding cytoplasm. Beads were not observed in the cytoplasm of mature cysts but were occasionally found in the cyst wall.
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Eucariontes/citología , Látex , Microesferas , Animales , Membrana Celular/fisiología , Núcleo Celular , Pared Celular/análisis , Celulosa/análisis , Medios de Cultivo , Cicloheximida/farmacología , Citoplasma/análisis , Dactinomicina/farmacología , Eucariontes/análisis , Eucariontes/efectos de los fármacos , Eucariontes/enzimología , Eucariontes/fisiología , Glucógeno/análisis , Cuerpos de Inclusión/fisiología , Ligasas/análisis , Metamorfosis Biológica/efectos de los fármacos , Métodos , Microscopía Electrónica , Microtúbulos , Fagocitosis , Polisacáridos/análisis , Proteínas/análisis , Factores de TiempoRESUMEN
A myxobacterium (Cytophaga N-5) isolated from sewage kills or lyses an array of living green and blue-green algae. When assayed with Nostoc muscorum or Plectonema boryanum, plaques form like those caused by the blue-green algal virus LPP-1. This isolate lyses or inhibits mutually Gram-positive and Gram-negative eubacteria.
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Cytophaga/aislamiento & purificación , Eucariontes , Técnicas Bacteriológicas , Medios de Cultivo , Cytophaga/clasificación , Cytophaga/crecimiento & desarrollo , Cytophaga/patogenicidad , Microscopía Electrónica , Aguas del AlcantarilladoRESUMEN
We present a study of the magnetic ground state properties of ß-Mn metal alloyed with Co, using neutron polarization analysis of the diffuse neutron scattering cross-section. We analyse the magnetic structure obtained using a reverse Monte Carlo procedure to extract the Mn-Mn spin correlations. The addition of Co leads to a static disordered magnetic structure with medium-range correlations. Our analysis of the spin correlations indicates that both 8c and 12d non-equivalent lattice sites present in the ß-Mn structure contribute to the magnetic ground state, where previously it was thought that the 8c site was non-magnetic.
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The MAPS direct geometry time-of-flight chopper spectrometer at the ISIS pulsed neutron and muon source has been in operation since 1999, and its novel use of a large array of position-sensitive neutron detectors paved the way for a later generations of chopper spectrometers around the world. Almost two decades of experience of user operations on MAPS, together with lessons learned from the operation of new generation instruments, led to a decision to perform three parallel upgrades to the instrument. These were to replace the primary beamline collimation with supermirror neutron guides, to install a disk chopper, and to modify the geometry of the poisoning in the water moderator viewed by MAPS. Together, these upgrades were expected to increase the neutron flux substantially, to allow more flexible use of repetition rate multiplication and to reduce some sources of background. Here, we report the details of these upgrades and compare the performance of the instrument before and after their installation as well as to Monte Carlo simulations. These illustrate that the instrument is performing in line with, and in some respects in excess of, expectations. It is anticipated that the improvement in performance will have a significant impact on the capabilities of the instrument. A few examples of scientific commissioning are presented to illustrate some of the possibilities.
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A new paradigm of simulating suspended sediment load (SSL) with a Land Surface Model (LSM) is presented here. Five erosion and SSL algorithms were applied within a common LSM framework to quantify uncertainties and evaluate predictability in two steep, forested catchments (>1,000 km2). The algorithms were chosen from among widely used sediment models, including empirically based: monovariate rating curve (MRC) and the Modified Universal Soil Loss Equation (MUSLE); stochastically based: the Load Estimator (LOADEST); conceptually based: the Hydrologic Simulation Program-Fortran (HSPF); and physically based: the Distributed Hydrology Soil Vegetation Model (DHSVM). The algorithms were driven by the hydrologic fluxes and meteorological inputs generated from the Variable Infiltration Capacity (VIC) LSM. A multiobjective calibration was applied to each algorithm and optimized parameter sets were validated over an excluded period, as well as in a transfer experiment to a nearby catchment to explore parameter robustness. Algorithm performance showed consistent decreases when parameter sets were applied to periods with greatly differing SSL variability relative to the calibration period. Of interest was a joint calibration of all sediment algorithm and streamflow parameters simultaneously, from which trade-offs between streamflow performance and partitioning of runoff and base flow to optimize SSL timing were noted, decreasing the flexibility and robustness of the streamflow to adapt to different time periods. Parameter transferability to another catchment was most successful in more process-oriented algorithms, the HSPF and the DHSVM. This first-of-its-kind multialgorithm sediment scheme offers a unique capability to portray acute episodic loading while quantifying trade-offs and uncertainties across a range of algorithm structures.
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Incidences of harmful algal blooms (HABs) and Vibrio infections have increased over recent decades. Numerous studies have tried to identify environmental factors driving HABs and pathogenic Vibrio populations separately. Few have considered the two simultaneously, though emerging evidence suggests that algal blooms enhance Vibrio growth and survival. This study examined various physical, nutrient, and temporal factors associated with incidences of HABs, V. vulnificus, and V. parahaemolyticus in South Carolina coastal stormwater detention ponds, managed systems where HABs often proliferate, and their receiving tidal creek waters. Five blooms occurred during the study (2008-2009): two during relatively warmer months (an August 2008 cyanobacteria bloom and a November 2008 dinoflagellate bloom) followed by increases in both Vibrio species and V. parahaemolyticus, respectively, and three during cooler months (December 2008 through February 2009) caused by dinoflagellates and euglenophytes that were not associated with marked changes in Vibrio abundances. Vibrio concentrations were positively and significantly associated with temperature and dissolved organic matter, dinoflagellate blooms, negatively and significantly associated with suspended solids, but not significantly correlated with chlorophyll or nitrogen. While more research involving longer time series is needed to increase robustness, findings herein suggest that certain HAB species may augment Vibrio occurrences during warmer months.
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Portions of two commercial citrus orchards were treated for two consecutive years with buprofezin or three consecutive years with pyriproxyfen in a replicated plot design to determine the long-term impact of these insect growth regulators (IGRs) on the San Joaquin Valley California integrated pest management program. Pyriproxyfen reduced the target pest, California red scale, Aonidiella aurantii Maskell, to nondetectable levels on leaf samples approximately 4 mo after treatment. Pyriproxyfen treatments reduced the California red scale parasitoid Aphytis melinus DeBach to a greater extent than the parasitoid Comperiella bifasciata Howard collected on sticky cards. Treatments of lemons Citrus limon (L.) Burm. f. infested with scale parasitized by A. melinus showed only 33% direct mortality of the parasitoid, suggesting the population reduction observed on sticky cards was due to low host density. Three years of pyriproxyfen treatments did not maintain citricola scale, Coccus pseudomagnoliarum (Kuwana), below the treatment threshold and cottony cushion scale, Icerya purchasi Maskell, was slowly but incompletely controlled. Buprofezin reduced California red scale to very low but detectable levels approximately 5 mo after treatment. Buprofezin treatments resulted in similar levels of reduction of the two parasitoids A. melinus and C. bifasciata collected on sticky cards. Treatments of lemons infested with scale parasitized by A. melinus showed only 7% mortality of the parasitoids, suggesting the population reduction observed on sticky cards was due to low host density. Citricola scale was not present in this orchard, and cottony cushion scale was slowly and incompletely controlled by buprofezin. These field plots demonstrated that IGRs can act as organophosphate insecticide replacements for California red scale control; however, their narrower spectrum of activity and disruption of coccinellid beetles can allow other scale species to attain primary pest status.
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Citrus sinensis/parasitología , Hemípteros , Hormonas Juveniles , Piridinas , Tiadiazinas , Animales , California , Ecosistema , Interacciones Huésped-Parásitos , Control de Insectos/métodos , Factores de Tiempo , Avispas/fisiologíaRESUMEN
The biological rationale for hyperthermia and radiation is strong, initial results in superficial tumor sites have established efficacy in human cancer, and deep tumor sites are beginning to yield to our efforts at deep regional heating. Major thrusts of new clinical research should go toward improved equipment and defining techniques with existing devices, which can lead us into definitive Phase III trials to assess the role of regional hyperthermia in primary multimodality management of cancer. Detailed thermometry is an absolute requirement for all future clinical studies. Invasive techniques to achieve deep hyperthermia are under investigation and may prove to be useful. Whole-body hyperthermia with radiation and/or chemotherapy has been less well studied in the laboratory than has local hyperthermia, and studies to show clinical efficacy in humans are lacking.
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Hipertermia Inducida , Neoplasias/radioterapia , Neoplasias/terapia , Ensayos Clínicos como Asunto , Terapia Combinada , Evaluación de Medicamentos , Fenómenos Electromagnéticos , Humanos , Terapia por UltrasonidoRESUMEN
Industrial hog operations (IHOs) have been identified as a source of antibiotic-resistant Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA). However, few studies have investigated the presence of antibiotic-resistant S. aureus in the environment near IHOs, specifically surface waters proximal to spray fields where IHO liquid lagoon waste is sprayed. Surface water samples (n=179) were collected over the course of approximately one year from nine locations in southeastern North Carolina and analyzed for the presence of presumptive MRSA using CHROMagar MRSA media. Culture-based, biochemical, and molecular tests, as well as matrix-assisted laser desorption/ionization-time of flight mass spectrometry were used to confirm that isolates that grew on CHROMagar MRSA media were S. aureus. Confirmed S. aureus isolates were then tested for susceptibility to 16 antibiotics and screened for molecular markers of MRSA (mecA, mecC) and livestock adaptation (absence of scn). A total of 12 confirmed MRSA were detected in 9 distinct water samples. Nine of 12 MRSA isolates were also multidrug-resistant (MDRSA [i.e., resistant to ≥3 antibiotic classes]). All MRSA were scn-positive and most (11/12) belonged to a staphylococcal protein A (spa) type t008, which is commonly associated with humans. Additionally, 12 confirmed S. aureus that were methicillin-susceptible (MSSA) were recovered, 7 of which belonged to spa type t021 and were scn-negative (a marker of livestock-adaptation). This study demonstrated the presence of MSSA, MRSA, and MDRSA in surface waters adjacent to IHO lagoon waste spray fields in southeastern North Carolina. To our knowledge, this is the first report of waterborne S. aureus from surface waters proximal to IHOs.
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Antibacterianos/farmacología , Resistencia a la Meticilina , Staphylococcus aureus Resistente a Meticilina/aislamiento & purificación , Meticilina/farmacología , Ríos/microbiología , Crianza de Animales Domésticos/métodos , Animales , Staphylococcus aureus Resistente a Meticilina/genética , Pruebas de Sensibilidad Microbiana , North Carolina , Fenotipo , Sus scrofaRESUMEN
Twenty-eight patients with advanced upper-abdominal malignancy were treated at the University of Utah on a pilot protocol involving regional hyperthermia (HT) produced by the BSD-1000 HT system and the annular phased array applicator (AA), usually driven at 60 MHz. Eighty-two percent of the patients had widespread metastatic disease, and the mean tumor burden was 2,900 cc. Seventy-nine percent of the patients received concurrent radiotherapy. Acute toxicity consisted primarily of pain within the AA aperture (43%), systemic stress (43%), and nausea or vomiting (29%). Systemic stress was the most frequent power-limiting factor (46%). There were two treatment-related complications: sciatic neuritis from intramuscular injection (one) and pleural effusion from thermometry probe placement (one). Detailed thermal mapping and thermal dosimetry were performed on 26 patients. The mean thermal dosimetry parameters were quite low. Concurrent radiation doses were also quite low (mean, 1,500 rad) to avoid toxicity of sensitive organ systems within the abdomen. The objective response rate was only 18%, all partial, but 43% of the patients achieved effective symptomatic palliation. The five objective responders did survive significantly (P = .02) longer than the 23 nonresponders.
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Neoplasias Abdominales/terapia , Hipertermia Inducida/instrumentación , Terapia Combinada , Estudios de Evaluación como Asunto , Femenino , Humanos , Hipertermia Inducida/efectos adversos , Masculino , Persona de Mediana Edad , Metástasis de la Neoplasia , Aceleradores de Partículas , Dosificación Radioterapéutica , Termómetros , Factores de TiempoRESUMEN
The endomyocardial residual effects of left ventricular endocardial electrical ablation utilizing unipolar and bipolar electrode catheters were studied in 15 dogs. Histopathologic techniques specific for contraction band necrosis revealed that the mean maximal depth and breadth of necrosis was 0.63 +/- 0.44 and 1.23 +/- 0.82 cm, respectively. The dimensions of necrosis were significantly increased when utilizing larger energy discharges, especially through unipolar electrodes. Four dogs died during the procedure, three from ventricular fibrillation and one from asystole, and two died suddenly within the succeeding 24 hours. Endocardial thrombi were noted at necropsy in two dogs. In conclusion, transcatheter endocardial electrical ablation may destroy a sufficient mass of myocardium to interrupt arrhythmogenic conduction tissue, especially when larger currents are delivered through unipolar electrodes. However, serious ventricular arrhythmias and endocardial thrombi should be anticipated.