Calsyntenins mediate TGN exit of APP in a kinesin-1-dependent manner.

Kinesin motors are required for the export of membranous cargo from the trans-Golgi network (TGN), yet information about how kinesins are recruited to forming transport intermediates is sparse. Here we show that the Kinesin-1 docking protein calsyntenin-1 localizes to the TGN in vivo and directly and specifically recruits Kinesin-1 to Golgi/TGN membranes as well as to dynamic post-Golgi carriers. Overexpression of various calsyntenin chimeras and kinesin light chain 1 (KLC1) at high levels caused the formation of aberrant membrane stacks at the endoplasmic reticulum (ER) or the Golgi, disrupted overall Golgi structure and blocked exit of calsyntenin from the TGN. Intriguingly, this blockade of calsyntenin exit strongly and selectively impeded TGN exit of amyloid precursor protein (APP). Using live cell microscopy we found that calsyntenins exit the TGN in Kinesin-1-decorated tubular structures which may serve as carriers for calsyntenin-1-mediated post-TGN transport of APP. Abrogation of this pathway via virus-mediated knockdown of calsyntenin-1 expression in primary cultured neurons caused a marked elevation of APP C-terminal fragments. Together, these results indicate a role for calsyntenin-1 in Kinesin-1-dependent TGN exit and post-Golgi transport of APP-containing organelles and further suggest that distinct intracellular routes may exhibit different capacities for proteolytic processing of APP.

Molecular characterization of a trafficking organelle: dissecting the axonal paths of calsyntenin-1 transport vesicles.

Kinesin motors play crucial roles in the delivery of membranous cargo to its destination and thus for the establishment and maintenance of cellular polarization. Recently, calsyntenin-1 was identified as a cargo-docking protein for Kinesin-1-mediated axonal transport of tubulovesicular organelles along axons of central nervous system neurons. To further define the function of calsyntenin-1, we immunoisolated calsyntenin-1 organelles from murine brain homogenates and determined their proteome by MS. We found that calsyntenin-1 organelles are endowed with components of the endosomal trafficking machinery and contained the ß-amyloid precursor protein (APP). Detailed biochemical analyses of calsyntenin-1 immunoisolates in conjunction with immunocytochemical colocalization studies with cultured hippocampal neurons, using endosomal marker proteins for distinct subcompartments of the endosomal pathways, indicated that neuronal axons contain at least two distinct, nonoverlapping calsyntenin-1-containing transport packages: one characterized as early-endosomal, APP positive, the other as recycling-endosomal, APP negative. We postulate that calsyntenin-1 acts as a general mediator of anterograde axonal transportation of endosomal vesicles. In this role, calsyntenin-1 may actively contribute to axonal growth and pathfinding in the developing as well as to the maintenance of neuronal polarity in the adult nervous system; further, it may actively contribute to the stabilization of APP during its anterograde axonal trajectory.

Neurotrypsin cleaves agrin locally at the synapse.

The synaptic serine protease neurotrypsin is considered to be essential for the establishment and maintenance of cognitive brain functions, because humans lacking functional neurotrypsin suffer from severe mental retardation. Neurotrypsin cleaves agrin at two homologous sites, liberating a 90-kDa and a C-terminal 22-kDa fragment from the N-terminal moiety of agrin. Morphological analyses indicate that neurotrypsin is contained in presynaptic terminals and externalized in association with synaptic activity, while agrin is localized to the extracellular space at or in the vicinity of the synapse. Here, we present a detailed biochemical analysis of neurotrypsin-mediated agrin cleavage in the murine brain. In brain homogenates, we found that neurotrypsin exclusively cleaves glycanated variants of agrin. Studies with isolated synaptosomes obtained by subcellular fractionation from brains of wild-type and neurotrypsin-overexpressing mice revealed that neurotrypsin-dependent cleavage of agrin was concentrated at synapses, where the most heavily glycanated variants of agrin predominate. Because agrin has been shown to play an important role in the formation and the maintenance of excitatory synapses in the central nervous system, its local cleavage at the synapse implicates the neurotrypsin/agrin system in the regulation of adaptive reorganizations of the synaptic circuitry in the context of cognitive functions, such as learning and memory.

Calsyntenin-1 docks vesicular cargo to kinesin-1.

We identified a direct interaction between the neuronal transmembrane protein calsyntenin-1 and the light chain of Kinesin-1 (KLC1). GST pulldowns demonstrated that two highly conserved segments in the cytoplasmic domain of calsyntenin-1 mediate binding to the tetratricopeptide repeats of KLC1. A complex containing calsyntenin-1 and the Kinesin-1 motor was isolated from developing mouse brain and immunoelectron microscopy located calsyntenin-1 in association with tubulovesicular organelles in axonal fiber tracts. In primary neuronal cultures, calsyntenin-1-containing organelles were aligned along microtubules and partially colocalized with Kinesin-1. Using live imaging, we showed that these organelles are transported along axons with a velocity and processivity typical for fast axonal transport. Point mutations in the two kinesin-binding segments of calsyntenin-1 significantly reduced binding to KLC1 in vitro, and vesicles bearing mutated calsyntenin-1 exhibited a markedly altered anterograde axonal transport. In summary, our results indicate that calsyntenin-1 links a certain type of vesicular and tubulovesicular organelles to the Kinesin-1 motor.

Anatomical and Physiological Properties of GABAergic Neurotransmission in Organotypic Slice Cultures of Rat Hippocampus.

The anatomical and physiological properties of GABAergic inhibitory neurotransmission were investigated in organotypic slice cultures of rat hippocampus. Interneurons and terminal-like elements containing GABA-like immunoreactivity were numerous in tissue kept for 13 - 26 days in culture and showed a similar morphology and distribution to those known from investigations on the hippocampal formation in situ. Furthermore, after 8 - 30 days in culture, spontaneous and evoked IPSPs were observed in all CA3 pyramidal cells tested, resulting from an increase in chloride conductance, and were shown to be mediated by activation of GABA receptors. No functional decrement in the efficacy of GABAergic inhibitory synaptic transmission following chronic isolation and long-term maintenance in vitro was noticed. In particular, neither the magnitude of the synaptic conductance underlying the inhibitory postsynaptic currents nor its reversal potential varied with time in culture. Taken together, the present physiological and immunohistochemical data show that GABAergic inhibition is well expressed in organotypic hippocampal slice cultures and is maintained over periods of at least 4 weeks in vitro.

Glutamate-like Immunoreactivity in the Pigeon Optic Tectum and Effects of Retinal Ablation.

The pattern of glutamate-like immunoreactivity was investigated in the pigeon optic tectum. The most impressive aspect of the labelling pattern was an accumulation of immunoreactive terminal-like elements restricted to those superficial tectal layers that correspond to the termination zone of the retinal afferents. These immunoreactive puncta occurred frequently in small clusters. At the level of electron microscopy, many of the labelled nerve endings showed the characteristics of retinal terminals. Moreover, following unilateral retinal ablation a drastic loss of immunoreactive terminal-like puncta was observed in the retinorecipient layers of the tectum contralateral to the lesion. The remaining glutamate-immunoreactive terminal-like elements had the light and electron microscopic features typical of the afferents from the nucleus isthmi, pars parvocellularis (lpc). The relation between the latter result and the transmitter specificity of the afferents from this subtectal nucleus is unclear at present. On the other hand, the light and electron microscopic labelling patterns and the effect of retinal ablation suggest that afferents from retina and from lpc are the only major sources for glutamate-immunoreactive terminals in the pigeon optic tectum. Furthermore, the results are well in line with previous data indicating glutamate as neurotransmitter at least in part of the retinal afferents to the pigeon optic tectum.

Homocysteate, an Excitatory Transmitter Candidate Localized in Glia.

l-Homocysteate, a sulphur-containing l-glutamate analogue has recently been proposed as a neurotransmitter candidate. However, the cellular localization of this excitatory amino acid remained to be determined. By means of immunocytochemistry, the localization of homocysteate was accomplished in the cerebellar cortex of rats. Cerebellar glia could be defined as the major store of this compound. Homocysteate, thus, may not be a classical neurotransmitter but rather a member of another class of intercellular messengers that might be termed 'gliotransmitters'.

Spatial compartmentalization of AMPA glutamate receptor subunits at the calyx of Held synapse.

The mature calyx of Held ending on principal neurons of the medial nucleus of the trapezoid body (MNTB) has very specialized morphological and molecular features that make it possible to transmit auditory signals with high fidelity. In a previous work we described an increased localization of the ionotropic alpha-amino-3-hydroxy-5-methyl-4 isoxazolepropionic acid (AMPA) glutamate receptor (GluA) subunits at postsynaptic sites of the calyx of Held-principal cell body synapses from postnatal development to adult. The aim of the present study was to investigate whether the pattern of the synaptic distribution of GluA2/3/4c and -4 in adult MNTB principal cell bodies correlated with preferential subcellular domains (stalks and swellings) of the calyx. We used a postembedding immunocytochemical method combined with specific antibodies to GluA2/3/4c and GluA4 subunits. We found that the density of GluA2/3/4c in calyceal swellings (19 +/- 1.54 particles/microm) was higher than in stalks (10.93 +/- 1.37 particles/microm); however, the differences for GluA4 were not statistically significant (swellings: 13.84 +/- 1.39 particles/microm; stalks: 10.42 +/- 1.24 particles/microm). Furthermore, GluA2/3/4c and GluA4 labeling co-localized to some extent in calyceal stalks and swellings. Taking these data together, the distribution pattern of GluA subunits in postsynaptic specializations are indicative of a spatial compartmentalization of AMPA subunits in mature calyx-principal neuron synapses that may support the temporally precise transmission required for sound localization in the auditory brainstem.

Synaptic modifications at the CA3-CA1 synapse after chronic AMPA receptor blockade in rat hippocampal slices.

Maintenance of dendritic spines, the postsynaptic elements of most glutamatergic synapses in the central nervous system, requires continued activation of AMPA receptors. In organotypic hippocampal slice cultures, chronic blockade of AMPA receptors for 14 days induces a substantial loss of dendritic spines on CA1 pyramidal neurons. Here, using serial section electron microscopy, we show that loss of dendritic spines is paralleled by a significant reduction in synapse density. In contrast, we observed an increased number of asymmetric synapses onto the dendritic shaft, suggesting that spine retraction does not inevitably lead to synapse elimination. Functional analysis of the remaining synapses revealed that hippocampal circuitry compensates for the anatomical loss of synapses by increasing synaptic efficacy. Moreover, we found that the observed morphological and functional changes were associated with altered bidirectional synaptic plasticity. We conclude that continued activation of AMPA receptors is necessary for maintaining structure and function of central glutamatergic synapses.