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1.
Microcirculation ; 31(3): e12845, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38265175

RESUMEN

OBJECTIVE: The role of cerebral microvasculature in cognitive dysfunction can be investigated by identifying the impact of blood flow on cortical tissue oxygenation. In this paper, the impact of capillary stalls on microcirculatory characteristics such as flow and hematocrit (Ht) in the cortical angioarchitecture is studied. METHODS: Using a deterministic mathematical model to simulate blood flow in a realistic mouse cortex, hemodynamics parameters, including pressure, flow, vessel diameter-adjustable hematocrit, and transit time are calculated as a function of stalling events. RESULTS: Using a non-linear plasma skimming model, it is observed that Ht increases in the penetrating arteries from the pial vessels as a function of cortical depth. The incidence of stalling on Ht distribution along the blood network vessels shows reduction of RBCs around the tissue near occlusion sites and decreased Ht concentration downstream from the blockage points. Moreover, upstream of the occlusion, there is a noticeable increase of the Ht, leading to larger flow resistance due to higher blood viscosity. We predicted marked changes in transit time behavior due to stalls which match trends observed in mice in vivo. CONCLUSIONS: These changes to blood cell quantity and quality may be implicated in the development of Alzheimer's disease and contribute to the course of the illness.


Asunto(s)
Eritrocitos , Hemodinámica , Ratones , Animales , Microcirculación/fisiología , Hemodinámica/fisiología , Hematócrito , Eritrocitos/fisiología , Encéfalo
2.
Acta Biomater ; 140: 446-456, 2022 03 01.
Artículo en Inglés | MEDLINE | ID: mdl-34838701

RESUMEN

Introduction This study aims at identifying mechanical characteristics under bi-axial loading conditions of extracted swine pia mater (PM) and dura and arachnoid complex (DAC). Methods 59 porcine spinal samples have been tested on a bi-axial experimental device with a pre-load of 0.01 N and a displacement rate of 0.05 mm·s-1. Post-processing analysis included an elastic modulus, as well as constitutive model identification for Ogden model, reduced Gasser Ogden Holzapfel (GOH) model, anisotropic GOH model, transverse isotropic and anisotropic Gasser models as well as a Mooney-Rivlin model including fiber strengthening for PM. Additionally, micro-structure of the tissue was investigated using a bi-photon microscopy. Results Linear elastic moduli of 108 ± 40 MPa were found for DAC longitudinal direction, 53 ± 32 MPa for DAC circumferential direction, with a significant difference between directions (p < 0.001). PM presented significantly higher longitudinal than circumferential elastic moduli (26 ± 13 MPa vs 13 ± 9 MPa, p < 0.001). Transversely isotropic and anisotropic Gasser models were the most suited models for DAC (r2  =  0.99 and RMSE:0.4 and 0.3 MPa) and PM (r2 = 1 and RMSE:0.06 and 0.07 MPa) modelling. Conclusion This work provides reference values for further quasi-static bi-axial studies, and is the first for PM. Collagen structures observed by two photon microscopy confirmed the use of anisotropic Gasser model for PM and the existence of fenestration. The results from anisotropic Gasser model analysis depicted the best fit to experimental data as per this protocol. Further investigations are required to allow the use of meningeal tissue mechanical behaviour in finite element modelling with respect to physiological applications. STATEMENT OF SIGNIFICANCE: This study is the first to present biaxial tensile test of pia mater as well as constitutive model comparisons for dura and arachnoid complex tissue based on such tests. Collagen structures observed by semi-quantitative analysis of two photon microscopy confirmed the use of anisotropic Gasser model for pia mater and existence of fenestration. While clear identification of fibre population was not possible in DAC, results from anisotropic Gasser model depicted better fitting on experimental data as per this protocol. Bi-axial mechanical testing allows quasi-static characterization under conditions closer to the physiological context and the results presented could be used for further simulations of physiology. Indeed, the inclusion of meningeal tissue in finite element models will allow more accurate and reliable numerical simulations.


Asunto(s)
Aracnoides , Piamadre , Animales , Anisotropía , Fenómenos Biomecánicos , Módulo de Elasticidad , Estrés Mecánico , Porcinos , Resistencia a la Tracción
3.
J Mech Behav Biomed Mater ; 115: 104280, 2021 03.
Artículo en Inglés | MEDLINE | ID: mdl-33395616

RESUMEN

BACKGROUND: The spinal meninges play a mechanical protective role for the spinal cord. Better knowledge of the mechanical behavior of these tissues wrapping the cord is required to accurately model the stress and strain fields of the spinal cord during physiological or traumatic motions. Then, the mechanical properties of meninges along the spinal canal are not well documented. The aim of this study was to quantify the elastic meningeal mechanical properties along the porcine spinal cord in both the longitudinal direction and in the circumferential directions for the dura-arachnoid maters complex (DAC) and solely in the longitudinal direction for the pia mater. This analysis was completed in providing a range of isotropic hyperelastic coefficients to take into account the toe region. METHODS: Six complete spines (C0 - L5) were harvested from pigs (2-3 months) weighing 43±13 kg. The mechanical tests were performed within 12 h post mortem. A preload of 0.5 N was applied to the pia mater and of 2 N to the DAC samples, followed by 30 preconditioning cycles. Specimens were then loaded to failure at the same strain rate 0.2 mm/s (approximately 0.02/s, traction velocity/length of the sample) up to 12 mm of displacement. RESULTS: The following mean values were proposed for the elastic moduli of the spinal meninges. Longitudinal DAC elastic moduli: 22.4 MPa in cervical, 38.1 MPa in thoracic and 36.6 MPa in lumbar spinal levels; circumferential DAC elastic moduli: 20.6 MPa in cervical, 21.2 MPa in thoracic and 12.2 MPa in lumbar spinal levels; and longitudinal pia mater elastic moduli: 18.4 MPa in cervical, 17.2 MPa in thoracic and 19.6 MPa in lumbar spinal levels. DISCUSSION: The variety of mechanical properties of the spinal meninges suggests that it cannot be regarded as a homogenous structure along the whole length of the spinal cord.


Asunto(s)
Meninges , Médula Espinal , Animales , Duramadre , Módulo de Elasticidad , Piamadre , Estrés Mecánico , Porcinos
4.
Spine (Phila Pa 1976) ; 45(16): 1102-1109, 2020 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-32205694

RESUMEN

STUDY DESIGN: Continuous measurements and computation of absolute metrics of cervical subarachnoid space (CSS) and spinal cord (SC) geometries proposed are based on in vivo magnetic resonance imaging and 3D reconstruction. OBJECTIVE: The aim of the study is to offer a new methodology to continuously characterize and to quantify the detailed morphology of the CSS and the cervical SC in 3D for healthy subjects in both neutral supine and flexion. SUMMARY OF BACKGROUND DATA: To the best of our knowledge, no study provides a morphological quantification by absolute indices based on the 3D reconstruction of SC and CSS thanks to in vivo magnetic resonance imaging. Moreover, no study provides a continuous description of the geometries. METHODS: Absolute indices of SC (cross-sectional area, compression ratio, position in the canal, length) and of CSS (cross-sectional area, occupational ratio, lengths) were computed by measures from 3D semi-automatic reconstructions of high resolution in vivo magnetic resonance images (3D T2-SPACE sequence) on healthy subjects (N = 11) for two postures: supine neutral and flexion neck positions. The variability induced by the semi-automatic reconstruction and by the landmarks positioning were investigated by preliminary sensitivity analyses. Inter and intra-variability were also quantified on a randomly chosen part of our population (N = 5). RESULTS: The length and cross-sectional area of SC are significantly different (P < 0.05) in flexion compared with neutral neck position. Spinal cord stays centered in the canal for both postures. However, the cross-sectional area of CSS is submitted to low variation after C3 vertebra for both postures. Occupational ratio (OR) and compression ratio (CR) after C3 are significantly lower in flexion. CONCLUSION: This study presented interpretations of morphological measures: (1) left-right stability (described by the Left-Right eccentricity index) ensured by the denticulate ligaments and the nerve roots attached to the dural sheaths, (2) a Poisson effect of the SC was partially notified through its axial (antero-posterior [AP] diameter, OR, CR) and its longitudinal geometrical descriptions (length of spinal cord [LSC]). Such morphological data can be useful for geometrical finite element modeling and could now be used to compare with injured or symptomatic subjects. LEVEL OF EVIDENCE: 3.


Asunto(s)
Médula Cervical/anatomía & histología , Vértebras Cervicales/anatomía & histología , Imagen por Resonancia Magnética/métodos , Canal Medular/anatomía & histología , Médula Espinal/anatomía & histología , Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Cuello , Postura , Rango del Movimiento Articular
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