Independent and interactive effect of sedentary time and physical activity on risk of all-cause mortality: A prospective cohort study.

OBJECTIVES: Sedentary behavior (SB) and physical inactivity have been associated with an increased risk of all-cause mortality. Evidence in China is scarce, and it is unclear whether physical activity (PA) attenuates or even eliminates the harmful effects of prolonged SB in the Chinese population. METHODS: We conducted a prospective cohort study of 17 084 Chinese adults. PA and sitting time (ST) were assessed using the IPAQ. Cox proportional hazards models were used to estimate the risk of PA and ST with all-cause mortality. Interaction plots were used to visualize the interaction effects. RESULTS: During a median follow-up of 6.01 years, a total of 1106 deaths occurred. PA level was inversely associated with the incidence of all-cause mortality, while ST showed a detrimental association (all ptrend < 0.05). In the stratified analysis, ST was associated with all-cause mortality in the low PA, while the association was attenuated in the moderate PA group: the HRs (95% CI) comparing ST of 4-8, 8-11, and ≥11 to <4 h/day were 1.15 (0.73-1.81), 1.55 (0.92-2.59), and 2.70 (1.52-4.80), respectively. In the high PA group, no significant association was found across all ST levels. In the joint analysis, compared with the high PA and ST <4 h/day, the harmful effect was found only in the combined low PA and moderate PA groups with ST ≥11 h/day (HR:2.71, 95% CI:1.69-4.35). In addition, a significant interaction association was found. CONCLUSION: Our study, based on a prospective cohort, suggests that the detrimental effect of ST on all-cause mortality is attenuated or eliminated by high PA levels in the Chinese population.

Independent and joint associations of non-exercise cardiorespiratory fitness and obesity with risk of type 2 diabetes mellitus in the Rural Chinese Cohort Study.

BACKGROUND AND AIMS: An association between cardiorespiratory fitness (CRF) and type 2 diabetes mellitus (T2DM) has not been established in the Chinese population. This study aimed to estimate the independent and joint associations of CRF and obesity with T2DM incidence in the rural Chinese population. METHODS AND RESULTS: We conducted a prospective study of 11,825 non-T2DM subjects among rural Chinese adults. Cox regression models were used to estimate the independent and joint associations between CRF and obesity exposure on T2DM. Restricted cubic splines were used to model the dose-response association. During a median follow-up of 6.01 years, 835 participants developed T2DM. In comparison to quartile 1 of CRF, the multivariate hazard ratios (HRs) and 95% confidence intervals (CIs) of quartiles 2, 3, 4 were 0.75 (0.61-0.91), 0.54 (0.43-0.68), and 0.42 (0.32-0.55), respectively. When stratified by sex, the results were similar. Joint analyses showed that overweight/obesity-unfit individuals had a 2.28 times higher risk of developing T2DM than the normal weight-fit referent (HR 2.28, 95% CI 1.84-2.83; Pinteraction <0.001). The risk for the overweight/obesity-fit category (HR 1.61, 95% CI 1.21-2.15) was larger than for the normal weight-unfit category (HR 1.38, 95% CI 0.97-1.95) versus the normal weight-fit referent. Similar joint associations for waist circumference and CRF with T2DM were also observed. CONCLUSION: A negative association was observed between CRF and risk of T2DM. Overweight/obese or abdominal obesity and unfit participants showed the highest risks of T2DM. It is therefore strongly recommended that fitness-enhancing be encouraged for the prevention of T2DM, especially among obesity participants.

FOXA1 is upregulated in glioma and promotes proliferation as well as cell cycle through regulation of cyclin D1 expression.

INTRODUCTION: Forkhead box A1 (FOXA1) has been found to upregulate in numerous cancers, such as ovarian cancer and glioma. However, the detailed function of FOXA1 in glioma is still not known. The purpose of this study was to explore the underlying mechanism of FOXA1 in glioma cell progression. METHODS: The expressions of FOXA1 in glioma tissues and cells were determined using quantitative reverse transcription-polymerase chain reaction and western blotting assays. Wound healing assay and Transwell invasion assay were employed to detect the effects of FOXA1 on cellular migration and invasion. Cell Counting Kit-8 assay, colony formation assay, and flow cytometry analyses were also performed. RESULTS: Our study results suggested FOXA1 was upregulated in glioma tissues and cells and revealed that FOXA1 promoted glioma cellular proliferation by facilitating G1/S transition. Previous work has indicated that CCND1 expression is regulated by FOXA1 in ovarian cancer. ChIP and qChIP assay as well as dual luciferase reporter assay validated that CCND1 expression was also regulated by FOXA1 in glioma cells. Moreover, over-expression of CCND1 in siFOXA1-transfected cells partly offsets the effect of FOXA1 inhibition on cellular proliferation. CONCLUSION: FOXA1 promotes glioma cell progression, including cell proliferation and cell cycle, by targeting CCND1, and shows potential for the development of targeted treatment for glioma.

Effect of Resin Type on the Tribological Properties of a Three-Dimensional Self-Lubricating Composite Surface.

In this paper, three kinds of polymer, of epoxy resin (EP), phenolic resin (PF), and unsaturated polyester (UP), were used as fillers to prepare the laminated composite surface, and the tribological properties of a composite surface were studied under dry sliding condition. The results showed that: (i) the composites surface without MoS2 exhibited high friction coefficient and high wear rate at 25 °C, while the friction coefficients were reduced when the temperature increases to 100 °C; (ii) with the addition of MoS2, the friction coefficient of the epoxy resin composite containing MoS2 (E1) was below 0.22 under a temperature of 25⁻150 °C, and the friction coefficient was increased to 0.32 as temperature increased to 150 °C, while the average friction coefficient of the unsaturated polyester composite containing MoS2 (U1) was very low and below 0.20 under a temperature of 25⁻150 °C. Analysis of the wear scars indicated that, for the MoS2-containing composite, the transfer films of the E1 and U1 were smooth and continuous under low temperature, while the transfer film of U1 was comparatively complete than that of E1 under 150 °C. The composites with solid lubrication had excellent high-temperature self-lubricating properties, which was attributed to the synergistic effect of the laminated structure, and the thermal expansion of the polymer, and finally a transfer film was formed on the sliding path.