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1.
J Am Chem Soc ; 145(43): 23600-23608, 2023 11 01.
Artículo en Inglés | MEDLINE | ID: mdl-37871253

RESUMEN

The introduction of noncanonical amino acids into proteins and peptides has been of great interest for many years and has facilitated the detailed study of peptide/protein structure and mechanism. In addition to numerous nonproteinogenic α-l-amino acids, bacterial ribosome modification has provided the wherewithal to enable the synthesis of peptides and proteins with a much greater range of structural diversity, as has the use of endogenous bacterial proteins in reconstituted protein synthesizing systems. In a recent report, elongation factor P (EF-P), putatively essential for enabling the incorporation of contiguous proline residues into proteins, was shown to facilitate the introduction of an N-methylated amino acid in addition to proline. This finding prompted us to investigate the properties of this protein factor with a broad variety of structurally diverse amino acid analogues using an optimized suppressor tRNAPro that we designed. While these analogues can generally be incorporated into proteins only in systems containing modified ribosomes specifically selected for their incorporation, we found that EF-P could significantly enhance their incorporation into model protein dihydrofolate reductase using wild-type ribosomes. Plausibly, the increased yields observed in the presence of structurally diverse amino acid analogues may result from the formation of a stabilized ribosomal complex in the presence of EF-P that provides more favorable conditions for peptide bond formation. This finding should enable the facile incorporation of a much broader structural variety of amino acid analogues into proteins and peptides using native ribosomes.


Asunto(s)
Aminoácidos , Escherichia coli , Aminoácidos/química , Escherichia coli/metabolismo , Tetrahidrofolato Deshidrogenasa/metabolismo , Factores de Elongación de Péptidos/metabolismo , Péptidos/química , Prolina/metabolismo
2.
Bioorg Med Chem ; 41: 116210, 2021 07 01.
Artículo en Inglés | MEDLINE | ID: mdl-34022527

RESUMEN

The elaboration of peptides and proteins containing non-proteinogenic amino acids has been realized using several complementary strategies, including chemical synthesis, ribosome- or non-ribosome-mediated elaboration, intein-mediated polypeptide rearrangements, or some combination of these strategies. All of these have strengths and limitations, and significant efforts have been focused on minimizing the effects of limitations, to improve the overall utility of individual strategies. Our laboratory has studied ribosomally mediated peptide and protein synthesis involving a wide variety of non-proteinogenic amino acids, and in recent years we have described a novel strategy for the selection of modified bacterial ribosomes. These modified ribosomes have enabled the incorporation into peptides and proteins of numerous modified amino acids not accessible using wild-type ribosomes. This has included d-amino acids, ß-amino acids, dipeptides and dipeptidomimetic species, as well as phosphorylated amino acids. Presently, we have considered novel strategies for incorporating non-proteinogenic amino acids in improved yields. This has included the incorporation of non-proteinogenic amino acids into contiguous positions, a transformation known to be challenging. We demonstrate the preparation of this type of protein modification by utilizing a suppressor tRNACUA activated with a dipeptide consisting of two identical non-proteinogenic amino acids, in the presence of modified ribosomes selected to recognize such dipeptides. Also, we demonstrate that the use of bis-aminoacylated suppressor tRNAs, shown previously to increase protein yields significantly in vitro, can be extended to the use of non-proteinogenic amino acids.


Asunto(s)
Dipéptidos/química , Proteínas/síntesis química , Aminoácidos/química , Escherichia coli , Conformación Proteica , ARN Mensajero/química , ARN Mensajero/genética , ARN Mensajero/metabolismo , ARN de Transferencia/química , Ribosomas
3.
J Am Chem Soc ; 139(13): 4611-4614, 2017 04 05.
Artículo en Inglés | MEDLINE | ID: mdl-28263595

RESUMEN

Several variants of a nucleic acid binding motif (RRM1) of putative transcription factor hnRNP LL containing nucleobase amino acids at specific positions have been prepared and used to study binding affinity for the BCL2 i-motif DNA. Molecular modeling suggested a number of amino acids in RRM1 likely to be involved in interaction with the i-motif DNA, and His24 and Arg26 were chosen for modification based on their potential ability to interact with G14 of the i-motif DNA. Four nucleobase amino acids were introduced into RRM1 at one or both of positions 24 and 26. The introduction of cytosine nucleobase 2 into position 24 of RRM1 increased the affinity of the modified protein for the i-motif DNA, consistent with the possible Watson-Crick interaction of 2 and G14. In comparison, the introduction of uracil nucleobase 3 had a minimal effect on DNA affinity. Two structurally simplified nucleobase analogues (1 and 4) lacking both the N-1 and the 2-oxo substituents were also introduced in lieu of His24. Again, the RRM1 analogue containing 1 exhibited enhanced affinity for the i-motif DNA, while the protein analogue containing 4 bound less tightly to the DNA substrate. Finally, the modified protein containing 1 in lieu of Arg26 also bound to the i-motif DNA more strongly than the wild-type protein, but a protein containing 1 both at positions 24 and 26 bound to the DNA less strongly than wild type. The results support the idea of using nucleobase amino acids as protein constituents for controlling and enhancing DNA-protein interaction. Finally, modification of the i-motif DNA at G14 diminished RRM1-DNA interaction, as well as the ability of nucleobase amino acid 1 to stabilize RRM1-DNA interaction.


Asunto(s)
Aminoácidos/química , ADN/química , Proteínas Proto-Oncogénicas c-bcl-2/química , Sitios de Unión , Humanos , Modelos Moleculares , Estructura Molecular , Motivos de Nucleótidos
4.
Biochemistry ; 55(17): 2427-40, 2016 May 03.
Artículo en Inglés | MEDLINE | ID: mdl-27050631

RESUMEN

Described herein are the synthesis and photophysical characterization of a library of aryl-substituted oxazole- and thiazole-based dipeptidomimetic analogues, and their incorporation into position 66 of green fluorescent protein (GFP) in lieu of the natural fluorophore. These fluorescent analogues resemble the fluorophore formed naturally by GFP. As anticipated, the photophysical properties of the analogues varied as a function of the substituents at the para position of the phenyl ring. The fluorescence emission wavelength maxima of compounds in the library varied from ∼365 nm (near-UV region) to ∼490 nm (visible region). The compounds also exhibited a large range of quantum yields (0.01-0.92). The analogues were used to activate a suppressor tRNACUA and were incorporated into position 66 of GFP using an in vitro protein biosynthesizing system that employed engineered ribosomes selected for their ability to incorporate dipeptides. Four analogues with interesting photophysical properties and reasonable suppression yields were chosen, and the fluorescent proteins (FPs) containing these fluorophores were prepared on a larger scale for more detailed study. When the FPs were compared with the respective aminoacyl-tRNAs and the actual dipeptide analogues, the FPs exhibited significantly enhanced fluorescence intensities at the same concentrations. Part of this was shown to be due to the presence of the fluorophores as an intrinsic element of the protein backbone. There were also characteristic shifts in the emission maxima, indicating the environmental sensitivity of these probes. Acridon-2-ylalanine and oxazole 1a were incorporated into positions 39 and 66 of GFP, respectively, and were shown to form an efficient Förster resonance energy transfer (FRET) pair, demonstrating that the analogues can be used as FRET probes.


Asunto(s)
Dipéptidos/metabolismo , Escherichia coli/metabolismo , Fluorescencia , Peptidomiméticos/síntesis química , Peptidomiméticos/metabolismo , ARN de Transferencia/metabolismo , Ribosomas/metabolismo , Dipéptidos/síntesis química , Transferencia Resonante de Energía de Fluorescencia , Colorantes Fluorescentes , Proteínas Fluorescentes Verdes , Humanos , Modelos Moleculares , Estructura Molecular , Biosíntesis de Proteínas
5.
J Am Chem Soc ; 138(34): 10950-62, 2016 08 31.
Artículo en Inglés | MEDLINE | ID: mdl-27483029

RESUMEN

The recently discovered role of the BCL2 (B-cell lymphoma 2 gene) promoter i-motif DNA in modulation of gene expression via interaction with the ribonucleoprotein hnRNP L-like (hnRNP LL) has prompted a more detailed study of the nature of this protein-DNA interaction. The RNA recognition motifs (RRMs) of hnRNP LL were expressed individually, and both RRM1 and RRM2 were found to bind efficiently to the BCL2 i-motif DNA, as well as being critical for transcriptional activation, whereas RRM3-4 bound only weakly to this DNA. Binding was followed by unfolding of the DNA as monitored by changes in the CD spectrum. Mutational analysis of the i-motif DNA revealed that binding involved primarily the lateral loops of the i-motif. The kinetics of binding of the DNA with RRM1 was explored by recording CD spectra at predetermined times following admixture of the protein and DNA. The change in molar ellipticity was readily apparent after 30 s and largely complete within 1 min. A more detailed view of protein-DNA interaction was obtained by introducing the fluorescence donor 6-CNTrp in RRM1 at position 137, and the acceptor 4-aminobenzo[g]quinazoline-2-one (Cf) in lieu of cytidine22 in the i-motif DNA. The course of binding of the two species was monitored by FRET, which reflected a steady increase in energy transfer over a period of several minutes. The FRET signal could be diminished by the further addition of (unlabeled) RRM2, no doubt reflecting competition for binding to the i-motif DNA. These experiments using the individual RRM domains from hnRNP LL confirm the role of this transcription factor in activation of BCL2 transcription via the i-motif in the promoter element.


Asunto(s)
ADN/genética , ADN/metabolismo , Ribonucleoproteínas Nucleares Heterogéneas/química , Ribonucleoproteínas Nucleares Heterogéneas/metabolismo , Motivos de Nucleótidos , Regiones Promotoras Genéticas/genética , Proteínas Proto-Oncogénicas c-bcl-2/genética , Secuencia de Bases , ADN/química , Modelos Moleculares , Unión Proteica , Dominios Proteicos
6.
Bioorg Med Chem ; 24(18): 4177-4187, 2016 09 15.
Artículo en Inglés | MEDLINE | ID: mdl-27452282

RESUMEN

Proteins which bind to nucleic acids and regulate their structure and functions are numerous and exceptionally important. Such proteins employ a variety of strategies for recognition of the relevant structural elements in their nucleic acid substrates, some of which have been shown to involve rather subtle interactions which might have been difficult to design from first principles. In the present study, we have explored the preparation of proteins containing unnatural amino acids having nucleobase side chains. In principle, the introduction of multiple nucleobase amino acids into the nucleic acid binding domain of a protein should enable these modified proteins to interact with their nucleic acid substrates using Watson-Crick and other base pairing interactions. We describe the synthesis of five alanyl nucleobase amino acids protected in a fashion which enabled their attachment to a suppressor tRNA, and their incorporation into each of two proteins with acceptable efficiencies. The nucleobases studied included cytosine, uracil, thymine, adenine and guanine, i.e. the major nucleobase constituents of DNA and RNA. Dihydrofolate reductase was chosen as one model protein to enable direct comparison of the facility of incorporation of the nucleobase amino acids with numerous other unnatural amino acids studied previously. The Klenow fragment of DNA polymerase I was chosen as a representative DNA binding protein whose mode of action has been studied in detail.


Asunto(s)
Alanina/análogos & derivados , Alanina/síntesis química , Purinas/síntesis química , Pirimidinonas/síntesis química , Tetrahidrofolato Deshidrogenasa/química , Alanina/genética , Codón , ADN Polimerasa I/genética , Escherichia coli , ARN Mensajero/química , ARN Mensajero/genética , ARN de Transferencia/síntesis química , ARN de Transferencia/genética , Tetrahidrofolato Deshidrogenasa/genética , Aminoacilación de ARN de Transferencia
7.
Biochemistry ; 54(51): 7457-69, 2015 Dec 29.
Artículo en Inglés | MEDLINE | ID: mdl-26618501

RESUMEN

Described herein are the syntheses and photophysical characterization of three novel cyanotryptophans, and their efficient incorporation into proteins as fluorescent probes. Photophysical characteristics indicated that each was significantly brighter and red-shifted in fluorescence emission relative to tryptophan. Each analogue was used to activate a suppressor tRNA transcript and was incorporated with good efficiency into two different positions (Trp22 and Trp74) of Escherichia coli dihydrofolate reductase (ecDHFR). The Trp analogues could be monitored selectively in the presence of multiple native Trp residues in DHFR. 6-CNTrp (A) formed an efficient Förster resonance energy transfer (FRET) pair with l-(7-hydroxycoumarin-4-yl)ethylglycine (HCO, D) at position 17. Further, 6-CNTrp (A) was incorporated into two DNA binding proteins, including the Klenow fragment of DNA polymerase I and an RNA recognition motif (RRM2) of heterogeneous nuclear ribonucleoprotein L-like (hnRNP LL). Using these proteins, we demonstrated the use of FRET involving A as a fluorescence donor and benzo[g]quinazoline-2,4-(1H,3H)-dione 2'-deoxyriboside (Tf) or 4-aminobenzo[g]quinazoline-2-one 2'-deoxyriboside (Cf) as fluorescent acceptors to study the binding interaction of the Klenow fragment with duplex DNA oligomers (labeled with Tf), or the domain-specific association between hnRNP LL and the BCL2 i-motif DNA (labeled with Cf). Thus, the non-natural amino acid could be used as a FRET partner for studying protein-nucleic acid interactions. Together, these findings demonstrate the potential utility of 6-CNTrp (A) as a fluorescence donor for the study of protein conformational events.


Asunto(s)
ADN/química , Colorantes Fluorescentes/química , Proteínas/química , Triptófano/análogos & derivados , Transferencia Resonante de Energía de Fluorescencia , Unión Proteica , Conformación Proteica , Triptófano/química
8.
Bioorg Med Chem Lett ; 25(6): 1182-5, 2015 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-25704889

RESUMEN

A fluorescently modified CD4 domain 1 (mD1) protein has been designed and elaborated in an in vitro expression system. This fluorescent probe contains a Förster resonance energy transfer (FRET) pair, which uses a tryptophan residue as the fluorescence donor and an acridon-2-ylalanine (Acd) as the acceptor. When excited at 260nm, energy was transferred from tryptophan to the Acd residue of mD1, and emitted fluorescence at 420nm. This fluoresence was quenched after Evans blue (EB) inhibitor or HIV-1 gp120 protein binding, presumably as a consequence of changes in the distance and dipole orientation between the donor and acceptor; the emission intensity at 420nm decreased in a concentration-dependent fashion. This fluorescent CD4 probe could be developed into a novel tool for HIV-1 gp120 protein detection. It also could be used to screen small molecules that inhibit the gp120-CD4 interaction.


Asunto(s)
Antígenos CD4/química , Transferencia Resonante de Energía de Fluorescencia , Colorantes Fluorescentes/química , Proteína gp120 de Envoltorio del VIH/análisis , VIH-1/metabolismo , Antígenos CD4/metabolismo , Humanos , Unión Proteica , Estructura Terciaria de Proteína , Triptófano/química
9.
Bioorg Med Chem Lett ; 24(24): 5699-5703, 2014 Dec 15.
Artículo en Inglés | MEDLINE | ID: mdl-25453804

RESUMEN

CD4-gp120 interaction is the first step for HIV-1 entry into host cells. A highly conserved pocket in gp120 protein is an attractive target for developing gp120 inhibitors or novel HIV detection tools. Here we incorporate seven phenylalanine derivatives having different sizes and steric conformations into position 43 of domain 1 of CD4 (mD1.2) to explore the architecture of the 'Phe43 cavity' of HIV-1 gp120. The results show that the conserved hydrophobic pocket in gp120 tolerates a hydrophobic side chain of residue 43 of CD protein, which is 12.2 Å in length and 8.0 Å in width. This result provides useful information for developing novel gp120 inhibitors or new HIV detection tools.


Asunto(s)
Antígenos CD4/química , Antígenos CD4/metabolismo , Proteína gp120 de Envoltorio del VIH/química , Proteína gp120 de Envoltorio del VIH/metabolismo , Fenilalanina/química , Fenilalanina/metabolismo , Sitios de Unión , Humanos , Modelos Moleculares , Estructura Molecular , Conformación Proteica
10.
Bioorg Med Chem ; 22(21): 5924-34, 2014 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-25284250

RESUMEN

With the continuing interest in deciphering the interplay between protein function and conformational changes, small fluorescence probes will be especially useful for tracking changes in the crowded protein interior space. Presently, we describe the potential utility of six unnatural amino acid fluorescence donors structurally related to tryptophan and show how they can be efficiently incorporated into a protein as fluorescence probes. We also examine the various photophysical properties of the new Trp analogues, which are significantly redshifted in their fluorescence spectra relative to tryptophan. In general, the Trp analogues were well tolerated when inserted into Escherichia coli DHFR, and did not perturb enzyme activity, although substitution for Trp22 did result in a diminution in DHFR activity. Further, it was demonstrated that D and E at position 37 formed efficient FRET pairs with acridon-2-ylalanine (Acd) at position 17. The same was also true for a DHFR construct containing E at position 79 and Acd at position 17. Together, these findings demonstrate that these tryptophan analogues can be introduced into DHFR with minimal disruption of function, and that they can be employed for the selective study of targeted conformational changes in proteins, even in the presence of unmodified tryptophans.


Asunto(s)
Escherichia coli/enzimología , Colorantes Fluorescentes/química , Tetrahidrofolato Deshidrogenasa/química , Triptófano/análogos & derivados , Escherichia coli/química , Transferencia Resonante de Energía de Fluorescencia , Modelos Moleculares , Conformación Proteica
11.
Sci Immunol ; 7(78): eadd3075, 2022 12 09.
Artículo en Inglés | MEDLINE | ID: mdl-36459542

RESUMEN

Respiratory tract resident memory T cells (TRM), typically generated by local vaccination or infection, can accelerate control of pulmonary infections that evade neutralizing antibody. It is unknown whether mRNA vaccination establishes respiratory TRM. We generated a self-amplifying mRNA vaccine encoding the influenza A virus nucleoprotein that is encapsulated in modified dendron-based nanoparticles. Here, we report how routes of immunization in mice, including contralateral versus ipsilateral intramuscular boosts, or intravenous and intranasal routes, influenced influenza-specific cell-mediated and humoral immunity. Parabiotic surgeries revealed that intramuscular immunization was sufficient to establish CD8 TRM in the lung and draining lymph nodes. Contralateral, compared with ipsilateral, intramuscular boosting broadened the distribution of lymph node TRM and T follicular helper cells but slightly diminished resulting levels of serum antibody. Intranasal mRNA delivery established modest circulating CD8 and CD4 T cell memory but augmented distribution to the respiratory mucosa. Combining intramuscular immunizations with an intranasal mRNA boost achieved high levels of both circulating T cell memory and lung TRM. Thus, routes of mRNA vaccination influence humoral and cell-mediated immunity, and intramuscular prime-boosting establishes lung TRM that can be further expanded by an additional intranasal immunization.


Asunto(s)
Linfocitos T CD4-Positivos , Vacunación , Animales , Ratones , ARN Mensajero , Anticuerpos Neutralizantes , Linfocitos T CD8-positivos , Vacunas de ARNm
12.
ACS Infect Dis ; 6(7): 1827-1835, 2020 07 10.
Artículo en Inglés | MEDLINE | ID: mdl-32369341

RESUMEN

The emergence of artemisinin resistance, combined with certain suboptimal properties of ozonide agents arterolane and artefenomel, has necessitated the search for new drug candidates in the endoperoxide class. Our group has focused on trioxolane analogues with substitution patterns not previously explored. Here, we describe the enantioselective synthesis of analogues bearing a trans-3″ carbamate side chain and find these to be superior, both in vitro and in vivo, to the previously reported amides. We identified multiple analogues that surpass the oral efficacy of arterolane in the Plasmodium berghei model while exhibiting drug-like properties (logD, solubility, metabolic stability) similar or superior to next-generation clinical candidates like E209 and OZ609. While the preclinical assessment of new analogues is still underway, current data suggest the potential of this chemotype as a likely source of future drug candidates from the endoperoxide class.


Asunto(s)
Antimaláricos , Preparaciones Farmacéuticas , Antimaláricos/farmacología , Plasmodium berghei , Plasmodium falciparum
13.
ACS Infect Dis ; 5(8): 1366-1375, 2019 08 09.
Artículo en Inglés | MEDLINE | ID: mdl-31140267

RESUMEN

Iron is essential to all life, and competition for this vital nutrient is central to host-pathogen interactions during infection. The opportunistic Gram-negative pathogen Pseudomonas aeruginosa utilizes a diverse array of iron-acquisition strategies, including those enabling import of extracellular ferrous iron. We hypothesize that soluble and redox-active ferrous iron can be employed to activate caged antibiotics at sites of infection in vivo. Here we describe new chemistry that expands the application of our laboratory's Fe2+-activated-prodrug chemistry to cage hydroxamic acids, a class of drugs that present manifold development challenges. We synthesize the caged form of a known LpxC inhibitor and show that it is efficacious in an acute P. aeruginosa mouse-lung infection model, despite showing little activity in cell-culture experiments. Overall, our results are consistent with the Fe2+-promoted uncaging of an antibacterial payload at sites of infection in an animal and lend support to recent reports indicating that extracellular pools of ferrous iron can be utilized by bacterial pathogens like P. aeruginosa during infection.


Asunto(s)
Amidohidrolasas/antagonistas & inhibidores , Antibacterianos/uso terapéutico , Inhibidores Enzimáticos/uso terapéutico , Compuestos Ferrosos/uso terapéutico , Pseudomonas aeruginosa/efectos de los fármacos , Animales , Antibacterianos/química , Inhibidores Enzimáticos/química , Femenino , Compuestos Ferrosos/química , Bacterias Gramnegativas/efectos de los fármacos , Interacciones Huésped-Patógeno/efectos de los fármacos , Ácidos Hidroxámicos/metabolismo , Pulmón/microbiología , Ratones , Profármacos/administración & dosificación , Pseudomonas aeruginosa/patogenicidad , Infecciones del Sistema Respiratorio/tratamiento farmacológico , Infecciones del Sistema Respiratorio/microbiología
14.
Org Lett ; 16(2): 556-9, 2014 Jan 17.
Artículo en Inglés | MEDLINE | ID: mdl-24392870

RESUMEN

Two new fluorescent probes of protein structure and dynamics have been prepared by concise asymmetric syntheses using the Schöllkopf chiral auxiliary. The site-specific incorporation of one probe into dihydrofolate reductase is reported. The utility of these tryptophan derivatives lies in their absorption and emission maxima which differ from those of tryptophan, as well as in their large Stokes shifts and high molar absorptivities.


Asunto(s)
Colorantes Fluorescentes/síntesis química , Triptófano/análogos & derivados , Triptófano/síntesis química , Colorantes Fluorescentes/química , Estructura Molecular , Procesos Fotoquímicos , Triptófano/química
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