RESUMEN
In recent years, EEG microstate analysis has attracted much attention as a tool for characterizing the spatial and temporal dynamics of large-scale electrophysiological activities in the human brain. Canonical 4 states (classes A, B, C, and D) have been widely reported, and they have been pointed out for their relationships with cognitive functions and several psychiatric disorders such as schizophrenia, in particular, through their static parameters such as average duration, occurrence, coverage, and transition probability. However, the relationships between event-related microstate changes and their related cognitive functions, as is often analyzed in event-related potentials under time-locked frameworks, is still not well understood. Furthermore, not enough attention has been paid to the relationship between microstate dynamics and static characteristics. To clarify the relationships between the static microstate parameters and dynamic microstate changes, and between the dynamics and working memory (WM) function, we first examined the temporal profiles of the microstates during the N-back task. We found significant event-related microstate dynamics that differed predominantly with WM loads, which were not clearly observed in the static parameters. Furthermore, in the 2-back condition, patterns of state transitions from class A to C in the high- and low-performance groups showed prominent differences at 50-300 ms after stimulus onset. We also confirmed that the transition patterns of the specific time periods were able to predict the performance level (low or high) in the 2-back condition at a significant level, where a specific transition between microstates, namely from class A to C with specific polarity, contributed to the prediction robustly. Taken together, our findings indicate that event-related microstate dynamics at 50-300 ms after onset may be essential for WM function. This suggests that event-related microstate dynamics can reflect more highly-refined brain functions.
Asunto(s)
Electroencefalografía , Memoria a Corto Plazo , Humanos , Encéfalo/fisiología , Cognición , Mapeo EncefálicoRESUMEN
Pain is a complex experience composed of sensory and affective components. Although the neural systems of the sensory component of pain have been studied extensively, those of its affective component remain to be determined. In the present study, we examined the effects of corticotropin-releasing factor (CRF) and neuropeptide Y (NPY) injected into the dorsolateral bed nucleus of the stria terminalis (dlBNST) on pain-induced aversion and nociceptive behaviors in rats to examine the roles of these peptides in affective and sensory components of pain, respectively. In vivo microdialysis showed that formalin-evoked pain enhanced the release of CRF in this brain region. Using a conditioned place aversion (CPA) test, we found that intra-dlBNST injection of a CRF1 or CRF2 receptor antagonist suppressed pain-induced aversion. Intra-dlBNST CRF injection induced CPA even in the absence of pain stimulation. On the other hand, intra-dlBNST NPY injection suppressed pain-induced aversion. Coadministration of NPY inhibited CRF-induced CPA. This inhibitory effect of NPY was blocked by coadministration of a Y1 or Y5 receptor antagonist. Furthermore, whole-cell patch-clamp electrophysiology in dlBNST slices revealed that CRF increased neuronal excitability specifically in type II dlBNST neurons, whereas NPY decreased it in these neurons. Excitatory effects of CRF on type II dlBNST neurons were suppressed by NPY. These results have uncovered some of the neuronal mechanisms underlying the affective component of pain by showing opposing roles of intra-dlBNST CRF and NPY in pain-induced aversion and opposing actions of these peptides on neuronal excitability converging on the same target, type II neurons, within the dlBNST.
Asunto(s)
Síntomas Afectivos/tratamiento farmacológico , Hormona Liberadora de Corticotropina/efectos adversos , Hormonas/efectos adversos , Neuropéptido Y/uso terapéutico , Dolor/complicaciones , Núcleos Septales/efectos de los fármacos , Potenciales de Acción/efectos de los fármacos , Síntomas Afectivos/etiología , Análisis de Varianza , Compuestos de Anilina/farmacología , Animales , Arginina/análogos & derivados , Arginina/farmacología , Hormona Liberadora de Corticotropina/agonistas , Hormona Liberadora de Corticotropina/antagonistas & inhibidores , Ciclohexanos/farmacología , Modelos Animales de Enfermedad , Antagonistas de Aminoácidos Excitadores/farmacología , Formaldehído/toxicidad , Antagonistas del GABA/farmacología , Antagonistas de Hormonas/farmacología , Hormonas/agonistas , Técnicas In Vitro , Ácido Quinurénico/farmacología , Masculino , Potenciales de la Membrana/efectos de los fármacos , Microdiálisis , Neuronas/efectos de los fármacos , Neuropéptido Y/agonistas , Neuropéptido Y/antagonistas & inhibidores , Dolor/inducido químicamente , Dimensión del Dolor , Fragmentos de Péptidos/farmacología , Piridazinas/farmacología , Pirimidinas/farmacología , Ratas , Ratas Sprague-Dawley , Núcleos Septales/citología , Núcleos Septales/fisiología , Xantenos/farmacologíaRESUMEN
Diffuse noxious inhibitory control (DNIC) is a phenomenon to reflect descending pain modulation in animals. Conditioned pain modulation (CPM) is the human counterpart of DNIC and is reduced in patients with several chronic pain conditions. Duloxetine is a serotonin and noradrenaline reuptake inhibitor that ameliorates CPM impairment in patients with diabetic neuropathy. Although some studies have reported the effects of different pharmacological agents on CPM, few studies have compared the effects of some analgesics in both humans and rodents. Therefore, we established a stable evaluation method for DNIC in rats and determined whether duloxetine and other specific analgesics affect DNIC impairment in rat models of peripheral neuropathic pain and osteoarthritis pain, two types of chronic pain. As a conditioning stimulus, capsaicin was injected into the forepaw of rats. The paw withdrawal threshold (PWT) in response to mechanical pressure was measured for the hindpaw. Peripheral neuropathic pain and osteoarthritis pain models were developed by partial sciatic nerve ligation (PSNL) and the intra-articular injection of 2â¯mg monoiodoacetate (MIA), respectively. Capsaicin (30-100⯵g/site) increased the PWT, in a dose-dependent manner, in naive rats. The threshold significantly increased at 30⯵g and reached its maximal level at 100⯵g. The change in PWT following capsaicin injection was significantly reduced in PSNL-treated rats, but the threshold was increased by the subcutaneous administration of duloxetine (10â¯mg/kg). The oral administrations of pregabalin (10â¯mg/kg) and celecoxib (3â¯mg/kg) did not affect the PWT in PSNL-treated rats. Similarly, MIA-injected rats also showed a reduced change in PWT following capsaicin injection. Duloxetine, but not pregabalin and celecoxib, significantly increased the PWT in MIA-injected rats. These results suggested that duloxetine can directly ameliorate DNIC impairment in rat models of chronic pain. Duloxetine may be useful for modulating chronic pain by restoring function to the endogenous, descending, inhibitory pathway.
Asunto(s)
Analgésicos/farmacología , Dolor Crónico/tratamiento farmacológico , Control Inhibidor Nocivo Difuso/efectos de los fármacos , Clorhidrato de Duloxetina/farmacología , Osteoartritis de la Rodilla/tratamiento farmacológico , Animales , Control Inhibidor Nocivo Difuso/fisiología , Masculino , Neuralgia/tratamiento farmacológico , Neuralgia/fisiopatología , Dimensión del Dolor/efectos de los fármacos , Pregabalina/farmacología , Ratas Sprague-DawleyRESUMEN
Microglia exhibit various activation phenotypes in the spinal cord after peripheral nerve injury, and promote neuropathic pain. Ibudilast is a phosphodiesterase inhibitor with anti-inflammatory activity, but its effect on activated microglia in chronic neuropathic pain is poorly understood. We investigated whether ibudilast was effective on established allodynia associated with activated microglial phenotypes in two rat models of peripheral and central neuropathic pain. A single intrathecal injection of ibudilast (25⯵g) inhibited established allodynia on days 7-21 after sciatic nerve injury in rats. Repeated injections of ibudilast (25⯵g/day) reduced the numbers of phosphorylated p38-positive cells without changing hypertrophic microglia, whereas minocycline (100⯵g/day) decreased the numbers of hypertrophic microglia associated with phosphorylated p38 levels in the spinal cord. Gene analysis revealed that minocycline, but not ibudilast, increased the expression of anti-inflammatory cytokine genes Il10 and Tgfß1 in the spinal cord. Propentofylline (100⯵g/day) was less effective on microglial phenotypes and established allodynia. Ibudilast inhibited persistent allodynia after the recovery of motor deficits in experimental autoimmune encephalomyelitis rats. Therefore, ibudilast might be effective for chronic neuropathic pain after peripheral and central nerve damage. Ibudilast mediated these effects on activated microglia using a different mechanism compared with minocycline and propentofylline.
Asunto(s)
Hiperalgesia/tratamiento farmacológico , Microglía/efectos de los fármacos , Neuralgia/tratamiento farmacológico , Inhibidores de Fosfodiesterasa/farmacología , Piridinas/farmacología , Animales , Encefalomielitis Autoinmune Experimental/complicaciones , Encefalomielitis Autoinmune Experimental/etiología , Femenino , Humanos , Hiperalgesia/etiología , Inyecciones Espinales , Masculino , Minociclina/farmacología , Neuralgia/etiología , Fármacos Neuroprotectores/farmacología , Dimensión del Dolor , Traumatismos de los Nervios Periféricos/complicaciones , Traumatismos de los Nervios Periféricos/etiología , Inhibidores de Fosfodiesterasa/uso terapéutico , Fosforilación , Piridinas/uso terapéutico , Ratas , Ratas Endogámicas Lew , Ratas Sprague-Dawley , Nervio Ciático/lesiones , Médula Espinal/citología , Xantinas/farmacología , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismoRESUMEN
Dysfunction in the central serotonin (5-HT) and norepinephrine (NE) systems cause depression and pain. Descending spinal pain modulatory pathways are important in the analgesic mechanisms of antidepressants, particularly serotonin and norepinephrine reuptake inhibitors (SNRIs). While many non-clinical studies have demonstrated the roles of central monoaminergic systems in pain, there is little evidence to illuminate the direct contribution of spinal descending pain modulatory systems independently of depressive-like behavior. To examine the effects of dysfunction of spinal monoaminergic systems on pain sensitivity, we established a rat chronic pain model by administering lumbar-intrathecal reserpine to minimize its influence on brain. Lumbar-intrathecal reserpine evoked persistent mechanical hypersensitivity and corresponding reductions in spinal 5-HT and NE concentrations (from 767.2 to 241.6ng/g and from 455.9 to 41.7ng/g, respectively after reserpine 30nmol). Lumbar-intrathecal reserpine did not deplete brain monoamines or bring about depressive-like behavior in the forced swim test. Spinal monoamines depletion-induced pain sensitivity was ameliorated by lumbar-intrathecal administration of the SNRIs (duloxetine and milnacipran) in dose-dependent manners. These suggest that increased pain sensitivity could be induced by dysfunction solely of the descending pain modulatory system, regardless of depressive-like behavior, and lumbar-intrathecal administration of SNRIs could ameliorate the pain sensitivity which might be mediated by affecting the descending pain modulatory system in the spinal cord, not via their antidepressant effects.