RESUMEN
A lysosome-targeting dual-functional fluorescent probe was rationally designed and developed for imaging intracellular lysosomal viscosity and beta-amyloid. More importantly, the real-time tracking of the dynamic movement of lysosomes, as vesicle structures, has been achieved using Lyso-MC.
Asunto(s)
Péptidos beta-Amiloides/metabolismo , Colorantes Fluorescentes/química , Lisosomas/metabolismo , Péptidos beta-Amiloides/química , Animales , Línea Celular , Humanos , Lisosomas/química , Microscopía Confocal , Células PC12 , Pirimidinonas/química , Ratas , ViscosidadRESUMEN
Fe3+ ions play an important role in both biological and environmental field. In this work, two novel rhodamine-based colorimetric and fluorescent probes (RBA2 and RBA3) were designed and synthesized for the efficient detection of Fe3+. Upon the addition of Fe3+, the fluorescence intensity of RBA2 and RBA3 enhanced 108-fold and 222-fold, respectively. RBA2 and RBA3 exhibited a low detection limit which could achieve 12.8â¯nM and 11.0â¯nM. In addition, the binding modes of RBA2 and RBA3 with Fe3+ were proved to be 1:1 stoichiometry in the complexes by Job's plot, ESI-MS and 1H NMR results. The complexing ability of RBA3 with Fe3+ excessed to that of RBA2 that was determined by the binding association constants, and highly consistent with DFT calculations results. Furthermore, RBA2 and RBA3 were further utilized to detect Fe3+ in living cells and real water samples, indicating their promising prospects in biological and environmental field.
Asunto(s)
Teoría Funcional de la Densidad , Colorantes Fluorescentes/química , Hierro/análisis , Rodaminas/química , Espectrometría de Fluorescencia/métodos , Muerte Celular , Células HeLa , Humanos , Concentración de Iones de Hidrógeno , Iones , Límite de Detección , Modelos Moleculares , Espectroscopía de Protones por Resonancia Magnética , Rodaminas/síntesis química , Espectrofotometría Ultravioleta , Agua/químicaRESUMEN
A near-infrared distyryl boron dipyrromethene-based sensor bearing one bis(1,2,3-triazole)amino receptor has been synthesized. This probe selectively and quickly binds to Hg2+ and Cu2+ ions in CH3CN/H2O (5:1â¯v/v) and exhibits remarkably blue-shifted absorption and fluorescence bands due to the inhibition of the intramolecular charge transfer process. The fluorescence changes of this probe upon binding to Hg2+ or Cu2+ ion are totally different, undergoing a ratiometric fluorescence enhancement (for Hg2+) or a fluorescence quenching (for Cu2+) mechanism. The corresponding vivid color changes can be easily seen by the naked eye. This probe was further introduced into Hela cells for living cell imaging and found to discriminate Hg2+ and Cu2+ ions through two near-infrared fluorescence emission channels. These overall results indicate that this Click-derived near-infrared BODIPY-based probe is potentially useful for ratiometric and discriminative detection of Hg2+ and Cu2+ ions in solutions and living cells.
Asunto(s)
Compuestos de Boro/química , Cobre/análisis , Colorantes Fluorescentes/química , Mercurio/análisis , Células HeLa , Humanos , Rayos Infrarrojos , Iones/análisis , Células Tumorales CultivadasRESUMEN
[This corrects the article DOI: 10.1039/C7RA11933J.].
RESUMEN
Two neutral merocyanine-based near-infrared fluorescent probes were for the first time developed through rational engineering of the classical cationic cyanine scaffold IR-780 for in vivo imaging of amyloid-ß plaques. In vivo NIRF imaging revealed that the probe could penetrate the blood-brain barrier and efficiently differentiate the living transgenic and wild-type mice.