FEATURES OF ANTHROPOMETRIC PARAMETERS IN WOMEN OF DIFFERENT MORPHOTYPES WITH POLYCYSTIC OVARY SYNDROME.

The aim - to assess the body size of patients with polycystic ovary syndrome to study their somatotypes and component composition of body weight. To solve the goals and objectives, were prospectively examined 105 women aged 20 to 36 years of different morphotypes. These patients were interviewed according to a specially designed questionnaire and divided into groups: 50 women with multifollicular ovarian structure and 25 - women with polycystic ovary structure. The comparison group consisted of 30 women without disturbances of ovarian structure (healthy women). The body surface area of ​​women with PCOS was significantly larger than in patients of the comparison group and the control group. It was also noted that patients with multifollicular structure of the ovaries had a significant increase in body area from healthy women (p<0,05). The mass-growth rate was significantly higher in patients with polycystic ovary structure compared to patients diagnosed with multifollicular ovarian structure and patients in the control group (p<0.001). It was also noted that in patients with polycystic ovary shoulder girth at rest and in a tense state was greater than in patients with multifollicular structure of the ovaries and, accordingly, the control group (p<0,001, p<0,05). Regarding the examination of chest measurements, no significant changes in diameter, mid-sternum size, lower sternum size, transverse and sagittal measurements were found in patients of the studied groups. However, it was found that in the control group all the above indicators were significantly higher in contrast to patients with polycystic and multifollicular ovarian structure (p<0.001 and p<0.05, respectively). Mateiko's muscle mass was statistically higher in women of the control group in contrast to the groups of women with multifollicular and polycystic ovary structure (p<0.001). We found that Mateiko's bone mass was lowest in patients with multifollicular ovarian structure compared with patients with polycystic ovary structure (p<0.01), while Mateiko's fat mass was higher in patients with polycystic ovary disease (p<0.001). Therefore, we proved that in patients with polycystic ovary structure isolated and predominant mesomorphic component (54.0%; p<0.05), endomesomorphic (9.1%; p<0.05) and indeterminate components 4.5%, p<0.05), and in patients with multifollicular structure of the ovaries is dominated by ectomorphic component (36.7%; p<0.05), ectomesomorphic component (8.2%; p<0.05 ).

Phylogenetic relationships of two Ukrainian tomato isolates of potato virus M and genetic variability analysis of its population.

The aim of this study was to investigate biological and molecular properties of two Ukrainian tomato isolates of potato virus M (PVM), K-16 and Pol-14, to determine their phylogenetic relationships and the genetic variability of PVM isolates. Study of phylogenetic relationships of two Ukrainian tomato PVM isolates with 35 isolates represented in GenBank was conducted. It was found that the coat protein (CP) gene sequence identity between two Ukrainian PVM isolates is 94.3% at the nucleotide level and 100% at the amino acid level. The highest level of the sequence identity (97.0% and 96.5% nt and 100% aa) have the isolates K-16 and Pol-14 with the German potato isolate DSMZ PV0273, Indian potato isolates Del 123, Del 134, Del 147, M 34 and Chinese isolate from pepino GS-6-2 (isolate K-16), which testifies about their common origin. Ukrainian tomato isolates K-16 and Pol-14 belong together with all European, Chinese, Iranian, Indian isolates to PVM-o clade or group I. It was found that the nucleotide substitutions in the capsid protein gene of all tomato PVM isolates (except the Italian) are synonymous. Analysis showed that the global dN/dS ratio for the entire CP gene sequences used in the study was 0.041 (p Keywords: potato virus M; Solanum lycopersicum; phylogenetic analysis; genetic variability; selection pressure.

Building a national hernia registry in South Africa: initial ventral hernia repair results from a diverse healthcare sector.

PURPOSE: The purpose of this paper was to introduce a new registry in a developing country by describing the demographics, management and 30-day outcomes of patients undergoing ventral hernia repair in the public and private healthcare sectors of South Africa. METHODS: This study was a retrospective review of a prospectively maintained hernia registry from the 1st of February 2019 to 29th of February 2020. RESULTS: 353 ventral hernia repair cases were recorded of which 47% were incisional hernias and the remainder were primary hernias. The median age was 54 years with even distribution of males and females. Half of the patients were obese with a median BMI of 31 kg/m2. The private sector performed 190 cases (54%) and the public sector 163 cases (46%). The public sector had more current smokers undergoing elective repairs, 28% vs 15%, p = 0.01 and performed more emergency repair cases, 21% vs 8%, p < 0.01. The majority (89%) of hernias were repaired with mesh and one-third were repaired laparoscopically. 30 day follow up was obtained in 30% of cases, the private sector had better follow up rates (42% vs 14%). CONCLUSION: Participation in the HIG (SA) registry was low with poor follow up over the first year. Ongoing prospective data capture on the HIG (SA) hernia registry will continue to provide further insights into hernia repair practices in South Africa.

[The virus infection progression in potato plants in conditions of simulated microgravitation].

The influence of simulated microgravitation (clinostating) on the proceeding of mixed virus infection in Zhuravushka variety potato plants, infected with potato viruses X and M (PVX, PVM) was investigated. It was shown, that sensitivity of "virus-plant" system to microgravitation depends both on pathogene species and clinostating mode. Vertical clinostating was found to be more effective than horizontal one. By means of biotest, PCR and ELISA it was detected that potato plants, infected with PVX and PVM are released from PVX under the influence of clinostating during 19-47 days. Assume, that microgravitation is able to induce some protective reactions in plants that influence virus reproduction.

[Experience with combined spinal and epidural anesthesia at cesarean section].

The paper analyzes some experience gained in using various modes of regional anesthesia as an anesthetic appliance at cesarean sections and comparatively characterizes various types of central segmental blocks. The results of 213 cases of cesarean section performed under spinal or combined spinal and epidural anesthesia (CSEA) were generalized by the following parameters: block onset, maternal and fetal action, the quality of anesthesia and postoperative analgesia, which leads to the conclusion that CSEA is the method of choice.

[Chromatographic behavior of the hepatitis B virus surface antigen in donor plasma]. / Khromatograficheskoe povedenie poverkhnostnogo antigena virusa gepatita B v donorskoi krovi.

Behavior of HBsAg in adsorption chromatography of the antigen-containing plasma in a column with unmodified macropore silica was studied. Under the employed conditions of overlaying and elution, five maximum yields of plasma proper proteins from the column (peaks 1-5) were observed. HBsAg was determined mainly in the material of peaks 4 and 5 in which the dominating host protein was lipoprotein HDL. No HBsAg was detected in albumin-enriched fraction. Similarities of chromatographic behavior of all the three classes of particles whose surface is presented by HBsAg, 20 nm particles, filamentous forms, and Dane particles, were observed.

[Isolation of glycoproteins of the Venezuelan equine encephalomyelitis virus and an evaluation of their immunogenic activity]. / Vydelenie glikoproteidov virusa venesuél'skogo éntsefalomielita loshadei i otsenka ikh immunogennoi aktivnosti.

Isolation of purified Venezuelan equine encephalomyelitis virus glycoproteins by means of a new Soviet nonionic detergent, MESK, is described. The MESK detergent was shown to permit isolation of approximately 70% of virus particle glycoproteins. The resulting preparation had a high hemagglutinating activity, contained no admixtures of foreign proteins and was not infectious. The study of the immunogenicity of purified glycoproteins in experimental mice and rabbits showed them to be capable of inducing high levels of serum antibodies. The immunogenicity of the isolated glycoproteins was comparable to their immunogenicity as components of virus particles. Treatment of the virus with MESK detergent also yielded preparations with predominant content of capsid protein. The described procedure for disintegration and purification of viral proteins is technologically simple and may be the basis for manufacture of a subunit vaccine. The resulting material may also be used for preparation of diagnosticums and in laboratory studies.

[Binding of the surface antigen of the hepatitis B virus with antibodies studied with different denaturation exposures and with different analytical methods]. / Izuchenie sviazyvaniia poverkhnostnogo antigena virusa gepatita B s antitelami pri razlichnykh denaturiruiushchikh vozdeistviiakh i raznykh metodakh analiza.

The effect of delipidizing agents and a reducing agent on the antigenicity of 20 nm particles of hepatitis B virus surface antigen (HBsAg) was studied. The antigenicity was determined from the capacity of binding with antibody in passive hemagglutination test (PHAT) and dot-blot immune binding (DBIB). Delipidation was found to lead to an apparent decrease of antigenicity caused by aggregation. Subsequent destruction of the aggregates by treatment with sodium dodecylsulphate (SDS) reduced the antigenicity. Treatment with the reducing agent in the presence of SDS results in the loss of antigenicity detectable by PHAT, the DBIB titres remaining unchanged. Some examples demonstrate a high sensitivity of DBIB in detection of HBsAg. The advantages of the latter test over other methods of immune diagnosis are discussed.