Tuber wenchuanense, a holarctic truffle with a wide range of host plants and description of its ectomycorrhiza with spruce.

Tuber wenchuanense ascomata (Ascomycota, Pezizales), a species originally described from Sichuan (China), were found in the Tatra Mountains in southern Poland. The purpose of this work was to (i) report and assess the first case of the holarctic natural distribution of a Tuber species, (ii) amend the original description of the species, (iii) summarize data on its host plants and (iv) describe its ectomycorrhiza. Specimens of Tuber wenchuanense from the Tatra Mountains were studied morphologically and molecularly. The ectomycorrhiza of this truffle with Picea abies was described for the first time. The distribution of T. wenchuanense, which is reconstructed based on sequences deposited in the publicly available nucleotide sequence databases, makes it the first holarctic Tuber species and the one with the northernmost habitat. In fact, its habitat is confined mainly to mountain coniferous forests and alpine and arctic tundra; although, according to known observations, the fruiting bodies of T. wenchuanense can be produced only under conifers. Based on the sequences of the internal transcribed spacer, this species appears to have low genetic variability over the entire distribution range. The phylogenetic tree showed that some of the unidentified phylotypes from the Rufum clade found by other researchers belong to T. wenchuanense. The ecological implications of these findings are discussed.

Next-Generation Sequencing for Evaluating the Soil Nematode Diversity and Its Role in Composting Processes.

Biodiversity within composting systems involves a variety of microorganisms including nematodes. In the research, nematode populations were monitored within six simultaneously operating composting processes. These processes involved varying proportions of feedstock materials. The primary objective was to evaluate the consistency of nematode community succession patterns across the composting processes over a time of 3 months. During the study, samples were taken every month to isolate nematodes, determine the population density of the five trophic groups (per genus) and determine the dominant nematode species. It was shown that the bacterial-feeding community maintained dominance, while the fungus-feeding nematodes gradually increased in dominance as the maturation process progressed. The presence of predatory nematodes Mononchoides which were initially absent, along with the total absence of parasitic nematodes in the late stages of waste stabilization, serves as strong evidence for the reliable evaluation of the biodegradable waste processing level. Based on the obtained results, it is evident that the succession of nematode communities holds promise as a reliable method for evaluating compost maturity.

First molecular identification of Suillia gigantea (Meigen, 1830) (Diptera: Heleomyzidae).

In 2017, the presence of the fly Suillia gigantea (Meigen, 1830) was noted in Poland, after many years of research related to the ecology of insects associated with the fruiting bodies of hypogeous fungi. Finally, in 2020, after further studies, the distribution of the truffle fly in Poland was confirmed. Six adults were reared from larvae inhabiting the fruiting bodies of Burgundy truffle (Tuber aestivum Vittad. (1831)). The fungi were harvested in southern Poland. Morphological and genetic analyses of the insect specimens were performed. For the first time, the DNA sequence of this fly species was isolated. This is the first record of S. gigantea in Poland, although it has already been reported from neighbouring countries. The development of S. gigantea, also known as the truffle fly, is associated with hypogeous fungi, mainly belonging to the genus Tuber P. Micheli ex F.H. Wigg. (1780). The diptera larvae develop inside the fruiting bodies. This type of damage causes great losses in truffle production. Containment of these losses is of great interest to the truffle industry. Knowledge of truffle-inhabiting insects is crucial for the development of effective methods to protect truffle crops in Europe.

Hybridization hotspots at bat swarming sites.

During late summer and early autumn in temperate zones of the Northern Hemisphere, thousands of bats gather at caves, mainly for the purpose of mating. We demonstrated that this swarming behavior most probably leads not only to breeding among bats of the same species but also interbreeding between different species. Using 14 nuclear microsatellites and three different methods (the Bayesian assignment approaches of STRUCTURE and NEWHYBRIDS and a principal coordinate analysis of pairwise genetic distances), we analyzed 375 individuals belonging to three species of whiskered bats (genus Myotis) at swarming sites across their sympatric range in southern Poland. The overall hybridization rate varied from 3.2 to 7.2%. At the species level, depending on the method used, these values ranged from 2.1-4.6% in M. mystacinus and 3.0-3.7% in M. brandtii to 6.5-30.4% in M. alcathoe. Hybrids occurred in about half of the caves we studied. In all three species, the sex ratio of hybrids was biased towards males but the observed differences did not differ statistically from those noted at the population level. In our opinion, factors leading to the formation of these admixed individuals and their relatively high frequency are: i) swarming behaviour at swarming sites, where high numbers of bats belonging to several species meet; ii) male-biased sex ratio during the swarming period; iii) the fact that all these bats are generally polygynous. The highly different population sizes of different species at swarming sites may also play some role. Swarming sites may represent unique hybrid hotspots, which, as there are at least 2,000 caves in the Polish Carpathians alone, may occur on a massive scale not previously observed for any group of mammal species in the wild. Evidently, these sites should be treated as focal points for the conservation of biodiversity and evolutionary processes.