Online extraction and enrichment coupling with high-speed counter-current chromatography for effective and target isolation of antitumor anthraquinones from seeds of Cassia obtusifolia.

Traditional bioassay-guided investigation of bioactive compounds from natural products comprises critical steps, such as extraction, repeated column separation, and activity assay. Thus, the development of facile, rapid, and efficient technology is critically important. Here, a HepG2 cell-based extraction method was first developed to rapidly screen potential antitumor compounds from the seeds ofCassia obtusifolia. Then, an online extraction and enrichment-high-speed counter-current chromatography (HSCCC) strategy was fabricated to facilely and efficiently isolate target antitumor compounds, which included direct extraction from solid C. obtusifolia, removal of polar interferences, enrichment of target compounds, and preparative isolation by HSCCC using flow rate stepwise increasing mode. After further purification by Sephadex LH-20 column, five antitumor anthraquinones, aurantio-obtusin, 1-desmethylaurantio-obtusin, chryso-obtusin, obtusin, and questin, were obtained for structural characterization and bioassay verification. The results may not only provide new perspectives for facile and rapid investigation of bioactive compounds from complex natural products, but also offer a scientific basis for the potential applications of C. obtusifolia.

Rapid profiling of potential antitumor polymethoxylated flavonoids in natural products by integrating cell biospecific extraction with neutral loss/diagnostic ion filtering-based high-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry.

INTRODUCTION: Citri Reticulatae Pericarpium Viride (CRPV, Qing Pi in Chinese) has been widely used in traditional Chinese medicine. Polymethoxylated flavonoids (PMFs), which are a special group of flavonoids with strong antitumor activity, are broadly distributed in citrus peels. However, systematic investigation of antitumor PMFs in CRPV has received little attention to date. OBJECTIVES: An MCF-7 cell biospecific extraction method integrated with neutral loss/diagnostic ion filtering-based HPLC-QTOF-MS/MS strategy was developed for rapid and specific profiling of antitumor PMFs and systematic identification of PMFs in CRPV. METHODOLOGY: By incubating MCF-7 cells with CRPV extract, potential antitumor PMFs specifically bound to cells and were isolated. Then, by systematic investigation of fragmentation pathways, neutral loss and diagnostic ion filtering strategies were proposed to comprehensively and accurately identify PMFs. RESULTS: Sixteen antitumor PMFs were unambiguously or tentatively identified. Among them, minor compound 15 (5-hydroxy-6,7,8,3',4'-pentamethoxyflavone with a free hydroxyl group at C-5) exhibited excellent antitumor activity, with an IC50 value of 2.81 ± 0.76 µg/mL, which is lower than that of 5-fluorouracil (IC50 , 4.92 ± 0.83 µg/mL). Nobiletin (12) and tangeretin (16), two major PMFs, presented moderate antitumor activities with IC50 values of 13.06 ± 1.85 and 17.07 ± 1.18 µg/mL, respectively, and their contents were sensitively and precisely determined. CONCLUSIONS: To the best of our knowledge, this is the first report on the systematic investigation of antitumor PMFs in CRPV. The study will lay a foundation for the quality control and clinical application of CRPV.

Diagnostic ion filtering targeted screening and isolation of anti-inflammatory iridoid glycosides from Hedyotis diffusa.

Efficient and targeted screening and isolation of bioactive compounds from complex natural products is still a challenging work. Herein, diagnostic ion filtering based high-performance liquid chromatography-quadrupole time-of-flight-tandem mass spectrometry was firstly developed to screen six main iridoid glycosides from Hedyotis diffusa. Then, online extraction-high-speed counter current chromatography was proposed for targeted enrichment and preparative isolation using ethyl acetate/n-butanol/water (4.5:0.5:5, v/v/v) as solvent system. After that, Sephadex LH-20 column chromatography using methanol as solvent system was selected for further purification of six iridoid glycosides with purities over 98%. They were finally identified as monotropein, desacetylasperuloside acid, asperuloside, 6-O-(Z)-p-coumaroyl scandoside methyl ester, 6-O-(Z)-feruloyl scandoside methyl ester, and 6-O-(E)-p-coumaroyl scandoside methyl ester. And their anti-inflammatory activities were evaluated and confirmed by lipopolysaccharide activated RAW 264.7 macrophages. Obviously, the results provide a scientific basis for the potential applications of H. diffusa, and the developed methodology is efficient and reliable for targeted screening and isolation of bioactive compounds from natural products.

Flavonoid aglycone-oriented data-mining in high-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry: efficient and targeted profiling of flavonoids in Scutellaria barbata.

The high-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (HPLC-QTOF-MS/MS) technique is a powerful tool for compound identification in complex natural products. However, untargeted MS/MS data analysis needs skillful experience and sometimes neglects minor compounds, which are co-eluted with major ones or overshadowed by the matrix. Flavonoids are the main bioactive components in Scutellaria barbata, and the total flavonoid content is 47.02 ± 3.23 mg QE/g DW. Although some flavonoid aglycones and their O-glycosides have been found in S. barbata, comprehensive profiling of flavonoids is unknown. Therefore, we report a flavonoid aglycone-oriented data-mining strategy for efficient and targeted profiling of flavonoids in S. barbata. The strategy includes four steps: (1) HPLC-QTOF-MS analysis of S. barbata; (2) construction of a flavonoid aglycone-based database according to biosynthetic pathway analysis and reported data; (3) extraction of through flavonoid aglycone-based ion chromatography; (4) identification of targeted flavonoids by MS/MS analysis. As a result, 45 flavonoids, including 24 flavones, 1 flavonol, 13 flavanones, and 7 flavanonols, were unambiguously or tentatively identified, while 20 of them were reported in S. barbata for the first time. Moreover, 14 available flavonoids were sensitively, precisely, and accurately determined by standard calibration curves, with limit of detection at 0.06 to 1.55 µg/g, limit of quantification at 0.16 to 3.70 µg/g, relative standard deviation (RSD) less than 9.0% for intra- and inter-day variations, and recovery at 92.6-108.1%. The matrix did not obviously suppress or enhance the ionization of 14 flavonoids, and finally their contents ranging from 0.04 to 4.49 mg/g in S. barbata were successfully achieved. Collectively, our results demonstrate that an efficient, reliable, and valuable strategy has been provided to rapidly and sensitively screen, profile, and quantify chemical components of complex natural products. Graphical abstract.

Correction to: One-pot fabrication of dual-emission and single-emission biomass carbon dots for Cu2+ and tetracycline sensing and multicolor cellular imaging.

The authors would like to call the reader's attention to the fact that unfortunately the wrong file was published as Fig. 2.

One-pot fabrication of dual-emission and single-emission biomass carbon dots for Cu2+ and tetracycline sensing and multicolor cellular imaging.

Dual-emission and single-emission carbon dots (DCDs and SCDs) have been simultaneously synthesized by one-pot solvothermal treatment of leek. Different graphitization and surface functionalization were responsible for their distinction in fluorescence characteristics. DCDs with an average size of 5.6 nm exhibited two emissions at 489 and 676 nm under 420-nm excitation. Complexation between DCDs' surface porphyrins and Cu2+ led to quenching of the 676-nm emission, which resulted in the ratiometric determination of Cu2+ with a limit of detection (LOD) of 0.085 µM. SCDs, containing additional sulfur element (0.50%) with an average size of 7.7 nm, presented a single emission at 440 nm under 365-nm excitation. The static quenching and inner filter effects between SCDs and tetracyclines (TCs) made SCDs a fluorescence nanoprobe for TCs' determination with LODs of 0.26-0.48 µM. Applications of DCDs and SCDs for respective determination of Cu2+ and TCs in milk and pig liver samples were successfully demonstrated. Moreover, good photostability, low toxicity, and outstanding biocompatibility made DCDs and SCDs suitable for multicolor cellular imaging. Results indicate that natural products are excellent raw materials to controllably synthesize CDs with prominent physicochemical and fluorescence properties.Graphical abstract.

Homoisoflavonoids profiling of Ophiopogon japonicus by off-line coupling high-speed countercurrent chromatography with high-performance liquid chromatography-diode array detector-quadrupole time-of-flight tandem mass spectrometry.

Roots of Ophiopogon japonicus have been used as a functional food ingredient and traditional Chinese medicine for a long time in China. Homoisoflavonoids are one of the major kinds of bioactive compounds in O. japonicus; however, literature data about its homoisoflavonoids profile are scarce because of the complex ingredients with low abundance. Here, homoisoflavonoid fraction was prepared by petroleum ether extraction. Then, a high-speed countercurrent chromatography off-line coupling with high-performance liquid chromatography-diode array detector-quadrupole time-of-flight tandem mass spectrometry was developed for systematic identification of homoisoflavonoids. After that, 39 homoisoflavonoids, including 29 homoisoflavanone and 10 homoisoflavone, were unambiguously or tentatively identified, while 12 of them were reported in O. japonicus for the first time. Finally, eight available homoisoflavonoids were sensitively, precisely, and accurately determined by standard calibration curves, with limit of detection and limit of quantification in the range of 0.05-0.30 µg/mL and 0.12-0.66 µg/mL, relative standard deviation less than 7.3% for intra- and interday variations, and recovery at 94.5-105.2%. Collectively, our developed method is efficient, reliable, and valuable to profile chemical components of complex natural products.

Dual-emissive carbon dots for dual-channel ratiometric fluorometric determination of pH and mercury ion and intracellular imaging.

Dual-emissive carbon dots (CDs) were fabricated for dual-channel ratiometric fluorometric determination of pH and mercury ion (Hg2+) and intracellular imaging. Dual-emissive CDs were synthesized by one-pot solvothermal treatment of cabbage. The CDs exhibited two distinctive fluorescence emissions at 500 and 678 nm under single excitation at 410 nm. The green emission (500 nm) had reversible linear response to pH (7.0-12.0) due to deprotonation and protonation of surface functional groups and their non-covalent interactions. On the other hand, the red emission (678 nm) had efficient and selective fluorescence response to Hg2+ by formation of non-emission complex between CDs and Hg2+. The limit of detection (LOD) and limit of quantification (LOQ) for Hg2+ were 6.25 and 20.63 nM, respectively. The CDs have been successfully applied for label-free ratiometric fluorometric determination of pH and Hg2+ in fish and human serum samples with good recoveries (92.0-108.3%). In addition, the CDs had excellent photostability, low cytotoxicity, and good biocompatibility for intracellular imaging. All in all, the system was multi-functional in determination, high in sensitivity, and excellent in selectivity, which demonstrated wide and promising applicability for biosensing and bioimaging in the future. Graphical abstract Schematic presentation of dual-emission carbon dots (CDs) synthesized by solvothermal treatment of cabbage for dual-channel determination of pH and Hg2+.

Online extraction and cleanup-quadrupole time-of-flight tandem mass spectrometry for rapid analysis of bioactive components in natural products.

The challenges in direct analysis of a complex system (e.g., natural product, food, biological samples) by mass spectrometry (MS) are the sophisticated sample preparation methods and ionization suppression by matrix interferences. Consequently, a novel online extraction and cleanup-quadrupole time-of-flight tandem mass spectrometry (OLEC-QTOF-MS/MS) system was developed for rapid, efficient, and sensitive analysis of flavonoids in Citri Reticulatae Pericarpium (CRP). For the OLEC strategy, a guard column packed with solid CRP (0.5 mg) and C18 gel was positioned on a manual injection valve, in which interferences with large polarities were online removed by methanol-0.1% formic acid (25:75, v/v) for 3 min, while target flavonoids were online extracted by methanol-0.1% formic acid (70:30, v/v) for 10 min for the subsequent QTOF-MS/MS analysis. The method was validated using official marker, hesperidin, by external standard method. Excellent linear ranges from 0.02 to 52.0 µg L-1 (R2, 0.9935) with a limit of detection (LOD) of 0.006 µg were obtained. Acceptable reproducibility (RSD 8.1 and 9.6% for intra- and inter-day variations) and recoveries (from 99.5 to 112.0%) were also attained. In addition, 20 flavonoids in CRP were identified according to their exact mass and fragmentation ions in MS/MS spectra, and five of them were reported for the first time. Obviously, OLEC-QTOF-MS/MS presented several advantages, such as simple operation and high sensitivity, which provided new perspectives for rapid analysis of bioactive components in complex natural products. Graphical Abstract ᅟ.

Cetyltrimethyl ammonium mediated enhancement of the red emission of carbon dots and an advanced method for fluorometric determination of iron(III).

Red-emissive carbon dots (CDs) were synthesized by one-step hydrothermal technique using citric acid (CA), and urea in N,N-dimethylformamide (DMF) solution. The CDs has an average diameter of 2.3 nm, excitation/emission maxima at 553/606 nm, and a low photoluminescence quantum yield (4%). Fluorescence is weakly quenched by the ions Fe3+, Hg2+, Cu2+, Co2+, Zn2+, Ca2+, Ni2+, and Pb2+. After addition of cetyltrimethyl ammonium ion (CTAB), electrostatic interaction between negatively charged CDs and CTAB causes the CDs to self-aggregate. The formation of CD/CTAB increases the average particle diameter to around 13 nm and enhances the quantum yield to 24%. The hydrophobic segments of CTAB twined into a network structure can selectively trap Fe3+ and then interact with surface groups of the CDs to cause quenching. The CD/CTAB nanoprobe enables fluorometric determination of Fe3+ with a linear response in the 0.10-10 µM concentration range and a 0.03 µM limit of detection. The probe was utilized for determination of Fe3+ in human serum samples, and satisfactory results were obtained. Graphical abstractSchematic representation of fluorometric analysis of Fe(III) ion by cetyltrimethyl ammonium ion (CTAB) mediated red emission carbon dots (CDs). The hydrophobic segments of CTAB twined into a network structure can selectively trap Fe(III) and then interact with surface groups of the CDs to cause quenching.

Direct from solid natural products to pure compounds in a single step: Coupling online extraction with high-speed counter-current chromatography.

Extraction is the most important step in the purification of bioactive compounds from natural products. This study introduces a simple online extraction strategy coupled with high-speed counter-current chromatography for efficient extraction and purification of bioactive components from solid natural products. For online extraction strategy, 1.0 g of ground Mangnolia officinalis or Piper nigrum was loaded into a guard column, which was then positioned on the manual injection valve instead of the sample loop. Bioactive components were directly extracted by the mobile phase of high-speed counter-current chromatography, and then transferred into high-speed counter-current chromatography for purification. In addition, the compatibility of the developed methodology for direct purification of bioactive components from fresh M. officinalis was successfully demonstrated. Obviously, in comparison with traditional offline heat-reflux extraction, online extraction avoided the instrument, time, solvent, and energy consumption, and purified two phenolic compounds (honokiol and magnolol) from M. officinalis and three alkaloids (piperyline, piperine, and piperanine) from P. nigrum with high extraction efficiency. The superiority of the developed methodology is to establish an easy, rapid, and efficient technique for the purification of a wide variety of bioactive components from solid natural products.

Hinge-like paper-based dual-channel enzyme-free ratiometric fluorescent microfluidic platform for simultaneous visual detection of carbaryl and glyphosate.

On-site multi-pesticide residues detection is particularly urgent and challenging. Here, we fabricated an enzyme-free ratiometric fluorescent detection system in combination with a hinge-like dual-channel 3D microfluidic paper analytical device (3D µPAD) for simultaneous visual detection of carbaryl and glyphosate. Blue-emission 1-naphthol (Em. 470 nm) was hydrolyzed from carbaryl, while yellow-emission 2,3-diaminophenazine (Em. 570 nm) was produced with the aid of Cu2+ for glyphosate sensing. Inner-filter effect between 1-naphthol or 2,3-diaminophenazine and green-emission carbon dots (Em. 510 nm) realized two ratiometric fluorescent detection systems. Remarkable color variation of green-blue for carbaryl (50.00-1100 µΜ) and yellow-green for glyphosate (5.00-600 µΜ) were observed on a dual-channel 3D µPAD without crosstalk. Their detection limits were 1.11 and 0.63 µΜ, respectively. The strategy realized simultaneous visual detection of carbaryl and glyphosate in food/herbal with excellent accuracy (spiked recoveries, 91.00-107.2%), high precision (RSD ≤ 8.43%), and superior selectivity.

Design of a sustainable light-up flavonol probe for dual-ratiometric fluorescent sensing and visual differentiating ammonia and hydrazine.

Ammonia (NH3) and hydrazine (N2H4) present potential risks to human health, food and environmental safety. A sustainable flavonol-based probe, quercetin pentaacetate (QPA, weak blue emission 417 nm), was fabricated for dual-ratiometric fluorescent sensing and visual differentiating NH3 and N2H4. Excited state intramolecular proton transfer-on products with green (487 nm) and yellow (543 nm) emissions occurred as meeting with NH3 and N2H4, respectively, for their different nucleophilicities. Such a promising response offered a great opportunity of QPA to discriminatively detect NH3 and N2H4 with large Stokes shifts (>122 nm), high sensitivity (limit of detection: 35.4 µM and 0.70 ppm for NH3 solution and gas; 0.26 µM for N2H4 solution), excellent accuracy (spiked recoveries from 98.6 % to 105 %), and superior selectivity. Importantly, QPA was utilized for monitoring NH3 vapor in fish spoilage procedures and detecting N2H4 in water samples for food and environmental safety evaluation.

Threaded 3D microfluidic paper analytical device-based ratiometric fluorescent sensor for background-free and visual detection of organophosphorus pesticides.

With the increasing concerns of food safety and environmental protection, it is desirable to develop reliable, effective, and portable sensors for detection of organophosphorus pesticides (OPs). Here, a cascade reaction system integrated with threaded 3D microfluidic paper analytical device (3D µPAD) was firstly developed for background-free and visual detection of OPs in agricultural samples. Butyrylcholinesterase (BChE) hydrolyzed acetylcholine into thiocholine (TCh), which reduced MnO2 nanosheets into Mn2+. With addition of OPs, BChE activity was irreversibly inhibited, and the generation of TCh and the reduction of MnO2 nanosheets were prevented. Then the remaining MnO2 nanosheets oxidized o-phenylenediamine into 2,3-diaminophenazine with yellow-emission fluorescence, which quenched the fluorescence intensity of red-emission carbon dots (RCDs) via inner-filter effect. Based on above mechanism, a ratiometric fluorescent system was established for OPs detection. Threaded 3D µPAD consisted of 4 layers, which allowed to load and/or add reagents to trigger the cascade reaction system for OPs detection. The fluorescent images presented distinguishable color variations from red to yellow with dichlorvos concentrations ranging from 2.5 to 120 µg L-1, and the limit of detection was 1.0 µg L-1. In the practical samples testing, threaded 3D µPAD can eliminate background influence on fluorescent signal for OPs detection. Threaded 3D µPAD integrated with ratiometric sensing platform has merits of accuracy response, facile operation, and background-free detection, which supplies a new alternative approach for on-site pesticide detection.

3D origami paper-based ratiometric fluorescent microfluidic device for visual point-of-care detection of alkaline phosphatase and butyrylcholinesterase.

On-site multiplex enzyme detection is crucial for diagnosis, therapeutics and prognostic. To date, it is still a daunting challenge to develop portable, low-cost, and efficient multi-enzyme detection methods. Herein, a novel sample-in-result-out platform integrating ratiometric fluorescent assays with 3D origami microfluidic paper-based device (µPAD) was developed for simultaneous visual point-of-care testing (POCT) of alkaline phosphatase (ALP) and butyrylcholinesterase (BChE). Cascade catalytic reaction with the same two fluorescent signal indicators was rationally designed to ratiometric fluorescent detection of ALP and BChE: substrate of ALP (pyrophosphate) and product of BChE (thiocholine) can strongly complex with Cu2+, Cu2+ oxidizes o-phenylenediamine to fluorescent 2,3-diaminophenazine (oxOPD) (emission, 565 nm), oxOPD quenches the fluorescence of carbon dots (CDs, emission at 445 nm) via inner filter effect, thus oxOPD/CDs values are relevant to ALP and BChE activities. Then 3D origami µPAD composing of four layers and two parallel channels was fabricated and simply prepared by one-step plotting with black oil-based marker and specific metal molds. After simple folding and unfolding neighboring layers to sequentially initiate reactions of pre-loaded reagents, fluorescent images on the detection zone can be captured by smartphone and analyzed by red-green-blue software for quantitative analysis. Under optimal conditions, the proposed platform was successfully performed to detect ALP and BChE with activity difference at 3 orders of magnitude in human serum samples without any pretreatment procedures. Excellent selectivity, good precision, favorable linear range, and high accuracy were exhibited. Importantly, the platform opens a promising horizon for high-throughput POCT of multiplex biomarkers.

Weaving microscale wool ball-like hollow covalent organic polymers from nanorods for efficient adsorption and sensing.

Microscale covalent organic polymers with a unique 3D hollow wool ball-like morphology have been woven from 1D nanorods by a cascade emulsion strategy with a large surface area (284 m2 g-1), which showed great potential for simultaneous removal (Qmax, 358.15 mg g-1) and fluorescent detection (detection limit, 8.0 µg L-1) of bisphenol A.

Highly specific esterase activated AIE plus ESIPT probe for sensitive ratiometric detection of carbaryl.

Fabrication of facile, sensitive, and accurate pesticide detection strategies plays crucial roles in food safety, environmental protection, and human health. Here, a novel esterase activatable aggregation-induced emission (AIE) plus excited-state intramolecular proton transfer (ESIPT) probe, kaempferol tetraacetate, was designed and synthesized from purified natural kaempferol for ratiometric sensing of carbaryl. Acetate groups are introduced as the esterase reactive sites and AIE plus ESIPT initiator. Kaempferol tetraacetate is an aggregation-caused quenching compound that shows fluorescent (FL) emission at 415 nm. Esterase specifically hydrolyzes kaempferol tetraacetate to kaempferol with AIE plus ESIPT characteristics (distinct FL emission, 530 nm; a large Stokes shift, 165 nm within a short time (8 min). Molecular docking and kinetics performance indicate the high affinity and specific hydrolysis of esterase and kaempferol tetraacetate. Carbaryl inhibits the activity of esterase to efficiently suppress the production of kaempferol. Thus, a facile ratiometric assay strategy is constructed for carbaryl detection. By measuring the FL intensity ratio, the proposed strategy presents high selectivity and reliability with a wide linear range from 0.02 to 2.00 µg L-1 and a very low limit of detection at 0.007 µg L-1. Furthermore, appropriate recovery from 93.75% to 108.67% with a relative standard deviation less than 5.66% for real sample analysis indicates good accuracy and precision. All results indicate that the fabricated strategy offers a new way for facile, sensitive, and accurate detection of carbaryl in real complex samples.

Simultaneous sensing γ-glutamyl transpeptidase and alkaline phosphatase by robust dual-emission carbon dots.

Rapid, convenient, sensitive and simultaneous detection of distinct enzymes is urgently needed for diagnosis, therapeutics and prognostic of related diseases. Here, a new strategy for simultaneous monitoring γ-glutamyl transpeptidase (GGT) and alkaline phosphatase (ALP) activity has been fabricated based on dual-emission carbon dots (CDs). CDs were prepared by solvothermal treatment of Actinidia chinensis, which presents two fluorescent emissions at 471 nm (blue channel) and 671 nm (red channel). GGT and ALP activity can be detected based on inner filter effect (IFE) and static quenching effect (SQE) of blue and red channels of CDs, respectively. Linear ranges were 2.5-90 U L-1 and 5-200 U L-1, and limit of detection (LOD) were 0.71 U L-1 and 1.2 U L-1 for GGT and ALP, respectively. Developed CDs can monitor GGT and ALP activity in human serum samples with satisfied recoveries (99.3%-108.6% for GGT, 98.4%-105.4% for ALP). Furthermore, the combination of CDs to sense GGT and ALP activity with OR logic gate can predict human health status. The design and application of dual-emission CDs can also be extended as promising tools to detect multianalytes using different channel signals.

Profiling of tyrosinase inhibitors in mango leaves for a sustainable agro-industry.

Although mango leaves are the main ingredients in some traditional Chinese medicine preparations and folk tea, they with considerable quantities are usually discarded as agricultural waste. Thus, to extend their potential, reverse ultrafiltration-HPLC-DAD-QTOF-MS/MS combining with key ion filtering strategy was proposed to efficiently fish and systematically identify tyrosinase inhibitors in ethyl acetate fraction of mango leaves, which has the highest total phenolic content (40.00 ± 0.84 mg GAE/g DW) and tyrosinase inhibition activity (IC50, 17.62 ± 1.26 µg/mL). Finally, 36 polyphenolic tyrosinase inhibitors were unambiguously characterized or tentatively identified, and three of them were found in mango leaves for the first time. Results suggested that the proposed strategy was powerful for effective identification of bioactive compounds in complex mixtures (e.g. food, agricultural and sideline products), and the findings would lay a foundation for potential applications of mango leaves in pharmaceutical, cosmetic, and food industrial fields.

White pepper-derived ratiometric carbon dots for highly selective detection and imaging of coenzyme A.

A new-style white pepper derived dual-emission carbon dots (CDs) with a quantum yield of 10.4% was designed and facile constructed with one-pot solvothermal method. The green emission (520 nm) had an efficient and special "turn-on" fluorescence sensing of coenzyme A (CoA) with the aid of Cu2+, while red emission (668 nm) barely changed and worked as reference. In the concentration range (0-150 µM), relative fluorescence intensity ratios (F520/F668) showed excellent linear correlation with concentrations of CoA, and detection limit was as low as 8.75 nm. Moreover, the strategy has been successfully applied for label-free detection of CoA in real pig liver samples with good recoveries (93.3-108.0%). Notably, the synthesized CDs had durable fluorescence, low cytotoxicity, and good biocompatibility for cellular imaging, which demonstrated wide and promising applicability for biosensing and bioimaging in the future.