Ultrahigh-Resolution X-ray Tomography.

Ultrahigh-resolution three-dimensional images of a microscopic test object were made with soft x-rays collected with a scanning transmission x-ray microscope. The test object consisted of two different patterns of gold bars on silicon nitride windows that were separated by approximately 5 micrometers. Depth resolution comparable to the transverse resolution was achieved by recording nine two-dimensional images of the object at angles between -50 and +55 degrees with respect to the beam axis. The projections were then combined tomographically to form a three-dimensional image by means of an algorithm using an algebraic reconstruction technique. A transverse resolution of approximately 1000 angstroms was observed. Artifacts in the reconstruction limited the overall depth resolution to approximately 6000 angstroms; however, some features were clearly reconstructed with a depth resolution of approximately 1000 angstroms.

Demonstration of X-ray Holography with an X-ray Laser.

An x-ray hologram was made by means of an x-ray laser and a laser-quality near normal incidence x-ray mirror. The high brightness and large coherence lengths of x-ray lasers now offer the potential for in vitro three-dimensional high-resolution holographic images of dynamically varying biological microstructures.

Measurement of Laser-Plasma Electron Density with a Soft X-ray Laser Deflectometer.

A soft x-ray laser (wavelength lambda = 15.5 nanometers) was used to create a moiré deflectogram of a high-density, laser-produced plasma. The use of deflectometry at this short wavelength permits measurement of the density spatial profile in a long-scalelength (3 millimeters), high-density plasma. A peak density of 3.2 x 10(21) per cubic centimeter was recorded.

X-ray laser microscopy of rat sperm nuclei.

The development of high brightness and short pulse width (< 200 picoseconds) x-ray lasers now offers biologists the possibility of high-resolution imaging of specimens in an aqueous environment without the blurring effects associated with natural motions and chemical erosion. As a step toward developing the capabilities of this type of x-ray microscopy, a tantalum x-ray laser at 44.83 angstrom wavelength was used together with an x-ray zone plate lens to image both unlabeled and selectively gold-labeled dried rat sperm nuclei. The observed images show approximately 500 angstrom features, illustrate the importance of x-ray microscopy in determining chemical composition, and provide information about the uniformity of sperm chromatin organization and the extent of sperm chromatin hydration.

Wavelength choice for soft x-ray laser holography of biological samples.

The choice of an optimal wavelength for soft x-ray holography is discussed, based on a description of scattering by biological structures within an aqueous environment. We conclude that wavelengths slightly longer than the 43.7-A carbon K-edge provide a good trade off between minimizing the necessary source power and the dose absorbed by the sample and maximizing the penetrability of the x-rays through wet samples. This differs from the previous notion that wavelengths within the water window (between 23.2 A and 43.7 A) would be the best for holography. The problem of motion resulting from the absorption of x rays during a short exposure is described. The possibility of using ultrashort exposures in order to capture the image before motion can compromise the resolution is explored. The impact of these calculations on the question of the feasibility of using an x-ray laser for holography of biological structures is discussed.

Wavelength choice for soft x-ray laser holography of biological samples: errata.

This Letter points out several errors made in the printing of the original submission.

Time-resolved measurement of double-pass amplification of soft x rays.

We report the first time-resolved measurements of emission from a double-pass soft x-ray laser cavity. In these experiments the output signal from a selenium x-ray laser had two temporal components clearly identifiable as the single- and double-pass emission, with the double-pass amplified signal more intense than the single pass. In addition to an unequivocal demonstration of double-pass amplification of soft x rays, the data provide information about of the time-dependent gain in these x-ray laser media, suggesting an effective gain-length profile which rises more slowly and falls-off more rapidly than predicted by state of the art hydrodynamics and kinetics codes.

Extreme-ultraviolet interferometry at 15.5 nm using multilayer optics.

The development of multilayer mirror technology capable of operating in the range of 3-30 nm and the construction of thin membranes with excellent uniformity and strength have made it possible to design and implement a Mach-Zehnder interferometer operating at 15.5 nm. We have tested this interferometer by using a soft x-ray laser as a source, and we show its use in probing high-density plasmas.

Demonstration of x-ray microscopy with an x-ray laser operating near the carbon K edge.

High-brightness and short-pulse-width ( approximately 200 ps) x-ray lasers offer biologists the possibility of high-resolution three-dimensional imaging of specimens in an aqueous environment without the blurring effects associated with natural motions. As a first step toward developing the capabilities of this type of x-ray microscopy we have used a tantalum x-ray laser (lambda = 4.483 nm) together with an x-ray zone plate lens to image a test pattern. The observed image shows a detector-limited resolution of approximately 75 nm and paves the way to three dimensional biological imaging with high spatial resolution (20-30 nm).