Microbial bioprocess performance in nanoparticle-mediated composting.

Microbial composting is one of the most cost-effective techniques for degradation, remediation, nutrition, etc. Currently, there is faster growth and development in nanotechnology in different sectors. This development leads nanoparticles (NPs) to enter into the composts in different ways. First, unintentional entry of NPs into the composts via: waste discharge, buried solid waste, surface runoff, direct disposal into wastes (consumer goods, food, pharmaceuticals, and personal care products). Second, intentional mediation of the NPs in the composting process is a novel approach developed to enhance the degradation rate of wastes and as a nutrient for plants. The presence of NPs in the composts can cause nanotoxicity. Conversely, their presence might also be beneficial, such as soil reclamations, degradation, etc. Alternatively, metal NPs are also helpful for all living organisms, including microorganisms, in various biological processes, such as DNA replication, precursor biosynthesis, respiration, oxidative stress responses, and transcription. NPs show exemplary performance in multiple fields, whereas their role in composting process is worth studying. Consequently, this article aids the understanding of the role of NPs in the composting process and how far their presence can be beneficial. This article reviews the significance of NPs in: the composting process, microbial bioprocess performance during nano composting, basic life cycle assessment (LCA) of NP-mediated composting, and mode of action of the NPs in the soil matrix. This article also sheds insight on the notion of nanozymes and highlights their biocatalytic characterization, which will be helpful in future composting research.

Drug induced liver injury - a 2023 update.

Drug-Induced Liver Injury (DILI) constitutes hepatic damage attributed to drug exposure. DILI may be categorized as hepatocellular, cholestatic or mixed and might also involve immune responses. When DILI occurs in dose-dependent manner, it is referred to as intrinsic, while if the injury occurs spontaneously, it is termed as idiosyncratic. This review predominately focused on idiosyncratic liver injury. The established molecular mechanisms for DILI include (1) mitochondria dysfunction, (2) increased reactive oxygen species levels, (3) presence of elevated apoptosis and necrosis, (4) and bile duct injuries associated with immune mediated pathways. However, it should be emphasized that the underlying mechanisms responsible for DILI are still unknown. Prevention strategies are critical as incidences occur frequently, and treatment options are limited once the injury has developed. The aim of this review was to utilize retrospective cohort studies from across the globe to gain insight into epidemiological patterns. This review considers (1) what is currently known regarding the mechanisms underlying DILI, (2) discusses potential risk factors and (3) implications of the coronavirus pandemic on DILI presentation and research. Future perspectives are also considered and discussed and include potential new biomarkers, causality assessment and reporting methods.

Leveraging multiomics approaches for producing lignocellulose degrading enzymes.

Lignocellulosic materials form the building block of 50% of plant biomass comprising non-chewable agri-components like wheat straw, rice stubbles, wood shavings and other crop residues. The degradation of lignin, cellulose and hemicellulose is complicated and presently being done by chemical process for industrial application through a very energy intensive process. Lignin degradation is primarily an oxidative process where the enzyme lignin peroxidase digests the polymer into smaller fragments. Being a recalcitrant component, higher lignin content poses a challenge of lower recovery of product for industrial use. Globally, the scientists are working on leveraging fungal biotechnology for using the lignocellulose degrading enzymes secreted by actinomycetes and basidiomycetes fungal groups. Enzymes contributing to degradation of lignin are mainly performing the function of modifying the lignin and degrading the lignin. Ligninolytic enzymes do not act as an independent reaction but are vital to complete the degradation process. Microbial enzyme technology is an emerging green tool in industrial biotechnology for commercial application. Bioprocessing of lignocellulosic biomass is challenged by limitations in enzymatic and conversion process where pretreatment and separation steps are done to remove lignin and hydrolyze carbohydrate into fermentable sugars. This review highlights recent advances in molecular biotechnology, lignin valorization, sequencing, decipher microbial membership, and characterize enzyme diversity through 'omics' techniques. Emerging techniques to characterize the interwoven metabolism and spatial interactions between anaerobes are also reviewed, which will prove critical to developing a predictive understanding of anaerobic communities to guide in microbiome engineering This requires more synergistic collaborations from microbial biotechnologists, bioprocess engineers, enzymologists, and other biotechnological fields.

Design and manufacture of one-size-fits-all healthcare face shields for the NHS during the COVID-19 pandemic.

During the COVID-19 pandemic, there was a shortage of personal protective equipment, PPE, which resulted in non-certified PPE being used by healthcare staffs. These would not provide the appropriate protection against the SARS-CoV-2 virus. Together with the local NHS Trust (University Hospitals of Derby and Burton (UHDB) NHS Foundation Trust) and a local small and medium enterprise (SME), Riverside Medical Packaging Ltd, the University of Derby (UoD) developed test protocols for PPE with a one-size-fits-all concept. Building on best practice in reviewing the literature and current design requirements, key design parameters were identified such as a minimum strap width and comfort level for healthcare related Face Shield. Two strap headbands made from fabric and elastomer with linear stiffness of 44.1 ± 0.3 N/m and 149.1 ± 3.1 N/m respectively were tested with respect to fit and comfort on small and large arc-shaped models. There was an exponential change in pressure from the side to the middle of the strap headbands. The high stiffness of the elastomer in a radial set-up influenced the pressure exerted on a wearer's head when the elastomer strap was used. Meanwhile the coefficient of friction between the fabric strap and arc-shaped model influenced the pressure exerted when a fabric strap was used. The ergonomics of the designed Face Shields supported the one-size-fits-all concept, whereby various gender and head circumferences were considered. The findings in this paper will promote new standards in the design of PPE with a one-size-fits-all target.

Cow dung extract mediated green synthesis of zinc oxide nanoparticles for agricultural applications.

In the present study, zinc oxide nanoparticles (ZnO) were synthesized using cow dung extract to apply sustainable agriculture from rural resources. Studies on their antibacterial potential against E. coli DH 5 alpha indicated lower antimicrobial activities than the bulk Zn and commercial Zn nanoparticles. Compared with control and commercial ZnO nanoparticles, the maximum seed germination, root length, and shoot length were observed after the priming of synthesized ZnO NPs. This study suggests that ZnO may significantly increase seed germination and have lower antimicrobial potential. Further, the lower in-vitro cellular leakage and reactive oxygen species (ROS) production provided new hope for using cow dung extract mediated nanoparticles for agricultural and industrial applications.

Phytomelatonin in stress management in agriculture.

Melatonin was discovered as a pineal gland hormone in animals and is now more significantly known as a signaling molecule in plants' biotic and abiotic stressors. Melatonin has been traced back to prokaryotic organisms during evolution and its primary function of antioxidant scavenging free radicals in photosynthetic prokaryotic bacteria is a lesser explored and exciting area for further research globally. The authors at IIT Delhi are trying to establish its potential role in stress management in agriculture. The present manuscript addresses the biosynthetic pathways hitherto suggested by scientists. In this manuscript, the potential scope of melatonin in agriculture as a growth promoter, post-harvest loss inhibitor, and signaling and quality improvement molecule is envisaged.