RESUMEN
Dysfunctional dopamine (DA)-mediated signaling is implicated in several diseases including Parkinson's disease, schizophrenia and attention deficit and hyperactivity disorder. Chronic treatment with DA receptor agonists or antagonists is often used in pharmacotherapy, but the consequences of these treatments on DA neuron function are unclear. It was recently demonstrated that chronic D2 autoreceptor (D2R) activation in DA neurons decreases DA release and inhibits synapse formation. Given that DA neurons can establish synapses that release glutamate in addition to DA, we evaluated the synapse specificity of the functional and structural plasticity induced by chronic D2R activation. We show that chronic activation of the D2R with quinpirole in vitro caused a parallel decrease in the number of dopaminergic and glutamatergic axon terminals. The capacity of DA neurons to synthesize DA was not altered, as indicated by the lack of change in protein kinase A-mediated Ser(40) phosphorylation of tyrosine hydroxylase. However, the spontaneous firing rate of DA neurons was decreased and was associated with altered intrinsic properties as revealed by a prolonged latency to first spike after release from hyperpolarization. Moreover, D2R function was decreased after its chronic activation. Our results demonstrate that chronic activation of the D2R induces a complex neuronal reorganization involving the inhibition of both DA and glutamate synapse formation and an alteration in electrical activity, but not in DA synthesis. A better understanding of D2R-induced morphological and functional long-term plasticity may lead to improved pharmacotherapy of DA-related neurological and psychiatric disorders.
Asunto(s)
Dopamina/metabolismo , Ácido Glutámico/metabolismo , Mesencéfalo/citología , Neuronas , Receptores de Dopamina D2/metabolismo , Sinapsis/fisiología , Animales , Células Cultivadas , Agonistas de Dopamina/metabolismo , Mesencéfalo/metabolismo , Ratones , Ratones Transgénicos , Neurogénesis/fisiología , Neuronas/citología , Neuronas/metabolismo , Técnicas de Placa-Clamp , Quinpirol/metabolismo , Tirosina 3-Monooxigenasa/metabolismoRESUMEN
Activation of protein kinase A (PKA) is known to facilitate synaptic transmission. Using synapses established by hippocampal neurons in culture, we show that dialysis of PKA inhibitors in the presynaptic neuron blocks synaptic facilitation produced by the adenylyl cyclase activator forskolin, demonstrating a presynaptic locus of action. Using ruthenium red, a tool that is known to stimulate exocytosis independently of Ca2+ influx, but in a manner sensitive to tetanus toxin, we find that the secretory process is directly up-regulated under conditions where the number of functional terminals remains unchanged, as revealed by imaging of FM1-43, a vital indicator of synaptic vesicle endocytosis. Taken together with our ultrastructural analysis that suggests no enhancement of docking, our data indicate that PKA causes synaptic facilitation by directly elevating the probability of exocytosis of individual vesicles in response to an invariant Ca2+ signal.
Asunto(s)
Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , Potenciales Evocados/fisiología , Hipocampo/fisiología , Interneuronas/fisiología , Neuronas/fisiología , Sinapsis/fisiología , Transmisión Sináptica/fisiología , Adenilil Ciclasas/metabolismo , Animales , Calcio/metabolismo , Canales de Calcio/fisiología , Células Cultivadas , Colforsina/farmacología , AMP Cíclico/metabolismo , Proteínas Quinasas Dependientes de AMP Cíclico/antagonistas & inhibidores , Endocitosis , Exocitosis , Colorantes Fluorescentes , Interneuronas/efectos de los fármacos , Modelos Neurológicos , Neuronas/citología , Compuestos de Piridinio , Compuestos de Amonio Cuaternario , Ratas , Rojo de Rutenio/farmacología , Sinapsis/efectos de los fármacos , Vesículas Sinápticas/fisiología , Toxina Tetánica/farmacologíaRESUMEN
Chronic blockade or activation of dopamine receptors is critical for the pharmacological treatment of diseases like schizophrenia, Parkinson's or attention deficit and hyperactivity disorder. However, the long-term impact of such treatments on dopamine neurons is unclear. Chronic blockade of the dopamine D2 receptor in vivo triggers an increase in the axonal arborization of dopamine neurons [European Journal of Neuroscience, 2002, 16, 787-794]. However, the specific involvement of presynaptic (autoreceptors) vs. postsynaptic D2 receptors as well as the molecular mechanisms involved have not been determined. Here, we examined the role of D2 autoreceptors in regulating the ability of mouse dopamine neurons to establish axon terminals. Chronic activation of this receptor with quinpirole, a specific agonist, decreased the number of axon terminals established by isolated dopamine neurons. This effect was accompanied by a decrease in dopamine release and was mediated through inhibition of protein kinase A. The decrease in axon terminal number induced by D2 receptor activation was also occluded when the mammalian Target of Rapamycin pathway of mRNA translation was blocked. Our results suggest that chronic activation of the D2 autoreceptor inhibits synaptogenesis by mesencephalic dopamine neurons through translational regulation of the synthesis of proteins required for synapse formation. This study provides a better understanding of the impact of long-term pharmacological interventions acting through the D2 receptor.
Asunto(s)
Dopamina/metabolismo , Mesencéfalo/metabolismo , Neurogénesis/fisiología , Neuronas/metabolismo , Receptores de Dopamina D2/metabolismo , Sinapsis/metabolismo , Animales , Autorreceptores/efectos de los fármacos , Autorreceptores/metabolismo , Proteínas Portadoras/biosíntesis , Proteínas Portadoras/genética , Células Cultivadas , Proteínas Quinasas Dependientes de AMP Cíclico/efectos de los fármacos , Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , Agonistas de Dopamina/farmacología , Mesencéfalo/citología , Mesencéfalo/efectos de los fármacos , Ratones , Ratones Transgénicos , Neurogénesis/efectos de los fármacos , Neuronas/citología , Neuronas/efectos de los fármacos , Fosfotransferasas (Aceptor de Grupo Alcohol)/biosíntesis , Fosfotransferasas (Aceptor de Grupo Alcohol)/genética , Biosíntesis de Proteínas/genética , Quinpirol/farmacología , ARN Mensajero/metabolismo , Transmisión Sináptica/efectos de los fármacos , Transmisión Sináptica/fisiología , Serina-Treonina Quinasas TOR , Factores de Tiempo , Regulación hacia Arriba/efectos de los fármacos , Regulación hacia Arriba/fisiologíaRESUMEN
There is increasing evidence that a subset of midbrain dopamine (DA) neurons uses glutamate as a co-transmitter and expresses vesicular glutamate transporter (VGLUT) 2, one of the three vesicular glutamate transporters. In the present study, double in situ hybridization was used to examine tyrosine hydroxylase (TH) and VGLUT2 mRNA expression during the embryonic development of these neurons, and postnatally, in normal rats and rats injected with 6-hydroxydopamine (6-OHDA) at P4 to destroy partially DA neurons. At embryonic days 15 and 16, there was a regional overlap in the labeling of TH and VGLUT2 mRNA in the ventral mesencephalon, which was no longer found at late embryonic stages (E18-E21) and postnatally. In normal pups from P5 to P15, only 1-2% of neurons containing TH mRNA in the ventral tegmental area (VTA) and substantia nigra, pars compacta, also displayed VGLUT2 mRNA. In contrast, after the cerebroventricular administration of 6-OHDA at P4, 26% of surviving DA neurons in the VTA of P15 rats expressed VGLUT2. To search for a colocalization of TH and VGLUT2 protein in axon terminals of these neurons, the nucleus accumbens of normal and 6-OHDA-lesioned P15 rats was examined by electron microscopy after dual immunocytochemical labeling. In normal rats, VGLUT2 protein was found in 28% of TH positive axon terminals in the core of nucleus accumbens. In 6-OHDA-lesioned rats, the total number of TH positive terminals was considerably reduced, and yet the proportion also displaying VGLUT2 immunoreactivity was modestly but significantly increased (37%). These results lead to the suggestion that the glutamatergic phenotype of a VTA DA neurons is highly plastic, repressed toward the end of normal embryonic development, and derepressed postnatally following injury. They also support the hypothesis of co-release of glutamate and DA by mesencephalic neurons in vivo, at least in the developing brain.
Asunto(s)
Dopamina/metabolismo , Ácido Glutámico/metabolismo , Mesencéfalo/metabolismo , Neuronas/metabolismo , Trastornos Parkinsonianos/metabolismo , Animales , Animales Recién Nacidos , Modelos Animales de Enfermedad , Masculino , Mesencéfalo/citología , Degeneración Nerviosa/metabolismo , Degeneración Nerviosa/patología , Degeneración Nerviosa/fisiopatología , Vías Nerviosas/metabolismo , Vías Nerviosas/patología , Vías Nerviosas/fisiopatología , Plasticidad Neuronal/efectos de los fármacos , Plasticidad Neuronal/fisiología , Núcleo Accumbens/metabolismo , Núcleo Accumbens/patología , Núcleo Accumbens/fisiopatología , Oxidopamina , Trastornos Parkinsonianos/patología , Trastornos Parkinsonianos/fisiopatología , Fenotipo , Terminales Presinápticos/metabolismo , Terminales Presinápticos/patología , ARN Mensajero/metabolismo , Ratas , Ratas Sprague-Dawley , Sustancia Negra/metabolismo , Sustancia Negra/patología , Sustancia Negra/fisiopatología , Simpaticolíticos , Tirosina 3-Monooxigenasa/genética , Área Tegmental Ventral/metabolismo , Área Tegmental Ventral/patología , Área Tegmental Ventral/fisiopatología , Proteína 2 de Transporte Vesicular de Glutamato/genéticaRESUMEN
The physiological and pharmacological properties of dopaminergic neurons in the brain are of major interest. Although much has been learned from cell culture studies, the physiological properties of these neurons remain difficult to study in such models because they are usually in minority and are difficult to distinguish from other non-dopaminergic neurons. Here we have taken advantage of a recently engineered transgenic mouse model expressing enhanced green fluorescence protein (EGFP) under the control of the tyrosine hydroxylase promoter to establish a more effective dopaminergic neuron cell culture model. We first evaluated the specificity of the EGFP expression. Although ectopic expression of EGFP was found in cultures derived from postnatal day 0 pups, this decreased over time in culture such that after 2 weeks, approximately 70% of EGFP-expressing neurons were dopaminergic. We next sought to validate this dopaminergic neuron culture model. We evaluated whether EGFP-expressing dopaminergic neurons displayed some of the well-established properties of dopaminergic neurons. Autoreceptor stimulation inhibited the activity of dopaminergic neurons while neurotensin receptor activation produced the opposite effect. Confocal imaging of the synaptic vesicle optical tracer FM4-64 in EGFP-expressing dopaminergic neurons demonstrated the feasibility of high resolution monitoring of the activity of single terminals established by these neurons. Together, this work provides evidence that primary cultures of postnatal TH-EGFP mice currently represent an excellent model to study the properties of these cells in culture.
Asunto(s)
Dopamina/metabolismo , Ingeniería Genética/métodos , Proteínas Fluorescentes Verdes/genética , Neuronas/metabolismo , Tirosina 3-Monooxigenasa/genética , Potenciales de Acción/efectos de los fármacos , Potenciales de Acción/fisiología , Animales , Animales Recién Nacidos , Autorreceptores/efectos de los fármacos , Autorreceptores/metabolismo , Técnicas de Cultivo de Célula/métodos , Células Cultivadas , Agonistas de Dopamina/farmacología , Ratones , Ratones Transgénicos , Neuronas/efectos de los fármacos , Neurotensina/metabolismo , Neurotensina/farmacología , Técnicas de Placa-Clamp , Terminales Presinápticos/efectos de los fármacos , Terminales Presinápticos/metabolismo , Compuestos de Piridinio , Compuestos de Amonio Cuaternario , Receptores de Dopamina D2/efectos de los fármacos , Receptores de Dopamina D2/metabolismo , Receptores de Neurotensina/efectos de los fármacos , Receptores de Neurotensina/metabolismo , Sustancia Negra/efectos de los fármacos , Sustancia Negra/metabolismo , Área Tegmental Ventral/efectos de los fármacos , Área Tegmental Ventral/metabolismoRESUMEN
Stress effects vary with different environmental situations or stress intensities. The effects of restraint stress on locomotion and or corticosterone were examined. Rats were restrained for 0, 15, 30, 60, 90 or 120 min, subsequent locomotion was measured for 10 min. Rats were also sacrificed for corticosterone determinations. Restraint stress affects both variables. Locomotion was recorded in rats pretreated with naltrexone or vehicle prior to restraint of 15 or 60 min. Naltrexone influenced the effects of stress differentially. It did not affect the results following 15 min of restraint but suppressed locomotion after 60-min restraint to a level comparable to that found after 15 min. Treatment with ethanol (1.0, 1.5, 2.0 g/kg) prior to 15 or 60 min of restraint resulted in the prevention of hypomotility induced by 15-min stress. It also interacted nonadditively with 15-min stress on corticosterone release. No such interaction occurred with 60 min stress. Also, naltrexone made it possible to block the effect of ethanol on restraint-induced hypomotility. Results describe stress as a nonunitary concept. Its effects tend to vary with its duration. The differential interaction of stress with naltrexone and ethanol depending on its duration supports the above notion. Results further suggest recruitment of opioid systems in long duration stress (60 min).
Asunto(s)
Endorfinas/fisiología , Etanol/farmacología , Estrés Psicológico/fisiopatología , Animales , HumanosRESUMEN
Elucidation of the mechanism of action of the atypical antipsychotic clozapine is complicated by the finding that this molecule interacts with multiple targets including dopaminergic and serotonergic receptors. Binding studies have suggested that clozapine also antagonises GABA(A) receptors, but physiological evidence for such a block at functional synapses is lacking. In this study, we explored this antagonism by using electrophysiological techniques on GABAergic neurones of the ventral tegmental area in culture. Inhibitory post-synaptic currents (IPSCs) evoked in isolated GABAergic neurones were found to be dose-dependently inhibited by clozapine. Compatible with a post-synaptic mechanism, we found that membrane currents evoked by exogenous applications of GABA were similarly dose-dependently inhibited by clozapine. An analysis of miniature inhibitory post-synaptic currents (mIPSCs) showed that clozapine reduced the amplitude of quantal events in a way similar to SR-95531, a specific GABA(A) receptor antagonist. Both drugs caused a similar leftward shift of the cumulative probability distribution of mIPSC amplitudes. This suggests that clozapine acts on both synaptic and extrasynaptic GABA(A) receptors. In conclusion, our work demonstrates that clozapine produces a functional antagonism of GABA(A) receptors at synapses. Because this effect occurs at concentrations that could be found in the brain of patients treated with clozapine, a reduction in GABAergic synaptic transmission could be implicated in the therapeutic actions and/or side-effects of clozapine.
Asunto(s)
Clozapina/farmacología , Antagonistas del GABA/farmacología , Neuronas/efectos de los fármacos , Sinapsis/efectos de los fármacos , Transmisión Sináptica/efectos de los fármacos , Área Tegmental Ventral/efectos de los fármacos , Animales , Animales Recién Nacidos , Células Cultivadas , Relación Dosis-Respuesta a Droga , Potenciales de la Membrana/efectos de los fármacos , Neuronas/citología , Neuronas/fisiología , Piridazinas/farmacología , Ratas , Ratas Sprague-Dawley , Receptores de GABA-A/efectos de los fármacos , Receptores de GABA-A/fisiología , Área Tegmental Ventral/citología , Área Tegmental Ventral/fisiologíaRESUMEN
Recent evidence suggests that some types of neurotensin receptors may be expressed by astrocytes. In order to explore the function of neurotensin receptors in astrocytes, the effect of a neurotensin receptor agonist, neurotensin(8-13), on intracellular Ca(2+) dynamics in mixed neuronal/glial cultures prepared from rat ventral tegmental area was examined. It was found that neurotensin(8-13) induces a long-lasting rise in intracellular Ca(2+) concentration in a subset of glial fibrilary acidic protein-positive glial cells. This response displays extensive desensitization and appears to implicate both intracellular and extracellular Ca(2+) sources. In the absence of extracellular Ca(2+), neurotensin(8-13) evokes only a short-lasting rise in intracellular Ca(2+). The neurotensin-evoked intracellular Ca(2+) accumulation is blocked by the phospholipase C inhibitor U73122 and by thapsigargin, suggesting that it is initiated by release of Ca(2+) from an inositol triphosphate-dependent store. The Ca(2+)-mobilizing action of neurotensin(8-13) in astrocytes is dependent on at least two receptors, because the response is blocked in part only by SR48692, a type 1 neurotensin receptor antagonist, and is blocked completely by SR142948A, a novel neurotensin receptor antagonist. The finding that the type 2 neurotensin receptor agonist levocabastine fails to mimic or alter the effects of neurotensin(8-13) on intracellular Ca(2+) makes it unlikely that the type 2 neurotensin receptor is involved. In summary, these results show that functional neurotensin receptors are present in cultured ventral tegmental area astrocytes and that their activation induces a highly desensitizing rise in intracellular Ca(2+). The pharmacological profile of this response suggests that a type 1 neurotensin receptor is involved but that another, possibly novel, non-type 2 neurotensin receptor is also implicated. If present in vivo, such signalling could be involved in some of the physiological actions of neurotensin.
Asunto(s)
Astrocitos/fisiología , Calcio/metabolismo , Neurotensina/farmacología , Fragmentos de Péptidos/farmacología , Receptores de Neurotensina/fisiología , Área Tegmental Ventral/fisiología , Adamantano/análogos & derivados , Adamantano/farmacología , Animales , Animales Recién Nacidos , Astrocitos/citología , Astrocitos/efectos de los fármacos , Células Cultivadas , Estrenos/farmacología , Gadolinio/farmacología , Imidazoles/farmacología , Piperidinas/farmacología , Pirrolidinonas/farmacología , Ratas , Receptores de Neurotensina/antagonistas & inhibidores , Tapsigargina/farmacología , Fosfolipasas de Tipo C/antagonistas & inhibidores , Área Tegmental Ventral/citologíaRESUMEN
The Ca2+ current recorded in the presynaptic neuron (B4/B5) of an identified Aplysia synapse was characterized in terms of its activation, voltage sensitivity, Ca2+ dependence of inactivation and pharmacology. It was compared to that recorded in left upper quadrant abdominal ganglion neurons which, unlike B4/B5, display Ca2+ action potentials. The two Ca2+ currents could not be distinguished in terms of their activation threshold or voltage sensitivity. The Ca2+ current recorded in left upper quadrant neurons, however, displayed more important Ca(2+)-dependent inactivation. The peak Ca2+ current in B4/B5 neurons was significantly reduced (30-40%) by the dihydropyridine Ca2+ channel antagonist, nifedipine, while it was increased (15-20%) by the dihydropyridine Ca2+ channel agonist, BAY K8644, although none of these agents had any effect on transmitter release from B4/B5. omega-Conotoxin similarly reduced the Ca2+ current by 30-40%, but unlike nifedipine, it also caused a 50-60% reduction in B4/B5 transmitter release. The pharmacological properties of the Ca2+ current present in left upper quadrant neurons were somewhat different, as this current was unaffected by either BAY K8644 or omega-conotoxin and moderately suppressed (20%) by nifedipine.
Asunto(s)
Aplysia/metabolismo , Canales de Calcio/metabolismo , Ganglios/metabolismo , Neurotransmisores/metabolismo , Sinapsis/metabolismo , omega-Conotoxinas , Ácido 3-piridinacarboxílico, 1,4-dihidro-2,6-dimetil-5-nitro-4-(2-(trifluorometil)fenil)-, Éster Metílico/farmacología , Potenciales de Acción/efectos de los fármacos , Animales , Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio/efectos de los fármacos , Electrofisiología , Ganglios/efectos de los fármacos , Técnicas In Vitro , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Nifedipino/farmacología , Péptidos Cíclicos/farmacología , Sinapsis/efectos de los fármacosRESUMEN
Changes in evoked acetylcholine quantal release induced by histamine, FLRFamide and buccalin were investigated at an identified neuro-neuronal synapse in the buccal ganglion of Aplysia californica. Regulation of acetylcholine release by these neuromodulators was correlated with their actions on the presynaptic Ca2+ current. We have previously reported that FLRFamide and histamine, respectively, increase and decrease acetylcholine release from buccal neurons B4/B5. Buccalin, a peptide specific to the buccal ganglion, lowered the number of acetylcholine quanta released. Consistent with the synaptic effects, the presynaptic nifedipine-resistant Ca2+ current that triggers the release of acetylcholine in B4/B5 neurons [Trudeau L.-E. et al. (1993) Neuroscience 53, 571-580] was lowered by buccalin or by histamine and enhanced by FLRFamide. The analysis of tail currents showed that histamine shifts the voltage dependence of the nifedipine-resistant Ca2+ channels towards more positive voltages, whereas FLRFamide has an opposite action. Buccalin did not affect the voltage dependence of the channels but depressed the amplitude of the Ca2+ current, an effect which could be due either to a reduction of the number of available Ca2+ channels, to a decrease of their unitary conductance or to a modification of their gating. Inactivation of presynaptic G proteins prevented the modulatory actions of FLRFamide and histamine on quantal acetylcholine release and also on the voltage dependence of the nifedipine-resistant Ca2+ channels. This procedure, however, failed to prevent the suppressive effects of buccalin. The possibility of relating the voltage dependence shifts of the Ca2+ current induced by FLRFamide and histamine to the phosphorylation state of the Ca2+ channels is discussed. It is concluded that three independent presynaptic pathways initiated by histamine, FLRFamide and buccalin control presynaptic Ca2+ influx, these modulations being apparent within the physiological range of voltages required to activate Ca2+ channels.
Asunto(s)
Aplysia/metabolismo , Canales de Calcio/metabolismo , Ganglios/metabolismo , Neurotransmisores/metabolismo , Sinapsis/metabolismo , Animales , Canales de Calcio/efectos de los fármacos , Cimetidina/farmacología , Proteínas de Unión al GTP/metabolismo , Ganglios/efectos de los fármacos , Guanosina Difosfato/análogos & derivados , Guanosina Difosfato/farmacología , Histamina/farmacología , Técnicas In Vitro , Neuropéptidos/farmacología , Nifedipino/farmacología , Oligopéptidos/farmacología , Sistema Nervioso Parasimpático/efectos de los fármacos , Sistema Nervioso Parasimpático/metabolismo , Sinapsis/efectos de los fármacos , Transmisión Sináptica/fisiología , Tionucleótidos/farmacologíaRESUMEN
At an identified neuro-neuronal synapse of Aplysia, 2,5-diterbutyl 1,4-benzohydroquinone, a selective blocker of the reticulum Ca2+ pump, was found to potentiate evoked quantal release of acetylcholine through an increased accumulation of Ca2+ in the presynaptic neuron during depolarization without any accompanying changes in the presynaptic Ca2+ current. We conclude that a rapid Ca2+ buffering system, similar to that associated with the endoplasmic reticulum, must be present in the nerve terminal and play a role in the control of Ca2+ which reaches the release system.
Asunto(s)
Calcio/metabolismo , Retículo Endoplásmico/metabolismo , Hidroquinonas/farmacología , Neuronas/fisiología , Sinapsis/fisiología , Transmisión Sináptica/efectos de los fármacos , Animales , Antioxidantes/farmacología , Aplysia , Ácido Egtácico/farmacología , Potenciales Evocados/efectos de los fármacos , FMRFamida , Ganglios/fisiología , Técnicas In Vitro , Neuronas/efectos de los fármacos , Neuropéptidos/farmacología , Sinapsis/efectos de los fármacosRESUMEN
Dopamine-containing neurones of the ventral tegmental area express neurotensin receptors which are involved in regulating cell firing and dopamine release. Although indirect evidence suggests that some neurotensin receptors may be localised on the nerve terminals of dopaminergic neurones in the striatum and thus locally regulate dopamine release, a clear demonstration of such a mechanism is lacking and a number of indirect sites of action are possible. We have taken advantage of a simplified preparation in which cultured rat ventral tegmental area dopaminergic neurones establish nerve terminals that co-release glutamate to determine whether neurotensin can act at presynaptic sites. We recorded glutamate-mediated synaptic currents that were generated by dopaminergic nerve terminals as an index of presynaptic function. The neurotensin receptor agonist NT(8-13) caused an inward current and an enhancement of the firing rate of dopaminergic neurones together with an increase in the frequency of spontaneous glutamate receptor-mediated excitatory postsynaptic currents (EPSCs). Incompatible with a direct excitatory action on nerve terminals, NT(8-13) failed to change the amplitude of individual action potential-evoked EPSCs or the frequency of miniature EPSCs recorded in the presence of tetrodotoxin. However, NT(8-13) reduced the ability of terminal D2 dopamine receptors to inhibit action potential-evoked EPSCs in isolated dopaminergic neurones. Taken together, our results suggest that in addition to its well-known somatodendritic excitatory effect leading to an increase in firing rate, neurotensin also acts on nerve terminals. The main effect of neurotensin on nerve terminals is not to produce a direct excitation, but rather to decrease the effectiveness of D2 receptor-mediated presynaptic inhibition.
Asunto(s)
Dopamina/metabolismo , Neuronas/fisiología , Neurotensina/fisiología , Terminales Presinápticos/fisiología , Tegmento Mesencefálico/fisiología , Animales , Células Cultivadas , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Potenciales de la Membrana/efectos de los fármacos , Neurotensina/farmacología , Técnicas de Placa-Clamp , Fragmentos de Péptidos/farmacología , Ratas , Receptores de Neurotensina/agonistas , Tegmento Mesencefálico/citología , Factores de TiempoRESUMEN
The involvement of endogenous opioid mechanisms in the interaction between stress and ethanol was investigated in the rat. Animals were pretreated with naltrexone (10 mg/kg) or saline 3 h before a second injection consisting of ethanol (1.0 g/kg) or saline. They were then restrained for 15 or 60 min or left in home cages for an equivalent amount of time. After restraint, animals were either subjected to an open-field test or decapitated to collect blood for corticosterone determinations. Locomotor depression was found to be induced by 15 but not 60 min restraint. In naltrexone-treated animals, however, 60 min restraint was also found to induce locomotor depression. Ethanol pretreatment was found to block the locomotor depression induced by 15 min restraint. Such an interaction was in turn antagonized by naltrexone. In the 15 min condition, stress and ethanol were also found to interact in their effects on plasma levels of corticosterone. Naltrexone did not alter any effects of the stressors on corticosterone levels. These results provide support for the involvement of endogenous opioid mechanisms in the interaction of stress and ethanol at a behavioural level.
Asunto(s)
Endorfinas/fisiología , Etanol/farmacología , Estrés Psicológico/fisiopatología , Animales , Corticosterona/sangre , Etanol/sangre , Masculino , Actividad Motora/fisiología , Naltrexona/farmacología , Ratas , Restricción FísicaRESUMEN
In the present investigation we questioned whether taurine antagonized the effects of ethanol on motor activity measured in the open field. Ten minutes following simultaneous administration (IP) of ethanol (1.0, 1.5, 2.0 and 2.5 g/kg) or saline and taurine (30, 45 and 60 mg/kg) or saline, mice were placed in open field chambers and locomotor activity was measured during a 10 min testing period. A significant interaction was found between taurine and ethanol. Taurine-treated mice displayed lower motor excitation with the 1.0 g/kg dose of ethanol than the saline group treated with the same dose of ethanol. However at the 2.0 g/kg ethanol dose, taurine-treated mice demonstrated higher motor activity than the saline treated mice, once again, treated with the same dose of ethanol. No differences in blood ethanol levels were observed between the two groups. In a second study, taurine administration (30, 45 and 60 mg/kg) did not show any effect on d-amphetamine-induced enhancement of locomotor activity (1, 2, and 3 mg/kg). Data from this study demonstrated an interaction between taurine and ethanol in their effect on locomotor activity in the open field.
Asunto(s)
Etanol/farmacología , Actividad Motora/efectos de los fármacos , Taurina/farmacología , Animales , Dextroanfetamina/farmacología , Interacciones Farmacológicas , Etanol/sangre , Inyecciones Intraperitoneales , RatonesRESUMEN
At an identified neuro-neuronal synapse of the buccal ganglion of Aplysia, quantal release of acetylcholine (ACh) is increased by FMRFamide and decreased by histamine or buccalin. Activation of presynaptic receptors for these neuromodulators modifies a presynaptic Ca2+ current which is nifedipine-resistant and omega-conotoxin-sensitive. The voltage-sensitivity of these N-type Ca2+ channels is increased by FMRFamide and decreased by histamine through the intermediate of G proteins. Buccalin does not implicate G proteins and reduces the Ca2+ current without affecting the voltage-sensitivity of N-type Ca2+ channels. The possibility of relating the shifts in voltage-dependence of the Ca2+ current induced by FMRFamide and histamine to the phosphorylation state of the N-type Ca2+ channels is discussed. A scheme for the complex regulation of ACh release by presynaptic auto- and heteroreceptors is proposed.
Asunto(s)
Acetilcolina/metabolismo , Canales de Calcio/metabolismo , Histamina/metabolismo , Neuropéptidos/metabolismo , Receptores Presinapticos/fisiología , Sinapsis/metabolismo , Animales , Aplysia , FMRFamida , Proteínas de Unión al GTP/fisiología , Histamina/farmacología , Neuronas/fisiología , Neuropéptidos/farmacologíaRESUMEN
In an attempt to investigate the role of cAMP-dependent phosphorylations on synaptic transmission at an Aplysia cholinergic buccal ganglion synapse, the effects of xanthine derivatives such as 3-isobutyl-1-methylxanthine (IBMX), which is well known to inhibit phosphodiesterase activity thereby promoting cAMP accumulation, and a novel xanthine derivative, S-9977-2 were evaluated. They were found to potentiate cholinergic transmission by significantly increasing the time constant of decay (Tc) of inhibitory postsynaptic currents (IPSCs). The postsynaptic origin of the phenomenon was supported by the observation that responses to the ionophoretic application of acetylcholine (ACh) were also potentiated in duration as well as in amplitude. No effects of S-9977-2 on the ACh-gated Cl- channel conductance or mean open time were observed. The finding that responses to the hydrolysis-resistant cholinergic analogue carbachol were unaffected by the two xanthines suggested that the observed effects were at least partly caused by an inhibition of acetylcholinesterase (AChE) activity. That these substances inhibit AChE activity was confirmed in vitro. Phosphorylation processes nonetheless appear to be partly involved in the synaptic effect of the xanthines as the kinase blocker H-8 blocked part of the IPSC Tc lengthening. Possible mechanisms are discussed.
Asunto(s)
1-Metil-3-Isobutilxantina/farmacología , Encéfalo/fisiología , Sistema Nervioso Parasimpático/fisiología , Piperazinas/farmacología , Sinapsis/fisiología , Transmisión Sináptica/efectos de los fármacos , Xantinas/farmacología , Acetilcolina/farmacología , Acetilcolinesterasa/metabolismo , Animales , Aplysia , Carbacol/farmacología , ElectrofisiologíaRESUMEN
Antipsychotic drugs such as haloperidol act as dopamine D2 receptor antagonists to produce a number of cellular effects including the induction of immediate-early genes such as c-fos. It has been hypothesized that blockade of D2 receptors by antipsychotics is responsible for the induction of c-fos, but the mechanism has not been determined. Using cultured ventral tegmental area (VTA) dopaminergic neurons as a model, we report that nanomolar concentrations of haloperidol cause a time-dependent increase in Fos expression in dopaminergic neurons.Surprisingly, this induction was not mimicked by sulpiride, a selective D2 receptor antagonist, and was not blocked by Rp-cAMPS, an antagonist of protein kinase A (PKA), thus suggesting that D2 receptors and the cAMP cascade are not required. The induction of Fos expression was blocked by tetrodotoxin, BAPTA and KN-93, thus showing that it is activity- and calcium-dependent and requires the activation of a calmodulin-dependent kinase (CaMK). Together, these results suggest that haloperidol induces Fos expression in dopaminergic neurons through a D2 receptor-independent increase in intracellular calcium, leading to CaMK activation.
Asunto(s)
Calcio/fisiología , AMP Cíclico/análogos & derivados , Dopamina/fisiología , Expresión Génica/efectos de los fármacos , Genes fos/efectos de los fármacos , Haloperidol/farmacocinética , Neuronas/fisiología , Receptores de Dopamina D2/biosíntesis , Animales , Bencilaminas/farmacología , Proteínas Quinasas Dependientes de Calcio-Calmodulina/antagonistas & inhibidores , Proteínas Quinasas Dependientes de Calcio-Calmodulina/efectos de los fármacos , Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , Células Cultivadas , Colforsina/antagonistas & inhibidores , Colforsina/metabolismo , AMP Cíclico/metabolismo , AMP Cíclico/farmacología , Expresión Génica/genética , Genes fos/genética , Haloperidol/administración & dosificación , Haloperidol/antagonistas & inhibidores , Neuronas/efectos de los fármacos , ARN Mensajero/metabolismo , Ratas , Ratas Sprague-Dawley , Receptores de Dopamina D2/efectos de los fármacos , Receptores sigma/efectos de los fármacos , Receptores sigma/metabolismo , Sistemas de Mensajero Secundario/efectos de los fármacos , Sistemas de Mensajero Secundario/fisiología , Transducción de Señal , Sulfonamidas/farmacología , Tetrodotoxina/farmacología , Tionucleótidos/metabolismo , Tionucleótidos/farmacología , Área Tegmental Ventral/citología , Área Tegmental Ventral/efectos de los fármacos , Área Tegmental Ventral/fisiologíaRESUMEN
The interaction between restraint-stress and ethanol was investigated in the rat. The effects of ethanol pretreatment (0.0, 1.0, 1.5, 2.0 g/kg, 20% v/v) on locomotor depression and corticosterone release induced by restraint-stress (15, 60 min) were measured. Restraint durations of 15, 30, 90 and 120 min were found to decrease locomotor activity while animals restrained for 60 min did not differ from home cage controls. All restraint durations induced a significant increase in plasma levels of corticosterone. Locomotor activity counts of ethanol-pretreated (1.0, 1.5, 2.0 g/kg; 20% v/v) animals restrained for 15 min were not found to be lower than those of ethanol-pretreated animals remaining in home cages. Ethanol pretreatment did not differentially affect the locomotor activity of restrained or home cage animals in the 60-min condition. Plasma corticosterone levels of ethanol-pretreated animals restrained for 15 min were identical to those of ethanol-pretreated home cage controls. However, ethanol-pretreated animals restrained for 60 min demonstrated plasma corticosterone levels higher than those obtained by ethanol pretreatment or 60-min restraint alone. Blood ethanol levels were not found to be different between ethanol-control and ethanol-stress animals. These results provide support for a stress-ethanol interaction. They also suggest a differential interaction of ethanol with different intensities of stress.
Asunto(s)
Corticosterona/sangre , Etanol/farmacología , Actividad Motora/efectos de los fármacos , Estrés Fisiológico/psicología , Animales , Etanol/sangre , Masculino , Ratas , Restricción Física , Estrés Fisiológico/sangre , Estrés Fisiológico/etiología , Factores de TiempoRESUMEN
DCC (deleted in colorectal cancer), a receptor for the axon guidance cue netrin-1, is highly expressed by mesencephalic dopaminergic (DA) neurons during development; however, the contribution of DCC to DA development remains largely uncharacterized. DA neurons in ventral mesencephalic nuclei also express UNC5 homologue netrin receptors from late embryogenesis to adulthood, raising the possibility that DA axons could be attracted or repelled by netrins. Examining newborn dcc null mice, we report that loss of DCC function results in profound alterations of DA circuitry, including DA progenitor cell migration defects, reduced numbers of DA cells in midbrain nuclei, an anomalous DA ventral commissure, malformed DA innervation of the ventral striatum, and reduced DA innervation of the cerebral cortex. Caspase-3 activation was detected in inappropriately localized DA cells, consistent with apoptosis contributing to reduced cell numbers. Dcc heterozygous mice express reduced levels of DCC protein. Although less severely disrupted than dcc nulls, newborn and adult dcc heterozygotes also have fewer DA neurons in ventral mesenscephalic nuclei. Despite the reduced numbers of DA neurons, newborn dcc heterozygotes and nulls exhibit similar DA innervation density as wild-type littermates in the nucleus accumbens core, and adult dcc heterozygotes exhibit increased DA innervation in medial prefrontal cortex. A trend towards increased innervation of medial prefrontal cortex was detected in newborn dcc heterozygotes, but did not reach statistical significance, suggesting that the increase in adult heterozygotes results from enhanced DA arborization during postnatal development. Consistent with the hypothesis that DCC regulates DA axonal projections, disrupting DCC function in culture inhibits netrin-1 induced DA axon extension and axon branching. Furthermore, disrupting DCC function in isolated DA neurons grown as micro-island cultures reduces the number of autaptic synapses per cell. We conclude that DCC regulates appropriate precursor cell migration, axon guidance, and terminal arborization by DA neurons.
Asunto(s)
Axones/fisiología , Encéfalo/fisiología , Dopamina/fisiología , Neuronas/fisiología , Receptores de Superficie Celular/fisiología , Células Madre/fisiología , Proteínas Supresoras de Tumor/fisiología , Animales , Animales Recién Nacidos , Encéfalo/citología , Movimiento Celular , Células Cultivadas , Receptor DCC , Ratones , Ratones Noqueados , Receptores de Superficie Celular/genética , Sinapsis/fisiología , Proteínas Supresoras de Tumor/genética , Tirosina 3-Monooxigenasa/metabolismoRESUMEN
Several lines of evidence suggest a close association between dopamine (DA) and neurotensin (NT) systems in the CNS. Indeed, in the rodent brain, abundant NT-containing fibres are found in DA-rich areas such as the ventral tegmental area and substantia nigra. Moreover, it has been shown in vivo that NT, acting through its high-affinity receptor (NTS1), reduces the physiological and behavioural effects of DA D2 receptor (D2R) activation, a critical autoreceptor feedback system regulating DA neurotransmission. However, the mechanism of this interaction is still elusive. The aim of our study was thus to reproduce in vitro the interaction between D2R and NTS1, and then to characterize the mechanisms implicated. We used a primary culture model of DA neurons prepared from transgenic mice expressing green fluorescent protein under the control of the tyrosine hydroxylase promoter. In these cultures, DA neurons endogenously express both D2R and NTS1. Using electrophysiological recordings, we show that activation of D2R directly inhibits the firing rate of DA neurons. In addition, we find that NT, acting through a NTS1-like receptor, is able to reduce D2R autoreceptor function independently of its ability to enhance DA neuron firing, and that this interaction occurs through a protein kinase C- and Ca(2+)-dependent mechanism. Furthermore, prior activation of D2R reduces the ability of NTS1 to induce intracellular Ca(2+) mobilization. Our findings provide evidence for bidirectional interaction between D2R and NTS1 in DA neurons, a regulatory mechanism that could play a key role in the control of the activity of these neurons.