Testis-specific actin-like 7A (ACTL7A) is an indispensable protein for subacrosomal-associated F-actin formation, acrosomal anchoring, and male fertility.

Formation of the acrosome during spermiogenesis is an essential process for creating fertilization-competent sperm. Of the numerous aspects required for acrosome biogenesis, adherence of the acrosomal outer membrane to the nuclear surface is mediated by the subacrosomal perinuclear theca. However, the cellular dynamics and congruent functions pertaining to these acrosomal anchoring factors are not well understood despite many of them being implicated as potential causes for human male infertility. Actin-like 7A (ACTL7A) is one such factor for which deleterious polymorphisms have recently been shown to cause human male infertility. It is thought that acrosomal attachment is coordinated by cytoskeletal associations between the acrosome and nucleus via the acroplaxome. To further illuminate the mechanistic underpinnings of ACTL7A for essential acrosome associations, in this study, we investigated its dynamic localization in the developing germline, molecular associations with other cytoskeletal components, and the cellular consequences of ablation. Our intracellular localization data show ACTL7A to be dynamically present within the nucleus and subacrosomal space and later associated with postacrosomal regions of developing spermatids. Through the generation of an Actl7a knock-out mouse model, we consistently observed disruption of acrosomal biogenesis with abnormal migration of the acrosomal granule and peeling acrosomes during spermatid elongation. Significantly, we found a complete loss of subacrosomal filamentous actin (F-actin) structures in knock-out spermatids suggesting a regulatory role for subacrosomal F-actin. Considering our reported data together with existing literature, we propose a mechanistic model explaining the essential role of ACTL7A for acroplaxome-associated F-actin, acrosomal attachment integrity, and male fertility.

Multivariable modeling, optimization and experimental study of Cr(VI) removal from aqueous solution using peanut shell biochar.

Peanut shell biomass was selected and utilized to produce biochar through pyrolysis under N2 atmosphere at 923 K. After studying various effects of experimental parameters and by statistical modeling and optimization by RSM using Box-Benken design, optimized conditions of pH 2.0 ± 0.1, temperature 303 K, and adsorbent dose used of 2.5 g L-1 were obtained giving almost 99.99% removal for Cr(VI) from the solution. FESEM, FTIR, XRD, XPS, EDX, elemental mapping, and pHzpc were used for the evaluation of the surface characteristics of peanut shell biochar (PSB). Studies revealed C-O, C-H, CO, and O-H functional groups' presence with the help of FTIR, majorly in control of adsorption mechanism and the EDX confirmed the presence of Cr(VI) onto peanut shell biochar (PSB). Further adsorption mechanism for Cr(VI) adsorption followed the pseudo-second-order rate with adsorption capacity of 29.38 mg g-1 given by the Langmuir isotherm. The thermodynamic study confirmed the exothermic and spontaneous nature of the process for Cr(VI) adsorption onto PSB. The adsorption mechanism showed electrostatic attraction, reduction, and complexation mainly responsible for Cr(VI) adsorption by PSB. Thus, PSB effectively removes Cr(VI) is confirmed by the present study.

Comparative haemato-biochemical profile of induced anchylostomiasis in healthy and diabetic murine model.

A study aims to determine comparative pathology of induced anchylostomiasis was conducted in murine model. L3 larvae of Ancylostoma caninum were given at the rate of 200 larvae per rat orally and 1000 per rat through per cutaneous route in both healthy and diabetic groups as per the design of the experiment. Blood samples were collected on day 15, 30 and 45 for haemato-biochemical investigations. Decrease in Hb, PCV, TEC was observed in all groups as compared to healthy control. Leukocytosis with neutrophilia and eosinophilia was also recorded. An increase in glucose, cholesterol, ALT and AST was recorded in diabetic groups as compared to their counterparts. BUN and creatinine was elevated in all infected group. Similarly total protein and albumin gradually declines (after initial increase) in all the groups. Most of the parameters remain unaffected in group V as compared to control. It was concluded that the pathology of induced anchylostomiasis in murine model is more sever in diabetic group as evident by the significantly altered haemato-biochemical parameters when compare to their counterparts. This may be due to damage caused by non treated diabetes to different body system in addition to the anchylostomiasis.

Mechanistic and recent updates in nano-bioremediation for developing green technology to alleviate agricultural contaminants.

The rise in environmental pollutant levels in recent years is mostly attributable to anthropogenic activities such as industrial, agricultural and other activities. Additionally, these activities may produce excessive levels of dangerous toxicants such as heavy metals, organic pollutants including pesticide and herbicide chemicals, and sewage discharges from residential and commercial sources. With a focus on environmentally friendly, sustainable technology, new technologies such as combined process of nanotechnology and bioremediation are urgently needed to accelerate the cost-effective remediation process to alleviate toxic contaminants than the conventional remediation methods. Numerous studies have shown that nanoparticles possess special qualities including improved catalysis and adsorption as well as increased reactivity. Currently, microorganisms and their extracts are being used as promising, environmentally friendly catalysts for engineered nanomaterial. In the long term, this combination of both technologies called nano-bioremediation may significantly alter the field of environmental remediation since it is more intelligent, safe, environmentally friendly, economical and green. This review provides an overview of soil and water remediation techniques as well as the use of nano-bioremediation, which is made from various living organisms. Additionally, current developments related to the mechanism, model and kinetic studies for remediation of agricultural contaminants have been discussed.

Emergence Time and Sequence of Primary Teeth in a Sample of Nepalese Children.

Background Primary teeth emergence is an important milestone in children and timing of emergence varies among populations. Objective To determine the emergence time and sequence of primary teeth in a sample of Nepalese children visiting Dhulikhel Hospital. Method A descriptive cross-sectional study was conducted in children of 5 months to 4 years visiting Dhulikhel Hospital. The emergence of incisal tip to incisal margin for incisors and canines, cusp tip to occlusal margin of molars visible were recorded along with age in months and gender. Descriptive statistics was done to calculate the mean age of emergence of each tooth with standard deviation. Unpaired t-test was used to assess the difference between the mean age of emergence of teeth between right and left sides and between boys and girls. Result The first teeth to emerge was mandibular central incisor at the age of 9.37 ± 1.42 months and the last one was maxillary second molar at the age of 32.91 ± 6.39 months. There was no significant difference in the mean emergence time between the maxillary and mandibular jaws, between right and left sides of jaws and between boys and girls except for primary maxillary right central incisor and mandibular right second molar which was found to be emerged early in girls. Conclusion The emergence time and sequence of primary teeth observed in the present study can be used as a baseline data for the children of Kavre district.

Molecular characterization of N-methyl-d-aspartate receptor from Bemisia tabaci.

The N-methyl-d-aspartate receptors (NMDARs) are ionotropic ligand gated channels that are highly permeable to calcium ions. In insects, NMDARs are associated with glutamatergic neurotransmission governing diverse physiological and biological processes like vitellogenesis and ovarian development. Therefore, NMDAR may act as attractive target for insect pest control. In present study, we performed structural and functional characterization of NMDARs in Bemisia tabaci, a highly invasive crop pest and potent virus vector. We identified that NMDAR consists of three subunits each encoded by single gene in whiteflies which are highly conserved among different insect orders. Expression analysis suggests that subunit 1 (BtNR1) and subunit 2 (BtNR2) are the main functional units. External supplementation of NMDAR ligand or BtNRs silencing was lethal to insects, which suggested that NMDAR function is highly balanced in whiteflies.

Translation and Validation of Oral Health-Related Early Childhood Quality of Life Tool for Nepalese Preschool Children.

Background Early childhood caries (ECC) in preschool children debilitate their quality of life affecting oral function, appearance and social well-being. A tool for measuring oral health-related quality of life for preschoolers may help in making clinical decisions and assist policy makers in planning and management of health programs in the country. Objective To translate oral health-related early childhood quality of life (OH-ECQoL) tool into Nepali language and test its validity and reliability. Method OH-ECQoL was translated into Nepali language by two Nepalese professional translators which was back translated by professional English translator. This was sent to three independent advisors to see the appropriateness of translation. Pilot testing was done in 20 parents and questionnaire was finalized after needed corrections. Final version was introduced to the 118 parents of children aged 24- 71 months. Caries status was recorded for all children participating in the study. Concurrent, construct, discriminant validity and internal consistency reliability, test -retest reliability were evaluated. Result OH-ECQoL scores and perception of parents for general and oral health of their children was significant (at 0.01 level). There was significant difference in OH-ECQoL scores between no ECC and severe ECC groups and moderate and severe ECC groups (at 0.05 level). There was also a significant correlation between child impact section and family impact section (at 0.01 level). Cronbach's alpha was 0.891 demonstrating good internal consistency. Intra class coefficient was 0.963 suggesting excellent testretest reliability. Ninety-one (77.1%) children had severe ECC and 40(33.9%) parents were from upper middle class. Conclusion The Nepali version of OH-ECQoL is a valid and reliable tool for assessing the oral health-related early childhood quality of life in children of Nepal.

Developing tool and Measuring Integration Characteristics of Basic Science Curriculum to Improve Curriculum Integration.

Background Integrated curriculum enhances students' learning and the retention of knowledge. At Patan Academy of Health Sciences, integrated organ system based curriculum is used with Problem Based Learning as the principle teaching learning method to foster students' learning. In addition, other approaches of integration were under taken such as integrated assessment; logical arrangement teaching learning methods (lectures and practicals); joint effort of faculty in curriculum planning and delivery; conducive environment to foster hidden curriculum. This study describes the perception of faculty and students on integration characteristics of Basic Science curriculum. Objective To measure the integration characteristics of Basic Science Curriculum. Method Tool was developed to measure integration characteristics of Basic Science Curriculum and ensure whether such planned integration has been achieved. Mixed method was used to measure the perception of the integration characteristics i) quantitatively by questionnaire survey to faculty and students ii) qualitatively by in-depth interview of students. Result Both faculty and students perceived that all the blocks in Basic Science was well integrated in the quantitative questionnaire survey. But, in the in-depth interview, students perceived integration of curriculum in organ system blocks were better integrated compared to Principle of Human Biology blocks where fundamentals of basic science disciplines were delivered. Students reflected that Problem Based Learning not only integrated Basic Science disciplines but also with clinical sciences and the social context. But, students perceived that Community Health Sciences curriculum was not so well integrated with Basic Science subjects. Conclusion Overall, this study showed that planned integration in Basic Science curriculum was successfully executed during curriculum implementation.

An Experience of Pediatric Upper and Lower Gastrointestinal Endoscopy in a Tertiary Center.

Background Although upper gastrointestinal endoscopy and colonoscopy is considered to be a gold standard in the diagnosis of many pediatric gastrointestinal disorders, there is limited data about its utility from our country. This important diagnostic tool is underutilized. Objective The objective of this study is to report the common indications, endoscopic findings and complications of pediatric upper gastrointestinal endoscopy and colonoscopy in a tertiary center to increase awareness amongst pediatricians. Methods This descriptive cross-sectional study was conducted in children undergoing upper gastrointestinal endoscopy and colonoscopy for various indications in a tertiary center from November 2014 to October 2016 (24 months). Results We performed 192 endoscopies during this period. Upper gastrointestinal endoscopy 158 and Colonoscopy 34. Mean age of patients was 10.2 years (range 6 months to 16 years) and 140(72.9%) patients were under 10 years of age. Common indications of UGI endoscopy were recurrent abdominal pain (67%), acute abdominal pain (12.6%), recurrent vomiting (8.0%), upper GI bleed (4.4%), failure to thrive (3.1%) and caustic ingestion (2.5%). An abnormality was detected in 128(81%) patients. Antral gastritis (55%) was the most common diagnosis. Thirty two percent of children with RAP had chronic moderate to severe gastritis and were positive for H. pylori in Giemsa stain on histopathological examination. Common indications of colonoscopy were Lower GI bleed and chronic diarrhea. Out of 22 patients who underwent colonoscopy for lower GI bleed, 18(82%) patients had rectal polyp and underwent snare polypectomy. Out of 12 patients who were evaluated for chronic diarrhea, 7(20%) were diagnosed to have cow's milk protein allergy and 5(15%) patients had inflammatory bowel disease. An abnormality was detected in 30(88%) patients. All children received sedation/ analgesia and tolerated the procedure well. Conclusion Upper gastrointestinal endoscopy and colonoscopy are safe procedure in children. The awareness about its diagnostic and therapeutic role should be raised amongst pediatricians in developing countries. There is also a need to develop training programs of pediatric gastroenterology and pediatric endoscopic suites in developing countries so that children may benefit from this state of the art diagnostic modality.

Assessing the Relationship of Maternal Factors and Family Income with Early Childhood Caries: A Hospital Based Study.

Background Earlychildhood caries (ECC) is a multifactorial disease. Maternal factors and family income has major influence on the caries status of children. Objective To assess the relationship of maternal factors and family income with early childhood caries among children attending Pediatric Dentistry department of Dhulikhel Hospital. Method A cross sectional study was conducted among 239 children of age 3 to 6 years attending Pediatric Dentistry department of Dhulikhel Hospital, Kavre, Nepal. Age of child, mother's occupational status,educational level and family income were recorded. Oral examination of child was done to record decayed, missing, filled teeth (dmft). Descriptive analysis was done to observe the caries experience. Chi - square test was used to assess the relationship of caries experience with occupational status, education of mother and family income. Result The mean decayed, missing, filled teeth of the children was7.04±4.10. There was no significant relationship of early childhood caries with occupational status of mother (p=0.675), education of mother (p=0.140) and family income (p=0.158). Conclusion There was no relationship of maternal factors and family income with early childhood caries when surveyed among the children visiting Pediatric Dentistry department of Dhulikhel hospital.

Gentiana lutea exerts anti-atherosclerotic effects by preventing endothelial inflammation and smooth muscle cell migration.

BACKGROUND AND AIMS: Studies suggest that Gentiana lutea (GL), and its component isovitexin, may exhibit anti-atherosclerotic properties. In this study we sought to investigate the protective mechanism of GL aqueous root extract and isovitexin on endothelial inflammation, smooth muscle cell migation, and on the onset and progression of atherosclerosis in streptozotocin (STZ)-induced diabetic rats. METHODS AND RESULTS: Our results show that both GL extract and isovitexin, block leukocyte adhesion and generation of reactive oxygen species in human umbilical vein endothelial cells (HUVECs) and rat aortic smooth muscle cells (RASMCs), following TNF-alpha and platelet derived growth factor-BB (PDGF-BB) challenges respectively. Both the extract and isovitexin blocked TNF-α induced expression of ICAM-1 and VCAM-1 in HUVECs. PDGF-BB induced migration of RASMCs and phospholipase C-γ activation, were also abrogated by GL extract and isovitexin. Fura-2 based ratiometric measurements demonstrated that, both the extact, and isovitexin, inhibit PDGF-BB mediated intracellular calcium rise in RASMCs. Supplementation of regular diet with 2% GL root powder for STZ rats, reduced total cholesterol in blood. Oil Red O staining demonstrated decreased lipid accumulation in aortic wall of diabetic animals upon treatment with GL. Medial thickness and deposition of collagen in the aortic segment of diabetic rats were also reduced upon supplementation. Immunohistochemistry demonstrated reduced expression of vascular cell adhesion molecule-1 (VCAM-1), inducible nitric oxide synthase (iNOS), and vascular endothelial cadherin (VE-cadherin) in aortic segments of diabetic rats following GL treatment. CONCLUSIONS: Thus, our results support that GL root extract/powder and isovitexin exhibit anti-atherosclerotic activities.

Permanent Teeth Emergence Time and Sequence in Children of Kavre District, Nepal.

Background Population specific standards on the timing and sequence of emergence of permanent teeth are essential in planning dental care for children. There is only a single study on the emergence of permanent teeth in Nepalese children. Objective To determine the standards for permanent teeth emergence time and sequence in children of Kavre district, Nepal. Method A descriptive cross sectional study was conducted in 623 children of age 5 to 14 years from six different schools of Kavre district. The number of permanent tooth erupted except third molar was recorded along with age and gender. Data were analysed using SPSS 20.0. Descriptive statistics was used to determine the number and percentage of permanent teeth emerged at different ages and the mean age of emergence of each tooth. Student 't' test was used to determine the inter jaw differences in the mean age of emergence of each tooth. Result The study population constituted 50.7% boys and 49.3% girls of the total sample. The first tooth to emerge was mandibular first molar, whereas maxillary second molar tended to be the last to emerge in both the genders. Though in general, mandibular teeth tended to precede the corresponding maxillary teeth in emergence but significantly only mandibular central, lateral and second premolar emerged earlier than maxillary.( p ≤ 0.05) Conclusion This study can be used as a reference data for clinical and academic purpose especially for the children of Kavre district, Nepal.

A new species of Neolebouria Gibson, 1976 (Opecoelidae: Plagioporinae) from the whitecheek monocle bream, Scolopsis vosmeri (Perciformes: Nemipteridae), from the Panjim coast at Goa, with a checklist of parasites previously reported from this fish.

Neolebouria capoori n. sp. (Opecoelidae: Plagioporinae) is described from the whitecheek monocle bream, Scolopsis vosmeri (Bloch) (Perciformes: Nemipteridae) from the Panjim coast on the central west coast of India at Goa. The new species differs from both Neolebouria cantherhini (Li, Qiu & Zhang, 1988) as originally described from Thamnaconus modestus (Günther) (syn. Cantherines modestus Günther ) and Neolebouria confusum (Overstreet, 1969) as originally described from Ocyurus chrysurus (Bloch) by having the cirrus sac surpassing the ventral sucker posteriorly in N. cantherhini and being entirely preacetabular in N. confusum compared to terminating near the midlevel of the ventral sucker in N. capoori n. sp. The new species is most similar to N. confusum, but it further differs from this species by having the vitelline fields terminating near the level of the esophageal bifurcation compared to terminating near the level of the posterior margin of the pharynx, a larger sucker ratio (1:1.7-1:2.0 compared to 1:1.4-1:1.7), a somewhat shorter cirrus sac relative to body length (160-448, representing 9-18% of the body length compared to about 367, representing 22%), and the egg of the new species has a boss at the anopercular end that is not present in N. confusum. This study represents the first report on an opecoelid from S. vosmeri. A review of the parasites reported from S. vosmeri is included.

Limnochemistry and nutrient dynamics in Upper Lake, Bhopal, India.

In limnetic ecosystem, water quality depends upon physical, chemical, and biological factors. Effects of temperature, light scattering, and absorption by suspended and dissolved matter, transport, and mixing of nutrients within the lake are the significant factors as far as water quality is concerned. Nutrient loading into the lake and internal cycling of nutrients is always a matter of concern and critical to number of processes. During the winter season, heat and momentum transfer at the lake surface and the temperature-density relation of water destabilize the water column and drive vertical mixing and transport processes. The deepening of the surface layer produces nutrient transfer from the hypolimnion into the euphotic zone of epilimnion. It may also resuspend sediments that would have settled under stratified conditions, or redistribute particles that may still be in suspension. Thus, there exists a complex connection between the hydrodynamics and water quality issues. Present study is an effort to understand how seasonal changes in the limnetic ecosystem regulate the limnochemistry and movement of nutrient. The study revealed that significant variations of nutrients and organic load were observed between epilimnion and hypolimnion during summer season, and the lake was found in hyper-eutrophic condition throughout the study period.

Effect of incorporation of soy protein isolate and inulin on quality characteristics and shelf-life of low-fat duck meat sausages.

OBJECTIVE: Low fat duck meat sausages were prepared by replacing the fat in the formulations with soy protein isolate (SPI) and inulin to find the best formulation having superior shelflife without affecting its quality attributes. METHODS: Four sausage mix formulations were prepared viz.control (0% SPI and inulin), T1 (2.5% inulin), T2 (2.5% SPI), and T3 (2.5% SPI+2.5% inulin) replacing duck fat as per the recipe. Five batches of duck meat sausages of each formulation were prepared, and the final products were evaluated for physico-chemical, organoleptic, and microbiological qualities. RESULTS: The % moisture and crude protein content of the sausages revealed an increasing trend (p<0.01) from control to the treated formulations, while the % total ash contents were found to be non-significant (p>0.05). On the contrary, the per cent ether extract decreased significantly (p<0.01) from the control to the treated groups. In terms of calorie value, control samples exhibited the highest values with a significant (p<0.01) regression from control to treated formulation, respectively. The colour profile study (L, a*, b*) of the formulations were found to be non-significant. Texture profile study in terms of springiness, cohesiveness, chewiness, and resilience revealed no significant difference in all the treatment groups except the hardness scores, which revealed a significantly (p<0.01) increasing trend from control to the treated formulations. The total viable count showed a significant decrease in the treated groups. However, there was a significant increase in the bacterial load during the storage till day 15th. The total viable psychrophilic bacterial count showed a significant (p<0.01) increase in bacterial load from day 5th to 15th day of storage. Colititre counts were negative for all the formulations until the 15th day of storage. CONCLUSION: The present study results may conclude that duck meat sausages could be prepared satisfactorily by replacing duck fat with SPI and inulin at the rate of 2.5% of each with superior quality attributes.

A spontaneous teratocarcinoma in the testis of a swiss albino mouse.

A unilateral non-metastatic embryonal carcinoma and teratoma of the testis was observed in a 12-week-old Swiss Albino mouse at the end of a 28-day repeated dose toxicity study. The teratocarcinoma almost completely replaced the parenchyma of the left testis. The tumor was composed of sheets and rosettes of primitive embryonal cells, anaplastic cells, skeletal muscle tissue, sebaceous gland tissue, keratinized stratified squamous epithelium, and ciliated cuboidal epithelium. The histomorphological characteristics of the tumor were reviewed and presented in this report. To the best of the authors' knowledge, this is the first report of spontaneous teratocarcinoma of testis in the Swiss Albino strain of mice.

Nanoionomer: evaluation of microleakage.

BACKGROUND: Glass ionomer cements are widely used in pediatric practice due to their advantage of fluoride release and chemical bond to tooth structure. Adherence of the restorative material to the cavity walls is one of the most important characteristic for it to be proven as an ideal material as it prevents microleakage. AIMS AND OBJECTIVES: This study was aimed at evaluating the microleakage of nanofilled resin-modified glass ionomer cement compared with the conventional and resin-modified glass ionomer cements. MATERIALS AND METHODS: Standard class V cavities of size 3 mm x 2 mm x 2 mm were made on a total of 30 extracted teeth and restored with the conventional glass ionomer, resin-modified glass ionomer or nanoionomer. After thermocycling, teeth were immersed in 0.5% methylene blue dye for 24 h. They were then sectioned buccolingually. Microleakage was assessed for the occlusal and gingival walls using a compound microscope by two examiners independently. RESULTS: Nanoionomer demonstrated the least microleakage, with a mean score of 1.3, compared with the resin-modified glass ionomer (score of 3.2) and conventional glass ionomer cement (score 2.6). CONCLUSION: Nanoionomer exhibited adequate resistance to microleakage and thus may prove better than conventional or resin-modified glass ionomers.

Advance spectral approach for condition evaluation of rolling element bearings.

The Performance of rolling element bearings has a significant influence on reliability and safety in the various engineering fields. While heading toward the condition evaluation of rolling element bearings, the higher dimensionality of feature space became a crucial concern. Thus, to have a check on the hurdles, this paper proposes a novel approach of bearing condition assessment utilizing Local mean decomposition (LMD) and spectral clustering (SC). Spectral Clustering (SC) is an influential tool, which heals the curse of dimensionality and facilitates the model by imparting a systematized structure. SC focuses more on connectivity rather than geometrical vicinity; moreover, the provision of a similarity matrix makes SC more reliable and increases its performance. To enrich the efficiency of SC, a novel decision criterion, PI (Parting Index) aiming the optimal number of clusters based on a new similarity indicator and disorder indicator coined as Synergic Association Index (SAI) and Separation Index (SI) respectively, has been proposed. The work aims to achieve better performance degradation assessment (PDA) by the application of the new SC-PI approach with the following steps; the first step is the decomposition of vibration signal into product functions (PFs) utilizing LMD; the second step involves the extraction of proper fault features; third, the extracted features are classified with the SC-PI method; finally the feature or attribute vectors are endowed to trained model and confidence value (CV) is calculated. The proposed method is validated on two datasets of different fault types. The results so obtained indicates that the SC-PI method easily differentiates the various stages when compared to different assessment techniques i.e. time-domain features, self-organizing map (SOM), k-medoids and Gaussian mixture model (GMM).

Effect of Montmorillonite clay on pyrolysis of paper mill waste.

The thermal degradation of paper mill waste (PMW) has been investigated in presence and absence of Montmorillonite clay in the temperature range of ambient to 1000 °C and at the heating rates of 20 °C/min, 25 °C/min and 30 °C/min. Proximate and ultimate analyses and evaluation of calorific value (HHV) of PMW have been carried out using standard protocols. The thermo-gravimetric analysis (TGA) and differential thermogravimetric (DTG) data obtained under both situations have been used to evaluate the kinetic and thermodynamic parameters and elucidate the reaction mechanism. The clay has also been characterized using TGA/DTG analysis, Fourier Transform Infra-Red (FTIR) spectroscopic analysis and X-ray diffraction (XRD), Energy dispersive spectroscopy (EDS), and scanning electron microscopic (SEM) techniques. The activation energy, pre-exponential factor and thermodynamic parameters have been evaluated using the model-free iso-conversional method of Flynn-Wall-Ozawa (FWO) and Vyazovkin and the distributed activation energy model (DAEM). The Montmorillonite clay has influenced the degradation process appreciably through its catalytic action.

Genome wide characterization of the SERK/SERL gene family in Phalaenopsis equestris, Dendrobium catenatum and Apostasia shenzhenica (Orchidaceae).

Somatic embryogenesis receptor kinases (SERKs) play a significant role in morphogenesis, stress/defense and signal transduction. In the present study, we have identified two SERK and 11 SERK-like (SERL) genes in Phalaenopsis equestris, two SERK and 11 SERL genes in Dendrobium catenatum, and one SERK and eight SERL genes in Apostasia shenzhenica genome. Characterization of the SERK proteins revealed the presence of a signal peptide, a leucine zipper, five leucine-rich repeats (LRRs), a serine proline proline (SPP) motif, a transmembrane region, a kinase domain, and a C-terminus. Most of the SERK/SERL proteins were characterized with similar physicochemical properties. The presence of transmembrane region predicted their membranous localization. Tertiary structure prediction of all the five identified SERK proteins had sequence identity with BAK1 protein of Arabidopsis thaliana. Generally, all the SERK/SERL genes shared similar gene architecture and intron phasing. Gene ontology analysis indicated the role of SERKs in receptor and ATP binding, signal transduction, and protein phosphorylation. Phylogenetic analysis revealed the clustering of SERKs and SERLs in distinct clades. Expression of SERKs in reproductive tissues like floral bud, floral stalk, whole flower and pollen was reported to be higher than their expression in vegetative tissues with an exception of PeSERK1 and DcSERK1 which showed higher expression in leaves and roots, respectively. Likewise, a higher expression of AsSERK1 was observed in tubers. However, lower expression of SERLs was observed in majority of tissues studied irrespective of their vegetative or reproductive origin. This work paves way for future studies involving functional characterization of SERK/SERLs and their potential role in embryogenesis/organogenesis as an aid to regeneration and multiplication of endangered orchids.