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1.
Part Fibre Toxicol ; 17(1): 27, 2020 06 15.
Artículo en Inglés | MEDLINE | ID: mdl-32539833

RESUMEN

BACKGROUND: Wood combustion emissions have been studied previously either by in vitro or in vivo models using collected particles, yet most studies have neglected gaseous compounds. Furthermore, a more accurate and holistic view of the toxicity of aerosols can be gained with parallel in vitro and in vivo studies using direct exposure methods. Moreover, modern exposure techniques such as air-liquid interface (ALI) exposures enable better assessment of the toxicity of the applied aerosols than, for example, the previous state-of-the-art submerged cell exposure techniques. METHODS: We used three different ALI exposure systems in parallel to study the toxicological effects of spruce and pine combustion emissions in human alveolar epithelial (A549) and murine macrophage (RAW264.7) cell lines. A whole-body mouse inhalation system was also used to expose C57BL/6 J mice to aerosol emissions. Moreover, gaseous and particulate fractions were studied separately in one of the cell exposure systems. After exposure, the cells and animals were measured for various parameters of cytotoxicity, inflammation, genotoxicity, transcriptome and proteome. RESULTS: We found that diluted (1:15) exposure pine combustion emissions (PM1 mass 7.7 ± 6.5 mg m- 3, 41 mg MJ- 1) contained, on average, more PM and polycyclic aromatic hydrocarbons (PAHs) than spruce (PM1 mass 4.3 ± 5.1 mg m- 3, 26 mg MJ- 1) emissions, which instead showed a higher concentration of inorganic metals in the emission aerosol. Both A549 cells and mice exposed to these emissions showed low levels of inflammation but significantly increased genotoxicity. Gaseous emission compounds produced similar genotoxicity and a higher inflammatory response than the corresponding complete combustion emission in A549 cells. Systems biology approaches supported the findings, but we detected differing responses between in vivo and in vitro experiments. CONCLUSIONS: Comprehensive in vitro and in vivo exposure studies with emission characterization and systems biology approaches revealed further information on the effects of combustion aerosol toxicity than could be achieved with either method alone. Interestingly, in vitro and in vivo exposures showed the opposite order of the highest DNA damage. In vitro measurements also indicated that the gaseous fraction of emission aerosols may be more important in causing adverse toxicological effects. Combustion aerosols of different wood species result in mild but aerosol specific in vitro and in vivo effects.


Asunto(s)
Contaminantes Atmosféricos/toxicidad , Daño del ADN , Exposición por Inhalación/efectos adversos , Picea/química , Pinus/química , Humo/efectos adversos , Madera , Células A549 , Aerosoles , Contaminantes Atmosféricos/análisis , Animales , Técnicas de Cultivo de Célula , Supervivencia Celular/efectos de los fármacos , Citocinas/metabolismo , Calefacción , Humanos , Exposición por Inhalación/análisis , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Tamaño de la Partícula , Células RAW 264.7 , Humo/análisis , Especificidad de la Especie , Transcriptoma/efectos de los fármacos
2.
Environ Toxicol Pharmacol ; 104: 104316, 2023 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-37981204

RESUMEN

This study evaluated how exposure to the ubiquitous air pollution component, ultrafine particles (UFPs), alters the olfactory bulb (OB) transcriptome. The study utilised a whole-body inhalation chamber to simulate real-life conditions and focused on UFPs due to their high translocation and deposition ability in OBs as well as their prevalence in ambient air. Female C57BL/6J mice were exposed to clean air or to freshly generated combustion derived UFPs for two weeks, after which OBs were dissected and mRNA transcripts were investigated using RNA sequencing analysis. For the first time, transcriptomics was applied to determine changes in mRNA expression levels occurring after subacute exposure to UFPs in the OBs. We found forty-five newly described mRNAs to be involved in air pollution-induced responses, including genes involved in odorant binding, synaptic regulation, and myelination signalling pathway, providing new gene candidates for future research. This study provides new insights for the environmental science and neuroscience fields and nominates future research directions.


Asunto(s)
Contaminantes Atmosféricos , Contaminación del Aire , Ratones , Animales , Femenino , Bulbo Olfatorio/química , Bulbo Olfatorio/metabolismo , Contaminantes Atmosféricos/toxicidad , Contaminantes Atmosféricos/análisis , Transcriptoma , Ratones Endogámicos C57BL , Contaminación del Aire/análisis , Material Particulado/toxicidad , Material Particulado/análisis , Perfilación de la Expresión Génica , Biomarcadores/metabolismo , ARN Mensajero/metabolismo , Tamaño de la Partícula
3.
Neurotoxicology ; 89: 55-66, 2022 03.
Artículo en Inglés | MEDLINE | ID: mdl-34999154

RESUMEN

Epidemiological studies reveal that air pollution exposure may exacerbate neurodegeneration. Ultrafine particles (UFPs) are pollutants that remain unregulated in ambient air by environmental agencies. Due to their small size (<100 nm), UFPs have the most potential to cross the bodily barriers and thus impact the brain. However, little information exists about how UFPs affect brain function. Alzheimer's disease (AD) is the most common form of dementia, which has been linked to air pollutant exposure, yet limited information is available on the mechanistic connection between them. This study aims to decipher the effects of UFPs in the brain and periphery using the 5xFAD mouse model of AD. In our study design, AD mice and their wildtype littermates were subjected to 2-weeks inhalation exposure of UFPs in a whole-body chamber. That subacute exposure did not affect the amyloid-beta accumulation. However, when multiple cytokines were analyzed, we found increased levels of proinflammatory cytokines in the brain and periphery, with a predominant alteration of interferon-gamma in response to UFP exposure in both genotypes. Following exposure, mitochondrial superoxide dismutase was significantly upregulated only in the 5xFAD hippocampi, depicting oxidative stress induction in the exposed AD mouse group. These data demonstrate that short-term exposure to inhaled UFPs induces inflammation without affecting amyloid-beta load. This study provides a better understanding of adverse effects caused by short-term UFP exposure in the brain and periphery, also in the context of AD.


Asunto(s)
Contaminantes Atmosféricos , Material Particulado , Contaminantes Atmosféricos/toxicidad , Péptidos beta-Amiloides , Animales , Inflamación/inducido químicamente , Exposición por Inhalación/efectos adversos , Exposición por Inhalación/análisis , Ratones , Tamaño de la Partícula , Material Particulado/toxicidad
4.
Environ Toxicol Pharmacol ; 96: 104003, 2022 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-36283621

RESUMEN

Membrane transporters are important for maintaining brain homeostasis by regulating the passage of solutes into, out of, and within the brain. Growing evidence suggests neurotoxic effects of air pollution exposure and its contribution to neurodegenerative disorders, including Alzheimer's disease (AD), yet limited knowledge is available on the exact cellular impacts of exposure. This study investigates how exposure to ubiquitous solid components of air pollution, ultrafine particles (UFPs), influence brain homeostasis by affecting protein levels of membrane transporters. Membrane transporters were quantified and compared in brain cortical samples of wild-type and the 5xFAD mouse model of AD in response to subacute exposure to inhaled UFPs. The cortical ASCT1 and ABCB1 transporter levels were elevated in wild-type and 5xFAD mice subjected to a 2-week UFP exposure paradigm, suggesting impairment of brain homeostatic mechanisms. This study provides new insight on the molecular mechanisms underlying adverse effects of air pollution on the brain.


Asunto(s)
Miembro 1 de la Subfamilia B de Casetes de Unión a ATP , Contaminantes Atmosféricos , Sistema de Transporte de Aminoácidos ASC , Lóbulo Frontal , Material Particulado , Animales , Ratones , Contaminantes Atmosféricos/toxicidad , Contaminantes Atmosféricos/análisis , Tamaño de la Partícula , Material Particulado/toxicidad , Material Particulado/análisis , Sistema de Transporte de Aminoácidos ASC/análisis , Miembro 1 de la Subfamilia B de Casetes de Unión a ATP/análisis , Lóbulo Frontal/efectos de los fármacos , Lóbulo Frontal/metabolismo
5.
Neurosci Lett ; 650: 126-133, 2017 05 22.
Artículo en Inglés | MEDLINE | ID: mdl-28455100

RESUMEN

Studies of cognitive behavior in rodent models of Alzheimer's disease (AD) are the mainstay of academic and industrial efforts to find effective treatments for this disorder. However, in the majority of such studies, the nature of rodent behavioral tests is considerably different from the setting associated with cognitive assessments of individuals with AD. The recently developed touchscreen technique provides a more translational way of rodent cognitive testing because the stimulus (images in different locations on the screen) and reaction (touch) are similar to those employed in human test routines, such as the Cambridge Neuropsychological Test Automated Battery. Here, we used Visual Discrimination and Reversal of Visual Discrimination touchscreen tasks to assess cognitive performance of APPSwDI/Nos2-/- (CVN) mice, which express mutated human APP and have a homozygous deletion of the Nos2 gene. We revealed that CVN mice made more first-time errors and received more correction trials than WT mice across both discrimination and reversal phases, although mutation effect size was larger during the latter phase. These results indicate sensitivity of touchscreen-based measurements to AD-relevant mutations in CVN mice and warrant future touchscreen experiments aimed at evaluating other cognitive and motivational phenotypes in this AD mouse model.


Asunto(s)
Aprendizaje Discriminativo , Recuerdo Mental , Reconocimiento Visual de Modelos , Aprendizaje Inverso , Recompensa , Precursor de Proteína beta-Amiloide/genética , Animales , Masculino , Ratones , Ratones Noqueados , Ratones Transgénicos , Óxido Nítrico Sintasa de Tipo II/genética , Aprendizaje Inverso/fisiología , Análisis y Desempeño de Tareas
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