Compressive mechanical properties of vitrified porcine menisci are superior to frozen and similar to fresh porcine menisci.

The common practice of freezing meniscal allograft tissue is limited due to the formation of damaging ice crystals. Vitrification, which eliminates the formation of damaging ice crystals, may allow the mechanical properties of meniscal allograft tissue to be maintained during storage and long-term preservation. The primary objective of this study was to investigate the differences between fresh, frozen, and vitrified porcine lateral menisci examining compressive mechanical properties in the axial direction. Unconfined compressive stress-relaxation testing was conducted to quantify the mechanical properties of fresh, frozen and vitrified porcine lateral menisci. The compressive mechanical properties investigated were peak and equilibrium stress, secant, instantaneous and equilibrium modulus, percent stress-relaxation, and relaxation time constants from three-term Prony series. Frozen menisci exhibited inferior compressive mechanical properties in comparison with fresh menisci (significant differences in peak and equilibrium stress, and secant, instantaneous and equilibrium modulus) and vitrified menisci (significant differences in peak stress, and secant and instantaneous modulus). Interestingly, fresh and vitrified menisci exhibited comparable compressive mechanical properties (stress, modulus and relaxation parameters). These findings are significant because (1) vitrification was successful in maintaining mechanical properties at values similar to fresh menisci, (2) compressive mechanical properties of fresh menisci were characterized providing a baseline for future research, and (3) freezing affected mechanical properties confirming that freezing should be used with caution in future investigations of meniscal mechanical properties. Vitrification was superior to freezing for preserving compressive mechanical properties of menisci which is an important advance for vitrification as a preservation option for meniscal allograft transplantation.

Quantifying Asymmetry and Performance of Lower Limb Mechanical Muscle Function in Varsity Athletes-Using Non-Countermovement Jumps.

ABSTRACT: Stadnyk, M, Sepehri, M, Cook, M, Adeeb, S, and Westover, L. Quantifying asymmetry and performance of lower limb mechanical muscle function in varsity athletes-using non-countermovement jumps. J Strength Cond Res 37(1): 98-106, 2023-The ability to automatically quantify jump performance and lower limb muscle function in athletes would be beneficial for both training and rehabilitation purposes. Countermovement jumps (CMJs) and non-CMJs (NCMJs) are simple, quick, and require relatively inexpensive equipment to effectively and reliably monitor lower limb function. In a previous study, CMJ characteristics were assessed across different varsity sports. This study is a follow-up study to incorporate NCMJs into assessing jump characteristics of the same sports and investigate the additional information provided by the complementary jump type. The main objective of this study was to look at a means of quantifying the lower limb mechanical muscle function automatically to provide information for rehabilitation and performance purposes in athletes of specific sports. Male and female varsity athletes from 4 different sports completed 5 trials of an NCMJ on dual force plates. An analysis program was developed using Wolfram Mathematica to analyze force-time jump data. Various parameters of interest were generated, including peak force, force-time curve shape classification, jump phase lengths, phase-specific kinetic impulse, asymmetry index, takeoff velocity, jump height, phase-specific center of mass displacements, and reactive strength index modified. Results obtained indicate that similar jump characteristics to the CMJ study can be quantified, which can be used for performance enhancement or injury rehabilitation. Additional data found, such as the ability of an athlete to hold a steady squat during an NCMJ, could also be meaningful in aiding trainers to design programs tailored for athletes.

Modelling and evaluating periodontal ligament mechanical behaviour and properties: A scoping review of current approaches and limitations.

This scoping review is intended to synthesize the techniques proposed to model the tooth-periodontal ligament-bone complex (TPBC), while also evaluating the suggested periodontal ligament (PDL) material properties. It is concentrated on the recent advancements on the PDL and TPBC models, while identifying the advantages and limitations of the proposed approaches. Systematic searches were conducted up to December 2020 for articles that proposed PDL models to assess orthodontic tooth movement in Compendex, Web of Science, EMBASE, MEDLINE, PubMed, ScienceDirect, Google Scholar and Scopus databases. Although there have been many studies focused on the evaluation of PDL material properties through numerous modelling approaches, only a handful of approaches have been identified to investigate the interface properties of the PDL as a complete dynamical system (TPBC models). Past reviews on the analytical and experimental determination of the PDL properties already show a concerning range in reported output values-some nearly six orders of magnitude in difference-that strongly suggested the need for further investigation. Surprisingly, it has not yet been possible to determine a narrower range of values for the PDL material properties. Moreover, very few scientific approaches address the TPBC as an integrated complex system model. In consequence, current methods for capturing the PDL material behaviour in a clinical setting are limited and inconclusive. This synthesis encourages more systematic, pragmatic and phenomenological research in this area.

An Investigation of the Effect of Irradiation on the Biomechanical Properties of Fibular Grafts.

PURPOSE: Irradiation of fibular grafts for jaw reconstruction following mandibulectomy can be associated with increased graft failure and implant instability. The objective of this study is to investigate the effect of isolated irradiation on the biomechanical properties of ex-vivo porcine fibula grafts. It was hypothesized that the isolated irradiation (without biological response) will not significantly influence the biomechanical properties of the fibular grafts. METHODS: Forty porcine fibular grafts (n = 20 irradiated and n = 20 nonirradiated) were obtained. The irradiated group was subjected to a single dose of 60 Gy of irradiation with cesium 137. Both groups underwent 3-point bending tests with cyclic loading for 1500 cycles (50 to 500 N at 2 Hz) followed by push to failure. Rate of damage (mm/s), stiffness (N/mm), modulus of elasticity (MPa), maximum load (N), maximum displacement (mm), and maximum stress (MPa) were compared between the 2 groups using independent t tests (P < .05). RESULTS: No significant differences were found between the irradiated and nonirradiated groups for any of the biomechanical parameters (all P values > 0.05). CONCLUSIONS: The results of this study support our hypothesis that even high levels of isolated irradiation do not significantly affect the biomechanical properties of fibular bone grafts. This work allows us to exclude acute structural changes in the bone due to irradiation as a possible factor leading to bone/implant instability following jaw reconstruction with fibular grafts. Further studies need to be conducted to better understand the range of factors that may lead to implant instability, including the biological response after radiation therapy.

An Equivalent Constitutive Model of Cancellous Bone With Fracture Prediction.

To simulate the mechanical and fracture behaviors of cancellous bone in three anatomical directions and to develop an equivalent constitutive model. Microscale extended finite element method (XFEM) models of a cancellous specimen were developed with mechanical behaviors in three anatomical directions. An appropriate abaqus macroscale model replicated the behavior observed in the microscale models. The parameters were defined based on the intermediate bone material properties in the anatomical directions and assigned to an equivalent nonporous specimen of the same size. The equivalent model capability was analyzed by comparing the micro- and macromodels. The hysteresis graphs of the microscale model show that the modulus is the same in loading and unloading; similar to the metal plasticity models. The strength and failure strains in each anatomical direction are higher in compression than in tension. The microscale models exhibited an orthotropic behavior. Appropriate parameters of the cast iron plasticity model were chosen to generate macroscale models that are capable of replicating the observed microscale behavior of cancellous bone. Cancellous bone is an orthotropic material that can be simulated using a cast iron plasticity model. This model is capable of replicating the microscale behavior in finite element (FE) analysis simulations without the need for individual trabecula, leading to a reduction in computational resources without sacrificing model accuracy. Also, XFEM of cancellous bone compared to traditional finite element method proves to be a valuable tool to predict and model the fractures in the bone specimen.

Evaluation of word recognition and word recall with bone conduction devices: do directional microphones free up cognitive resources?

Objective: To determine if directional microphones improve cognitive capacity in typically hearing adults. The study objectives are to evaluate differences in (1) speech recognition and (2) working memory through a word recall task between bilateral directional and omnidirectional microphone settings.Design: A conductive hearing loss was artificially induced while participants wore bilateral bone conduction hearing aids on softbands. For each hearing aid setting (bilateral omnidirectional and bilateral directional), seven blocks of seven sentences from the Hearing in Noise Test (HINT) were presented at a signal-to-noise ratio of +2 dB. Participants repeated each sentence aloud and after each block, wrote down as many of the last words as they could recall.Study sample: Thirty-five typical hearing adults and a subset (n = 20) achieving ≥80% recognition.Results: The directional microphone setting showed significant improvement over the omnidirectional setting for recognition and recall for both the full set of participants and the subset of participants with ≥ 80% recognition.Conclusions: This study demonstrated that features such as directional microphones can improve both speech recognition and working memory. Even in listening situations where participants can understand the majority of speech, directional microphones may offer improvements to cognitive capacity and reduce listening effort.

Development of a Novel Bone Conduction Verification Tool Using a Surface Microphone: Validation With Percutaneous Bone Conduction Users.

OBJECTIVES: To determine if a newly-designed, forehead-mounted surface microphone would yield equivalent estimates of audibility when compared to audibility measured with a skull simulator for adult bone conduction users. DESIGN: Data was analyzed using a within subjects, repeated measures design. There were two different sensors (skull simulator and surface microphone) measuring the same hearing aid programmed to the same settings for all subjects. We were looking for equivalent results. PATIENTS: Twenty-one adult percutaneous bone conduction users (12 females and 9 males) were recruited for this study. Mean age was 54.32 years with a standard deviation of 14.51 years. Nineteen of the subjects had conductive/mixed hearing loss and two had single-sided deafness. METHODS: To define audibility, we needed to establish two things: (1) in situ-level thresholds at each audiometric frequency in force (skull simulator) and in sound pressure level (SPL; surface microphone). Next, we measured the responses of the preprogrammed test device in force on the skull simulator and in SPL on the surface mic in response to pink noise at three input levels: 55, 65, and 75 dB SPL. The skull simulator responses were converted to real head force responses by means of an individual real head to coupler difference transform. Subtracting the real head force level thresholds from the real head force output of the test aid yielded the audibility for each audiometric frequency for the skull simulator. Subtracting the SPL thresholds from the surface microphone from the SPL output of the test aid yielded the audibility for each audiometric frequency for the surface microphone. The surface microphone was removed and retested to establish the test-retest reliability of the tool. RESULTS: We ran a 2 (sensor) × 3 (input level) × 10 (frequency) mixed analysis of variance to determine if there were any significant main effects and interactions. There was a significant three-way interaction, so we proceeded to explore our planned comparisons. There were 90 planned comparisons of interest, three at each frequency (3 × 10) for the three input levels (30 × 3). Therefore, to minimize a type 1 error associated with multiple comparisons, we adjusted alpha using the Holm-Bonferroni method. There were five comparisons that yielded significant differences between the skull simulator and surface microphone (test and retest) in the estimation of audibility. However, the mean difference in these effects was small at 3.3 dB. Both sensors yielded equivalent results for the majority of comparisons. CONCLUSIONS: Models of bone conduction devices that have intact skin cannot be measured with the skull simulator. This study is the first to present and evaluate a new tool for bone conduction verification. The surface microphone is capable of yielding equivalent audibility measurements as the skull simulator for percutaneous bone conduction users at multiple input levels. This device holds potential for measuring other bone conduction devices (Sentio, BoneBridge, Attract, Soft headband devices) that do not have a percutaneous implant.

Comparison of implant stability measurement devices for bone-anchored hearing aid systems.

STATEMENT OF PROBLEM: The success of implants for bone-anchored hearing aids (BAHA) relies on proper osseointegration at the bone-implant interface. Clinical evaluation of implant stability is important in prescribing loading, identifying the risk of failure, and monitoring the long-term health of the implant. PURPOSE: The purpose of this in vitro study was to evaluate 2 measurement systems for BAHA implant stability: the most commonly used, Osstell implant stability quotient (ISQ), and a newly developed advance system for implant stability testing (ASIST). MATERIAL AND METHODS: BAHA implants (Oticon Medical Ponto and Cochlear BAHA Connect systems) were installed in plastic materials with adhesive to simulate implants integrated in bone with varying levels of interface stiffness. Different lengths of BAHA abutments were used with each implant specimen, and stability measurements were obtained with both the Osstell ISQ and the ASIST systems. The measurement systems were evaluated in terms of sensitivity to differences in interface stiffness and the effect of abutment length on the stability measurement. Repeated measures ANOVA followed by post hoc t tests were used for the comparisons with a Bonferroni adjusted alpha value of .05/15 = .003 to control for potential type 1 errors. RESULTS: Changing the abutment length of a single implant installation had minimal effect on the ASIST stability coefficient, whereas large variations were observed in the Osstell implant stability quotient (ISQ). The Osstell showed a clear relationship of decreasing ISQ with increasing abutment length for both the Oticon Medical and the Cochlear implant systems. Both the ASIST and the Osstell were found to be sensitive to changes in interface properties, with the ASIST being more sensitive to these changes. CONCLUSIONS: The ASIST system is more sensitive to changes in interface properties and shows smaller variation because of changes in abutment length than the Osstell ISQ system.

An analytical model to measure dental implant stability with the Advanced System for Implant Stability Testing (ASIST).

A non-invasive method of quantitatively assessing dental implant stability is important to monitor its long-term health. The Advanced System for Implant Stability Testing (ASIST) is a noninvasive technique that couples the impact technique with a linear vibration model of the implant system, such that the measured signal can be used to determine a matching analytical response. The purpose of this study was to evaluate the ASIST technique by comparing stability estimates obtained from artificial implant installations with various abutments. Two Straumann dental implants were installed in four densities of uniform polyurethane foam, and the stability of each installation was measured using different healing abutments and artificial dental crowns. With the ASIST, values for the estimated interfacial stiffness increased with foam density and did not significantly change with abutment type for a specific sample. This provides evidence that the analytical model is representative of the physical system. Current methods, such as resonance frequency analysis, interpret the interface stiffness based on a single frequency measurement. With the ASIST, the measured signal provides information about the first and second modes of vibration of the implant system, both of which are influenced by the properties of the corresponding abutment. The consideration of both modes allows the technique to reliably measure the interfacial stiffness independently of the system components. As a result, the ASIST technique may provide an improved non-invasive method of measuring the stability of dental implants.

Evaluation of a vibration modeling technique for the in-vitro measurement of dental implant stability.

The Advanced System for Implant Stability Testing (ASIST) is a device currently being developed to noninvasively measure implant stability by estimating the mechanical stiffness of the bone-implant interface, which is reported as the ASIST Stability Coefficient (ASC). This study's purpose was to determine whether changes in density, bonding, and drilling technique affect the measured vibration of a dental implant, and whether they can be quantified as a change in the estimated BII stiffness. Stability was also measured using RFA, insertion torque (IT) and the pullout test. Bone-level tapered implants (4.1 mm diameter, 10 mm length) were inserted in polyurethane foam as an artificial bone substitute. Samples were prepared using different bone densities (20, 30, 40 PCF), drilling sequences, and superglue to simulate a bonded implant. Measurements were compared across groups at a significance level of 0.05. The ASC was able to indicate changes in each factor as a change in the interfacial stiffness. IT and pullout force values also showed comparable increases. Furthermore, the relative difference in ISQ values between experimental groups was considerably smaller than the ASC. While future work should be done using biological bone and in-vivo systems, the results of this in-vitro study suggest that modelling of the implant system with a vibration-based approach may provide a noninvasive method of assessing the mechanical stability of the implant.

Algorithmically designed flaps in tongue reconstruction: a feasibility analysis.

PURPOSE: Despite the significance and complexity of tongue reconstruction surgery, a digital tool for flap design is currently lacking. This study investigates the effectiveness of employing inverse finite element method (IFEM) for meticulously designing the geometric characteristics of harvested tissue (free flap) for tongue reconstruction. METHODS: In the case of an artificially simulated hemiglossectomy, IFEM algorithm was applied for algorithmic flap design. The method's effectiveness was evaluated by assessing flap deformation in a simplified virtual reconstruction, focusing on parameters such as stress, strain, and thickness. RESULTS: The IFEM algorithm successfully generated an optimal flap design for the intended surgical removal. Analysis of the flap's overall surface area, deformation characteristics, and safety margins demonstrated the feasibility of the deformation. Notably, the stress and thickness assessments suggested that the flap's tension post-surgery would not adversely affect the mobility of the reconstructed tongue, suggesting a positive outcome for functional recovery. CONCLUSION: The IFEM demonstrates significant potential as a tool for precise free flap design in tongue reconstruction surgeries. Its application could lead to improved surgical accuracy and better quality of life for patients undergoing such procedures.

Effect of adding Schroth physiotherapeutic scoliosis specific exercises to standard care in adolescents with idiopathic scoliosis on posture assessed using surface topography: A secondary analysis of a Randomized Controlled Trial (RCT).

BACKGROUND: Adolescent idiopathic scoliosis (AIS) is a three-dimensional structural asymmetry of the spine and trunk affecting 2-4% of adolescents. Standard treatment is observation, bracing, and surgery for small, moderate, and large curves, respectively. Schroth exercises aim to correct posture and reduce curve progression. PURPOSE: This study aimed to determine the effect of Schroth exercises added to the standard care compared to standard care alone on torso asymmetry in AIS. METHODS: In a randomized controlled trial (NCT01610908), 124 participants with AIS (age: 10-18, Cobb: 10°-45°, Risser: ≤3) were randomly assigned to the control (Standard care only) or Schroth (Standard care + Schroth treatment) group. Schroth treatment consisted of 1-hour weekly supervised sessions and 30-45 minutes of daily home exercises for six months. The control group received Schroth exercises in the last six months of the 1-year monitoring period. Markerless 3D surface topography assessed torso asymmetry measured by maximum deviation (MaxDev) and root mean square (RMS). Intention to treat linear mixed effects model analysis was compared to the per protocol analysis. RESULTS: In the intention to treat analysis, the Schroth group (n = 63) had significantly larger decreased RMS (-1.2 mm, 95%CI [-1.5,-0.9]mm, p = 0.012) and MaxDev (-1.9mm, 95%CI [-2.4,-1.5]mm, p = 0.025) measurements compared to controls (n = 57) after six months of intervention. In the per protocol analysis (Schroth n = 39, control n = 36), the Schroth group also had a significantly larger decrease compared to the control in both the RMS (-1.0mm, 95%CI [-1.9, -0.2]mm, p = 0.013) and MaxDev measurements (-2.0mm, 95%CI [-3.3,-0.5]mm, p = 0.037). For the control group, both the intention to treat and per protocol analysis showed no difference in RMS and MaxDev in the last six months of Schroth intervention (p>0.5). CONCLUSION: Schroth Exercise treatment added to standard care (observation or bracing) reduced asymmetry measurements in AIS. As expected, a greater effect was observed for participants who followed the prescribed exercise treatment per protocol.

Inverse finite element analysis for an axisymmetric model of vertical tooth extraction.

BACKGROUND AND OBJECTIVE: Tooth extraction is a common clinical procedure with biomechanical factors that can directly influence patient outcomes. Recent development in atraumatic extraction techniques have endeavoured to improve treatment outcomes, but the characterization of extraction biomechanics is sparse. An axisymmetric inverse finite element (FE) approach is presented to represent the biomechanics of vertical atraumatic tooth extraction in an ex-vivo swine model. METHODS: Geometry and boundary conditions from the model are determined to match the extraction of swine incisors in a self-aligning ex vivo extraction experiment. Material parameters for the periodontal ligament (PDL) model are determined by solving an inverse FE problem using clusters of data obtained from 10 highly-controlled mechanical experiments. A seven-parameter visco-hyperelastic damage model, based on an Arruda-Boyce framework, is used for curve fitting. Three loading schemes were fit to obtain a common set of material parameters. RESULTS: The inverse FE results demonstrate good predictions for overall force-time curve shape, peak force, and time to peak force. The fit model parameters are sufficiently consistent across all three cases that a coefficient-averaged model was taken that compares well to all three cases. Notably, the initial modulus ,u, converged across trials to an average value of 0.472 MPa with an average viscoelastic constant g of 0.561. CONCLUSIONS: The presented model is found to have consistent parameters across loading cases. The capability of this model to represent the fundamental mechanical characteristics of the dental complex during vertical extraction loading is a significant advancement in the modelling of extraction procedures. Future work will focus on verifying the model as a predictive design tool for assessing new loading schemes in addition to investigating its applications to subject-specific problems.

Biomechanical Comparison of Distal Radioulnar Joint Reconstruction Graft Preparation Techniques.

Background Graft preparation techniques for the Adams-Berger distal radioulnar joint (DRUJ) reconstruction vary among surgeons with insufficient evidence to support any specific technique. Questions/Purposes We compared survival with cyclic loading, absolute elongation, elongation rate, and modes of failure of four graft preparation techniques. Methods Fifteen porcine extensor tendons were divided into three equal groups: tendon only; tendon augmented along its full length with nonlocking 2-0 FiberLoop suture spaced at 6 mm intervals; and tendon with suture at 12 mm intervals. Suture only was also tested. Samples were woven through custom radius- and ulna-simulating jigs mounted on a mechanical testing machine. Samples underwent a staircase cyclic loading protocol and were then inspected visually for the mode of failure. Survival with cyclic loading, absolute elongation, and elongation rate was compared. Results Average survival with cyclic loading of suture-augmented tendon was significantly higher than tendon only. All tendon groups had significantly higher survival compared with suture only. Absolute elongation was subject to variability due to initial nonlinear elongation behavior of samples. The elongation rate was significantly lower with suture compared with all tendon groups. Modes of failure included rupture of the tendon and/or suture at the simulated graft-bone interface and elongation of the entire construct without rupture. Conclusions In this biomechanical study, augmentation of porcine tendons with suture spaced at either 6 or 12 mm for DRUJ reconstruction significantly increased survival to a staircase cyclic loading protocol Clinical Relevance For the Adams-Berger reconstruction, tendon grafts augmented along their entire length by nonabsorbable braided suture are biomechanically superior to tendon alone.

The effect of simulated radiation induced fibrosis on tongue protrusion.

Radiation therapy (RT) is an important adjuvant and primary treatment modality for head and neck cancers. A severe side effect of RT is fibrosis or scarring of muscle tissues of the oral cavity including the tongue. Previous studies have demonstrated that increased radiation doses to the oral cavity structures have led to decrements in function, hypothesized to result from changes in muscle tissue properties that affect the tongue's function. To understand the complex relationship between tongue muscle fibrosis and tongue function, the current study used a virtual biomechanical model of the tongue. Fibrosis parameters including density (high, low), area (large, small) and location (946 node centres) were systematically varied in the model to test its impact on a target tongue tip motion (protrusion). The impact of fibrosis lesion parameters on three directional components of the tip (anterior-inferior, lateral-medial, and superior-inferior) were analyzed using multi linear regression models. Increases in density and area of fibrosis significantly predicted tongue protrusion movements compared to baseline. In the anterior-posterior direction, reductions in the tongue protrusion were observed. In the inferior-superior direction, the tongue height remained above baseline for the majority of cases. In the lateral-medial direction, ipsilateral deviations were observed. The location of fibrosis modulated these three main effects by either amplifying the observed effect or minimizing it. The findings support the hypothesis that changes in muscle tissue properties because of fibrosis impact tongue function. Increases in density and area of fibrosis impact key muscles in the target motion. The range of modulating effects of the lesion location (i.e., either amplifying or minimizing certain impact patterns) highlights the intricacy of tongue anatomy/soft tissue biomechanics and may suggest that lesions in any location will compromise the tongue's movement.

Development and validation of a novel ankle joint musculoskeletal model.

An improved understanding of contact mechanics in the ankle joint is paramount for implant design and ankle disorder treatment. However, existing models generally simplify the ankle joint as a revolute joint that cannot predict contact characteristics. The current study aimed to develop a novel musculoskeletal ankle joint model that can predict contact in the ankle joint, together with muscle and joint reaction forces. We modelled the ankle joint as a multi-axial joint and simulated contact mechanics between the tibia, fibula and talus bones in OpenSim. The developed model was validated with results from experimental studies through passive stiffness and contact. Through this, we found a similar ankle moment-rotation relationship and contact pattern between our study and experimental studies. Next, the musculoskeletal ankle joint model was incorporated into a lower body model to simulate gait. The ankle joint contact characteristics, kinematics, and muscle forces were predicted and compared to the literature. Our results revealed a comparable peak contact force and the same muscle activation patterns in four major muscles. Good agreement was also found in ankle dorsi/plantar-flexion and inversion/eversion. Thus, the developed model was able to accurately model the ankle joint and can be used to predict contact characteristics in gait.

Jumping into recovery: A systematic review and meta-analysis of discriminatory and responsive force plate parameters in individuals following anterior cruciate ligament reconstruction during countermovement and drop jumps.

Purpose: Comprehensive understanding of force plate parameters distinguishing individuals postprimary anterior cruciate ligament reconstruction (ACLR) from healthy controls during countermovement jumps (CMJ) and/or drop jumps (DJ) is lacking. This review addresses this gap by identifying discriminative force plate parameters and examining changes over time in individuals post-ACLR during CMJ and/or DJ. Methods: We conducted a systematic review and meta analyses following the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) guidelines. Nine databases were searched from inception to March 2022. We included cross-sectional papers comparing post-ACLR with healthy controls or longitudinal studies of individuals at least 6 months postprimary ACLR while performing CMJ and/or DJ on force plates. The methodological quality was appraised using the Modified Downs and Black Checklist. Results: Thirty-three studies including 1185 (50.38%) participants post-ACLR, and 1167 (49.62%) healthy controls, were included. Data were categorised into single-leg CMJ, double-leg CMJ, single-leg DJ, and double-leg DJ. Jump height was reduced in both single (mean difference [MD] = -3.13; p < 0.01; 95% confidence interval [CI]: [-4.12, -2.15]) and double-leg (MD = -4.24; p < 0.01; 95% CI: [-5.14, -3.34]) CMJs amongst individuals with ACLR. Similarly, concentric impulse and eccentric/concentric impulse asymmetry could distinguish between ACLR (MD = 3.42; p < 0.01; 95% CI: [2.19, 4.64]) and non-ACLR (MD = 5.82; p < 0.01; 95% CI: [4.80, 6.80]) individuals. In double-leg DJs, peak vertical ground reaction forces were lower in the involved side (MD = -0.10; p = 0.03; 95% CI: [-0.18, -0.01]) but higher in the uninvolved side (MD = 0.15; p < 0.01; 95% CI: [0.10, 0.20]) when compared to controls and demonstrated significant changes between 6 months and 3 years post-ACLR. Conclusion: This study identified discriminative kinetic parameters when comparing individuals with and without ACLR and also monitored neuromuscular function post-ACLR. Due to heterogeneity, a combination of parameters may be required to better identify functional deficits post-ACLR. Level of Evidence: Level III.

Evaluation of the Transfemoral Bone-Implant Interface Properties Using Vibration Analysis.

Evaluating the bone-implant interface (BII) properties of osseointegrated transfemoral (TFA) implants is important for early failure detection and prescribing loads during rehabilitation. The objective of this work is to derive and validate a 1D finite element (FE) model of the Osseointegrated Prosthetic Limb (OPL) TFA system that can: (1) model its dynamic behaviour and (2) extract the BII properties. The model was validated by: (1) comparing the 1D FE formulation to the analytical and 3D FE solutions for a simplified cylinder, (2) comparing the vibration modes of the actual TFA geometry using 1D and 3D FE models, and (3) evaluating the BII properties for three extreme conditions (LOW, INTERMEDIATE, and HIGH) generated using 3D FE and experimental (where the implant was embedded, using different adhesives, in synthetic femurs) signals for additional validation. The modes predicted by the 1D FE model converged to the analytical and the 3D FE solutions for the cylinder. The 1D model also matched the 3D FE solution with a maximum frequency difference of 2.02% for the TFA geometry. Finally, the 1D model extracted the BII stiffness and the system's damping properties for the three conditions generated using the 3D FE simulations and the experimental INTERMEDIATE and HIGH signals. The agreement between the 1D FE and the 3D FE solutions for the TFA geometry indicates that the 1D model captures the system's dynamic behaviour. Distinguishing between the different BII conditions demonstrates the 1D model's potential use for the non-invasive clinical evaluation of the TFA BII properties.

A novel method for simulating ex vivo tooth extractions under varying applied loads.

BACKGROUND: Tooth extraction is a common surgical procedure where the invasiveness of the surgery can affect the nature of the dentoalveolar remodelling which follows. However, there is very little biomechanical data relating the loading applied during tooth extraction to the outcomes of the procedure. The purpose of this pilot study is to present a novel ex vivo experimental method for measuring tooth extraction mechanics and to explore preliminary metrics for predicting extraction success. METHODS: A custom experimental apparatus was developed in-house to extract central incisors from ex vivo swine mandible samples. Twenty-five (n = 25) incisors were extracted at different rates in displacement- and force-control, along with an intermittent ramp-hold scheme for a total of five schemes. Peak forces and extraction success were recorded for each test. Video analysis assisted in determining the instantaneous stiffnesses of the dental complex during continuous extractions, which were compared using the K-means clustering algorithm. FINDINGS: Tooth extraction forces ranged from 102 N to 309 N, with higher-rate tests tending towards higher peak forces (141 N - 308 N) than the lower-rate tests (102 N-204 N) for displacement- and force-controlled schemes. The K-means algorithm clearly identified load rates among tests, indicating that higher-rate loading increased system stiffness relative to the lower-rate tests. INTERPRETATION: The developed experimental method demonstrated a desirable degree of control. The preliminary results suggest the influence of load rate on the mechanical response of the dental complex and extraction outcome. Future work will further investigate the biomechanics of tooth extraction and relate them to tissue damage to improve future tooth extraction procedures.

Mechanical Properties of Fresh, Frozen and Vitrified Articular Cartilage.

Osteochondral allograft transplantations are typically used to treat focal articular cartilage injuries where the damaged cartilage is replaced with fresh cadaveric donor grafts. Despite the notable success rate of this procedure, it is limited by fresh donor tissue availability which can only be stored for approximately 28 days after harvest. Vitrification, a form of cryopreservation, can extend the storage time of cartilage. Although it has shown to preserve chondrocyte viability, its effect on the mechanical properties of the tissue has not been thoroughly investigated. Therefore, in this study, the mechanical properties of fresh, frozen, and vitrified articular cartilage were evaluated through unconfined compression testing. Results showed that the peak modulus, equilibrium modulus, and relaxation time constants of the vitrified and control samples (tested one day after harvest) were similar and higher than the fresh (tested 21 days after harvest) and frozen samples. This demonstrated that vitrification does not adversely affect the mechanical properties of cartilage and can be used as an alternative to fresh allografts which are limited by storage time. The fresh samples also had inferior mechanical properties compared to the control samples suggesting that vitrified allografts could potentially improve clinical outcomes in addition to increasing donor tissue availability.