RESUMEN
OBJECTIVE: To investigate effects of anti-dsDNA autoantibodies on growth of tumor in vitro and in vivo. METHODS: BALB/c mice were inoculated with inactivated tumor cells and challenged s.c. with SP 2/0 and Wehi 164 tumor cells four weeks after the last inoculation. The naïve mice were inoculated with SP 2/0 tumor cells immediately after incubating with sera derived from the immunized mice at week 6. Then the tumor size was examined. In vitro, the cytotoxicity of anti-dsDNA autoantibodies to tumor cells was analysed. Furthermore, apoptosis of SP 2/0 and Wehi 164 tumor cells induced by anti-dsDNA autoantibodies was examined by FACS. RESULTS: In vivo study showed that the growth of SP 2/0 and Wehi 164 tumors were inhibited in mice with anti-dsDNA autoantibodies, but not in mice lack of anti-dsDNA autoantibodies. In vitro, apoptosis of SP 2/0 and Wehi 164 tumor cells was induced when the tumor cells were incubated with the sera containing anti-dsDNA autoantibodies. Statistical analysis showed that the ability of anti-dsDNA autoantibodies to induce apoptosis of SP 2/0 and Wehi 164 tumor cells was significantly correlated with affinity (r = 0.990, P < 0.01 and r = 0.901, P < 0.05). CONCLUSION: Anti-dsDNA autoantibodies have inhibitory effect on tumor cells via inducing apoptosis.
Asunto(s)
Anticuerpos Antineoplásicos/inmunología , Apoptosis , Autoanticuerpos/inmunología , ADN/inmunología , Mieloma Múltiple/prevención & control , Animales , Anticuerpos Antineoplásicos/biosíntesis , Autoanticuerpos/biosíntesis , Línea Celular Tumoral , Fibrosarcoma/patología , Fibrosarcoma/prevención & control , Sueros Inmunes/inmunología , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos DBA , Mieloma Múltiple/patología , Trasplante de NeoplasiasRESUMEN
By means of utilizing sunflower oil and Jatropha oil as raw oil respectively, the biodiesel transesterification production and the multi-stage extracting separation were carried out experimentally. Results indicate that dealcoholized crude glycerol can be utilized as the extracting agent to achieve effective separation of methanol from the methyl ester phase, and the glycerol content in the dealcoholized methyl esters is as low as 0.02 wt.%. For the biodiesel separation process utilizing glycerol extracting dealcoholization, its technical and equipment information were acquired through the rigorous process simulation in contrast to the traditional biodiesel distillation separation process, and results show that its energy consumption decrease about 35% in contrast to that of the distillation separation process. The glycerol extracting dealcoholization has sufficient feasibility and superiority for the biodiesel separation process.