Responses of keratinocytes to Trichophyton mentagrophyte infection based on whole transcriptome analysis.

BACKGROUND: Dermatophytosis is an intractable superficial mycosis in humans and animals mainly caused by Trichophyton mentagrophytes (T. mentagrophytes), with a global prevalence of about 20%. Keratinocytes are the most abundant participants in skin immunity, and they also play a role in the first-line defence against T. mentagrophytes. However, no studies of keratinocyte responses against T. mentagrophytes infection based on the whole transcriptome have been reported. OBJECTIVES: Here, we systematically analysed changes in keratinocytes infected with T. mentagrophytes using whole transcriptome sequencing technology. METHODS: The phenotypic changes in keratinocytes after infection with 1 × 105 conidia/mL T. mentagrophytes were observed by light microscopy, scanning electron microscopy, transmission electron microscopy and terminal deoxynucleotidyl transferase dUTP nick end labeling. RNA-sequencing (RNA-seq), small RNA-seq technology and related bioinformatics methods were used to systematically analyse the whole transcriptome changes in keratinocytes upon T. mentagrophytes stimulation. RESULTS: We found that T. mentagrophytes infection caused morphological changes, membrane damage, the formation of irregular organelles and keratinocyte apoptosis. A total of 204 differentially expressed (DE) circular RNAs (circRNAs), 868 DE long noncoding RNAs (lncRNAs), 2973 DE mRNAs and 209 DE micro RNAs (miRNAs) were identified between noninfected and T. mentagrophytes-infected keratinocytes. The expression level of selected RNAs was validated by quantitative real-time polymerase chain reaction (qRT-PCR). Functional enrichment analysis revealed that the parental genes of DE circRNAs were related to cell response, cell death and establishment of the skin barrier. Genes targeted by miRNA were involved in regulating the initiation of the immune response. Based on the expression level of circRNAs, lncRNAs, mRNAs and miRNAs, circRNA-miRNA-mRNA competing endogenous (ceRNA) networks comprised of 159 DE miRNAs, 141 DE circRNAs and 2307 DE mRNAs, and lncRNA-miRNA-mRNA ceRNA networks comprised of 790 DE lncRNAs, 190 DE miRNAs and 2663 DE mRNAs were constructed. The reliability of two selected ceRNA networks was verified using qRT-PCR. Further functional enrichment analysis revealed that the DE mRNAs interacting with circRNAs and lncRNAs in the ceRNA network mainly participated in fungal recognition, inflammation, the innate immune response and the death of keratinocytes. CONCLUSIONS: Our findings might provide new evidence on the pathogenesis of T. mentagrophytes-induced dermatophytosis, which is essential for identifying new therapeutic targets for dermatophytosis treatment.

The impacts of international trade on global greenhouse gas emissions: A thought experiment based on a novel no-trade analysis.

Trade has been substantially influencing regional economic development, environmental sustainability, and human well-being. Enabled by the decomposition analysis, pollution haven hypothesis or "no-trade" scenarios (NTSs), the effects of trade on global/national social-economic-environmental development have been revealed. However, major limitations (e.g., using with-trade economic structures or neglecting price differences) existed in previous studies, and thus made the previous assessments of trade's effects unsatisfactorily. This study develops a novel NTS that addresses the existing limitations, and further applies it to estimate the effect of trade on global economic development and greenhouse gas (GHG) emissions. We show that current international trade benefits the global economic growth but with a consequence of more GHG emissions compared with the NTS. The hypothetical production in small countries (e.g., Luxembourg or Japan) would be more constrained by the production factors (e.g., land) under the NTS, compared with those factor-endowment countries (e.g., the United States or India). For country-specific analysis, we find that today's developed countries would have a substantial increase in their GHG emissions of clothing- and service-related products under the NTS, whereas countries with net-export (e.g., China or Brazil) would have less GHG emissions under the NTS. Enhancing future global collaborations is vital, especially for small or resource-deficient economies, if they are to achieve the Sustainable Development Goals.

Fabrication of dual physically cross-linked polyvinyl alcohol/agar hydrogels with mechanical stability and antibacterial activity for wound healing.

Bacterial infection is one of the most critical obstacles in wound healing, and severe bacterial infections can lead to inflammatory conditions and delay the healing process. Herein, a novel hydrogel based on polyvinyl alcohol (PVA), agar, and silk-AgNPs was prepared using a straightforward one-pot physical cross-linking method. The in situ synthesis of AgNPs in hydrogels exploited the reducibility of tyrosine (Tyr tyrosine) in silk fibroin, which endowed the hydrogels with outstanding antibacterial qualities. In addition, the strong hydrogen bond cross-linked networks of agar and the crystallites formed by PVA as the physical cross-linked double network of the hydrogel gave it excellent mechanical stability. The PVA/agar/SF-AgNPs (PASA) hydrogels exhibited excellent water absorption, porosity, and significant antibacterial effects against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Furthermore, in vivo experimental results confirmed that the PASA hydrogel significantly promoted wound repair and skin tissue reconstruction by reducing inflammation and promoting collagen deposition. Immunofluorescence staining showed that the PASA hydrogel enhanced CD31 expression to promote angiogenesis while decreasing CD68 expression to reduce inflammation. Overall, the novel PASA hydrogel showed great potential for bacterial infection wound management.

Integrating the inter- and intra-annual dynamic features of capital into environmental footprint assessment: Revisiting China's greenhouse gas footprints, 1995-2015.

Assessments of the displacement of pollution emissions or other resources through trade attribute local production to remote consumers, yielding environmental footprints for the respective regions. Recently, the previously neglected temporal dimension of capital goods-built up in the past and continuously serving production activities in the future-has received increasing attention in environmental footprint assessments. Based on an inter-annual dynamic capital-endogenised multi-regional input-output model, this study further integrates the intra-annual dynamic features of capital production and consumption. We quantify the greenhouse gas (GHG) emissions embodied in China's capital consumption over the past two decades and assign this part of GHG emissions into finished goods and services over time. Our results show that China's GHG footprint in 2015 would be 7 Gt if capital-related GHG emissions were considered. This figure is 28% higher than the GHG footprints of China's final consumption estimated by conventional consumption-based accounting (CBA) and 41% lower than that of China's final demand estimated by conventional CBA. Overall, around 8% of GHG footprints would be overestimated if we do not consider the intra-annual dynamic feature of capital in the assessment. The inter-annual allocation shows that the capital-related GHGs of China's final consumption emitted on average in the last six years, which is still increasing owing to the long lifespans of capital assets. In light of the synergies among capital development, environmental sustainability, and human needs satisfaction, it is vital to uphold economic and environmental efficiency in capital development decision-making.