[Primary culture of preadipocytes from human adults].

OBJECTIVE: To establish a method for primary culture of human preadipocyte as the seed cells for adipose tissue engineering. METHOSA: Fibroblast-like cells were cultured from adult human abdominal adipose tissue. The morphological changes of the cultured cells were observed and the growth curve drawn. The intracytoplasmic lipid of the cultured cells was determined using oil red O staining. RWSULRS: The cultured fibroblast-like cells showed highly homogeneous appearance with active proliferation, and could differentiate into mature adipocytes. Oil red O staining and morphological and biological observation verified these cells as preadipocytes with accumulation of lipid droplets in the cytoplasm. Extraction of the intracytoplasmic lipid stained with oil red O suggested a rapid increase in the intracytoplasmic lipid content on the 9th day of inoculation, which peaked on day 18. CONCLUSIONS: Preadipocytes are present in mature human adipose tissue and possess the potential to proliferate and differentiate into mature adipocytes. These homogeneous preadipocytes with good proliferating activity can be harvested through primary cell culture.

[Avastin combined with cisplan inhibits malignant ascites production in nude mice bearing transplanted ovary carcinoma with high VEGF expression].

OBJECTIVE: To establish a nude mouse model of malignant ascites with human ovarian carcinoma cell line OVCAR3 which highly expresses VEGF and evaluate the therapeutic of Avastin combined with cisplan. METHODS: Forty-eight nude mice with malignant ascites resulting from intraperitoneal transplantation of human ovarian carcinoma cell line OVCAR3 were treated with intraperitoneal injection of Avastin, cisplan, their combination, and PBS, respectively, to observe the effect on ascites development, VEGF content in the ascites, peritoneal permeability, development of new vessels and number of tumor cells in the ascites. RESULTS: Avastin obviously inhibited ascites accumulation and peritoneal capillary permeability, reduced VEGF protein level and microvascular density in the tumor tissues and the number of red cells and tumor cells in the malignant ascites, and prolonged the survival of the mice. The combination of Avastin and cisplan further enhanced the therapeutic efficacy of Avastin. CONCLUSION: The bio-chemotherapeutic strategy with Avastin combined with cisplan can be a promising method for treatment of malignant ascites.