Seonamhaeicola sediminis sp. nov., isolated from marine sediment.

A novel Gram-stain-negative, orange, rod or curved rod, facultatively anaerobic, gliding bacterial strain, designated strain W255T, was isolated from Xiaoshi Island, Weihai, China. The strain W255T grows optimally at a 28 °C, pH 7.5, and 3.0% (w/v) NaCl environment. Its colonies are circular, orange, non-transparent, smooth, and approximately 0.2-0.8 mm in diameter, after being cultured for 72 h on marine agar 2216. Cells of the strain W255T are 0.3-0.8 µm wide and 1.0-4.0 µm long, catalase-positive and oxidase-positive. The major cellular fatty acids are iso-C15:0, iso-C15:1 G, and iso-C15:0 3-OH. The sole respiratory quinone is MK-6. The major polar lipids include phosphatidylethanolamine, one unidentified amino lipid, one amino glycolipid, and two unidentified lipids (L1 and L2). The phylogenetic analysis based on 16S rRNA gene sequences indicated that strain W255T has the highest similarities with the type strain of the type species of the genus Seonamhaeicola, S. aphaedonensis KCTC 32578T (97.2%), and moderate with 'S. acroporae' KCTC 62713T (96.5%), S. algicola Gy8T (95.4%) and S. marinus B011T (94.5%). The ANI and dDDH values between strain W255T and S. aphaedonensis KCTC 32578T are 86.6% and 31.3%, respectively. The genomic DNA G + C content is 33.5 mol%. On the basis of gene annotation, it was observed that strain W255T have the abilities of nitrate reduction and utilizing various carbon sources, suggesting that this strain might be an important participant in the nitrogen cycle and carbon cycle in the marine environment. Based on the phenotypic, chemotaxonomic and phylogenetic analysis, strain W255T has been considered as a novel species of the genus Seonamhaeicola, for which the name Seonamhaeicola sediminis sp. nov. is proposed. The type strain is W255T (= MCCC 1H00377T = KCTC 72085T).

Aeromicrobium piscarium sp. nov., isolated from the intestine of Collichthys lucidus.

A Gram-stain-positive, rod-shaped, whitesmoke-coloured and aerobic bacterium, designated strain Co35T, was isolated from the intestine of Collichthys lucidus collected from the Jiangmen Guangdong Chinese White Dolphin Provincial Nature Reserve. Strain Co35T was able to grow at 15-35 °C (optimal 28 °C), at pH 7.0-8.5 (optimal 8.0) and with 0-9 % (w/v) NaCl (optimal 0.5-1 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Co35T was a member of the genus Aeromicrobium within the family Nocardioidaceae. The genomic DNA G+C content of strain Co35T was 68.4 mol%. Chemotaxonomic analysis showed that the sole respiratory quinone was menaquinone 9 (MK-9), and the major fatty acids included 10-methyl C18 : 0. The polar lipids were found to consist of phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylinositol (PI), two unidentified phospholipids (PL1-2) and two unidentified glycolipids (GL1-2). On the basis of its phylogenetic, phenotypic, chemotaxonomic, genotypic and genomic characteristics presented in this study, strain Co35T represents a novel species in the genus Aeromicrobium, for which the name Aeromicrobium piscarium sp. nov. is proposed. The type strain is Co35T (=KCTC 49280T=MCCC 1K03754T).