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1.
Eur J Oral Sci ; 125(6): 419-425, 2017 12.
Artículo en Inglés | MEDLINE | ID: mdl-29130547

RESUMEN

Human dental pulp stem cells (DPSCs) are oral mesenchymal stem cells with potential to differentiate into various cell types. Recent studies of DPSCs have focused on microRNAs (miRNAs), a class of small noncoding RNAs that play crucial roles in regulating DPSC phenotypes. In the current study, the expression of miR-140-5p was significantly decreased during lipopolysaccharide (LPS)-mediated differentiation of DPSCs in vitro. Overexpression of miR-140-5p enhanced proliferation of DPSCs and inhibited DPSC differentiation, whereas suppression of miR-140-5p produced the opposite effect. Moreover, the expression of toll-like receptor 4 (TLR-4), a critical regulator of DPSCs, was negatively correlated with the levels of miR-140-5p. A luciferase reporter analysis confirmed that miR-140-5p could regulate TLR-4 by directly binding to the 3'-untranslated region (3'-UTR) of the TLR4 mRNA. Additionally, we suppressed TLR-4 expression by treating cells with a TLR-4 inhibitor, CLI-095, and demonstrated that the effect of the miR-140-5p inhibitor on DPSC proliferation and differentiation could be partially reversed by blocking TLR-4. Taken together, our data suggest that miR-140-5p is a novel miRNA that regulates DPSC proliferation and differentiation.


Asunto(s)
Diferenciación Celular/genética , Proliferación Celular/genética , Pulpa Dental/citología , Células Madre Mesenquimatosas/metabolismo , MicroARNs/genética , Odontoblastos/metabolismo , Transducción de Señal/genética , Receptor Toll-Like 4/genética , Apoptosis , Western Blotting , Ciclo Celular , Supervivencia Celular , Células Cultivadas , Humanos , Técnicas In Vitro , Lipopolisacáridos , Fenotipo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
2.
Arch Oral Biol ; 118: 104838, 2020 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-32711339

RESUMEN

OBJECTIVE: The aim of the present study was to investigate the biological roles and underlying mechanism of the long non-coding RNA maternally expressed gene 3 (MEG3) on osteogenic differentiation of human dental pulp stem cells (hDPSCs). METHODS: The expression levels of MEG3, microRNA-543 (miR-543), osterix, osteopontin, osteocalcin and runt-related transcription factor 2 (RUNX2) were measured by quantitative real-time PCR (qRT-PCR). Alkaline phosphatase (ALP) activity assay and alizarin red S staining (ARS) were used to measure the impacts exerted by MEG3, miR-543 on osteogenic differentiation. Cell proliferation was measured by MTT assay. In addition, the targeted relationships between miR-543, MEG3, and Smad ubiquitin regulatory factor 1 (SMURF1) were assessed through dual luciferase reporter assay. RESULTS: During osteogenic induction, the expression of MEG3 was gradually reduced, whereas the expression of miR-543, osterix, osteopontin, osteocalcin and RUNX2 were gradually increased. Functional analysis implied that MEG3 overexpression or miR-543 inhibition reduced the cell proliferation, ALP activity, ARS levels, and decreased the expression of osteoblast-related proteins. Moreover, MEG3 promoted SMURF1 expression by directly targeting miR-543 as a competing endogenous RNA. Furthermore, overexpression of miR-543 or silencing SMURF1 could reverse the inhibitory effects of MEG3 on the osteogenic differentiation of hDPSCs. CONCLUSIONS: In conclusion, our study revealed that overexpression of MEG3 inhibited hDPSCs osteogenic differentiation via miR-543/SMURF1/RUNX2 regulatory network, which may contribute to the functional regulation and clinical applications of hDPSCs.


Asunto(s)
Subunidad alfa 1 del Factor de Unión al Sitio Principal/genética , MicroARNs/genética , Osteogénesis , ARN Largo no Codificante/genética , Células Madre/citología , Ubiquitina-Proteína Ligasas/genética , Diferenciación Celular , Células Cultivadas , Pulpa Dental/citología , Redes Reguladoras de Genes , Humanos , Osteocalcina/genética , Osteopontina/genética , Factor de Transcripción Sp7/genética
3.
Medicine (Baltimore) ; 99(22): e20372, 2020 May 29.
Artículo en Inglés | MEDLINE | ID: mdl-32481420

RESUMEN

The present study investigated the effectiveness of a Carisolv III + 0.5% sodium hypochlorite (NaOCl)-based root canal irrigant for smear layer removal.Forty maxillary incisors were randomly divided into 4 groups (n = 10 per group). The canals in group A (experimental) were prepared with 0.5% NaOCl, and Carisolv III and 0.5% NaOCl was used for the final washing; groups B and C (positive controls) used 2% and 5.25% NaOCl, respectively; and group D (negative control) used phosphate-buffered saline (PBS). Ethylenediaminetetraacetic acid (EDTA) was used for all of the groups. A 5-point scoring scale and scanning electron microscopy were used to evaluate the effectiveness of the irrigants. The canals were consistently cleaner in the coronal and middle thirds than in the apical thirds (P < .05).For cleaning the root canals, 5.25% NaOCl was more effective than 2% NaOCl, 0.5% NaOCl + Carisolv III, and phosphate-buffered saline , respectively (P < .05). The 2% NaOCl solution showed similar results to 0.5% NaOCl + Carisolv III (P > .05). The combination of 5.25% NaOCl and 17% EDTA remains the most effective irrigant for removal of the root canal smear layer.A combination of Carisolv III + 0.5% NaOCl (with 17% EDTA) showed a cleaning ability similar to that of 2% NaOCl (with 17% EDTA).


Asunto(s)
Ácido Glutámico/uso terapéutico , Leucina/uso terapéutico , Lisina/uso terapéutico , Irrigantes del Conducto Radicular/uso terapéutico , Tratamiento del Conducto Radicular/métodos , Hipoclorito de Sodio/uso terapéutico , Adulto , Cavidad Pulpar/cirugía , Femenino , Humanos , Técnicas In Vitro , Incisivo/cirugía , Masculino , Persona de Mediana Edad
4.
Medicine (Baltimore) ; 99(20): e20116, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-32443322

RESUMEN

The aim of this study was to explore the root morphology and root canal configuration of first premolars among Shandong Chinese residents using cone-beam computed tomography (CBCT).Randomly selected CBCT images were collected from 648 patients (44% women, 56% men). In total, 1268 maxillary and 1296 mandibular first premolars were analyzed. The number of roots and the canal configuration were recorded and identified based on Vertucci's classification.The majority of the maxillary first premolars had 1 root (67.4%), followed by 2 roots (32%). A 2-canal configuration (89%) was the most prevalent observation. For mandibular first premolars, 98.8% had 1 root and 81% presented the type I configuration. There were no statistical differences in the number of roots or morphology in terms of the left/right side or sex (P > .05).Among Chinese residents, the majority of maxillary first premolars had 1 root and 2 canals, whereas the most common anatomical configuration for mandibular first premolars was 1 root with 1 canal.


Asunto(s)
Diente Premolar/diagnóstico por imagen , Tomografía Computarizada de Haz Cónico , Raíz del Diente/diagnóstico por imagen , Adolescente , Adulto , Anciano , Variación Anatómica , Pueblo Asiatico , Femenino , Humanos , Masculino , Mandíbula , Maxilar , Persona de Mediana Edad , Adulto Joven
5.
Pathol Oncol Res ; 26(3): 1677-1685, 2020 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-31598896

RESUMEN

Similar to the mesenchymal stem cells (MSCs), dental pulp stem cells (DPSCs) also have pluripotent differentiation characteristic and may be more ideal for tissue regeneration, especially in tooth regeneration engineering. However, bacterial infection may be a powerful obstacle. Berberine (BBR), known with antibacterial effects, was recently found to play functions in bone formation through promoting osteogenic differentiation from pluripotent stem cells. However, whether BBR also function in DPSCs osteogenic differentiation has not yet been reported. Primary DPSCs were isolated from dental pulp tissues extracted from human impacted mandibular third molars, and identified by flow cytometry for cell surface antigen molecules. A dexamethasone osteogenic medium was used to induce DPSCs osteogenic differentiation. BBR (1 µM and 5 µM) was pre-added to into medium, and then cell proliferation, spheroid formation and osteogenic differentiation capacities of DPSCs were analyzed, as well as the underlying molecules modulation mechanism. Flow cytometry identified that CD44, CD90, CD81 and CD105 positively expressed in the isolated hDPSCs, with CD34 and CD45 negetively expressed. BBR enhanced the cell proliferation of hDPSCs in a dose-dependent pattern, and promoted dexamethasone-induced osteogenic differentiation via enhancing Runx2 transcription factor activity followed by upregulating osteogenesis markers expression, whereas the adipogenic differentiation of hDPSCs was suppressed dramatically by BBR. The EGFR and MAPK pathways were activated by BBR, and inhibitors for these pathways significantly suppressed the osteogenic differentiation promotion of BBR. These results have revealed a novel mechanism that berberine might promote hDPSCs osteogenic differentiation through activating EGFR-MAPK-Runx2 signaling pathways.


Asunto(s)
Berberina/farmacología , Diferenciación Celular/efectos de los fármacos , Pulpa Dental/citología , Osteogénesis/efectos de los fármacos , Células Madre Pluripotentes/efectos de los fármacos , Transducción de Señal/efectos de los fármacos , Adolescente , Adulto , Proliferación Celular/efectos de los fármacos , Células Cultivadas , Subunidad alfa 1 del Factor de Unión al Sitio Principal/efectos de los fármacos , Subunidad alfa 1 del Factor de Unión al Sitio Principal/metabolismo , Receptores ErbB/efectos de los fármacos , Receptores ErbB/metabolismo , Humanos , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Sistema de Señalización de MAP Quinasas/fisiología , Adulto Joven
6.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 36(5): 576-579, 2018 Oct 01.
Artículo en Zh | MEDLINE | ID: mdl-30465356

RESUMEN

The root and canal anatomy of the mandibular first molar is highly complicated. A mandibular first molar with a C-shaped root canal system is rare. This article describes two different cases of this system. Case 1 has a complete C-shaped fused root. The root canal is characterized by a mesial lingual root canal and a C-shaped fused root canal. Case 2 has a C-shaped fused root in the buccal region and a distal lingual root. The root canal is characterized by a type Ⅱ-Ⅰroot canal in the buccal region and a distal lingual root canal.


Asunto(s)
Cavidad Pulpar , Mandíbula , Tratamiento del Conducto Radicular , Humanos , Diente Molar , Raíz del Diente
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