Uneven Strain Distribution Induces Consecutive Dislocation Slipping, Plane Gliding, and Subsequent Detwinning of Penta-Twinned Nanoparticles.

Twin structures possess distinct physical and chemical properties by virtue of their specific twin configuration. However, twinning and detwinning processes are not fully understood on the atomic scale. Integrating in situ high resolution transmission electron microscopy and molecular dynamic simulations, we find tensile strain in the asymmetrical 5-fold twins of Au nanoparticles leads to twin boundary migration through dislocation sliding (slipping of an atomic layer) along twin boundaries and dislocation reactions at the 5-fold axis under an electron beam. Migration of one or two layers of twin planes is governed by energy barriers, but overall, the total energy, including surface, lattice strain, and twin boundary energy, is relaxed after consecutive twin boundary migration, leading to a detwinning process. In addition, surface rearrangement of 5-fold twinned nanoparticles can aid in the detwinning process.

Synchronously Improved Multiple Afterglow and Phosphorescence Efficiencies in 0D Hybrid Zinc Halides with Ultrahigh Anti-Water Stabilities.

Zero-dimensional (0D) hybrid metal halides have been emerged as room-temperature phosphorescence (RTP) materials, but synchronous optimization of multiple phosphorescence performance in one structural platform remains less resolved, and stable RTP activity in aqueous medium is also unrealized due to serious instability toward water and oxygen. Herein, we demonstrated a photophysical tuning strategy in a new 0D hybrid zinc halide family of (BTPP)2ZnX4 (BTPP = benzyltriphenylphosphonium, X = Cl and Br). Infrequently, the delicate combination of organic and inorganic species enables this family to display multiple ultralong green afterglow and efficient self-trapped exciton (STE) associated cyan phosphorescence. Compared with inert luminescence of [BTPP]+ cation, incorporation of anionic [ZnX4]2- effectively enhance the spin-orbit coupling effect, which significantly boosts the photoluminescence quantum yield (PLQY) up to 30.66% and 54.62% for afterglow and phosphorescence, respectively. Synchronously, the corresponding luminescence lifetime extend to 143.94 ms and 0.308 µs surpassing the indiscernible phosphorescence of [BTPP]X salt. More importantly, this halide family presents robust RTP emission with nearly unattenuated PLQY in water and harsh condition (acid and basic aqueous solution) over half a year. The highly efficient integrated afterglow and STE phosphorescence as well as ultrahigh aqueous state RTP realize multiple anti-counterfeiting applications in wide chemical environments.

Influence of Gravity on the Sliding Angle of Water Drops on Nanopillared Superhydrophobic Surfaces.

Molecular dynamics (MD) simulations were used to study the effects of gravity, solid surface energy, and the fraction of water-solid interface area on the water droplet sliding angles on nanopillared surfaces. To effectively simulate the influence of gravity on drop sliding, we developed a protocol in which we scale the value of gravitational acceleration used in our simulations according to the Bond number (Bo). In this way, we approximate the behavior of drops larger than we can effectively simulate using MD. The sliding angle decreased with an increase in Bo, while it increased with an increase in the liquid-solid surface interaction. The sliding angles exhibit a minimum with an increase in the fraction of water-solid interface area, due to meniscus formation at high fractions. Trends predicted by our model are in agreement with experiment. Using our model, we investigated the mechanisms of droplet movement along nanopillared surfaces. Depending on the pinning state of the droplets at equilibrium, either the advancing or the receding contact angle initiates motion. Moreover, the minimum dynamic advancing and receding contact angles of drops with gravity are close to the static contact angle and the intrinsic contact angle, respectively, while the maxima of both angles are as large as 180°. We find that the drops move through a combination of sliding and rolling, in agreement with experiment. Our studies offer clarity to conflicting experimental reports and present new results awaiting experimental confirmation.

Dynamic Contact Angles and Mechanisms of Motion of Water Droplets Moving on Nanopillared Superhydrophobic Surfaces: A Molecular Dynamics Simulation Study.

In this work, we investigate the dynamic advancing and receding contact angles, and the mechanisms of motion of water droplets moving across nanopillared superhydrophobic surfaces using molecular-dynamics simulation. We obtain equilibrium Cassie states of droplets on nanopillared surfaces with different pillar heights, groove widths, and intrinsic contact angles. We quantitatively evaluate the dynamic advancing and receding contact angles along the advancing direction of an applied body force, and find that they depend on the roughness parameters and the applied body force in a predictable way. The maximum dynamic advancing contact angle is 180°, and the minimum dynamic advancing contact angle is close to the static contact angle. On the receding side, the maximum dynamic receding contact angle is as large as 180°, while the minimum dynamic receding contact angle is close to the intrinsic contact angle of smooth surface. Interestingly, water droplets exhibit a "rolling" mechanism as they move across the surface, which is confirmed by movies of interfacial water molecules, as well as droplet velocity profiles.

Size and temperature dependent shapes of copper nanocrystals using parallel tempering molecular dynamics.

We performed parallel-tempering molecular dynamics simulations to predict the temperature- and size-dependent equilibrium shapes of a series of Cu nanocrystals in the 100- to 200-atom size range. Our study indicates that temperature-dependent, solid-solid shape transitions occur frequently for Cu nanocrystals in this size range. Complementary calculations with electronic density functional theory indicate that vibrational entropy favors nanocrystals with a shape intermediate between a decahedron and an icosahedron. Overall, we find that entropy plays a significant role in determining the shapes Cu nanocrystals, so studies aimed at determining minimum-energy shapes may fail to correctly predict shapes observed at experimental temperatures. We also observe significant shape changes with nanocrystal size - sometimes with changes in a single atom. The information from this study could be useful in efforts to devise processing routes to achieve selective nanocrystal shapes.

Hybrid metal halide family with color-time-dual-resolved phosphorescence for multiplexed information security applications.

Luminescent materials with engineered optical properties play an important role in anti-counterfeiting and information security technology. However, conventional luminescent coding is limited by fluorescence color or intensity, and high-level multi-dimensional luminescent encryption technology remains a critically challenging goal in different scenarios. To improve the encoding capacity, we present an optical multiplexing concept by synchronously manipulating the emission color and decay lifetimes of room-temperature phosphorescence materials at molecular level. Herein, we devise a family of zero-dimensional (0D) hybrid metal halides by combining organic phosphonium cations and metal halide tetrahedral anions as independent luminescent centers, which display blue phosphorescence and green persistent afterglow with the highest quantum yields of 39.9 % and 57.3 %, respectively. Significantly, the luminescence lifetime can be fine-tuned in the range of 0.0968-0.5046 µs and 33.46-125.61 ms as temporary time coding through precisely controlling the heavy atomic effect and inter-molecular interactions. As a consequence, synchronous blue phosphorescence and green afterglow are integrated into one 0D halide platform with adjustable emission lifetime acting as color- and time-resolved dual RTP materials, which realize the multiple applications in high-level anti-counterfeiting and information storage. The color-lifetime-dual-resolved encoding ability greatly broadens the scope of luminescent halide materials for optical multiplexing applications.

Minimum Free-Energy Shapes of Ag Nanocrystals: Vacuum vs Solution.

We use two variants of replica-exchange molecular dynamics (MD) simulations, parallel tempering MD and partial replica exchange MD, to probe the minimum free-energy shapes of Ag nanocrystals containing 100-200 atoms in a vacuum, ethylene glycol (EG) solvent, and EG solvent with a PVP polymer containing 100 repeat units. Our simulations reveal a shape intermediate between a Dh and an Ih, a Dh-Ih, that has distinct structural signatures and magic sizes. We find several prominent features associated with entropy: pure FCC nanocrystals are less common than FCC crystals containing stacking faults, and crystals with the minimum potential energy are not always preferred over the range of relevant temperatures. The shapes of the nanocrystals in solution are influenced by the chemical identities of the solution-phase molecules. Comparing Ag nanocrystal shapes in EG to those in an EG+PVP solution, we find more icosahedra in EG and more decahedra in EG+PVP across all of the nanocrystal sizes probed in this study. At certain critical sizes, nanocrystal shapes can change dramatically with the addition and removal of a single atom or with a change in temperature at a fixed size. The information in our study could be useful in efforts to devise processing routes to achieve selective nanocrystal shapes.

Application of Zero-Watermarking Scheme Based on Swin Transformer for Securing the Metaverse Healthcare Data.

The existing medical image privacy solutions cannot completely solve the security problems created by applying the metaverse healthcare system. A robust zero-watermarking scheme based on the Swin Transformer is proposed in this paper to improve the security of medical images in the metaverse healthcare system. This scheme uses a pretrained Swin Transformer to extract deep features from the original medical images with a good generalization performance and multiscale, and binary feature vectors are generated by using the mean hashing algorithm. Then, the logistic chaotic encryption algorithm boosts the security of the watermarking image by encrypting it. Finally, an encrypted watermarking image is XORed with the binary feature vector to create a zero-watermarking, and the validity of the proposed scheme is verified through experimentation. According to the results of the experiments, the proposed scheme has excellent robustness to common attacks and geometric attacks, and implements privacy protections for medical image security transmissions in the metaverse. The research results provide a reference for the data security and privacy protection of the metaverse healthcare system.

Harnessing the power of optical microscopy for visualization and analysis of histopathological images.

Histopathology is the foundation and gold standard for identifying diseases, and precise quantification of histopathological images can provide the pathologist with objective clues to make a more convincing diagnosis. Optical microscopy (OM), an important branch of optical imaging technology that provides high-resolution images of tissue cytology and structural morphology, has been used in the diagnosis of histopathology and evolved into a new disciplinary direction of optical microscopic histopathology (OMH). There are a number of ex-vivo studies providing applicability of different OMH approaches, and a transfer of these techniques toward in vivo diagnosis is currently in progress. Furthermore, combined with advanced artificial intelligence algorithms, OMH allows for improved diagnostic reliability and convenience due to the complementarity of retrieval information. In this review, we cover recent advances in OMH, including the exploration of new techniques in OMH as well as their applications, and look ahead to new challenges in OMH. These typical application examples well demonstrate the application potential and clinical value of OMH techniques in histopathological diagnosis.

Label-free structural and functional volumetric imaging by dual-modality optical-Raman projection tomography.

Mesoscale volumetric imaging is of great importance for the study of bio-organisms. Among others, optical projection tomography provides unprecedented structural details of specimens, but it requires fluorescence label for chemical targeting. Raman spectroscopic imaging is able to identify chemical components in a label-free manner but lacks microstructure. Here, we present a dual-modality optical-Raman projection tomography (ORPT) technology, which enables label-free three-dimensional imaging of microstructures and components of millimeter-sized samples with a micron-level spatial resolution on the same device. We validate the feasibility of our ORPT system using images of polystyrene beads in a volume, followed by detecting biomolecules of zebrafish and Arabidopsis, demonstrating that fused three-dimensional images of the microstructure and molecular components of bio-samples could be achieved. Last, we observe the fat body of Drosophila melanogaster at different developmental stages. Our proposed technology enables bimodal label-free volumetric imaging of the structure and function of biomolecules in a large sample.

Sensitivity improved Cerenkov luminescence endoscopy using optimal system parameters.

BACKGROUND: The challenges of clinical translation of optical imaging, including the limited availability of clinically used imaging probes and the restricted penetration depth of light propagation in tissues can be avoided using Cerenkov luminescence endoscopy (CLE). However, the clinical applications of CLE are limited due to the low signal level of Cerenkov luminescence and the large transmission loss caused by the endoscope, which results in a relatively low detection sensitivity of current CLE. The aim of this study was to enhance the detection sensitivity of the CLE system and thus improve the system for clinical application in the detection of gastrointestinal diseases. METHODS: Four optical fiber endoscopes were customized with different system parameters, including monofilament (MF) diameter of imaging fiber bundles, fiber material, probe coating, etc. The endoscopes were connected to the detector via a specifically designed straight connection device to form the CLE system. The ß-2-[18F]-Fluoro-2-deoxy-D-glucose (18F-FDG) solution and the radionuclide of Gallium-68 (68Ga) were used to evaluate the performance of the CLE system. The images of the 18F-FDG solution acquired by the CLE were used to optimize imaging parameters of the system. By using the endoscope with optimized parameters, including the MF diameter of imaging fiber bundles, fiber materials, etc., the resolution and sensitivity of the assembled CLE system were measured by imaging the radionuclide of 68Ga. RESULTS: The results of 18F-FDG experiments showed that larger MF diameter led to higher collection efficiency. The fiber material and probe coating with high transmission ratios in the range of 400-900 nm also increased signal collection and transmission efficiency. The results of 68Ga evaluations showed that a minimum radioactive activity of radionuclides as low as 0.03 µCi was detected in vitro within 5 minutes, while that of 0.68 µCi can be detected within 1 minute. In vivo experiments also demonstrated that the developed CLE system achieved a high sensitivity at a submicrocurie level; that is, 0.44 µCi within 5 minutes, and 0.83 µCi within 1 minute. The weaker in vivo sensitivity was due to the attenuation of the signal by the mouse tissue skin and the autofluorescence interference produced by biological tissues. CONCLUSIONS: By optimizing the structural parameters of fiber endoscope and imaging parameters for data acquisition, we developed a CLE system with a sensitivity at submicrocurie level. These results support the possibility that this technology can clinically detect early tumors within 1 minute.

Confocal Laser Scanning Microscopy Based on a Silicon Photomultiplier for Multicolor In Vivo Imaging in Near-Infrared Regions I and II.

Confocal laser scanning microscopy (CLSM) is expected to exhibit a better imaging performance in the second near-infrared (NIR-II) windows with weak tissue scattering and autofluorescence. However, the indium gallium arsenide (InGaAs) detectors currently used for imaging in the NIR-II region are prohibitively expensive, hampering its extensive biomedical applications. In this study, a novel NIR-II CLSM system is developed by using the inexpensive silicon photomultiplier (SiPM) that can perform the multicolor biological imaging in vivo. Using IR-780 iodide as the contrast agent, the NIR-II imaging capability of constructed CLSM is inspected, demonstrating a spatial resolution of 1.68 µm (close to the diffraction limit) and a fluorophore detection sensitivity as low as 100 nm. In particular, it is discovered that the multicolor imaging performance in both NIR-I and NIR-II windows is comparable to those from multialkali and InGaAs photomultiplier tubes. In addition, 3D NIR-II CLSM is also conducted for in vivo imaging of the vascular structure in mouse ear and subcutaneous tumors. To the best of authors' knowledge, this is the first time that a low-cost detector based on a SiPM has been used for microscopic imaging of trailing fluorescence signals in the NIR-II region of an NIR fluorescent probe.

Self-Assembly of a Linear Alkylamine Bilayer around a Cu Nanocrystal: Molecular Dynamics.

Copper nanocrystals are often grown with the help of alkylamine capping agents, which direct the nanocrystal shape. However, the role of these molecules is still unclear. We characterized the assembly of aqueous tetradecylamine (TDA) around a Cu nanocrystal and found that TDA exhibits a temperature-dependent bilayer structure. The bilayer involves an inner layer, in which TDA binds to Cu via the amine group and tends to orient the alkyl tail perpendicular to the surface, and an outer layer whose structure depends on temperature. At low temperatures, alkylamines in the inner layer form bundles with no apparent relation to the crystal facets. Alkylamines in the outer layer tend to orient their long axes perpendicular to the Cu surfaces, with interdigitation into the inner layer. At high temperatures, alkylamines in the inner layer lose their bundle structure, and outer-layer alkylamines tend to orient themselves tangential to the Cu surfaces, forming a "web" above inner-layer TDA. TDA exhibits a rapid interlayer exchange at typical synthesis temperatures, consistent with experiment. The variety in the assemblies seen here and in other studies of alkanethiols around gold nanocrystals indicates a richness in the assemblies that can be achieved by modulating the interaction between the strongly binding end group and the surface.

Oriented attachment mechanism of triangular Ag nanoplates: a molecular dynamics study.

We use molecular-dynamics simulations to probe the experimentally observed aggregation of PVP-covered triangular Ag nanoplates to form 2D sheets in solution. We find lateral plate attachment is the most favorable aggregation pathway - consistent with experiment. The mechanism is general and suggests new processing strategies for creating 2D architectures in solution-phase syntheses.

Growth Mechanism of Five-Fold Twinned Ag Nanowires from Multiscale Theory and Simulations.

Five-fold twinned metal nanowires can be synthesized with high aspect ratios via solution-phase methods. The origins of their anisotropic growth, however, are poorly understood. We combine atomic-scale, mesoscale, and continuum theoretical methods to predict growth morphologies of Ag nanowires from seeds and to demonstrate that high aspect ratio nanowires can originate from anisotropic surface diffusion induced by the strained nanowire structure. Nanowire seeds are similar to Marks decahedra, with {111} "notches" that accelerate diffusion along the nanowire axis to facilitate one-dimensional growth. The strain distribution on the {111} facets induces heterogeneous atom aggregation and leads to atom trapping at the nanowire ends. We predict that decahedral Ag seeds can grow to become nanowires with aspect ratios in the experimental range. Our studies show that there is a complex interplay between atom deposition, diffusion, seed architecture, and nanowire aspect ratio that could be manipulated experimentally to achieve controlled nanowire syntheses.

Harnessing the Power of Optical Microscopic and Macroscopic Imaging for Natural Products as Cancer Therapeutics.

Natural products (NPs) are an important source for new drug discovery over the past decades, which have been demonstrated to be effectively used in cancer prevention, treatment, and adjuvant therapy. Many methods, such as the genomic and metabolomic approaches, immunochemistry, mass spectrometry, and chromatography, have been used to study the effects of NPs on cancer as well as themselves. Because of the advantages in specificity, sensitivity, high throughput, and cost-effectiveness, optical imaging (OI) approaches, including optical microscopic imaging and macroscopic imaging techniques have also been applied in the studies of NPs. Optical microscopic imaging can observe NPs as cancer therapeutics at the cellular level and analyze its cytotoxicity and mechanism of action. Optical macroscopic imaging observes the distribution, metabolic pathway, and target lesions of NPs in vivo, and evaluates NPs as cancer therapeutics at the whole-body level in small living animals. This review focuses on the recent advances in NPs as cancer therapeutics, with particular emphasis on the powerful use of optical microscopic and macroscopic imaging techniques, including the studies of observation of ingestion by cells, anticancer mechanism, and in vivo delivery. Finally, we prospect the wider application and future potential of OI approaches in NPs as cancer therapeutics.