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1.
J Bacteriol ; 206(6): e0027323, 2024 Jun 20.
Artículo en Inglés | MEDLINE | ID: mdl-38717111

RESUMEN

Type VI secretion system (T6SS) is a potent weapon employed by various Pseudomonas species to compete with neighboring microorganisms for limited nutrients and ecological niches. However, the involvement of T6SS effectors in interbacterial competition within the phytopathogen Pseudomonas syringae remains unknown. In this study, we examined two T6SS clusters in a wild-type P. syringae MB03 and verified the involvement of one cluster, namely, T6SS-1, in interbacterial competition. Additionally, our results showed that two T6SS DNase effectors, specifically Tde1 and Tde4, effectively outcompeted antagonistic bacteria, with Tde4 playing a prominent role. Furthermore, we found several cognate immunity proteins, including Tde1ia, Tde1ib, and Tde4i, which are located in the downstream loci of their corresponding effector protein genes and worked synergistically to protect MB03 cells from self-intoxication. Moreover, expression of either Tde1 or C-terminus of Tde4 in Escherichia coli cells induced DNA degradation and changes in cell morphology. Thus, our results provide new insights into the role of the T6SS effectors of P. syringae in the interbacterial competition in the natural environment. IMPORTANCE: The phytopathogen Pseudomonas syringae employs an active type VI secretion system (T6SS) to outcompete other microorganisms in the natural environment, particularly during the epiphytic growth in the phyllosphere. By examining two T6SS clusters in P. syringae MB03, T6SS-1 is found to be effective in killing Escherichia coli cells. We highlight the excellent antibacterial effect of two T6SS DNase effectors, namely, Tde1 and Tde4. Both of them function as nuclease effectors, leading to DNA degradation and cell filamentation in prey cells, ultimately resulting in cell death. Our findings deepen our understanding of the T6SS effector repertoires used in P. syringae and will facilitate the development of effective antibacterial strategies.


Asunto(s)
Proteínas Bacterianas , Desoxirribonucleasas , Pseudomonas syringae , Sistemas de Secreción Tipo VI , Pseudomonas syringae/genética , Pseudomonas syringae/metabolismo , Pseudomonas syringae/enzimología , Sistemas de Secreción Tipo VI/metabolismo , Sistemas de Secreción Tipo VI/genética , Proteínas Bacterianas/metabolismo , Proteínas Bacterianas/genética , Desoxirribonucleasas/metabolismo , Desoxirribonucleasas/genética , Regulación Bacteriana de la Expresión Génica , Escherichia coli/genética , Escherichia coli/metabolismo , Escherichia coli/efectos de los fármacos
2.
Small ; 20(2): e2304404, 2024 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-37670529

RESUMEN

Cyano-rich g-C3 N4 materials are widely used in various fields of photochemistry due to the very powerful electron-absorbing ability and electron storage function of cyano, as well as its advantages in improving light absorption, adjusting the energy band structure, increasing the polarization rate and electron density in the structure, active site concentration, and promoting oxygen activation ability. Notwithstanding, there is yet a huge knowledge break in the design, preparation, detection, application, and prospect of cyano-rich g-C3 N4 . Accordingly, an overall review is arranged to substantially comprehend the research progress and position of cyano-rich g-C3 N4 materials. An overall overview of the current research position in the synthesis, characterization (determination of their location and quantity), application, and reaction mechanism analysis of cyano-rich g-C3 N4 materials to provide a quantity of novel suggestions for cyano-modified carbon nitride materials' construction is provided. In view of the prevailing challenges and outlooks of cyano-rich g-C3 N4 materials, this paper will purify the growth direction of cyano-rich g-C3 N4 , to achieve a more in-depth exploration and broaden the applications of cyano-rich g-C3 N4 .

3.
Environ Res ; 223: 115445, 2023 04 15.
Artículo en Inglés | MEDLINE | ID: mdl-36758915

RESUMEN

The development of cost-effective passivators for the remediation of heavy metal-contaminated soils has been a research hotspot and an unsolved challenge. Herein, a novel hydroxyapatite (GSCH) was synthesized by co-precipitating distiller effluent-derived Ca with (NH4)2HPO4 using straw-derived dissolved organic matter (S-DOM) as the dispersant. Batch adsorption experiments and soil incubation tests were performed to assess the immobilization efficiency of GSCH for Cd in weakly alkaline environments. As a result, GSCH showed an excellent adsorption efficiency to Cd with a maximum adsorption amount of ∼222 mg g-1, which was fairly competitive compared to other similar previously materials reported. The kinetic data indicated that the adsorption of Cd on GSCH was a chemical and irreversible process, while the thermodynamic data revealed a spontaneous (ΔG° < 0) and endothermic (ΔH° > 0) adsorption process. Based on mechanism analysis, both physisorption (e.g., electrostatic attraction and pore filling) and chemisorption (e.g., ion exchange and complexation) were responsible for Cd adsorption on GSCH. Particularly, the incorporated S-DOM and hydroxyapatite phase in GSCH acted synergistically in the adsorption process. The incubation results showed that GSCH application could significantly reduce the bioavailability, phytoavailability and bioaccessibility of Cd in soil by 48.4%-57.8%, 20.4%-28.6% and 12.6%-24.0%, respectively. Moreover, GSCH application also improved soil bacterial communities and enhanced soil nutrient availability. Overall, this is the first study to demonstrate the potential application value of GSCH in Cd immobilization, providing promising insights into the development of green and cost-effective hydroxyapatite-based passivators for the remediation of heavy metal-contaminated soils.


Asunto(s)
Metales Pesados , Contaminantes del Suelo , Cadmio/análisis , Durapatita , Contaminantes del Suelo/análisis , Suelo , Metales Pesados/análisis , Adsorción
4.
Molecules ; 28(15)2023 Jul 30.
Artículo en Inglés | MEDLINE | ID: mdl-37570727

RESUMEN

As a kind of biomass resource, waste office paper can be used as a carbon precursor to prepare carbon materials. In this work, carbon microspheres with regular shape, uniform particle size and high carbon content were successfully prepared from waste office paper via a hydrothermal synthesis method with sulfuric acid as the catalyst. The effects of reaction temperature and sulfuric acid dosage on the morphology of the carbon microspheres were studied. The formation mechanism of the carbon microspheres was investigated by analyzing the structure and composition of the products. The results show that the hydrolysis of cellulose in waste paper under hydrothermal conditions was the key for the formation of carbon microspheres. The temperature of hydrothermal reaction and the use of sulfuric acid can affect the morphology of carbon microspheres. The carbon microspheres synthesized at 210 °C with 10 mL sulfuric acid have the best surface morphology, with uniform particle size and higher dispersion. Cyclic voltammetry and electrochemical impedance spectroscopy show that the carbon microspheres have good capacitance performance and can be used in capacitors. This study provides a low-cost precursor for carbon microspheres as well as a new method for the recycle of waste paper.

5.
Cancer Invest ; 40(2): 91-102, 2022 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-34515610

RESUMEN

Surgical removal of the tumor is currently the first-line treatment for lung cancer, but the procedure may accelerate cancer progression through immunosuppression. However, whether CCL2 (C-C motif chemokine ligand 2) enhances cancer progression by affecting regulatory T cells (Tregs) remains unknown. We found that the volume and weight of tumors were larger in the surgical trauma group than in the control group. CCL2 expression and Treg abundance were increased in tumor tissues after surgical trauma, and CCL2 expression was positively associated with Treg abundance. These results demonstrated that surgical trauma contributes to lung cancer progression by increasing CCL2 expression, thus promoting Treg recruitment.


Asunto(s)
Carcinoma de Pulmón de Células no Pequeñas/patología , Quimiocina CCL2/metabolismo , Neoplasias Pulmonares/patología , Toracotomía/efectos adversos , Regulación hacia Arriba , Células A549 , Anciano , Animales , Carcinoma de Pulmón de Células no Pequeñas/metabolismo , Estudios de Casos y Controles , Progresión de la Enfermedad , Femenino , Regulación Neoplásica de la Expresión Génica , Humanos , Neoplasias Pulmonares/metabolismo , Masculino , Ratones , Persona de Mediana Edad , Trasplante de Neoplasias , Linfocitos T Reguladores/metabolismo
6.
Environ Sci Technol ; 54(20): 12989-12997, 2020 10 20.
Artículo en Inglés | MEDLINE | ID: mdl-32915555

RESUMEN

Ubiquitous organo-ferrihydrite coprecipitates (OFC) significantly affect the mobility and availability of Cr in soil through sorption, but the underlying sorption mechanisms remain unclear at the molecular level. Due to the potential formation of OFC in agricultural soils with returned crop straws, we synthesized OFC with rice/rape straw-derived carbon (C) sources and different loadings. The molecular sorption mechanisms of Cr(III) to the synthesized OFC under different conditions were investigated by Cr K-edge extended X-ray absorption fine structure (EXAFS) spectroscopy and scanning transmission X-ray microscopy (STXM). Cr(III) sorption by OFC decreased with increasing C loading and decreasing pH, regardless of C sources. Moreover, inhibition of Cr(III) sorption to OFC with high C loading occurred when ionic strength (IS) increased, suggesting the presence of outer-sphere complexed Cr(III). EXAFS analysis revealed that more Cr(III) were bound to ferrihydrite of the OFC at a relatively high pH, and organically bound Cr(III) enhanced when increasing C loading and decreasing IS. STXM analysis strongly suggested that C loading reduced Cr(III) sorption through blocking the binding sites on the ferrihydrite, which overwhelmed Cr(III) retention by the direct binding of Cr(III) to carboxyl of the particulate organic matter (OM) and OM coated on the Fh fractions of the OFC. These findings facilitated the comprehensive understanding of the sorption mechanisms of Cr(III) to OFC at the molecular level, which will assist the prediction of Cr(III) mobility in soils, particularly for Cr(III)-contaminated agricultural soils with the application of crop straws.


Asunto(s)
Compuestos Férricos , Sincrotrones , Adsorción , Espectroscopía de Absorción de Rayos X , Rayos X
7.
Water Sci Technol ; 81(10): 2270-2280, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-32701504

RESUMEN

It is of great significance to remove Cr(VI) from water as a result of its high toxicity. Biochar from corn straw was modified by different acids (HNO3, H2SO4 and H3PO4) to remove Cr(VI) from aqueous solution. To estimate the removal mechanisms of Cr(VI) by the acid-modified biochars, batch experiments were performed in the light of contact time, Cr(VI) concentration, and pH, and the characteristics of acid-modified biochars before and after Cr(VI) adsorption were investigated by Fourier transform infrared spectra (FTIR) and X-ray photoelectron spectroscopy (XPS). The adsorption kinetics of Cr(VI) by acid-modified biochars were consistent with the pseudo-second-order model, and the adsorption isotherm obeyed the Freundlich model. Furthermore, the acid- modified biochars could supply more oxygen-containing functional groups (-COOH and -OH) as electron donor (e-) and hydrogen ion (H+) to enhance the reduction of Cr(VI) to Cr(III), resulting in enhanced removal of Cr(VI). HNO3-modified biochar exhibited the highest removal efficiency of Cr(VI). In general, the acid modifition of biochar was an effective method to increase the removal of Cr(VI).


Asunto(s)
Contaminantes Químicos del Agua/análisis , Zea mays , Adsorción , Carbón Orgánico , Cromo/análisis , Concentración de Iones de Hidrógeno , Cinética
8.
J Environ Sci Health B ; 55(11): 941-950, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32715911

RESUMEN

Animal manures usually contain high contents of heavy metals (HMs) and thus pose a considerable threat to human health and environment when applied to soil. In this study, the effect of pyrolysis temperature (300 °C, 400 °C, 500 °C, 600 °C, and 700 °C) on the properties of biochar produced from chicken manure was studied. In addition, the response of speciation, bioavailability, leachability, and environmental risk of HMs in biochar to different pyrolysis temperature was investigated. The results showed that biochars pyrolyzed at high temperatures generally had high pH, ash content, surface area, and stability. As the pyrolysis temperature increased, the total concentrations of Cu, Zn, Cr, and Ni continually increased, whereas those of Pb and Cd initially increased and then decreased. Moreover, the transformation of the bioavailable fractions of HMs into stable fractions obviously increased with increasing pyrolysis temperature. Thus, pyrolysis at high temperature led to a further decrease in the bioavailability, leachability, and environmental risk of six HMs, as shown by DTPA, TCLP, and the potential ecological risk index. Overall, it is convincing that that 700 °C is the optimal temperature when considering the quality and environmental safety of biochar derived from chicken manure.


Asunto(s)
Carbón Orgánico/química , Estiércol , Metales Pesados/química , Administración de Residuos/métodos , Animales , Disponibilidad Biológica , Pollos , Calor , Metales Pesados/análisis , Metales Pesados/farmacocinética , Pirólisis , Factores de Riesgo , Contaminantes del Suelo/análisis , Contaminantes del Suelo/farmacocinética , Espectroscopía Infrarroja por Transformada de Fourier
10.
Water Sci Technol ; 73(5): 1112-21, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-26942534

RESUMEN

In this paper, spinel ferrite with high crystallinity and high saturation magnetization was successfully prepared from steel pickling sludge by adding iron source and precipitator in the hydrothermal condition. The obtained spinel ferrite was characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), vibrating sample magnetometer (VSM), and Zeta potential methods and investigated as an adsorbent for removal of Pb(2+) from aqueous solution. Batch experiments were performed by varying the pH values, contact time, temperature and initial metal concentration. The result of pH impact showed that the adsorption of Pb(2+) was a pH dependent process, and the pH 5.8 ± 0.2 was found to be the optimum condition. The achieved experimental data were analyzed with various kinetic and isotherm models. The kinetic studies revealed that Pb(2+) adsorption onto spinel ferrite followed a pseudo-second order model, and the Langmuir isotherm model provided the perfect fit to the equilibrium experimental data. At different temperatures, the maximum Pb(2+) adsorption capacities calculated from the Langmuir equation were in the range of 126.5-175.4 mg/g, which can be in competition with other adsorbents. The thermodynamic results showed that the spinel ferrite could spontaneously and endothermically adsorb Pb(2+) from aqueous solution. The regeneration studies showed that spinel ferrite could be used five times (removal efficiency (%) >90%) by desorption with HNO3 reagent.


Asunto(s)
Compuestos Férricos/química , Plomo/química , Eliminación de Residuos Líquidos/métodos , Contaminantes Químicos del Agua/química , Adsorción , Residuos Industriales/análisis , Cinética , Acero , Temperatura , Termodinámica , Difracción de Rayos X
11.
J Environ Sci (China) ; 26(5): 970-80, 2014 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-25079627

RESUMEN

Alkaline residue (AR) was found to be an efficient adsorbent for phosphate removal from wastewater. The kinetic and equilibrium of phosphate removal were investigated to evaluate the performance of modified alkaline residue. After treatment by NaOH (AR-NaOH), removal performance was significantly improved, while removal performance was almost completely lost after treatment by HCl (AR-HCl). The kinetics of the removal process by all adsorbents was well characterized by the pseudo second-order model. The Langmuir model exhibited the best correlation for AR-HCl, while AR was effectively described by Freundlich model. Both models were well fitted to AR-NaOH. The maximum adsorption capacities calculated from Langmuir equation were in following manner: AR-NaOH > AR > AR-HCl. Phosphate removal by alkaline residue was pH dependent process. Mechanisms for phosphate removal mainly involved adsorption and precipitation, varied with equilibrium pH of solution. For AR-HCl, the acid equilibrium pH (< 6.0) was unfavorable for the formation of Ca-P precipitate, with adsorption as the key mechanism for phosphate removal. In contrast, for AR and AR-NaOH, precipitation was the dominant mechanism for phosphate removal, due to the incrase on pH (> 8.0) after phosphate removal. The results of both XRD and SEM analysis confirmed CaHPO4·2H2O formation after phosphate removal by AR and AR-NaOH.


Asunto(s)
Fosfatos/química , Eliminación de Residuos Líquidos/métodos , Aguas Residuales/química , Adsorción , Residuos Industriales , Contaminantes Químicos del Agua/química , Purificación del Agua/métodos
12.
Environ Pollut ; 351: 123969, 2024 Jun 15.
Artículo en Inglés | MEDLINE | ID: mdl-38615835

RESUMEN

The issue of cadmium (Cd) contamination in alkaline soils is escalating, necessitating the prompt implementation of effective passivation strategies. Biochar has gained significant attention for its potential in immobilizing heavy metals; however, the suitability of biochar as a remediation material and its micro-scale interaction mechanisms with Cd under alkaline conditions remain unclear. Rape straw (RS) were pyrolyzed at 400 °C (RB400) and 700 °C (RB700) to produce biochar. Adsorption and soil incubation experiments were carried out to assess the feasibility of using rape straw derived biochar pyrolyze at different temperatures and understanding their remediation mechanisms in alkaline environments. The sorption capacity for Cd immobilization was evaluated using sorption isotherms, revealing that RB700 exhibited enhanced Cd sorption performance with a maximum sorption capacity of 119.33 mg g-1 calculated from the Langmuir isotherm equation at pH 8. Cd L3-edge X-ray absorption near-edge structure (XANES) spectroscopy analysis confirmed that the dominant sorption species of Cd were organic Cd in RB400, with CdCO3 precipitation increased to 73.9% in RB700. Solid-state 13C nuclear magnetic resonance (13C-NMR) spectroscopy demonstrated that aromatic and carboxyl C functional groups are involved in the organic sorption of Cd through complexation and Cd2+-π interactions in alkaline solutions. The precipitation of CdCO3 in RB700 may resulted in a more effective passivation effect compared to RB400, leading to a significant 15.54% reduction in the DTPA-Cd content in Cd-contaminated soil. These findings highlight the effective Cd passivation Cd in alkaline environments by rape straw derived biochar, providing new molecular insights into the Cd retention mechanism of biochar. Furthermore, it presents novel ideas for improving remediation approaches for alkaline Cd-contaminated soils.


Asunto(s)
Cadmio , Carbón Orgánico , Restauración y Remediación Ambiental , Contaminantes del Suelo , Suelo , Cadmio/química , Carbón Orgánico/química , Contaminantes del Suelo/química , Restauración y Remediación Ambiental/métodos , Adsorción , Suelo/química , Concentración de Iones de Hidrógeno , Brassica rapa/química
13.
Sci Total Environ ; 923: 171455, 2024 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-38438029

RESUMEN

Neonicotinoid (NEO) insecticides have been frequently detected in natural aquatic environments. Nevertheless, the distribution of NEOs in artificial environments is not clear. The Beijing-Hangzhou Grand Canal is the longest canal in the world. The northern Jiangsu segment of the Grand Canal was selected to study the spatiotemporal variation and source of eight NEOs in the canal water and assess their ecological and health risks. The total NEO concentration in the canal water was 12-289 ng L-1 in the dry season and 18-373 ng L-1 in the wet season, which were within the concentration range in other 11 natural rivers worldwide. The average total NEO concentrations were not statistically different between the seasons; only the concentrations of imidaclothiz, thiacloprid (THI), acetamiprid, and dinotefuran were different. At city scale, the total NEO concentration in the dry season showed a decreasing trend along the water flow from Xuzhou City to Yangzhou City. The total NEO concentrations were found to be positively correlated with the sown area of farm crops and the rural labour force, indicating the agricultural influence on the spatial distribution of NEO concentrations. In the wet season, relatively high NEO concentrations were distributed in downstream sites under the influence of artificial regulation. The primary contributor to the NEO inputs into the canal was the nonpoint source in the dry and wet seasons, with a relative contribution of 68 %. THI, imidacloprid, clothianidin and thiamethoxan would produce chronic ecological risks in both seasons. Further consideration needs to be given to the above four NEOs and NEO mixtures. The human health risks that NEOs posed by drinking water were assessed based on the chronic daily intake (CDI). The maximum CDI for adults and children was lower than the reference doses. This suggested public health would not be at risk from canal water consumption.


Asunto(s)
Insecticidas , Tiazinas , Adulto , Niño , Humanos , Insecticidas/análisis , Beijing , Neonicotinoides , Nitrocompuestos , Agua , Ríos , China
14.
Vet Res ; 44: 90, 2013 Oct 03.
Artículo en Inglés | MEDLINE | ID: mdl-24090087

RESUMEN

The intestinal nematode Baylisascaris schroederi is an important cause of death for wild and captive giant pandas. Inorganic pyrophosphatases (PPases) are critical for development and molting in nematode parasites and represent potential targets for vaccination. Here, a new PPase homologue, Bsc-PYP-1, from B. schroederi was identified and characterized, and its potential as a vaccine candidate was evaluated in a mouse challenge model. Sequence alignment of PPases from nematode parasites and other organisms show that Bsc-PYP-1 is a nematode-specific member of the family I soluble PPases. Immunohistochemistry revealed strong localization of native Bsc-PYP-1 to the body wall, gut epithelium, ovary and uterus of adult female worms. Additionally, Bsc-PYP-1 homologues were found in roundworms infecting humans (Ascaris lumbricoides), swine (Ascaris suum) and dogs (Toxocara canis). In two vaccine trials, recombinant Bsc-PYP-1 (rBsc-PYP-1) formulated with Freund complete adjuvant induced significantly high antigen-specific immunoglobulin (Ig)G but no IgE or IgM responses. Analysis of IgG-subclass profiles revealed a greater increase of IgG1 than IgG2a. Splenocytes from rBsc-PYP-1/FCA-immunized mice secreted low levels of T helper (Th)1-type cytokines, interferon-γ and interleukin (IL)-2, while producing significantly high levels of IL-10 and significantly elevated levels of IL-4 (Th2 cytokines) after stimulation with rBsc-PYP-1 in vitro. Finally, vaccinated mice had 69.02-71.15% reductions (in 2 experiments) in larval recovery 7 days post-challenge (dpc) and 80% survival at 80 dpc. These results suggest that Th2-mediated immunity elicited by rBsc-PYP-1 provides protection against B. schroederi, and the findings should contribute to further development of Bsc-PYP-1 as a candidate vaccine against baylisascariasis.


Asunto(s)
Antígenos Helmínticos/genética , Infecciones por Ascaridida/prevención & control , Ascaridoidea/genética , Ascaridoidea/inmunología , Proteínas del Helminto/genética , Pirofosfatasa Inorgánica/genética , Vacunas/inmunología , Secuencia de Aminoácidos , Animales , Antígenos Helmínticos/química , Antígenos Helmínticos/metabolismo , Infecciones por Ascaridida/inmunología , Infecciones por Ascaridida/parasitología , Ascaridoidea/metabolismo , Femenino , Proteínas del Helminto/química , Proteínas del Helminto/metabolismo , Pirofosfatasa Inorgánica/química , Pirofosfatasa Inorgánica/metabolismo , Ratones , Ratones Endogámicos BALB C , Datos de Secuencia Molecular , Filogenia , Reacción en Cadena de la Polimerasa , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Organismos Libres de Patógenos Específicos
15.
Environ Sci Pollut Res Int ; 30(2): 3997-4009, 2023 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-35963968

RESUMEN

Food processing of bean worm generates copious amount of skin as solid waste posing a serious environmental concern. The present study utilized bean worm skin (BWS) waste to produce KOH-modified biochars (KBWS-BCs) for the removal of sulfamethoxazole (SMX) from aqueous solution for the first time. Characterization of KBWS-BCs was systematically investigated via multiple instrumental analysis techniques. The sorption performance of KBWS-BCs as a function of solution pH, reaction time, initial SMX concentration, and reaction temperature was investigated using batch experiments. The classic kinetics and isotherm models were employed to fit the sorption data. KBWS-BCs exhibited large surface areas (3331-4742 m2 g-1) and ultrahigh sorption performance for SMX (maximum adsorption capacities of 909-2000 mg g-1), which were comparable to those of other modified biochars and even those of well-designed materials. Thermodynamic study indicated that the sorption of SMX on KBWS-BCs was a spontaneous (△G° < 0) and exothermic (△H° < 0) process. Mechanism analysis showed that both chemisorption and physisorption were responsible for the adsorption of SMX by KBWS-BCs. Overall, recycling BWS for preparation of high-performance biochars can be a "win-win" strategy for both disposal of BWS and removal of SMX from wastewater.


Asunto(s)
Sulfametoxazol , Contaminantes Químicos del Agua , Sulfametoxazol/química , Carbón Orgánico/química , Compuestos de Potasio , Contaminantes Químicos del Agua/análisis , Adsorción
16.
Front Cell Infect Microbiol ; 13: 1091825, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-36743312

RESUMEN

Non-small cell lung cancer (NSCLC) is the predominant form of lung cancer and is one of the most fatal cancers worldwide. Recently, the International Association for the Study of Lung Cancer (IASLC) proposed a novel grading system based on the predominant and high-grade histological patterns for invasive pulmonary adenocarcinoma (IPA). To improve outcomes for NSCLC patients, we combined serum metabolomics and fecal microbiology to screen biomarkers in patients with early-stage NSCLC and identified characteristic microbial profiles in patients with different grades of IPA. 26 genera and 123 metabolites were significantly altered in the early-stage NSCLC patients. Agathobacter, Blautia, Clostridium, and Muribaculacea were more abundant in the early-stage NSCLC patients compared with healthy controls. For the different grades of IPA, the characteristic microorganisms are as follows: Blautia and Marinobacter in IPA grade type 1; Dorea in IPA grade type 2; and Agathobacter in IPA grade type 3. In the metabolome results, the early-stage NSCLC group mainly included higher levels of sphingolipids (D-erythro-sphingosine 1-phosphate, palmitoyl sphingomyelin), fatty acyl (Avocadyne 1-acetate, 12(S)-HETE, 20-Carboxy-Leukotriene B4, Thromboxane B3, 6-Keto-prostaglandin f1alpha, Sebacic acid, Tetradecanedioic acid) and glycerophospholipids (LPC 20:2, LPC 18:0, LPC 18:4, LPE 20:2, LPC 20:1, LPC 16:1, LPC 20:0, LPA 18:2, LPC 17:1, LPC 17:2, LPC 19:0). Dysregulation of pathways, such as sphingolipid metabolism and sphingolipid signaling pathway may become an emerging therapeutic strategy for early-NSCLC. Correlation analysis showed that gut microbiota and serum metabolic profiles were closely related, while Muribaculacea and Clostridium were the core genera. These findings provide new biomarkers for the diagnosis of early-stage NSCLC and the precise grading assessment of prognostic-related IPAs, which are of clinical importance and warrant further investigation of the underlying molecular mechanisms.


Asunto(s)
Carcinoma de Pulmón de Células no Pequeñas , Microbioma Gastrointestinal , Neoplasias Pulmonares , Humanos , Carcinoma de Pulmón de Células no Pequeñas/patología , Biomarcadores , Metabolómica/métodos , Esfingolípidos
17.
Biotechnol Genet Eng Rev ; : 1-21, 2023 Apr 17.
Artículo en Inglés | MEDLINE | ID: mdl-37066722

RESUMEN

The score and prognostic value of necroptosis were analyzed in the TCGA and GSE120622 datasets. Necroptosis has the highest correlation with the immune microenvironment, and the high score in NSCLC correlates with poor prognosis. Differentially expressed genes between non-small cell lung cancer (NSCLC) and controls in both datasets were identified and subjected to construct co-expression networks, respectively. Black and blue modules were selected because of high correction with necroptosis. The intersected two module genes were mainly involved in immune and inflammatory response, cell cycle process and DNA replication. Nine marker genes of necroptosis were identified in these modules and considered as candidate genes. Based on candidate genes, we identified two clusters utilizing concordance clustering, additionally dividing NSCLC samples into high- and low-risk groups. There were significant differences in overall survival between two clusters and between high- and low-risk groups. Furthermore, PARP1 was found among the candidate genes to be the target gene of dexmedetomidine acting on necroptosis. Molecular experimental results found that PARP1 was highly expressed in the dexmedetomidine treated NSCLC compared with the NSCLC. Candidate genes associated with necroptosis may provide a powerful prognostic tool for precision oncology. Dexmedetomidine may target PARP1 to promote necroptosis and then affect NSCLC.

18.
Environ Pollut ; 333: 122068, 2023 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-37330189

RESUMEN

Neonicotinoids (NEOs) are the most widely used insecticides in the world. Nevertheless, the occurrence and distribution of NEOs in agricultural areas are not well understood. This study investigated the concentration, sources, ecological risks, and health risks of eight NEOs in the water of the Huai River, which flows through a typical agricultural area in China. The total concentration of NEOs in the river water ranged from 1.02 to 191.2 ng L-1, with an average of 64.1 ng L-1. Thiamethoxam was the dominant compound, with an average relative contribution of 42.5%. The average concentration of the total NEOs in downstream was significantly higher than that in upstream (p < 0.05). This may be related to the intensity of agricultural activities. The riverine NEO fluxes increased by approximately 12 times from the upper site to the lower site. More than 1.3 tons of NEOs in 2022 were transferred into Lake Hongze, the largest regulative lake on the Eastern Route of the South-to-North Water Diversion Project. Nonpoint sources were the major contributor to the total NEO inputs, and water use was the main output pathway. The risk assessment indicated that the individual NEOs in the river water presented low ecological risks. The NEO mixtures would produce chronic risks to aquatic invertebrates in 50% of the sampling sites, which were mostly distributed in downstream. Thus, more attention should be given to the downstream. Based on the Monte Carlo simulation, the health risks of NEOs via water consumption were estimated. The maximum chronic daily intakes were 8.4 × 10-4, 2.25 × 10-4, 1.27 × 10-4, 1.88 × 10-4 mg kg-1 day-1 for boys, girls, men, and women, respectively, which were approximately 2 orders of magnitude lower than the acceptable daily intake. Therefore, river water consumption would not be a concern for the public health.

19.
Waste Manag ; 169: 52-61, 2023 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-37406504

RESUMEN

Poor properties and high concentrations of heavy metals are still major concerns of successful application of animal manure-derived biochar into the environment. This work thus proposed to add chlorine-based additives (Cl-additives, i.e., CaCl2, MgCl2, KCl, NaCl, and PVC, 50 g Cl/ kg) to improve biochar properties and enhance heavy metal volatilization during swine manure pyrolysis. The results showed that the addition of CaCl2 could improve the retention of carbon (C) by up to 13.1% during pyrolysis, whereas other Cl-additives had little effect on it. Moreover, CaCl2 could enhance the aromaticity of biochar, as indicated by lower H/C ratio than raw biochar. Pretreatment with CaCl2, MgCl2 and PVC reduced phosphorus (P) solubility but increased its bioavailability via the formation of chlorapatite (Ca5(PO4)3Cl). The CaCl2 was more effective for enhancing the volatilization efficiency of heavy metals than other Cl-additives, except for Pb that tended to react with the generated Ca5(PO4)3Cl to form more stable and less volatile Pb5(PO4)3Cl. However, high pyrolysis temperature (900℃) was essential for CaCl2 to simultaneously decrease the bioavailability of heavy metals. Our results indicated that co-pyrolysis of swine manure with CaCl2 is a promising strategy to increase C retention, P bioavailability, and volatilization of heavy metals, and, at higher temperature, reduce the bioavailability of biochar-born heavy metals.


Asunto(s)
Cloro , Metales Pesados , Porcinos , Animales , Estiércol , Pirólisis , Cloruro de Calcio , Plomo , Volatilización , Carbón Orgánico
20.
J Hazard Mater ; 440: 129789, 2022 10 15.
Artículo en Inglés | MEDLINE | ID: mdl-36007365

RESUMEN

Activated carbon and metal organic frameworks have been tested as NH3 recovery adsorbents, however, they are limited due to low NH3 adsorption capacity and high cost, respectively. In this study, ethylene glycol dimethacrylate (EGDMA) polymers as the representative ester plastics were tested, and their structure and adsorption sites were regulated using HNO3, HCl, or H2SO4 with varied H+ concentrations. The results showed that the EGDMA polymers all used hydrolysis which promoted NH3 adsorption via different mechanisms. With HNO3 and HCl optimization, an increased surface area promoted NH3 adsorption via physical forces. H2SO4 optimization resulted in -COOH, -OH, and -SO3H formation, which reacted with NH3 by chemical adsorption and hydrogen bonds. This significantly increased the NH3 adsorption capacity (85.99 mg·g-1) compared to the material before optimization (0.36 mg·g-1). This study presents a novel low-cost and efficient method to recycle waste plastics as NH3 adsorbents.


Asunto(s)
Amoníaco , Estructuras Metalorgánicas , Adsorción , Amoníaco/química , Carbón Orgánico/química , Ésteres , Metacrilatos , Plásticos
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