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1.
Biochem Biophys Res Commun ; 730: 150391, 2024 10 20.
Artículo en Inglés | MEDLINE | ID: mdl-39002199

RESUMEN

Glucocorticoid-induced osteoporosis serves as a primary cause for secondary osteoporosis and fragility fractures, representing the most prevalent adverse reaction associated with prolonged glucocorticoid use. In this study, to elucidate the impact and underlying mechanisms of fluid shear stress (FSS)-mediated Piezo1 on dexamethasone (Dex)-induced apoptosis, we respectively applied Dex treatment for 6 h, FSS at 9 dyne/cm2 for 30 min, Yoda1 treatment for 2 h, and Piezo1 siRNA transfection to intervene in MLO-Y4 osteocytes. Western blot analysis was used to assess the expression of Cleaved Caspase-3, Bax, Bcl-2, and proteins associated with the PI3K/Akt pathway. Additionally, qRT-PCR was utilized to quantify the mRNA expression levels of these molecules. Hoechst 33258 staining and flow cytometry were utilized to evaluate the apoptosis levels. The results indicate that FSS at 9 dyne/cm2 for 30 min significantly upregulates Piezo1 in osteocytes. Following Dex-induced apoptosis, the phosphorylation levels of PI3K and Akt are markedly suppressed. FSS-mediated Piezo1 exerts a protective effect against Dex-induced apoptosis by activating the PI3K/Akt pathway. Additionally, downregulating the expression of Piezo1 in osteocytes using siRNA exacerbates Dex-induced apoptosis. To further demonstrate the role of the PI3K/Akt signaling pathway, after intervention with the PI3K pathway inhibitor, the activation of the PI3K/Akt pathway by FSS-mediated Piezo1 in osteocytes was significantly inhibited, reversing the anti-apoptotic effect. This study indicates that under FSS, Piezo1 in MLO-Y4 osteocytes is significantly upregulated, providing protection against Dex-induced apoptosis through the activation of the PI3K/Akt pathway.


Asunto(s)
Apoptosis , Dexametasona , Canales Iónicos , Osteocitos , Fosfatidilinositol 3-Quinasas , Proteínas Proto-Oncogénicas c-akt , Transducción de Señal , Estrés Mecánico , Osteocitos/metabolismo , Osteocitos/efectos de los fármacos , Apoptosis/efectos de los fármacos , Proteínas Proto-Oncogénicas c-akt/metabolismo , Fosfatidilinositol 3-Quinasas/metabolismo , Animales , Ratones , Canales Iónicos/metabolismo , Canales Iónicos/genética , Transducción de Señal/efectos de los fármacos , Dexametasona/farmacología , Línea Celular
2.
Mol Cell Biochem ; 478(3): 597-608, 2023 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-35980563

RESUMEN

A list of microRNAs (miRs) has been referred to involve in the development of hypoxic-ischemic brain damage (HIBD). Based on that, we probed the concrete role of miR-214-3p regulating thioredoxin-interacting protein (TXNIP) in the illness. A neonatal HIBD mouse model was established using the Rice-Vannucci method, followed by measurements of miR-214-3p and TXNIP levels in brain tissues. After modeling, mice were given brain injection of the compounds that could alter miR-214-3p and TXNIP expression. Afterward, neurological function, neuronal inflammation, neuronal apoptosis, neuron morphology, and the number of Nissl body were assessed in HIBD mice. The binding of miR-214-3p to TXNIP was analyzed. Lower miR-214-3p and higher TXNIP were analyzed in brain tissues of mice with HIBD. Up-regulating miR-214-3p or depleting TXNIP improved neurological function, reduced neuronal inflammation and neuronal apoptosis, attenuated morphological damage of neurons, and increased the number of Nissl bodies in mice with HIBD. TXNIP was targeted by miR-214-3p and overexpressing TXNIP reversed the therapeutic effect of miR-214-3p on HIBD mice. It is noted that promotion of miR-214-3p relieves HIBD in mice through inhibiting TXNIP expression.


Asunto(s)
Hipoxia-Isquemia Encefálica , MicroARNs , Animales , Ratones , Hipoxia-Isquemia Encefálica/metabolismo , MicroARNs/metabolismo , Encéfalo/metabolismo , Apoptosis , Animales Recién Nacidos , Proteínas Portadoras/metabolismo , Tiorredoxinas/metabolismo
3.
Int J Mol Sci ; 24(2)2023 Jan 07.
Artículo en Inglés | MEDLINE | ID: mdl-36674716

RESUMEN

The demand for economic benefits has led to an increase in the proportion of high-concentrate (HC) feed in the ruminant diet, resulting in an increased incidence of subacute ruminal acidosis (SARA). During SARA, a high concentration of lipopolysaccharide (LPS) translocated in the rumen induces a systemic inflammatory response. Inflammatory diseases, such as endometritis and mastitis, are often associated with SARA; however, in sheep, the mechanism of the effect of SARA on the endometrium has rarely been reported. Therefore, the aim of this study was to investigate, for the first time, the influence of LPS translocation on endometrial tight junctions (TJs) during SARA in sheep. The results showed that LPS and TNFα levels in the ruminal fluid, serum, and endometrial tissue supernatant during SARA increased, transcription levels of TLR4, NFκB, and TNFα in the endometrium increased, the protein expression level of claudin-1 in the endometrium increased, and the protein expression level of occludin decreased. 17ß-estradiol (E2) inhibits claudin-1 protein expression and promotes occludin expression, and progesterone (P4) promotes claudin-1 protein expression and inhibits occludin protein expression. E2 and P4 regulate claudin-1 and occludin protein expression through their receptor pathways. Here, we found that LPS hindered the regulatory effect of E2 and P4 on endometrial TJs by inhibiting their receptor expression. The results of this study indicate that HC feeding can cause SARA-induced LPS translocation in sheep, increase susceptibility to systemic inflammation, induce the endometrial inflammatory response, and cause endometrial epithelial TJ damage directly and/or by obstructing E2 and P4 function. LPS translocation caused by SARA has also been suggested to induce an endometrial inflammatory response, resulting in endometrial epithelial barrier damage and physiological dysfunction, which seriously affects ruminant production. Therefore, this study provides new evidence that SARA is a potential factor that induces systemic inflammation in ruminants. It provides theoretical support for research on the prevention of endometritis in ruminants.


Asunto(s)
Acidosis , Endometritis , Femenino , Humanos , Ovinos , Animales , Factor de Necrosis Tumoral alfa/genética , Factor de Necrosis Tumoral alfa/metabolismo , Rumen , Endometritis/veterinaria , Endometritis/metabolismo , Lipopolisacáridos/metabolismo , Claudina-1/metabolismo , Ocludina/metabolismo , Dieta/veterinaria , Inflamación/metabolismo , Endometrio/metabolismo , Acidosis/metabolismo , Concentración de Iones de Hidrógeno
4.
Brain Behav Immun ; 87: 890-897, 2020 07.
Artículo en Inglés | MEDLINE | ID: mdl-31931152

RESUMEN

Parkinson's disease (PD) is one of the most significant medical and social burdens of our time. The prevalence of PD increases with age and the number of individuals diagnosed with PD is expected to double from 6.9 million in 2015 to 14.2 million in 2040. To date, no drugs can stop the ongoing neurodegeneration caused by PD due to its unclear and complex pathogenic mechanisms. It has been wildly recognized that both gut microbiota and neuro-immunity are involved in the pathology of PD. In this review, we intend to provide a comprehensive overview of current knowledge on how gut microbiota involved in immune-driven pathogenesis of PD, and its potential as a new target of dietary and/or therapeutic interventions for PD.


Asunto(s)
Microbioma Gastrointestinal , Enfermedad de Parkinson , Humanos
5.
J Neurovirol ; 25(1): 127-132, 2019 02.
Artículo en Inglés | MEDLINE | ID: mdl-30397825

RESUMEN

Vibrio vulnificus usually causes wound infection, gastroenteritis, and septicemia. However, it is a rare conditional pathogen causing meningoencephalitis. We report a case of a young, immunocompromised man presenting with severe sepsis after exposure to sea water and consumption of seafood. The patient subsequently developed meningoencephalitis, and Vibrio vulnificus was isolated from his blood culture. The sequence was confirmed by Next-generation sequencing of a sample of cerebrospinal fluid, as well as from a bacteria culture. After the pathogen was detected, the patient was treated with ceftriaxone, doxycycline, and moxifloxacin for 6 weeks, which controlled his infection. In this case, we acquired his clinical and dynamic MRI presentations, which were never reported. Physicians should consider Vibrio vulnificus infections when they see a similar clinical course, brain CT and MRI findings, susceptibility factors and recent seafood ingestion or exposure to seawater. Due to high mortality, the early diagnosis and treatment of Vibrio vulnificus infections are crucial. Next-generation sequencing was found to be useful for diagnosis.


Asunto(s)
Antibacterianos/uso terapéutico , Huésped Inmunocomprometido , Meningoencefalitis/inmunología , Sepsis/inmunología , Vibrio vulnificus/patogenicidad , Adulto , Ceftriaxona/uso terapéutico , Doxiciclina/uso terapéutico , Humanos , Imagen por Resonancia Magnética , Masculino , Meningoencefalitis/diagnóstico por imagen , Meningoencefalitis/tratamiento farmacológico , Meningoencefalitis/microbiología , Moxifloxacino/uso terapéutico , Alimentos Marinos/microbiología , Agua de Mar/microbiología , Sepsis/diagnóstico por imagen , Sepsis/tratamiento farmacológico , Sepsis/microbiología , Esplenectomía , Talasemia/inmunología , Talasemia/patología , Talasemia/cirugía , Resultado del Tratamiento , Vibrio vulnificus/efectos de los fármacos , Vibrio vulnificus/crecimiento & desarrollo , Vibrio vulnificus/aislamiento & purificación
6.
BMC Neurol ; 18(1): 174, 2018 Oct 20.
Artículo en Inglés | MEDLINE | ID: mdl-30342480

RESUMEN

BACKGROUND: DNA methyltransferase 1 (EC 2.1.1.37), encoded by DNMT1 gene, is one of key enzymes in maintaining DNA methylation patterns of the human genome. It plays a crucial role in embryonic development, imprinting and genome stability, cell differentiation. The dysfunction of this group of enzymes can lead to a variety of human genetic disorders. Until now, mutations in DNMT1 have been found to be associated with two distinct phenotypes. Mutations in exon 20 of this gene leads to hereditary sensory and autonomic neuropathy type IE, and mutations in exon 21 cause autosomal dominant cerebellar ataxia, deafness and narcolepsy. CASE PRESENTATION: Here we report a novel DNMT1 mutation in a sporadic case of a Chinese patient with cerebellar ataxia, multiple motor and sensory neuropathy, hearing loss and psychiatric manifestations. Furthermore, we elucidated its pathogenic effect through molecular genetics studies and revealed that this defective DNMT1 function is responsible for the phenotypes in this individual. CONCLUSION: Our findings expand the spectrum of DNMT1-related disorders and provide a good example of precision medicine through the combination of exome sequencing and clinical testing.


Asunto(s)
ADN (Citosina-5-)-Metiltransferasa 1/genética , Neuropatías Hereditarias Sensoriales y Autónomas/genética , Adulto , Ataxia Cerebelosa/genética , Metilación de ADN , Exones , Femenino , Humanos , Mutación , Fenotipo
7.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 38(10): 1276-1282, 2024 Oct 15.
Artículo en Zh | MEDLINE | ID: mdl-39433504

RESUMEN

Objective: To review research progress on femoral attachment positioning during medial patellofemoral ligament (MPFL) reconstruction, so as to provide a reference for accurate positioning in clinic. Methods: The literature at home and abroad on femoral attachment positioning during MPFL reconstruction was extensively reviewed and summarized. Results: MPFL is the main ligament that restricts patellar outward migration, so MPFL reconstruction is the main treatment for patellar dislocation, but the accuracy of intraoperative femoral attachment positioning will significantly affect the effectiveness. At present, there are three main methods for femoral attachment positioning in MPFL reconstruction, including imaging positioning, bony landmark positioning, and new technology. Among them, the main imaging positioning method is the "Schöttle point" method, but it has high requirements for fluoroscopic positioning, and can only be accurately positioned under standard lateral fluoroscopy of the femur. The bony landmark positioning method mainly locates the femoral attachment by touching or dissecting the bony landmarks such as adductor tubercles and medial epicondyle of femur, but its disadvantages are that the positioning is not accurate enough, the intraoperative visual field exposure requirements are high, and a large incision is required. In order to avoid the problem that the simple bony landmark positioning method, in recent years, the combination of bony landmarks combined with arthroscopy, three-dimensional (3D) printing technology, and robot-assisted positioning methods have begun to be used in clinical practice. New technology localization methods have shown good results by preparing guides before operation, planning positioning paths in advance, or directly using robots to assist positioning during operation. Conclusion: The accurate positioning of the femoral attachment in MPFL reconstruction is crucial, and the method of accurate and rapid intraoperative determination needs to be further improved and optimized. In the future, it is expected that the combination of computer image recognition correction technology and intraoperative position assistance will solve this problem.


Asunto(s)
Fémur , Articulación Patelofemoral , Procedimientos de Cirugía Plástica , Humanos , Fémur/cirugía , Procedimientos de Cirugía Plástica/métodos , Articulación Patelofemoral/cirugía , Luxación de la Rótula/cirugía , Ligamento Rotuliano/cirugía , Ligamentos Articulares/cirugía , Fluoroscopía
8.
Org Lett ; 26(31): 6647-6651, 2024 Aug 09.
Artículo en Inglés | MEDLINE | ID: mdl-39073919

RESUMEN

Readily accessible N-sulfonylaminophthalimides were developed to be efficient sulfonyl radical precursors upon being treated with a base/oxidant under mild conditions. The method was applied to the oxysulfonylation of olefins, providing ß-ketosulfones and isobenzofurans stereoselectively. On the basis of control experiments, density functional theory calculations, and the literature, a plausible radical mechanism was proposed. The findings offered a chance to develop novel radical precursors based on diversely substituted N-aminophthalimides, which might establish a universal mild approach for the generation of various radicals.

9.
Phytomedicine ; 128: 155468, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38471315

RESUMEN

BACKGROUND: Oxidative stress is considered the main cause of granulosa cell apoptosis in ovarian disease. Curcumin has various biological roles, but its potential role in protecting granulosa cells from oxidative damage remains unidentified. PURPOSE: The study revealed the protective effect of curcumin on granulosa cell survival under oxidative stress, and explored its mode of action. STUDY DESIGN: The protective effect of curcumin on oxidative stress-induced ovarian cell apoptosis was evaluated in vivo and in vitro, and the role of autophagy and AMPK/mTOR signaling pathway in this process was also demonstrated. METHODS: First, mice were injected to 3-nitropropionic acid (3-NPA, 20 mg/kg/day) for 14 consecutive days to establish the ovarian oxidative stress model, at same time, curcumin (50, 100, 200 mg/kg/day) was given orally. Thereafter, functional changes, cell apoptosis, and autophagy in ovarian tissue were evaluated by hematoxylin-eosin staining, enzyme-linked immunosorbent assay, western blotting, TUNEL assays, and transmission electron microscopy. Finally, oxidative stress model of granulosa cells was established with H2O2in vitro and treated with curcumin. The underlying mechanisms of curcumin to protect the apoptosis under oxidative stress in vitro were determined using western blotting and TUNEL assays. RESULTS: In our study, after curcumin treatment, the mouse ovarian function disorder under 3-nitropropionic acid-induced oxidative stress recovered significantly, and ovarian cell apoptosis decreased. H2O2 induced granulosa cell apoptosis in vitro, and curcumin antagonized this process. Autophagy contributes to tissue and cell survival under stress. We therefore examined the role of autophagy in this process. According to the in vivo and in vitro results, curcumin restored autophagy under oxidative stress. The autophagy inhibitor (chloroquine) exhibited the same effect as curcumin, whereas the autophagy activator (rapamycin) antagonized the effect of curcumin. In addition, the study found that the AMPK/mTOR pathway plays a crucial role in curcumin- mediated autophagy to protect against oxidative stress-induced apoptosis. CONCLUSION: Our findings for the first time systematically revealed a new mechanism through which curcumin protects ovarian granulosa cells from oxidative stress-induced damage through AMPK/mTOR-mediated autophagy and suggested that it can be a new therapeutic direction for female ovarian diseases.


Asunto(s)
Autofagia , Curcumina , Ovario , Estrés Oxidativo , Serina-Treonina Quinasas TOR , Animales , Femenino , Ratones , Proteínas Quinasas Activadas por AMP/efectos de los fármacos , Proteínas Quinasas Activadas por AMP/metabolismo , Apoptosis/efectos de los fármacos , Autofagia/efectos de los fármacos , Curcumina/farmacología , Células de la Granulosa/efectos de los fármacos , Peróxido de Hidrógeno/toxicidad , Nitrocompuestos , Ovario/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Propionatos/farmacología , Transducción de Señal/efectos de los fármacos , Serina-Treonina Quinasas TOR/efectos de los fármacos , Serina-Treonina Quinasas TOR/metabolismo
10.
Oxid Med Cell Longev ; 2022: 9173514, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35509839

RESUMEN

The pathogenesis of Parkinson's disease remains unclear that there is no cure for Parkinson's disease yet. The abnormal expressions of certain miRNA are closely related to the occurrence and progression of Parkinson's disease. Here, we demonstrate that miR-9-5p inhibits the dopaminergic neuron apoptosis via the regulation of ß-catenin signaling which directly targets SCRIB, a tumor suppressor gene. Besides, miR-9-5p improved the motor function of mice with Parkinson's disease. The results of this study suggest that miR-9-5p might be a potential therapeutic target against Parkinson's disease.


Asunto(s)
MicroARNs , Enfermedad de Parkinson , Animales , Apoptosis , Línea Celular Tumoral , Proliferación Celular/genética , Neuronas Dopaminérgicas/metabolismo , Metaloproteinasas de la Matriz , Ratones , MicroARNs/metabolismo , Enfermedad de Parkinson/genética , Enfermedad de Parkinson/metabolismo , beta Catenina/metabolismo
11.
Sci Total Environ ; 842: 156952, 2022 Oct 10.
Artículo en Inglés | MEDLINE | ID: mdl-35752240

RESUMEN

The effect of long-term water and integrated fertilization on prokaryotic microorganisms and their regulation for crop nutrient uptake remains unknown. Therefore, the impact of soil water and integrated fertilization after eight years on prokaryotic microbial communities in different compartments of root zone and their association with wheat nitrogen (N) absorption and yield were investigated. The results showed that compared with fertilization treatments (F), water regimes (W) more drastically modulated the prokaryotic microbial community structure and diversity in bulk soil, rhizosphere and endosphere. The increase of irrigation improved the prokaryotic diversity in the rhizosphere and endosphere while decreased the diversity in the bulk soil. Application of organic fertilizers significantly improved soil organic matter (SOM) and nutrient contents, increased rhizosphere and endophytic prokaryotic microbial diversity, and elevated the relative abundance of aerobic ammonia oxidation and nitrification-related functional microorganisms in rhizosphere and endosphere. Increasing irrigation elevated the relative abundance of functional microorganisms related to aerobic ammonia oxidation and nitrification in the rhizosphere and endosphere. Soil water content (SWC) and NH4+-N as well as NO3--N were key predictors of prokaryotic microbial community composition under W and F treatments, respectively. Appropriate application of irrigation and organic fertilizers increased the relative abundance of some beneficial bacteria such as Flavobacterium. Water and fertilization treatments regulated the prokaryotic microbial communities of bulk soil, rhizosphere and endosphere by altering SWC and SOM, and provided evidence for the modulation of prokaryotic microorganisms to promote nitrogen uptake and wheat yield under long-term irrigation and fertilization. Conclusively, the addition of organic manure (50 %) with inorganic fertilizers (50 %) and reduced amount of irrigation (pre-sowing and jointing-period irrigation) decreased the application amount of chemical fertilizers and water, while increased SOM and nutrient content, improved prokaryotic diversity, and changed prokaryotic microbial community structure in the wheat root zone, resulting in enhanced nutrient uptake and wheat yield.


Asunto(s)
Fertilizantes , Microbiota , Fertilización , Fertilizantes/análisis , Nitrógeno , Suelo/química , Microbiología del Suelo , Triticum , Agua
12.
Org Lett ; 24(15): 2950-2954, 2022 04 22.
Artículo en Inglés | MEDLINE | ID: mdl-35416678

RESUMEN

Formal intramolecular 1,3-OH migration of α-imino carbene was achieved producing a unique zwitterion, and the subsequent selective annulation afforded α-amino cyclobutanone. Features such as readily available substrates, mild reaction conditions, a time-saving procedure, excellent functional group compatibility, and valuable transformations of the products qualified this unique protocol as an efficient tool for the synthesis of strained cyclic compounds. Density functional theory calculations were in good agreement with experimental observations, and a plausible mechanism is presented.


Asunto(s)
Rodio , Catálisis , Metano/análogos & derivados , Metano/química , Rodio/química
13.
Oxid Med Cell Longev ; 2022: 9611362, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35295717

RESUMEN

Atherosclerosis is a chronic inflammatory disease with a high prevalence worldwide, contributing to a series of adverse cardiovascular and cerebrovascular diseases. Periodontal disease induced by pathogenic periodontal microbiota has been well established as an independent factor of atherosclerosis. Periodontal microorganisms have been detected in atherosclerotic plaques. The high-risk microbiota dwelling in the subgingival pocket can stimulate local and systematic host immune responses and inflammatory cascade reactions through various signaling pathways, resulting in the development and progression of atherosclerosis. One often-discussed pathway is the Toll-like receptor-nuclear factor-κB (TLR-NF-κB) signaling pathway that plays a central role in the transduction of inflammatory mediators and the release of proinflammatory cytokines. This narrative review is aimed at summarizing and updating the latest literature on the association between periodontopathic microbiota and atherosclerosis and providing possible therapeutic ideas for clinicians regarding atherosclerosis prevention and treatment.


Asunto(s)
Aterosclerosis/fisiopatología , Inflamación/metabolismo , Microbiota/inmunología , Enfermedades Periodontales/microbiología , Adulto , Anciano , Animales , Femenino , Humanos , Masculino , Ratones , Persona de Mediana Edad , Factores de Riesgo , Receptor Toll-Like 4/metabolismo
14.
Cereb Circ Cogn Behav ; 2: 100005, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-36324728

RESUMEN

Objective: This study used a 3.0T high-resolution magnetic resonance imaging approach to explore basilar artery plaque characteristics in patients suffering from acute isolated pontine infarction. Materials and methods: 30 consecutive patients suffering from acute isolated pontine infarction were enrolled in this study and underwent examinations including high-resolution MRI assessment of the basilar artery within 7 days following infarction. Results: The basilar artery plaque burden of 16 patients with paramedian pontine infarction was 0.26±0.085, while the reconstruction index and enhancement rate index values in these patients were 1.097±0.133 and 1.750±0.447, respectively. In the 14 patients suffering from deep pontine infarction, these three values were 0.21±0.055, 0.896±0.223, and 1.285±0.611, respectively.These values differed significantly when comparing patients suffering from paramedian pontine infarction to those suffering from deep pontine infarction. Conclusion: This study suggests that the characteristics of basilar artery plaques differ between the two subtypes of pontine infarctions, which may account for the differences in prognosis associated with these two infarct subtypes.

15.
Psychiatr Genet ; 31(4): 135-139, 2021 08 01.
Artículo en Inglés | MEDLINE | ID: mdl-34133408

RESUMEN

POGZ is located on chromosome 1q21.3, encoding a pogo transposable element-derived protein with a zinc finger cluster. White-Sutton syndrome (WHSUS, OMIM:616364) is a genetic disorder resulting from de novo heterozygous pathogenic variants in POGZ, which manifests as intellectual disability, autism spectrum disorder, specific facial features and other phenotypic spectra. To date, a total of twenty-one de novo POGZ mutations in WHSUS have been reported. Here we report the identification of a novel missense variant in the coding region of the POGZ gene (c.4042G>C), which occurred in a 15-year-old male and his mother with WHSUS. We describe their clinical features and compare them with clinical data of patients with WHSUS from the literature. Our finding broadens the spectrum of POGZ mutations and provides a good example of precision medicine through the combination of exome sequencing and clinical testing.


Asunto(s)
Trastorno del Espectro Autista , Discapacidad Intelectual , Adolescente , Heterocigoto , Humanos , Discapacidad Intelectual/genética , Masculino , Mutación/genética , Mutación Missense/genética , Fenotipo , Transposasas/genética
16.
Bioengineered ; 12(1): 3348-3357, 2021 12.
Artículo en Inglés | MEDLINE | ID: mdl-34224319

RESUMEN

To investigate the effect of miR-149-5p on sphingosine-1-phosphate receptor 2 (S1PR2) expression level and contents of matrix metalloproteinase (MMP-9) and superoxide dismutase (SOD) in the pericytes after acute cerebral ischemia reperfusion in rats, so as to clarify the neuroprotective molecular mechanism induced by miR-149-5p and provide references for the treatment of neurological diseases, 60 male SD rats aged 7-8 weeks were selected and divided randomly into test group (establishing middle cerebral artery occlusion (MCAO) model) and control group (no modeling). Rat pericytes and peripheral cerebral infarction tissues were collected 12 h, 1 d, 3 d, 5 d, and 7 d after MCAO modeling, respectively. The pericytes were identified by immunofluorescence assay (IFA) and transfected with miR-149-5p. Fluorescence quantitative PCR (FQPCR) and Western blot were adopted to detect S1PR2 expression level. The expression of S1PR2 in MCAO model rats was detected by IFA. Immunohistochemistry (IHC) and quantitative real-time PCR (qRT-PCR) were used to detect the changes of MMP9 protein and mRNA levels of SOD1, SOD2, and SOD3 in brain tissue. The results showed that mRNA level and protein expression level of S1PR2 in the test group were higher than those in the control group three days after MCAO modeling (P < 0.05); the expression of S1PR2 increased 12 h after MCAO modeling and returned to the normal level on the 5th day, and the content of MMP9 protein in brain tissue of the test group was significantly lower than that of the control group (P < 0.05); the mRNA levels and SODs activity of SOD1, SOD2, and SOD3 in the test group were higher than those in the control group (P < 0.05). Therefore, miR-149-5p played a neuroprotective role by regulating S1PR2 to change the expression levels of SODS and MMP9.


Asunto(s)
Isquemia Encefálica/metabolismo , MicroARNs/metabolismo , Neuroprotección/genética , Pericitos/metabolismo , Receptores de Esfingosina-1-Fosfato/metabolismo , Animales , Encéfalo/metabolismo , Encéfalo/patología , Isquemia Encefálica/patología , Infarto de la Arteria Cerebral Media , Masculino , Metaloproteinasa 9 de la Matriz/genética , Metaloproteinasa 9 de la Matriz/metabolismo , MicroARNs/genética , Ratas , Ratas Sprague-Dawley , Daño por Reperfusión/metabolismo , Daño por Reperfusión/patología , Receptores de Esfingosina-1-Fosfato/genética
17.
Bioengineered ; 12(1): 3957-3967, 2021 12.
Artículo en Inglés | MEDLINE | ID: mdl-34288796

RESUMEN

To investigate mechanism of pericytes in the early stage of subarachnoid haemorrhage (SAH) and its associated microvascular spasm and neurovascular injury, 100 healthy 8-week-old Sprague-Dawley male rats were taken as subjects and divided into four groups: group A (sham operation, control group), group B (SAH operation group), group C (SAH operation group treated with scutellarin), and group D (SAH operation group treated with L-nitro-arginine). 72 hours after the operation, the rats were conducted assessment of neurological impairment, observation of microangiography, detection of blood-brain barrier permeability, observation of skull base haemorrhage, identification of pericyte culture, and measurement of blood nitric oxide. The results showed that neurological impairment score, degree of micro-vasoconstriction, and BBB permeability of group C were significantly better than those of group B and D (P<0.05), there was no significant difference between group C and group A (P>0.05). There were significantly fewer blood clots in the brain of group C, and the order of expression levels of α-smooth muscle actin (α-SMA) in perioperative cells of the four groups from highest to lowest were D, B, C, and A. Nitric oxide concentration inhibited expression of α-SMA in pericytes after SAH at both protein and mRNA levels. The detection results of nitric oxide in the blood of four groups of rats confirmed that pericyte phenotype conversion and actin α-SMA expression could be prevented by upregulation of nitric oxide in serum, so as to relieve pathological symptoms after SAH operation.


Asunto(s)
Encéfalo/patología , Pericitos/patología , Hemorragia Subaracnoidea/complicaciones , Vasoespasmo Intracraneal/etiología , Vasoespasmo Intracraneal/patología , Actinas/metabolismo , Angiografía , Animales , Barrera Hematoencefálica/patología , Encéfalo/diagnóstico por imagen , Diferenciación Celular , Fluorescencia , Óxido Nítrico/metabolismo , Pericitos/metabolismo , Permeabilidad , ARN Mensajero/genética , ARN Mensajero/metabolismo , Ratas Sprague-Dawley , Hemorragia Subaracnoidea/diagnóstico por imagen , Hemorragia Subaracnoidea/fisiopatología , Vasoconstricción , Vasoespasmo Intracraneal/diagnóstico por imagen , Vasoespasmo Intracraneal/fisiopatología
18.
Artículo en Inglés | MEDLINE | ID: mdl-34886574

RESUMEN

The impact of chemical to organic fertilizer substitution on soil labile organic and stabilized N pools under intensive farming systems is unclear. Therefore, we analyzed the distribution of soil total N (STN), particulate organic N (PON), microbial biomass N (MBN), dissolved organic N (DON), and mineral N (NO3- and NH4+) levels down to 100 cm profile under wheat-maize rotation system in northern China. The experiment was established with four 270 kg ha-1 N equivalent fertilizer treatments: Organic manure (OM); Organic manure with nitrogen fertilizer (OM + NF); Nitrogen fertilizer (NF); and Control (CK). Results found that the OM and OM + NF treatments had significantly higher STN, PON, MBN, DON, and NO3- contents in 0-20 cm topsoil depths. Conversely, the NF treatment resulted in the highest (p < 0.01) DON and NO3- depositions in 40-100 cm subsoil depths. The NH4+ contents in selected profile depths were significantly highest (p < 0.01) under OM treatment. The correlations between STN and its fractions were positively significant at 0-10 and 10-20 cm topsoil depths. Our results suggest that partial substitution of chemical fertilizer with organic manure could be a sustainable option for soil N management of intensive farming systems.


Asunto(s)
Fertilizantes , Suelo , Agricultura , Carbono/análisis , China , Fertilizantes/análisis , Estiércol , Nitrógeno/análisis
19.
Life Sci ; 231: 116563, 2019 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-31200003

RESUMEN

AIMS: In the present study, we investigated the roles of renin-angiotensin system (RAS) activation and imbalance of matrix metalloproteinase-9 (MMP-9)/tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in cold-induced stroke during chronic hypertension, as well as the protective effects of captopril and recombinant human TIMP-1 (rhTIMP-1). MAIN METHODS: Rats were randomly assigned to sham; 2-kidney, 2-clip (2K-2C); 2K-2C + captopril, and 2K-2C + rhTIMP-1 groups. After blood pressure values had stabilized, each group was randomly divided into an acute cold exposure (ACE) group (12-h light at 22 °C/12-h dark at 4 °C) and a non-acute cold exposure (NACE) group (12-h light/12-h dark at 22 °C), each of which underwent three cycles of exposure. Captopril treatment was administered via gavage (50 mg/kg/d), while rhTIMP-1 treatment was administered via the tail vein (60 µg/kg/36 h). KEY FINDINGS: In the 2K-2C group, angiotensin II (AngII) and MMP-9 levels increased in both the plasma and cortex, while no such changes in TIMP-1 expression were observed. Cold exposure further upregulated AngII and MMP-9 levels and increased stroke incidence. Captopril and rhTIMP-1 treatment inhibited MMP-9 expression and activation and decreased stroke incidence in response to cold exposure. SIGNIFICANCE: The present study is the first to demonstrate that cold exposure exacerbates imbalance between MMP-9 and TIMP-1 by activating the RAS, which may be critical in the initiation of stroke during chronic hypertension. In addition, our results suggest that captopril and rhTIMP-1 exert protective effects against cold-induced stroke by ameliorating MMP-9/TIMP-1 imbalance.


Asunto(s)
Metaloproteinasa 9 de la Matriz/metabolismo , Sistema Renina-Angiotensina/fisiología , Accidente Cerebrovascular/metabolismo , Inhibidor Tisular de Metaloproteinasa-1/metabolismo , Angiotensina II/metabolismo , Animales , Presión Sanguínea/efectos de los fármacos , Captopril/metabolismo , Captopril/farmacología , Proteínas de Ciclo Celular/metabolismo , Frío/efectos adversos , Humanos , Riñón/metabolismo , Masculino , Metaloproteinasa 1 de la Matriz/metabolismo , Metaloproteinasa 2 de la Matriz/metabolismo , Ratas , Ratas Sprague-Dawley , Proteínas Recombinantes/farmacología , Sistema Renina-Angiotensina/genética , Accidente Cerebrovascular/fisiopatología , Inhibidor Tisular de Metaloproteinasa-1/farmacología , Inhibidor Tisular de Metaloproteinasa-2
20.
Sci Rep ; 7(1): 213, 2017 03 16.
Artículo en Inglés | MEDLINE | ID: mdl-28303027

RESUMEN

Climate change and the growing demand for food security force growers to identify ways both to improve food production and to reduce agricultural carbon emissions. Although straw mulching is known to decrease CO2 emissions, winter wheat grain yield in the North China Plain was declined under straw mulching. In an effort to determine the most effective way to increase winter wheat yield under straw mulching, a field experiment was conducted using two planting patterns (wide-precision planting and conventional-cultivation planting) and two straw mulching rates (0 and 0.6 kg/m2). The results showed the wide-precision planting/non-mulching treatment significantly increased the leaf area index more than the other three treatments at the early growth stage. This treatment improved aboveground dry matter accumulation and was conducive to increased spike weight in the late growth stage. By contrast, straw mulching significantly reduced winter wheat grain yields by lowering both spike number and 1000-grain weight at the mature plant stage. In the wide-precision planting/mulching treatment, a significantly increased spike number compensated for grain yield losses. The results support the idea that wide-precision planting combined with straw mulching has the potential to decrease the winter wheat grain yield reduction previously observed with straw mulching in the North China Plain.

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