RESUMEN
OBJECTIVE: To clone the defensin genes from the Chinese main mosquito vectors and conduct sequnce analysis. METHODS: The genomic DNA and total RNA were extracted from Aedes aegypti, Aedes albopictus, Culex fatigans and Anopheles sinensis respectively, followed by PCR and reverse transcriptase-PCR with 2 pairs of primers designed and synthesized on the basis of reported defensin gene sequence. Purification and sequence analysis of the PCR products derived from the above procedure were conducted. RESULTS: The products with predicted size were amplified from the genomic DNA of A.aegypti, A.albopictus and A.sinensis. Sequence analysis showed that the amplified fragments from A.aegypti and A.albopictus, but not that from A.sinensis, were highly homologous with the reported defensin sequence. CONCLUSION: The defensin gene of A.aegypti and A.albopictus have been successfully cloned, and the homology of defensin genes among different mosquitoes is parallel to their genetic distances.
Asunto(s)
Aedes/genética , Anopheles/genética , Culex/genética , Defensinas/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , ADN Complementario , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa/métodos , Análisis de Secuencia de ADN , Homología de Secuencia de Ácido NucleicoRESUMEN
OBJECTIVE: To analyze the current situation on China Infectious Disease Automated-alert and Response System (CIDARS) in Guangdong province, China. METHODS: Early-warning signals and response time were analyzed by using three approaches of CIDARS. Positive rates of early-warning signals and error early-warning rates prior and after the adjustment,were compared. RESULTS: Totally, 114 585 early-warning signals appeared, with an average response time of 1.35 hours from April 21, 2008 to December 31, 2012. There were 12,394 early-warning signals in terms of fixed threshold method with a positive rate of 7.96%. 85 727 early-warning signals appeared under the mobile percentile method with a positive rate of 0.85%. There were 16,464 early-warning signals by using accumulation and control chart methods,with a positive rate of 1.82%. Results showed there was a positive correlation between the number of reported cases and the number of early-warning signals (r = 0.924, P < 0.01). The overall positive rate in Guangdong province increased from 1.48% to 2.14%, after the adjustment done by the Chinese Center for Disease Control and Prevention in 2010. The error early-warning rates regarding eight infectious diseases including hepatitis A, bacillary or amebic dysentery, both typhoid and paratyphoid fevers had reduced. CONCLUSION: The early-warning signal response appeared timely in CIDARS with good operation. However, despite the improvement on the efficacy of CIDARS, some functions and parameters of the systems still need to be adjusted.