RESUMEN
This work describes the characterization and genome annotation of Salmonella phage vB_SalM_8-19 (referred to as 8-19) isolated from sewage samples collected in a pig farm in Jilin, China. This phage was capable of infecting 60% Salmonella strains in our lab stock. The genome of phage 8-19 is composed of linear double-stranded DNA that is 52,648 bp in length with a G + C content of 46.02%; containing 74 ORFs and no tRNA genes. In October 2019, phylogenetic analyses indicated that phage 8-19 might belong to a novel cluster among the other similar phages which have not been specifically classified within some new genus in family Myoviridae. Recently, the International Committee on Taxonomy of Viruses (ICTV) defined phage 8-19 and its related phages as genus Rosemountvirus, family Myoviridae. This new genus, known as Rosemountvirus, is rarely reported in the literature.
Asunto(s)
Genoma Viral , Myoviridae/genética , Fagos de Salmonella/genética , Animales , Composición de Base , China , Myoviridae/clasificación , Myoviridae/aislamiento & purificación , Sistemas de Lectura Abierta , Filogenia , Fagos de Salmonella/clasificación , Fagos de Salmonella/aislamiento & purificación , Aguas del Alcantarillado/virología , PorcinosRESUMEN
This paper proposes an artifact-suppressed stimulator with a referenced and tuned push-pull stimulation (RTPPS) scheme where a tri-polar electrode is employed. The stimulation pulses delivered to two working electrodes are complementary and thus one counteracts with the other to suppress the artifact at the recording site. The prototype stimulator chip is implemented in 0.18-µm CMOS process technology. In-vivo experiments are carried out with the chip to demonstrate the proposed artifact-suppression technique in a simultaneous neural recording and stimulation system. The results show that the stimulation artifact can be greatly reduced. The amplitude of the measured stimulation artifact is therefore constrained in a negligible level (10%-20%) compared to recorded neural spikes.