Asunto(s)
Ataxia Cerebelosa/genética , Pancitopenia/genética , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética , Proteínas Supresoras de Tumor/genética , Adolescente , Adulto , Ataxia Cerebelosa/complicaciones , Ataxia Cerebelosa/patología , Preescolar , Aberraciones Cromosómicas , Femenino , Mutación de Línea Germinal , Humanos , Lactante , Masculino , Pancitopenia/complicaciones , Pancitopenia/patología , Linaje , Leucemia-Linfoma Linfoblástico de Células Precursoras/complicaciones , Leucemia-Linfoma Linfoblástico de Células Precursoras/patología , Pronóstico , SíndromeAsunto(s)
Anemia de Diamond-Blackfan/genética , Mutación , Niño , Humanos , Masculino , Linaje , Fenotipo , Proteínas Ribosómicas/genéticaRESUMEN
This study describe the use of a combination of two recently proposed typing approaches, multiple amplification of prophage locus typing (MAPLT) and multiple-locus variable-number tandem-repeat analysis (MLVA) for subdividing within Salmonella enterica serovar Heidelberg (S. Heidelberg). The combined typing method was compared with pulsed-field gel electrophoresis (PFGE) by Simpson's index of diversity (DI). PFGE was shown to have a DI = 0.84 and was poor at differentiation of the predominant PT1 (Phage Type 1) phenotype. In comparison, the combined MAPLT/MLVA method comprising 3 MLVA and 9 MAPLT primer pairs provided a higher differentiating ability DI = 0.92. More importantly, the combined methodology was found to be superior in the differentiation of the predominant PT1 isolates. In conclusion, this study demonstrated the potential of the rapid and simple amalgamated MAPLT/MLVA approach in determining transmission of isolates of clonal phage type groups from various environmental sources to humans.