RESUMEN
BACKGROUND: Clinical data are limited in patients with vitiligo with or without autoimmune thyroid disease. OBJECTIVES: The objective of the study was to investigate the clinical features and basic data of patients with vitiligo, especially those with autoimmune thyroid disease. METHODS: The study was a single-center retrospective study. A total of 1305 patients with vitiligo from June 2018 to May 2023 were included, and the clinical characteristics and basic information of the patients were recorded in detail. RESULTS: We identified an association between sex (odds ratio [OR]: 2.380; 95% confidence interval [CI]: 1.772-1.198), vitiligo duration (OR: 1.449; 95% CI: 1.076-1.952), skin involvement exceeding 5% of the body surface area (OR: 3.764; 95% CI: 2.134-6.640), negative emotions (OR: 3.076; 95% CI: 2.292-4.127), vitiligo type (OR: 1.974; 95% CI: 1.096-3.555), family history of AITD (OR: 4.979; 95% CI: 2.687-9.225), and family history of AD (OR: 2.418; 95% CI: 1.410-4.146) and patients with vitiligo. CONCLUSIONS: For patients with statistically significant associated risk factors, differential diagnosis and early intervention should be performed.
Asunto(s)
Enfermedades Autoinmunes , Enfermedades de la Tiroides , Vitíligo , Humanos , Vitíligo/complicaciones , Vitíligo/epidemiología , Vitíligo/diagnóstico , Estudios Retrospectivos , Factores de Riesgo , Superficie Corporal , Enfermedades de la Tiroides/complicaciones , Enfermedades de la Tiroides/epidemiología , Enfermedades Autoinmunes/complicaciones , Enfermedades Autoinmunes/epidemiologíaRESUMEN
Cloning the segment of polyhedrin gene which was separated from AcMNPV genome DNA by PCR method into transfer vector pBacPAK8, we got recombinant transfer vector pOAc, then contransfecting BmN cells with the vector and linear virus Bm-BacPAK6, harvested recombinant virus hp-BmNPV which can produce polyhedrin but no blue plaques. Analysing of recombinant virus from polyhedrin, recombinant viral DNA and polyhedra, we confirmed that polyhedring of AcMNPV can be not only high-level expressed in BmN cells, But can recognize recombinant BmNPV DNA and assemble them to polyhedra. We observed that the pattern of restriction enzyme changed due to DNA recombination, the size of recombinant viral polyhedron (1.2-2.9 microns) was obviously smaller than those of wild-type BmNPV by electron microscopy.
Asunto(s)
Bombyx/virología , Genes Virales , Lepidópteros/virología , Nucleopoliedrovirus/genética , Proteínas Virales/genética , Animales , Bombyx/metabolismo , Clonación Molecular , ADN Viral/genética , Nucleopoliedrovirus/fisiología , Proteínas de la Matriz de Cuerpos de Oclusión , Regiones Promotoras Genéticas , Transfección , Proteínas Virales/biosíntesis , Proteínas Estructurales Virales , Ensamble de VirusRESUMEN
According to the reported sequence of Buthus martensii Karsch scorpion toxin gene (BmK IT3), we synthesized two primers, which were complementary in a region. By the means of PCR, we got the gene. The gene was fused in expression vector pET-28a, which gave rise to a recombinant plasmid pET(IT3R). Then it was transformed into E. coli BL21 (DE3). With IPTG induction, the gene was efficiently expressed. And the fusion product was soluble.