RESUMEN
The authors measured the diameters of vessels around the surgical plane of tonsillectomy to investigate an anatomical basis to reduce hemorrhage. Thirty tonsils removed from 15 adult cadavers with the mean age of 56 years (range 44-71 years) at the time of death were studied. Calibration of the vessels across the tonsillar capsule was performed at the 1-mm intracapsular, capsular, and 1-mm extracapsular plane as artery and vein, respectively. The average diameter of the arteries was 73.0 +/- 33.1 mum at the 1-mm intracapsular plane, 94.7 +/- 33.5 mum at the capsular plane, and 139.5 +/- 51.2 mum at the 1-mm extracapsular plane. For the veins, it was 62.9 +/- 38.7 mum at the 1-mm intracapsular plane, 86.8 +/- 50.4 mum at the capsular plane, and 133.6 +/- 78.6 mum at the 1-mm extracapsular plane. The diameters of the vessels at the 1-mm intracapsular plane were significantly smaller than those at the capsular plane (P < 0.01), and likewise the diameters of the vessels at the capsular plane were significantly smaller than those at the 1-mm extracapsular plane (P < 0.01). The result of this study on the diameter of the vessels across the tonsillar capsule could be considered to be an important factor providing an anatomical rationale for a change in recommendation leading to safer tonsillectomies that minimize vascular injury.
Asunto(s)
Arterias/anatomía & histología , Tonsila Palatina/irrigación sanguínea , Tonsilectomía/métodos , Venas/anatomía & histología , Adulto , Anciano , Femenino , Humanos , Complicaciones Intraoperatorias/prevención & control , Masculino , Persona de Mediana Edad , Hemorragia Posoperatoria/prevención & control , Tonsilectomía/efectos adversosRESUMEN
BACKGROUND: The applicability of the melanoma antigen gene (MAGE) to the molecular diagnosis of cancer, by detecting MAGE mRNA in the sputa of head and neck cancer patients, was investigated. MATERIALS AND METHODS: Nested reverse transcription-polymerase chain reaction (RT-PCR) with the MAGE common primers designed to detect MAGE A1-6 genes was conducted with sputa of 17 head and neck squamous cell carcinoma patients. RESULTS: Of the 17 sputa specimens from patients with squamous cell carcinoma, MAGE mRNA was detected in 13 cases, while the detection rate was 2 out of 40 in the normal control group, showing significant correlation (p<0.05) between head and neck squamous cell carcinoma and MAGE mRNA expression in sputa. CONCLUSION: MAGE may be a useful tumor marker of head and neck cancer.
Asunto(s)
Carcinoma de Células Escamosas/metabolismo , Neoplasias de Cabeza y Cuello/metabolismo , Proteínas de Neoplasias/biosíntesis , ARN Mensajero/biosíntesis , Adulto , Antígenos de Neoplasias , Biomarcadores de Tumor/biosíntesis , Biomarcadores de Tumor/genética , Carcinoma de Células Escamosas/genética , Femenino , Estudios de Seguimiento , Neoplasias de Cabeza y Cuello/genética , Humanos , Masculino , Antígenos Específicos del Melanoma , Persona de Mediana Edad , Proteínas de Neoplasias/genética , ARN Mensajero/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Esputo/química , Esputo/metabolismoRESUMEN
Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these represent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.