Serpin family E member 1 enhances myometrium contractility by increasing ATP production during labor.

The uterine contraction during labor, a process with repetitive hypoxia and high energy consumption, is essential for successful delivery. However, the molecular mechanism of myometrial contraction regulation is unknown. Serpin family E member 1 (SERPINE1), one of the most upregulated genes in laboring myometrium in both transcriptome and proteome, was highlighted in our previous study. Here, we confirmed SERPINE1 is upregulated in myometrium during labor. Blockade of SERPINE1 using small interfering RNA (siRNA) or inhibitor (Tiplaxtinin) under hypoxic conditions in myocytes or myometrium in vitro showed a decrease contractility, which was achieved by regulating ATP production. Chromatin immunoprecipitation (ChIP-seq), Co-immunoprecipitation (Co-IP), and glutathione-S-transferase (GST) pull down explored that the promoter of SERPINE1 is directly activated by hypoxia-inducible factor-1α (HIF-1α) and SERPINE1 interacts with ATP Synthase Peripheral Stalk Subunit F6 (ATP5PF). Together they enhance hypoxia driven myometrial contraction by maintaining ATP production in the key oxidative phosphorylation pathway. The results provide new insight for uterine contraction regulation, and potential novel therapeutic targets for labor management.

The LARGE2-APO1/APO2 regulatory module controls panicle size and grain number in rice.

Panicle size and grain number are important agronomic traits and influence grain yield in rice (Oryza sativa), but the molecular and genetic mechanisms underlying panicle size and grain number control remain largely unknown in crops. Here we report that LARGE2 encodes a HECT-domain E3 ubiquitin ligase OsUPL2 and regulates panicle size and grain number in rice. The loss of function large2 mutants produce large panicles with increased grain number, wide grains and leaves, and thick culms. LARGE2 regulates panicle size and grain number by repressing meristematic activity. LARGE2 is highly expressed in young panicles and grains. Biochemical analyses show that LARGE2 physically associates with ABERRANT PANICLE ORGANIZATION1 (APO1) and APO2, two positive regulators of panicle size and grain number, and modulates their stabilities. Genetic analyses support that LARGE2 functions with APO1 and APO2 in a common pathway to regulate panicle size and grain number. These findings reveal a novel genetic and molecular mechanism of the LARGE2-APO1/APO2 module-mediated control of panicle size and grain number in rice, suggesting that this module is a promising target for improving panicle size and grain number in crops.

Microfluidics-enabled fluorinated assembly of EGCG-ligands-siTOX nanoparticles for synergetic tumor cells and exhausted t cells regulation in cancer immunotherapy.

Immune checkpoint inhibitor (ICI)-derived evolution offers a versatile means of developing novel immunotherapies that targets programmed death-ligand 1 (PD-L1)/programmed death-1 (PD-1) axis. However, one major challenge is T cell exhaustion, which contributes to low response rates in "cold" tumors. Herein, we introduce a fluorinated assembly system of LFNPs/siTOX complexes consisting of fluorinated EGCG (FEGCG), fluorinated aminolauric acid (LA), and fluorinated polyethylene glycol (PEG) to efficiently deliver small interfering RNA anti-TOX (thymus high mobility group box protein, TOX) for synergistic tumor cells and exhausted T cells regulation. Using a microfluidic approach, a library of LFNPs/siTOX complexes were prepared by altering the placement of the hydrophobe (LA), the surface PEGylation density, and the siTOX ratio. Among the different formulations tested, the lead formulation, LFNPs3-3/siTOX complexes, demonstrated enhanced siRNA complexation, sensitive drug release, improved stability and delivery efficacy, and acceptable biosafety. Upon administration by the intravenous injection, this formulation was able to evoke a robust immune response by inhibiting PD-L1 expression and mitigating T cell exhaustion. Overall, this study provides valuable insights into the fluorinated assembly and concomitant optimization of the EGCG-based delivery system. Furthermore, it offers a promising strategy for cancer immunotherapy, highlighting its potential in improving response rates in ''cold'' tumors.

Transcriptomic analysis at the first instar larval stage of nonmolting Bombyx mori mutant (a42).

The life cycle of the holometabolous insect Bombyx mori (Linnaeus) consists of the embryo, larva, pupa, and adult stages with six larval molts. Ecdysone and juvenile hormones play important roles in the growth and development of the silkworms. The a42 silkworm mutant is recessive and homozygous lethal by exhibiting a dark-colored and small body size and fails to molt to second instar. We compared the gene expression of a42 mutants with normal individuals at the first larval molting stage to elucidate the physiological influence of the a42 mutation on the growth and development of silkworms. The transcriptomic sequencing results revealed that 1,411 genes are differentially expressed in a42 mutants, compared with wild-type control silkworms, in which 791 genes are upregulated and 620 genes are downregulated. Gene Ontology/Kyoto Encyclopedia of Genes and Genomes analyses identified differentially expressed genes (DEGs) assigned to biological pathways, such as pentose and glucoronate interconversions, glycerolipid metabolism, folate biosynthesis, amino sugar, and nucleotide sugar metabolism. Two hydroxylases of phenylalanine hydroxylase (BmPAH) and tyrosine hydroxylase (BmTh) are upregulated in a42 mutants. The influence of a42 mutation on these DEGs reveals that melanin metabolism plays an important role during the molting process in silkworms.

Indomethacin down-regulating HMGB1 and TNF-α to prevent pancreatitis after endoscopic retrograde cholangiopancreatography.

Background and aims: Several articles demonstrated that non-steroidal anti-inflammation drugs (NSAIDs) were effective in reducing the incidence of pancreatitis after endoscopic retrograde cholangiopancreatography (PEP). However, studies revealed inconsistent results. The mechanism of NSAIDs in preventing PEP is still little known. Therefore, the aim of our study was to evaluate the efficacy of NSAIDs for PEP prophylaxis and further to explore the mechanism of NSAIDs for prevention of PEP. Methods: Patients who underwent endoscopic retrograde cholangiopancreatography (ERCP) were randomly assigned to receive 100 mg rectal indomethacin or glycerin suppository 15-20 min before ERCP. The primary outcome was the rate of PEP. And the levels of serum HMGB1 and TNF-α were also measured before ERCP and 3 and 24 h after ERCP. Univariate analysis and multivariate analysis were carried out to estimate the independent risk factors for PEP. Results: Totally, 100 patients were enrolled, 50 received indomethacin and 50 with placebo (glycerin suppository). PEP developed in six patients in indomethacin group and 16 in the control group, the difference was significant (p = .016). The levels of HMGB1 and TNF-α were significantly decreased in indomethacin group at 3 (p < .0001) and 24 h (p < .0001) after ERCP, compared to the control group. Multivariate analysis revealed that duration of ERCP (OR, 0.221; 95% CI, 0.072-0.680; p = .008) and usage of NSAIDs (OR, 0.278; 95% CI, 0.090-0.861; p = .026) were independent predictors of PEP. Conclusions: Rectal indomethacin could significantly reduce the risk of PEP by down-regulating the levels of HMGB1 and TNF-α.

Expression Analysis of mRNA Decay of Maternal Genes during Bombyx mori Maternal-to-Zygotic Transition.

Maternal genes play an important role in the early embryonic development of the silkworm. Early embryonic development without new transcription depends on maternal components stored in the egg during oocyte maturation. The maternal-to-zygotic transition (MZT) is a tightly regulated process that includes maternal mRNAs elimination and zygotic transcription initiation. This process has been extensively studied within model species. Each model organism has a unique pattern of maternal transcriptional clearance classes in MZT. In this study, we identified 66 maternal genes through bioinformatics analysis and expression analysis in the eggs of silkworm virgin moths (Bombyx mori). All 66 maternal genes were expressed in vitellogenesis in day eight female pupae. During MZT, the degradation of maternal gene mRNAs could be divided into three clusters. We found that eight maternal genes of cluster 1 remained stable from 0 to 3.0 h, 17 maternal genes of cluster 2 were significantly decayed from 0.5 to 1.0 h and 41 maternal genes of cluster 3 were significantly decayed after 1.5 h. Therefore, the initial time-point of degradation of cluster 2 was earlier than that of cluster 3. The maternal gene mRNAs decay of clusters 2 and 3 is first initiated by maternal degradation activity. Our study expands upon the identification of silkworm maternal genes and provides a perspective for further research of the embryo development in Bombyx mori.

Study on the Role of Cytc in Response to BmNPV Infection in Silkworm, Bombyx mori (Lepidoptera).

Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the primary pathogens of the silkworm. Cytochrome c (cytc) showed a significant response to BmNPV infection in our previous transcriptome study. However, little is known about the role of Bombyx mori cytc (Bmcytc) in resistance to BmNPV infection. In this study, the expression levels analysis of Bmcytc showed stable expression levels in selected tissues of the resistant strain AN following BmNPV infection, while there was downregulation in the susceptible strain p50, except in the malpighian tubule. To further study the role of Bmcytc in viral infection, Bmcytc was knocked down with siRNA in vitro, resulting in significant downregulation of selected downstream genes of the mitochondrial pathway, including Bmapaf, Bmcaspase-Nc, and Bmcaspase-1; this was also confirmed by overexpression of Bmcytc using the pIZT/V5-His-mCherry insect vector, except Bmcaspase-1. Moreover, knockdown of Bmcytc significantly promoted the infection process of BmNPV in vitro, while the infection was inhibited by overexpression of Bmcytc at the early stage and subsequently increased rapidly. Based on these results, we concluded that Bmcytc plays a vital role in BmNPV infection by regulating the mitochondrial apoptosis pathway. Our work provides valuable data for the clarification of the mechanism of silkworm resistance to BmNPV infection.

Quantification of Water, Protein and Soluble Sugar in Mulberry Leaves Using a Handheld Near-Infrared Spectrometer and Multivariate Analysis.

Mulberry (Morus alba L.) leaves are not only used as the main feed for silkworms (Bombyx mori) but also as an added feed for livestock and poultry. In order to rapidly select high-quality mulberry leaves, a hand-held near-infrared (NIR) spectrometer combined with partial least squares (PLS) regression and wavelength optimization methods were used to establish a predictive model for the quantitative determination of water content in fresh mulberry leaves, as well as crude protein and soluble sugar in dried mulberry leaves. For the water content in fresh mulberry leaves, the R-square of the calibration set (R2 C), R-square of the cross-validation set (R2 CV) and R-square of the prediction set (R2 P) are 0.93, 0.90 and 0.91, respectively, the corresponding root mean square error of calibration set (RMSEC), root mean square error of cross-validation set (RMSECV) and root mean square error of prediction set (RMSEP) are 0.96%, 1.13%, and 1.18%, respectively. The R2 C, R2 CV and R2 P of the crude protein prediction model are 0.91, 0.83 and 0.92, respectively, and the corresponding RMSEC, RMSECV and RMSEP are 0.71%, 0.97% and 0.61%, respectively. The soluble sugar prediction model has R2 C, R2 CV, and R2 P of 0.64, 0.51, and 0.71, respectively, and the corresponding RMSEC, RMSECV, and RMSEP are 2.33%, 2.73%, and 2.36%, respectively. Therefore, the use of handheld NIR spectrometers combined with wavelength optimization can fastly detect the water content in fresh mulberry leaves and crude protein in dried mulberry leaves. However, it is a slightly lower predictive performance for soluble sugar in mulberry leaves.

Quantitative Analysis of Organic Liquid Three-Component Systems: Near-Infrared Transmission versus Raman Spectroscopy, Partial Least Squares versus Classical Least Squares Regression Evaluation and Volume versus Weight Percent Concentration Units.

The band shapes and band positions of near-infrared (NIR) and Raman spectra change depending on the concentrations of specific chemical functionalities in a multicomponent system. To elucidate these effects in more detail and clarify their impact on the analytical measurement techniques and evaluation procedures, NIR transmission spectra and Raman spectra of two organic liquid three-component systems with variable compositions were analyzed by two different multivariate calibration procedures, partial least squares (PLS) and classical least-squares (CLS) regression. Furthermore, the effect of applying different concentration units (volume percent (%V) and weight percent (%W) on the performance of the two calibration procedures have been tested. While the mixtures of benzene/cyclohexane/ethylbenzene (system 1) can be regarded as a blended system with comparatively low molecular interactions, hydrogen bonding plays a dominant role in the blends of ethyl acetate/1-heptanol/1,4-dioxane (system 2). Whereas system 1 yielded equally good calibrations by PLS and CLS regression, for system 2 acceptable results were only obtained by PLS regression. Additionally, for both sample systems, Raman spectra generally led to lower calibration performance than NIR spectra. Finally, volume and weight percent concentration units yielded comparable results for both chemometric evaluation procedures.

EZH2 promotes cell proliferation by regulating the expression of RUNX3 in laryngeal carcinoma.

Enhancer of zeste homolog 2 (EZH2) is a highly conserved histone methyltransferase, which is overexpressed in different types of cancers such as breast and prostate cancer. It is reported that EZH2 can directly down-regulate RUNX3 by increasing histone H3 methylation. However, the role of EZH2 in the development and progression of laryngeal carcinoma has not yet been investigated, and the relationship between EZH2 and RUNX3 in laryngeal carcinoma is rarely reported. The current study aims to determine the role of EZH2 in the progression of laryngeal carcinoma, and investigate the interaction between EZH2 and the tumor suppressor RUNX3. Our study found that EZH2 is overexpressed in laryngeal carcinoma patients, and silencing EZH2 by EZH2 siRNA significantly inhibited the proliferation of laryngeal carcinoma cells. Besides, we also found that RUNX3 is repressed in laryngeal carcinoma patients. Moreover, RUNX3 as a downstream target protein of EZH2 is up-regulated by EZH2 siRNA accompanied by a decrease in the trimethylation modification pattern of H3K27. RUNX3 siRNA inhibits the decreased proliferation induced by EZH2 siRNA. Furthermore, ß-catenin protein expression is down-regulated by EZH2 siRNA and up-regulated by RUNX3 siRNA, and RUNX3 siRNA inhibits the down-regulation effect of EZH2 siRNA on ß-catenin protein expression. Additionally, the Wnt/ß-catenin activator BIO reverses the inhibitory effect of EZH2 siRNA on Hep-2 cell proliferation. Taken together, our results suggest that EZH2 regulates cell proliferation potentially by targeting RUNX3 through the Wnt/ß-catenin signaling pathway in laryngeal carcinoma.

20-Hydroxyecdysone (20E) Primary Response Gene E93 Modulates 20E Signaling to Promote Bombyx Larval-Pupal Metamorphosis.

As revealed in a previous microarray study to identify genes regulated by 20-hydroxyecdysone (20E) and juvenile hormone (JH) in the silkworm, Bombyx mori, E93 expression in the fat body was markedly low prior to the wandering stage but abundant during larval-pupal metamorphosis. Induced by 20E and suppressed by JH, E93 expression follows this developmental profile in multiple silkworm alleles. The reduction of E93 expression by RNAi disrupted 20E signaling and the 20E-induced autophagy, caspase activity, and cell dissociation in the fat body. Reducing E93 expression also decreased the expression of the 20E-induced pupal-specific cuticle protein genes and prevented growth and differentiation of the wing discs. Importantly, the two HTH domains in E93 are critical for inducing the expression of a subset of 20E response genes, including EcR, USP, E74, Br-C, and Atg1. By contrast, the LLQHLL and PLDLSAK motifs in E93 inhibit its transcriptional activity. E93 binds to the EcR-USP complex via a physical association with USP through its LLQHLL motif; and this association is enhanced by 20E-induced EcR-USP interaction, which attenuates the transcriptional activity of E93. E93 acts through the two HTH domains to bind to GAGA-containing motifs present in the Atg1 promoter region for inducing gene expression. In conclusion, E93 transcriptionally modulates 20E signaling to promote Bombyx larval-pupal metamorphosis.

Association analysis for detecting significant single nucleotide polymorphisms for phosphorus-deficiency tolerance at the seedling stage in soybean [Glycine max (L) Merr].

Tolerance to low-phosphorus soil is a desirable trait in soybean cultivars. Previous quantitative trait locus (QTL) studies for phosphorus-deficiency tolerance were mainly derived from bi-parental segregating populations and few reports from natural population. The objective of this study was to detect QTLs that regulate phosphorus-deficiency tolerance in soybean using association mapping approach. Phosphorus-deficiency tolerance was evaluated according to five traits (plant shoot height, shoot dry weight, phosphorus concentration, phosphorus acquisition efficiency and use efficiency) comprising a conditional phenotype at the seedling stage. Association mapping of the conditional phenotype detected 19 SNPs including 13 SNPs that were significantly associated with the five traits across two years. A novel cluster of SNPs, including three SNPs that consistently showed significant effects over two years, that associated with more than one trait was detected on chromosome 3. All favorable alleles, which were determined based on the mean of conditional phenotypic values of each trait over the two years, could be pyramided into one cultivar through parental cross combination. The best three cross combinations were predicted with the aim of simultaneously improving phosphorus acquisition efficiency and use efficiency. These results will provide a thorough understanding of the genetic basis of phosphorus deficiency tolerance in soybean.

Detection and fine-mapping of SC7 resistance genes via linkage and association analysis in soybean.

Soybean mosaic virus (SMV) disease is one of the most serious and broadly distributed soybean (Glycine max (L.) Merr.) diseases. Here, we combine the advantages of association and linkage analysis to identify and fine-map the soybean genes associated with resistance to SMV strain SC7. A set of 191 soybean accessions from different geographic origins and 184 recombinant inbred lines (RILs) derived from Kefeng No.1 (resistant) × Nannong 1138-2 (susceptible) were used in this study. The SC7 resistance genes were previously mapped to a 2.65 Mb region on chromosome 2 and a 380 kb region on chromosome 13. Among 19 single nucleotide polymorphisms (SNPs) detected via association analysis in the study, the SNP BARC-021625-04157 was located in the 2.65 Mb region, and the SNP BARC-041671-08065 was located near the 380 kb region; three genes harboring the SNPs were probably related to SC7 resistance. The resistance gene associated with BARC-021625-04157 was then fine-mapped to a region of approximately 158 kb on chromosome 2 using 184 RILs. Among the 15 genes within this region, one NBS-LRR type gene, one HSP40 gene and one serine carboxypeptidase-type gene might be candidate SC7 resistance genes. These results will be useful for map-based cloning and marker-assisted selection in soybean breeding programs.

Activation of BNGR-A24 by direct interaction with tachykinin-related peptides from the silkworm Bombyx mori leads to the Gq- and Gs-coupled signaling cascades.

Tachykinins constitute one of the largest peptide families in the animal kingdom and exert their diverse actions via G protein-coupled receptors (GPCRs). In this study, the Bombyx tachykinin-related peptides (TKRPs) were identified as specific endogenous ligands for the Bombyx neuropeptide GPCR A24 (BNGR-A24) and thus designated BNGR-A24 as BmTKRPR. Using both mammalian cell line HEK293 and insect cell line Sf21, further characterization demonstrated that BmTKRPR was activated, thus resulting in intracellular accumulation of cAMP, Ca(2+) mobilization, and ERK1/2 phosphorylation in a Gs and Gq inhibitor-sensitive manner. Moreover, quantitative reverse transcriptase polymerase chain reaction analysis and dsRNA-mediated knockdown experiments suggested a possible role for BmTKRPR in the regulation of feeding and growth. Our findings enhance the understanding of the Bombyx TKRP system in the regulation of fundamental physiological processes.

Identification and functional analysis of the cathepsin D gene promoter of Bombyx mori.

The gene encoding cathepsin D of silkworm, Bombyx mori (BmCatD) is specifically expressed in the larval fat body and pupal gut, and plays an important role in the programmed cell death during metamorphosis. To identify element involved in this transcription-dependent spatial restriction, truncation and deletion of the 5' terminal from the BmCatD promoter were conducted in vivo. The recombinant AcMNPV vector (Autographa californica multiple nucleopolyhedrovirus) with a dual-luciferase quantitative assay system was used as the transfer. A 289 bp DNA sequence (-1,214 to -925) upstream of the transcriptional start site is found to be responsible for promoting tissue-specific transcription. Further analysis of a series of deletion within the 289 bp region of overlapping deletion showed that a 33 bp region (-1,071 to -1,038) sequence suppresses the ectopic expression of the BmCatD promoter. These results suggest that this 33 bp region could function as a promoter element in the tissue-specificity expression.

Suppression of T-type Ca2+ channels inhibited human laryngeal squamous cell carcinoma cell proliferation running title: roles of T-type Ca2+ channels in LSCC cell proliferation.

BACKGROUND: Despite the tight correlation between T-type Ca2+ channels and a great variety of tumors, the roles of alpha1G subunit of T-type Ca2+ channels in laryngeal squamous cell carcinoma (LSCC) have not yet been investigated. METHODS: In the present study, we examined the expression of alpha1G subunit of T-type Ca2+ channel in human LSCC tissues and cell lines. One human laryngeal squamous cell carcinoma cell line, Hep-2, was also examined for T-type channels using voltage-clamp recordings. Cell proliferation assays were performed in the presence or absence of T-type channel blocker mibefradil and alpha1G subunit sepcific siRNA. The cell cycle was determined by flow cytometry. RESULTS: Our results indicated that the a1G subunit of T-type Ca2+ channel is highly expressed in human laryngeal squamous cell carcinoma tissues and cell lines. alpha1G siRNA significantly down-regulated the protein expression of the alpha1G subunit. Both alpha1G siRNA and mibefradil inhibited Hep-2 cell proliferation and arrested cell cycle progression. CONCLUSIONS: Together, these findings suggest a functional role of T-type channels in certain laryngeal carcinomas, and that inhibition of T-type channels reduces cell proliferation via cell cycle arrest, suggesting that the alpha1G subunit of T-type Ca2+ channel may be used as a therapeutic target for treating LSCC.

Association mapping of yield-related traits and SSR markers in wild soybean (Glycine soja Sieb. and Zucc.).

Wild soybean, the progenitor of cultivated soybean, is an important gene pool for ongoing soybean breeding efforts. To identify yield-enhancing quantitative trait locus (QTL) or gene from wild soybean, 113 wild soybeans accessions were phenotyped for five yield-related traits and genotyped with 85 simple sequence repeat (SSR) markers to conduct association mapping. A total of 892 alleles were detected for the 85 SSR markers, with an average 10.49 alleles; the corresponding PIC values ranged from 0.07 to 0.92, with an average 0.73. The genetic diversity of each SSR marker ranged from 0.07 to 0.93, with an average 0.75. A total of 18 SSR markers were identified for the five traits. Two SSR markers, sct_010 and satt316, which are associated with the yield per plant were stably expressed over two years at two experimental locations. Our results suggested that association mapping can be an effective approach for identifying QTL from wild soybean.

Development and validation of a CT based radiomics nomogram for preoperative prediction of ISUP/WHO grading in renal clear cell carcinoma.

BACKGROUND: Nuclear grading of clear cell renal cell carcinoma (ccRCC) is crucial for its diagnosis and treatment. OBJECTIVE: To develop and validate a machine learning model for preoperative assessment of ccRCC nuclear grading using CT radiomics. MATERIALS AND METHODS: This retrospective study analyzed 146 ccRCC patients who underwent surgery between June 2016 and January 2022 at two hospitals (the Quzhou Affiliated Hospital of Wenzhou Medical University with 117 cases and the Affiliated Cancer Hospital of University of Chinese Academy of Sciences with 29 cases). Radiomic features were extracted from preoperative abdominal CT images. Features reduction and selection were carried out using intraclass correlation efficient (ICCs), Spearman rank correlation coefficientsand and the Least Absolute Shrinkage and Selection Operator (LASSO) regression method. Radiomics and clinical models were developed utilizing Support Vector Machine (SVM), Extremely Randomized Trees (Extra Trees), Light Gradient Boosting Machine (LightGBM), Random Forest (RF) and K-Nearest Neighbors (KNN) algorithms. Subsequently, the radiomics nomogramwas developed incorporating independent clinical predictors and Rad_signature. Model performance was evaluated using the area under the curve (AUC), accuracy, sensitivity, and specificity, with decision curve analysis (DCA) assessing its clinical utility. RESULTS: We extracted 1834 radiomic features from each CT sequence, with 1320 features passing through the ICCs screening process. 480 radiomics features were screened by Spearson correlation coefficient. Then, 15 radiomic features with non-zero coefficient values were determined by Lasso dimensionality reduction technique. The five machine learning methods effectively distinguished nuclear grades. The radiomics nomogram outperformed clinical radiological models and radiomics feature models in predictive performance, with an AUC of 0.936 (95% CI 0.885-0.986) for the training set and 0.896 (95% CI 0.716-1.000) for the external verification set. DCA indicated potential clinical applicability of the nomogram. CONCLUSION: The radiomics nomogram, developed by integrating clinically independent risk factors and and Rad_signature, demonstrated robust performance in preoperative ccRCC grading. It offers a non-invasive tool that aids in ccRCC grading and clinical decision-making, with potential to enhance treatment strategies.

Hot Deformation Characteristics and Dynamic Recrystallization Mechanisms of a Semi-Solid Forged AZ91D Magnesium Alloy.

Magnesium alloys show great promise in high-speed transport, aerospace, and military technology; however, their widespread adoption encounters challenges attributed to limitations such as poor plasticity and strength. This study examines the high-temperature deformation of semi-solid forged AZ91D magnesium alloy through a combination of experiments and simulations, with a focus on comprehending the influence of deformation conditions on dynamic recrystallization (DRX). The findings disclose that conspicuous signs of DRX manifest in the yield stress curve as strain increases. Additionally, decreasing the strain rate and temperature correlates with a reduction in both yield stress and peak strain, and the activation energy is 156.814 kJ/mol, while the critical strain and peak strain remain relatively consistent (εc=0.66208εp). Microstructural changes during high-temperature deformation and the onset of DRX are thoroughly examined through experimental methods. Moreover, a critical strain model for DRX and a predictive model for the volume fraction of DRX were formulated. These equations and models, validated through a combination of experiments and simulations, serve as invaluable tools for predicting the mechanical behavior and microstructural evolution, which also establishes a foundation for accurately predicting the deformation behavior of this alloy. By analyzing the hot deformation characteristics and dynamic compression mechanism of the newly developed semi-solid forging AZ91D magnesium alloy, a numerical simulation model can be effectively established. This model objectively reflects the changes and distributions of stress, strain, and rheological velocity, providing a scientific basis for selecting subsequent plastic deformation process parameters and designing mold structures.

Fast identification of the BmNPV infected silkworms by portable NIR spectroscopy and chemometrics.

A convenient, low-cost, and rapid detection of BmNPV-infected silkworms is of great significance for the safety of the sericulture industry. In this study, a portable NIR system was used to collect the spectra of normal silkworms and the infected silkworms induced by the administration of Bombyx mori nuclear polyhedrosis virus (BmNPV). Different spectral pretreatment methods were applied, then principal component analysis (PCA), linear discriminant analysis (LDA), and partial least squares discriminant analysis (PLSDA) were used for the classification analysis. The results showed that PCA and LDA were unable to achieve the purpose. For the PLSDA calibration, after the pretreatment of SNV combining 2nd derivative, it had a high identification performance, and obtained low classification errors of 0.023, 0.033, and 0.030 for the calibration set, cross-validation set, and test set, respectively, with higher sensitivity and specificity. Therefore, the BmNPV-infected silkworms can be identified by portable NIR spectroscopy, which will effectively reduce losses for the sericulture industry.