RESUMEN
Danshen Chuanxiongqin Injection is a commonly used medicine in cerebral infarction and coronary heart disease,which is recommended by many disease guidelines/consensus for cardiovascular and cerebrovascular diseases. However,there are irrational drug using in clinic,which affects the efficacy and brings safety risks. Based on clinical research evidence and expert experience,recommendations/consensus suggestion are determined through the nominal group method. The expert consensus recommends the indications,intervention time for treatment,route of drug administration,usage and dosage,course of treatment and introduces the safety in clinical application,which could provide reference for clinical use of Danshen Chuanxiongqin Injection.
Asunto(s)
Infarto Cerebral/tratamiento farmacológico , Medicamentos Herbarios Chinos/uso terapéutico , Consenso , Humanos , InyeccionesRESUMEN
OBJECTIVE: To observe the effect of Chinese herbal compound for supplementing qi and activating blood circulation (CHC) on the gap junctional intercellular communication (GJIC) function of myocardial cells in patients with Coxsackie virus B 3 (CVB3) viral myocarditis. METHODS: Expressions of actin and connexin43 (Cx43) in myocardial cells of patients arranged in three groups (the normal control group, the viral infected group and the CHC treated group) were detected by immunohistochemical method; the fluorescence photobleaching recovery rate of cells was detected by laser scanning confocal microscope. RESULTS: As compared with the viral infected group, the expressions of actin and Cx43 were increased and the GJIC function was improved in the CHC treated group. CONCLUSION: CHC could antagonize viral injury on skeleton protein, and repair the structure of gap junction channel to improve the GJIC function of myocardial cells after being attacked by CVB3.
Asunto(s)
Actinas/metabolismo , Conexina 43/metabolismo , Infecciones por Coxsackievirus/metabolismo , Medicamentos Herbarios Chinos/farmacología , Miocarditis/virología , Actinas/genética , Animales , Animales Recién Nacidos , Comunicación Celular/fisiología , Conexina 43/genética , Enterovirus Humano B , Uniones Comunicantes/fisiología , Miocarditis/metabolismo , Miocardio/metabolismo , Ratas , Ratas WistarRESUMEN
MicroRNA (miRNA) may function as an oncogene or a tumor suppressor in tumorigenesis. However, the mechanism of miRNAs in adenoid cystic carcinoma (ACC) is unclear. Here, we provide evidence that miR-24-3p was downreglated and functions as a tumor suppressor in human lacrimal adenoid cystic carcinoma by suppressing proliferation and migration/invasion while promoting apoptosis. miR-24-3p down-regulated protein kinase C eta (PRKCH) by binding to its untranslated region (3'UTR). PRKCH increased the of the cell growth and migration/invasion in ACC cells and suppressed the expression of p53 and p21 in both mRNA and protein level. The overexpression of miR-24-3p decreased its malignant phenotype. Ectopic expression of PRKCH counteracted the suppression of malignancy induced by miR-24-3p, as well as ectopic expression of miR-24-3p rescued the suppression of PRKCH in the p53/p21 pathway. These results suggest that miR-24-3p promotes the p53/p21 pathway by down-regulating PRKCH expression in lacrimal adenoid cystic carcinoma cells.
Asunto(s)
Carcinoma Adenoide Quístico/metabolismo , Neoplasias del Ojo/metabolismo , Enfermedades del Aparato Lagrimal/metabolismo , MicroARNs/genética , Proteína Quinasa C/genética , Regiones no Traducidas 3' , Carcinoma Adenoide Quístico/genética , Carcinoma Adenoide Quístico/patología , Línea Celular Tumoral , Inhibidor p21 de las Quinasas Dependientes de la Ciclina/genética , Inhibidor p21 de las Quinasas Dependientes de la Ciclina/metabolismo , Neoplasias del Ojo/genética , Neoplasias del Ojo/patología , Regulación Neoplásica de la Expresión Génica , Humanos , Enfermedades del Aparato Lagrimal/genética , Enfermedades del Aparato Lagrimal/patología , Proteína Quinasa C/metabolismo , Proteína p53 Supresora de Tumor/genética , Proteína p53 Supresora de Tumor/metabolismoRESUMEN
OBJECTIVE: To study the effects of yiqi huoxue recipe and Coxsackie B virus type 3 (CVB3) on the expression of ribosomal protein S20 in rat cardiac myocytes, to explore the pathogenesis of myocarditis induced by CVB3 and the mechanism of yiqi huoxue recipe on gene level, and to further investigate whether yiqi huoxue recipe is an effective prescription for CVB3 myocarditis. METHODS: A modified suppression subtractive hybridization (SSH) was used to isolate differentially expressed genes between the CVB3 infection group and the treatment group with yiqi huoxue recipe. The results were further verified by fluorescence RT-PCR. RESULTS: The results of SSH showed that the gene expression of ribosomal protein S20 in the treatment group was higher than that in the CVB3 infection group (P<0.05), which agreed with the results of fluorescent RT-PCR. CONCLUSION: Down-regulation of ribosomal protein S20 mRNA expression might be one of the mechanisms in CVB3 myocarditis, and yiqi huoxue recipe could achieve the treatment of viral myocarditis by regulating the expression of ribosomal protein S20.
Asunto(s)
Medicamentos Herbarios Chinos/farmacología , Enterovirus Humano B/efectos de los fármacos , Enterovirus Humano B/metabolismo , Miocitos Cardíacos/metabolismo , Miocitos Cardíacos/virología , Proteínas Ribosómicas/metabolismo , Animales , Infecciones por Coxsackievirus/tratamiento farmacológico , Infecciones por Coxsackievirus/virología , Medicamentos Herbarios Chinos/uso terapéutico , Regulación de la Expresión Génica/efectos de los fármacos , Miocitos Cardíacos/efectos de los fármacos , Hibridación de Ácido Nucleico , Ratas , Ratas Wistar , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Proteínas Ribosómicas/genéticaRESUMEN
The ethics of Western medicine and that of traditional Chinese medicine (TCM) developed separately in their own ways. The formation and development of ancient medical ethics of China were extensively and deeply influenced by Confucianism, Taoism, Buddhism and other religious thought, while the ancient ethic basis of western society was influenced by traditional Judaism, Christianism, Catholicism and other natural philosophical thinking of ancient Greece and Rome. With the progress of medical and life sciences, the medical ethics begins to transfer into the life ethics, thus giving rise to new questions in the ethics of Western medicine and TCM.