RESUMEN
Staphylococcus aureus (S. aureus) can evade antibiotics and host immune defenses by persisting within infected cells. Here, we demonstrate that in infected host cells, S. aureus type VII secretion system (T7SS) extracellular protein B (EsxB) interacts with the stimulator of interferon genes (STING) protein and suppresses the inflammatory defense mechanism of macrophages during early infection. The binding of EsxB with STING disrupts the K48-linked ubiquitination of EsxB at lysine 33, thereby preventing EsxB degradation. Furthermore, EsxB-STING binding appears to interrupt the interaction of 2 vital regulatory proteins with STING: aspartate-histidine-histidine-cysteine domain-containing protein 3 (DHHC3) and TNF receptor-associated factor 6. This persistent dual suppression of STING interactions deregulates intracellular proinflammatory pathways in macrophages, inhibiting STING's palmitoylation at cysteine 91 and its K63-linked ubiquitination at lysine 83. These findings uncover an immune-evasion mechanism by S. aureus T7SS during intracellular macrophage infection, which has implications for developing effective immunomodulators to combat S. aureus infections.
Asunto(s)
Proteínas Bacterianas , Macrófagos , Proteínas de la Membrana , Infecciones Estafilocócicas , Staphylococcus aureus , Sistemas de Secreción Tipo VII , Ubiquitinación , Staphylococcus aureus/inmunología , Proteínas de la Membrana/metabolismo , Proteínas de la Membrana/inmunología , Humanos , Proteínas Bacterianas/metabolismo , Proteínas Bacterianas/inmunología , Macrófagos/inmunología , Macrófagos/metabolismo , Macrófagos/microbiología , Animales , Infecciones Estafilocócicas/inmunología , Infecciones Estafilocócicas/microbiología , Infecciones Estafilocócicas/metabolismo , Sistemas de Secreción Tipo VII/metabolismo , Sistemas de Secreción Tipo VII/inmunología , Sistemas de Secreción Tipo VII/genética , Ratones , Evasión Inmune , Interacciones Huésped-Patógeno/inmunologíaRESUMEN
Since the initial introduction of whole-cell bioreporters (WCBs) nearly 30 years ago, their high sensitivity, selectivity, and suitability for on-site detection have rendered them highly promising for environmental monitoring, medical diagnosis, food safety, biomanufacturing, and other fields. Especially in the environmental field, the technology provides a fast and efficient way to assess the bioavailability of pollutants in the environment. Despite these advantages, the technology has not been commercialized. This lack of commercialization is confusing, given the broad application prospects of WCBs. Over the years, numerous research papers have focused primarily on enhancing the sensitivity and selectivity of WCBs, with little attention paid to their wider commercial applications. So far, there is no a critical review has been published yet on this topic. Therefore, in this article we critically reviewed the research progress of WCBs over the past three decades, assessing the performance and limitations of current systems to understand the barriers to commercial deployment. By identifying these obstacles, this article provided researchers and industry stakeholders with deeper insights into the challenges hindering market entry and inspire further research toward overcoming these barriers, thereby facilitating the commercialization of WCBs as a promising technology for environmental monitoring.
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The carbonization of lignocellulosic biomass with ionic liquids (ILs) are considered as an advantageous approach for the preparation of carbonaceous materials. The commonly used imidazolium and pyridinium based ILs have drawbacks such as toxicity, resistance to biodegradation, high cost and viscosity. These issues can be mitigated by diluting ILs with water, although excessive water content above 1 wt% can reduce the solubility of biomass. This research aims to investigate the potential of pretreating wastepaper with a "fully green" ILs, amino acid-based IL with high water content, followed by pyrolysis without IL, in enhancing the properties of biochar. For this purpose, the paper was treated with an aqueous solution of IL cysteine nitrate ([Cys][NO3]), and the IL was not involved in the pyrolysis process to prevent the formation of secondary gaseous pollutants. The findings revealed that the hemicellulose and mineral filler in the paper were eliminated during pretreatment, leading to higher carbon content but lower oxygen content. As a result, the biochar exhibited micropores of 0.42 cm3g-1 and a specific surface area of 1011.21 m2 g-1. The biochar demonstrated high adsorption capacities for Cd2+, enrofloxacin, bisphenol A, ciprofloxacin, and tetracycline, with values of 45.20 mg g-1, 49.82 mg g-1, 49.90 mg g-1, 49.88 mg g-1, and 49.65 mg g-1, respectively. The proposed mechanism for the adsorption of enrofloxacin by the biochar primarily involves physical adsorption such as pore filling and electrostatic interactions, along with chemical adsorption facilitated by graphitic nitrogen.
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Aminoácidos , Carbón Orgánico , Líquidos Iónicos , Líquidos Iónicos/química , Carbón Orgánico/química , Adsorción , Aminoácidos/químicaRESUMEN
Biodegradable plastics (bio-plastics) are often viewed as viable option for mitigating plastic pollution. Nevertheless, the information regarding the potential risks of microplastics (MPs) released from bio-plastics in soil, particularly in flooded soils, is lacking. Here, our objective was to investigate the effect of polylactic acid MPs (PLA-MPs) and polyethylene MPs (PE-MPs) on soil properties, microbial community and plant growth under both non-flooded and flooded conditions. Our results demonstrated that PLA-MPs dramatically increased soil labile carbon (C) content and altered its composition and chemodiversity. The enrichment of labile C stimulated microbial N immobilization, resulting in a depletion of soil mineral nitrogen (N). This specialized environment created by PLA-MPs further filtered out specific microbial species, resulting in a low diversity and simplified microbial community. PLA-MPs caused an increase in denitrifiers (Noviherbaspirillum and Clostridium sensu stricto) and a decrease in nitrifiers (Nitrospira, MND1, and Ellin6067), potentially exacerbating the mineral N deficiency. The mineral N deficit caused by PLA-MPs inhibited wheatgrass growth. Conversely, PE-MPs had less effect on soil ecosystems, including soil properties, microbial community and wheatgrass growth. Overall, our study emphasizes that PLA-MPs cause more adverse effect on the ecosystem than PE-MPs in the short term, and that flooded conditions exacerbate and prolong these adverse effects. These results offer valuable insights for evaluating the potential threats of bio-MPs in both uplands and wetlands.
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Inundaciones , Microbiota , Microplásticos , Microbiología del Suelo , Contaminantes del Suelo , Suelo , Microplásticos/toxicidad , Suelo/química , Microbiota/efectos de los fármacos , Plásticos Biodegradables , Desarrollo de la Planta , Biodegradación Ambiental , Poliésteres , PolietilenoRESUMEN
Biochar's interaction with soil-dwelling organisms, particularly earthworms, is crucial in ensuring the effective and secure utilization of biochar in the soil. This review introduces the application of biochar in soil, summarizes how earthworms respond to biochar-amended soil and the underlying factors that can influence their response, discusses the synergistic and antagonistic impacts of earthworm activity on the efficacy of biochar, and considers the feasibility of applying them together. A review of existing research has identified uncertainty in the effect of biochar exposure on earthworms, with biochar derived from animal wastes, produced at higher pyrolysis temperatures, and used at higher doses of biochar having more negative effects on earthworms. Habitat modification, toxicity release, particle effects, and contaminant immobilization are underlying factors in how biochar affects earthworm indicators. While biochar in contaminated soils may alleviate the stress of pollutants on earthworms by decreasing their bioaccumulation, this remedial effect is not always effective. Additionally, earthworm bioturbation can enhance the migration, fragmentation, and oxidation of biochar, while also stimulating extracellular enzymes that convert biochar into 'vermichar'. Earthworms and biochar can synergize well to improve soil fertility and remediate soil organic pollution, yet exhibit contrasting roles in soil C sequestration and immobilizing heavy metals in soil. These findings highlight both the advantages and risks of their co-application. Therefore, when considering the use of biochar alone or with earthworms, it is crucial to thoroughly assess its potential ecotoxicity on earthworms and other soil organisms, as well as the influence of bioturbation, such as that caused by earthworms, on the effectiveness of biochar.
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Low-carbon alkaline thermal hydrolysis of sewage sludge for the production of high-quality plant-growth-promoting nutrients and biostimulants is a growing concern for sludge resource utilization in agriculture. Thus, this study aims to investigate functional characteristics and soil biochemical effects of sewage sludge-derived nutrients and biostimulants (SS-NB). The content of heavy metals in SS-NB decreased by 47.39-100%, and an increase in soil protease, invertase, and soil nutrient utilization rates are observed in SS-NB groups. SS-NB substituted for chemical fertilizer increased the diversity and evenness of microbial community and reduced the abundance of the soil-borne bacterial genus Arthrobacter. The dominant community of SS-NB100 group is mainly enriched in Microvirga, Ensifer, Novosphingobium, Bosea and Ellin6055, which are principally beneficial symbiotic bacteria of plants and participated in C and N cycles. Moreover, SS-NB reduced the accumulation of Ktedonobacteria and Nitrosospira, which are involved in the production of CO2 and N2O, and also enhanced the coordination of soil microorganisms with enzyme activities and nutrient utilization rate. In conclusion, the results suggest that SS-NB exerts a positive effect on reducing greenhouse gas emissions and preventing soil-borne diseases, and can further enhance collaboration with soil enzyme activity and soil nutrient utilization by stimulating soil microorganisms.
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Aguas del Alcantarillado , Suelo , Suelo/química , Aguas del Alcantarillado/química , Hidrólisis , Agricultura , NutrientesRESUMEN
Cardiac fibroblasts (CFs) are the primary cells tasked with depositing and remodeling collagen and significantly associated with heart failure (HF). TEAD1 has been shown to be essential for heart development and homeostasis. However, fibroblast endogenous TEAD1 in cardiac remodeling remains incompletely understood. Transcriptomic analyses revealed consistently upregulated cardiac TEAD1 expression in mice 4 weeks after transverse aortic constriction (TAC) and Ang-II infusion. Further investigation revealed that CFs were the primary cell type expressing elevated TEAD1 levels in response to pressure overload. Conditional TEAD1 knockout was achieved by crossing TEAD1-floxed mice with CFs- and myofibroblasts-specific Cre mice. Echocardiographic and histological analyses demonstrated that CFs- and myofibroblasts-specific TEAD1 deficiency and treatment with TEAD1 inhibitor, VT103, ameliorated TAC-induced cardiac remodeling. Mechanistically, RNA-seq and ChIP-seq analysis identified Wnt4 as a novel TEAD1 target. TEAD1 has been shown to promote the fibroblast-to-myofibroblast transition through the Wnt signalling pathway, and genetic Wnt4 knockdown inhibited the pro-transformation phenotype in CFs with TEAD1 overexpression. Furthermore, co-immunoprecipitation combined with mass spectrometry, chromatin immunoprecipitation, and luciferase assays demonstrated interaction between TEAD1 and BET protein BRD4, leading to the binding and activation of the Wnt4 promoter. In conclusion, TEAD1 is an essential regulator of the pro-fibrotic CFs phenotype associated with pathological cardiac remodeling via the BRD4/Wnt4 signalling pathway.
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Factores de Transcripción de Dominio TEA , Factores de Transcripción , Remodelación Ventricular , Animales , Ratones , Miofibroblastos/metabolismo , Miofibroblastos/patología , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Factores de Transcripción de Dominio TEA/genética , Factores de Transcripción de Dominio TEA/metabolismo , Factores de Transcripción/genética , Remodelación Ventricular/genética , Proteína Wnt4/metabolismo , Fibroblastos/metabolismo , Proteínas que Contienen Bromodominio/metabolismoRESUMEN
Per- and polyfluoroalkyl substances (PFAS) present significant environmental and health hazards due to their inherent persistence, ubiquitous presence in the environment, and propensity for bioaccumulation. Consequently, the development of efficacious remediation strategies for soil and water contaminated with PFAS is imperative. Biochar, with its unique properties, has emerged as a cost-effective adsorbent for PFAS. Despite this, a comprehensive review of the factors influencing PFAS adsorption and immobilization by biochar is lacking. This narrative review examines recent findings indicating that the application of biochar can effectively immobilize PFAS, thereby mitigating their environmental transport and subsequent ecological impact. In addition, this paper reviewed the sorption mechanisms of biochar and the factors affecting its sorption efficiency. The high effectiveness of biochars in PFAS remediation has been attributed to their high porosity in the right pore size range (>1.5 nm) that can accommodate the relatively large PFAS molecules (>1.02-2.20 nm), leading to physical entrapment. Effective sorption requires attraction or bonding to the biochar framework. Binding is stronger for long-chain PFAS than for short-chain PFAS, as attractive forces between long hydrophobic CF2-tails more easily overcome the repulsion of the often-anionic head groups by net negatively charged biochars. This review summarizes case studies and field applications highlighting the effectiveness of biochar across various matrices, showcasing its strong binding with PFAS. We suggest that research should focus on improving the adsorption performance of biochar for short-chain PFAS compounds. Establishing the significance of biochar surface electrical charge in the adsorption process of PFAS is necessary, as well as quantifying the respective contributions of electrostatic forces and hydrophobic van der Waals forces to the adsorption of both short- and long-chain PFAS. There is an urgent need for validation of the effectiveness of the biochar effect in actual environmental conditions through prolonged outdoor testing.