WeChat Continuity Nursing on Postpartum Depression and Quality of Life in Primipara Undergoing Cesarean Delivery.

Objective: To assess the effectiveness of WeChat-based continuity nursing in reducing postpartum depression and improving the quality of life among primiparous women undergoing cesarean delivery. Methods: A total of 200 patients who intended to undergo cesarean delivery in our hospital between January 2021 and January 2022 were recruited for this study, including 20 patients who refused to participate in the study and 30 patients who did not meet the criteria of this study for various reasons, and a total of 150 cases were finally included. All participants were assigned 1:1 into the control group and observation group according to the time of the first pregnancy test and the primiparous in the observation group were given WeChat continuity nursing, and the primiparous in the control group was given routine obstetric care. WeChat continuity nursing included establishment of continuity nursing team, WeChat group setup and communication, education and support, and psychological counseling and follow-up. The baseline data of all mothers were collected and recorded, and the Self-Rating Anxiety Scale (SAS) and Self-Rating Depression Scale (SDS), quality of life scores, maternal and infant complications, and the satisfaction rate of care were compared between the two groups. Results: The SAS and SDS scores of the observation group were consistently lower than those of the control group at 1, 3, and 6 months post-hospital discharge (P < .01). Following the implementation of WeChat continuity nursing intervention, patients in the observation group demonstrated significant improvements in mental health, physical function, somatic pain, vitality, and social function scores compared to the control group (P < .01). Additionally, the incidence of complications was notably lower in the observation group, including reduced rates of incisional infection, breast swelling, unclear dew, abnormal defecation among mothers, and decreased occurrence of breech redness, umbilical cord issues, eczema, and delayed umbilical cord detachment among infants (P < .05). Moreover, the satisfaction rate among patients in the observation group was significantly higher than that of the control group (95.507% vs. 84.058%) (P < .05). These findings highlight the efficacy and importance of integrating WeChat continuity nursing intervention into postpartum care practices. Conclusion: Our study strongly supports the effectiveness of WeChat continuity nursing intervention in improving postpartum mental health, reducing complications, and increasing patient satisfaction. These findings suggest the potential for integrating digital nursing interventions into standard postpartum care practices, offering personalized and accessible healthcare services. Policymakers and healthcare providers should consider adopting such interventions to optimize postpartum outcomes and enhance patient experiences.

The Effect of Hispidulin, a Flavonoid from Salvia plebeia, on Human Nasopharyngeal Carcinoma CNE-2Z Cell Proliferation, Migration, Invasion, and Apoptosis.

Nasopharyngeal carcinoma (NPC) is a common malignant head and neck tumor. Drug resistance and distant metastasis are the predominant cause of treatment failure in NPC patients. Hispidulin is a flavonoid extracted from the bioassay-guided separation of the EtOH extract of Salvia plebeia with strong anti-proliferative activity in nasopharyngeal carcinoma cells (CNE-2Z). In this study, the effects of hispidulin on proliferation, invasion, migration, and apoptosis were investigated in CNE-2Z cells. The [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assay and the colony formation assay revealed that hispidulin could inhibit CNE-2Z cell proliferation. Hispidulin (25, 50, 100 µM) also induced apoptosis in a dose-dependent manner in CNE-2Z cells. The expression of Akt was reduced, and the expression of the ratio of Bax/Bcl-2 was increased. In addition, scratch wound and transwell assays proved that hispidulin (6.25, 12.5, 25 µM) could inhibited the migration and invasion in CNE-2Z cells. The expressions of HIF-1α, MMP-9, and MMP-2 were decreased, while the MMPs inhibitor TIMP1 was enhanced by hispidulin. Moreover, hispidulin exhibited potent suppression tumor growth and low toxicity in CNE-2Z cancer-bearing mice at a dosage of 20 mg/kg/day. Thus, hispidulin appears to be a potentially effective agent for NPC treatment.

Prediction of genomic breeding values based on pre-selected SNPs using ssGBLUP, WssGBLUP and BayesB for Edwardsiellosis resistance in Japanese flounder.

BACKGROUND: Edwardsiella tarda causes acute symptoms with ascites in Japanese flounder (Paralichthys olivaceus) and is a major problem for China's aquaculture sector. Genomic selection (GS) has been widely adopted in breeding industries because it shortens generation intervals and results in the selection of individuals that have great breeding potential with high accuracy. Based on an artificial challenge test and re-sequenced data of 1099 flounders, the aims of this study were to estimate the genetic parameters of resistance to E. tarda in Japanese flounder and to evaluate the accuracy of single-step GBLUP (ssGBLUP), weighted ssGBLUP (WssGBLUP), and BayesB for improving resistance to E. tarda by using three subsets of pre-selected single nucleotide polymorphisms (SNPs). In addition, SNPs that are associated with this trait were identified using a single-SNP genome-wide association study (GWAS) and WssGBLUP. RESULTS: We estimated a heritability of 0.13 ± 0.02 for resistance to E. tarda in Japanese flounder. One million SNPs at fixed intervals were selected from 4,978,724 SNPs that passed quality controls. GWAS identified significant SNPs on chromosomes 14 and 24. WssGBLUP revealed that the putative quantitative trait loci on chromosomes 1 and 14 contained SNPs that explained more than 1% of the genetic variance. Three 50 k-SNP subsets were pre-selected based on different criteria. Compared with pedigree-based prediction (ABLUP), the three genomic methods evaluated resulted in at least 7.7% greater accuracy of predictions. The accuracy of these genomic prediction methods was almost unchanged when pre-selected trait-related SNPs were used for prediction. CONCLUSIONS: Resistance to E. tarda in Japanese flounder has a low heritability. GWAS and WssGBLUP revealed that the genetic architecture of this trait is polygenic. Genomic prediction of breeding values performed better than ABLUP. It is feasible to implement genomic selection to increase resistance to E. tarda in Japanese flounder with 50 k SNPs. Based on the criteria used here, pre-selection of SNPs was not beneficial and other criteria for pre-selection should be considered.

[Analysis on utility and efficacy of fiberoptic bronchoscopy in intensive care unit].

OBJECTIVE: To evaluate the utility and safety of fiberoptic bronchoscopy (FOB) in an intensive care unit (ICU). METHODS: From Aug 2013 to Aug 2014, out of 492 patients admitted into Beijing Haidian Hospital ICU, 86 patients were included according to the study. Data including age, sex, APACHE, SAPS and Disease type, were processed using SPSS16.0. RESULTS: A total of 104 FOBs were performed in 86 patients P who were admitted to the ICU. FOB was useful in 64% of diagnostic procedures and 81% of therapeutic procedures, with an overall utility of 72%. The utility according to pneumonia in 32 cases, utility of 51%; percutaneous tracheostomy guidance in 26 cases, utility of 100%; atelectasis in 20 cases, utility of 75%; airway exploration in 18 cases, utility of 75%; hemoptysis in 3 cases, utility of 100%; and difficult airway intubation in 5 cases, utility of 100%. A decrease in pulse-oximeter oxygen saturation (SpO2) of >5% during FOB was present in 65% cases. Mean SpO2 before FOB was 94.0%±5.2%. During the procedure, the mean lowest SpO2 was 86.8%±8.4% (P<0.000 1) and the mean postprocedural SpO2 was 93.0%±4.5% (P=0.82). The other minor complications were present in 3.9% of cases. CONCLUSIONS: FOB has a great application field for the patients in ICU. The utility and safety of this operation is credible.

Isolation and functional characterization of a novel rice constitutive promoter.

KEY MESSAGE: A novel rice constitutive promoter (P OsCon1 ) was isolated. The molecular mechanism of the promoter activity was investigated. P OsCon1 could be used as an alternative constitutive promoter for crop transgenic engineering. Monocot constitutive promoter is an important resource for crop transgenic engineering. In this report, we isolated a novel promoter, Oscon1 promoter (P OsCon1 ), from the 5' upstream region of a constitutively expressed rice gene OsDHAR1. In P OsCon1 ::GUS transgenic rice, we showed that P OsCon1 had a broad expression spectrum in all tested tissues. The expression of the promoter was further analyzed in comparison with the previously characterized strong constitutive promoters. P OsCon1 exhibited comparable activity to OsCc1, OsAct1 or ZmUbi promoters in most tissues, and more active than 35S promoter in roots, seeds, and calli. Further quantitative assays indicated that P OsCon1 activity was not affected by developmental stages or by environmental factors. Further, 5'-deletions analysis indicated that the distinct regions might contribute to the strong expression of P OsCon1 in different tissues. Overall, our results suggest that P OsCon1 is a novel constitutive promoter, which could potentially use in transgenic crop development.

MTHFR gene polymorphisms in diabetes mellitus.

The methylenetetrahydrofolate reductase (MTHFR), methionine synthase (MTR), and methionine synthase reductase (MTRR) are three regulatory enzymes in the folic acid (FA) cycle play a critical role in the balance of methionine and homocysteine. MTHFR and MTRR gene polymorphisms affect the biochemical activities of enzymes, impairing the remethylation of homocysteine to methionine. In 1972, severe MTHFR deficiency resulting in homocystinuria was first reported, suggesting MTHFR involvement in the disease. MTHFR C677T polymorphism can independently increase the risk of high homocysteine (HHcy) in plasma. Elevation of homocysteine levels could increase the risk of microvascular damage, thrombosis, heart disease, etc. Vascular complications were regarded as a leading major cause of diabetes mortality, and disability increases individual health and economic burden. Diabetes mellitus (DM) is a chronic inflammatory disease, and conventional medications do not provide a complete cure for diabetes. It was essential to identify other risk factors for the intervention and prevention of diabetes. MTHFR gene polymorphism is an emerging risk factor in diabetes. Recent studies have shown that polymorphisms of the MTHFR gene play a significant role in the pathophysiology of diabetes, including inflammation and insulin resistance. This review summarizes the association between MTHER gene polymorphism and diabetes.

Identification and Expression Analysis of Wnt2 Gene in the Sex Differentiation of the Chinese Soft-Shelled Turtle (Pelodiscus sinensis).

The Chinese soft-shelled turtle (Pelodiscus sinensis) is an important freshwater aquaculture animal in China. The Wnt gene family plays important regulatory roles in the development and growth of mammals. However, the precise function of these family genes has not been well understood in the sex differentiation of Chinese soft-shelled turtles. Here, we cloned a member of the Wnt family, Wnt2, which obtained a 1077 bp open reading frame that encoded a 358-aa protein. The putative amino acid sequences of proteins are exceeded 80% identical to other turtles. The expression level of Wnt2 peaked at the 14th stage both in female and male embryos during the early gonadal differentiation period of Chinese soft-shelled turtles, which occurred before gonadal differentiation. Wnt2 mRNA was expressed at higher levels in the brains and gonads of mature P. sinensis females compared with those in mature males. Wnt agonists significantly affected the expression level of Wnt2 during the gonadal differentiation period. After Wnt agonists (1.0 µg/µL, 2.5 µg/µL, 5.0 µg/µL) treatment, the expression level of the Wnt2 generally appeared to have an inverted-V trend over time in female embryonic gonads. The results suggested that Wnt2 may participate in the regulation of gonad development in P. sinensis during the early embryonic stages. These results could provide a theoretical basis for the reproduction process of the Chinese soft-shelled turtle.

Honokiol acts as an AMPK complex agonist therapeutic in non-alcoholic fatty liver disease and metabolic syndrome.

BACKGROUND: Non-alcoholic fatty liver (NAFLD) and its related metabolic syndrome have become major threats to human health, but there is still a need for effective and safe drugs to treat these conditions. Here we aimed to identify potential drug candidates for NAFLD and the underlying molecular mechanisms. METHODS: A drug repositioning strategy was used to screen an FDA-approved drug library with approximately 3000 compounds in an in vitro hepatocyte model of lipid accumulation, with honokiol identified as an effective anti-NAFLD candidate. We systematically examined the therapeutic effect of honokiol in NAFLD and metabolic syndrome in multiple in vitro and in vivo models. Transcriptomic examination and biotin-streptavidin binding assays were used to explore the underlying molecular mechanisms, confirmed by rescue experiments. RESULTS: Honokiol significantly inhibited metabolic syndrome and NAFLD progression as evidenced by improved hepatic steatosis, liver fibrosis, adipose inflammation, and insulin resistance. Mechanistically, the beneficial effects of honokiol were largely through AMPK activation. Rather than acting on the classical upstream regulators of AMPK, honokiol directly bound to the AMPKγ1 subunit to robustly activate AMPK signaling. Mutation of honokiol-binding sites of AMPKγ1 largely abolished the protective capacity of honokiol against NAFLD. CONCLUSION: These findings clearly demonstrate the beneficial effects of honokiol in multiple models and reveal a previously unappreciated signaling mechanism of honokiol in NAFLD and metabolic syndrome. This study also provides new insights into metabolic disease treatment by targeting AMPKγ1 subunit-mediated signaling activation.

Nifedipine plus Systematic Nursing on Pregnancy Outcomes in Patients with Gestational Hypertension.

Objective: To study the effect of nifedipine combined with systematic nursing on pregnancy outcomes in patients with gestational hypertension. Methods: According to the random number table method, 120 patients with gestational hypertension treated in our hospital were divided into the control group (60 cases) and observation group (60 cases). The control group was given magnesium sulfate treatment, the observation group was given magnesium sulfate combined with nifedipine, and the two groups of patients were given systematic nursing. The blood pressure levels, negative emotions, and pregnancy outcomes of the two groups of patients before and after treatment were compared. The blood pressure levels of the two groups before and after treatment were measured by the electronic sphygmomanometer, and the self-rating depression scale (SDS) and self-rating scale (SAS) were used to evaluate the depression and anxiety of the two groups, respectively. Results: After treatment, the systolic blood pressure, diastolic blood pressure, SDS score, and SAS score of the two groups were decreased, and the observation group was significantly lower than the control group. Additionally, the observation group had lower incidence of cesarean section, postpartum hemorrhage, premature rupture of membranes, and neonatal asphyxia (P < 0.05). Conclusion: Nifedipine plus systematic nursing in patients with gestational hypertension can effectively improve the pregnancy outcome and adverse emotions such as depression and anxiety, reduce blood pressure, and promote disease recovery.

Boosting Photosynthetic H2O2 of Polymeric Carbon Nitride by Layer Configuration Regulation and Fluoride-Potassium Double-Site Modification.

Photocatalytic hydrogen peroxide (H2O2) production will become a burgeoning strategy for solar energy utilization by selective oxygen reduction reaction (ORR). Polymeric carbon nitride (PCN) shows relatively high two-electron ORR selectivity for H2O2 production but still limited low H2O2 production efficiency due to slow exciton dissociation. Herein, we constructed a heptazine/triazine layer stacked carbon nitride heterojunction with fluorine/potassium (F/K) dual sites (FKHTCN). The introduction of F/K not only can regulate layer components to enhance the charge separation efficiency but, more importantly, also optimize the adsorption of surface oxygen molecules and intermediate *OOH during H2O2 production. Consequently, FKHTCN efficiently improves the photocatalytic H2O2 production rate up to 3380.9 µmol h-1 g-1, nearly 15 times higher than that of traditional PCN. Moreover, a production-utilization cascade system was designed to explore their practical application in environmental remediation. This work lays out the importance of engineering a layer-stacked configuration and active sites for enhancing photocatalysis.

An injectable antibacterial chitosan-based cryogel with high absorbency and rapid shape recovery for noncompressible hemorrhage and wound healing.

Great challenges remain in the effective control of irregular and incompressible deep wound bleeding and the promotion of wound healing after bacterial infection. In this study, cryogels were prepared using an ice template based on chitosan (CS), oxidized gallic acid (OGA) and hemin (HE), which are all green edible materials. The cryogels exhibit rapid blood-triggered shape recovery and a high swelling ratio. The cryogels with 5 mg mL-1 HE (Gel-CS/OGA@HE5) exert excellent photothermal effects. Additionally, the cryogels have excellent cytocompatibility and blood clotting abilities. In the mouse liver injury model and mouse tail amputation model, Gel-CS/OGA@HE5 presents better hemostasis properties than gauze and a gelatin sponge. Moreover, Gel-CS/OGA@HE5 displays excellent healing performance as a wound dressing. Overall, we provide a simple and effective strategy to prepare cryogels for controlling wound bleeding and promoting wound healing.

Effect of the theory of planned behavior on primipara breastfeeding.

BACKGROUND: Breastfeeding is the most effective way to provide food for the healthy growth and development of babies. It has been reported in the literature that interventions on parturients, with the theory of planned behavior (TPB) as the guiding framework, can achieve good results. The purpose of the present study was to explore the effect of the TPB on primipara breastfeeding. METHODS: A total of 70 primiparas, who were given regular intervention mode at Haian People's Hospital from May 2017 to May 2018, were selected as the control group, and a total of 70 primiparas, who were given the TPB model from June 2018 to June 2019, were selected as the observation group. Exclusive breastfeeding rates, continuous breastfeeding rates, and breastfeeding confidence and breastfeeding impact factors were compared between the two groups. RESULTS: After the intervention, the pure breastfeeding rates of the observation group at discharge, 1 month after delivery, and 4 months after delivery were higher than those of the control group (P<0.05). After intervention, the continuous breastfeeding rates of the observation group was higher than that of the control group at 4 months after delivery (P<0.05). After intervention, the skill dimension, inner activity dimension, and total score of the observation group were higher than those of the control group (P<0.05). After intervention, scores of attitude, perceptual behavior control, and knowledge in the observation group were higher than those of the control group (P<0.05). CONCLUSIONS: The application of the TPB to primipara breastfeeding can significantly increase the rate of exclusive breastfeeding and continuous breastfeeding, and effectively improve the impact factors of breastfeeding.

Genomic Selection Using BayesCπ and GBLUP for Resistance Against Edwardsiella tarda in Japanese Flounder (Paralichthys olivaceus).

The Japanese flounder is one of the most widely farmed economic flatfish species throughout eastern Asia including China, Korea, and Japan. Edwardsiella tarda is a major species of pathogenic bacteria that causes ascites disease and, consequently, a huge economy loss for Japanese flounder farming. After generation selection, traditional breeding methods can hardly improve the E. tarda resistance effectively. Genomic selection is an effective way to predict the breeding potential of parents and has rarely been used in aquatic breeding. In this study, we chose 931 individuals from 90 families, challenged by E. tarda from 2013 to 2015 as a reference population and 71 parents of these families as selection candidates. 1,934,475 markers were detected via genome sequencing and applied in this study. Two different methods, BayesCπ and GBLUP, were used for genomic prediction. In the reference population, two methods led to the same accuracy (0.946) and Pearson's correlation results between phenotype and genomic estimated breeding value (GEBV) of BayesCπ and GBLUP were 0.912 and 0.761, respectively. In selection candidates, GEBVs from two methods were highly similar (0.980). A comparison of GEBV with the survival rate of families that were structured by selection candidates showed correlations of 0.662 and 0.665, respectively. This study established a genomic selection method for the Japanese flounder and for the first time applied this to E. tarda resistance breeding.

Numerical Simulation and Experimental Investigation of the Viscoelastic Heating Mechanism in Ultrasonic Plasticizing of Amorphous Polymers for Micro Injection Molding.

Ultrasonic plasticizing of polymers for micro-injection molding has been proposed and studied for its unique potential in materials and energy-saving. In our previous work, we have demonstrated the characteristics of the interfacial friction heating mechanism in ultrasonic plasticizing of polymer granulates. In this paper, the other important heating mechanism in ultrasonic plasticizing, i.e., viscoelastic heating for amorphous polymer, was studied by both theoretical modeling and experimentation. The influence mechanism of several parameters, such as the initial temperature of the polymer, the ultrasonic frequency, and the ultrasonic amplitude, was investigated. The results from both numerical simulation and experimentation indicate that the heat generation rate of viscoelastic heating can be significantly influenced by the initial temperature of polymer. The glass transition temperature was found to be a significant shifting point in viscoelastic heating. The heat generation rate is relatively low at the beginning and can have a steep increase after reaching glass transition temperature. In comparison with the ultrasonic frequency, the ultrasonic amplitude has much greater influence on the heat generation rate. In light of the quantitative difference in the viscoelastic heating rate, the limitation of the numerical simulation was discussed in the aspect of the assumptions and the applied mathematical models.

[Value of Neutrophil/Lymphocyte Ratio and Platelet/Lymphocyte Ratio for Prognostic Evaluation of Diffuse Large B-cell Lymphoma].

OBJECTIVE: To investigate the predictive value of neutrophil/lymphocyte ratio (NLR) and platelet/lymphocyte ratio (PLR) for the patients with diffuse large B-cell lymphoma (DLBCL). METHODS: The clinical data of 57 DLBCL patients admitted in the First Affiliated hospital of Anhui Medical University were analyzed retrospectively. According to ROC curve, the cut-off value for NLR and PLR was deterimined, and the patients were divided into high and low NLR/PLR groups before first chamotherapy. Then the relation of NLR and PLR with overall survival (OS) and progression-free survival (PFS) was analyzed by univariate and multivariate COX regression. RESULTS: The optimal cut-off value for NLR and PLR was 2.915 and 270.27, respectively. NLR at the diagnosis was found to be an independent predictor for OS and PFS by univariate and multivariate analysis, while the PLR was an independent predictor for PFS, but did not affect the OS. CONCLUSION: NLR and PLR may provide additional prognostic information for DLBCL patients.

A genome scan for quantitative trait loci associated with Vibrio anguillarum infection resistance in Japanese flounder (Paralichthys olivaceus) by bulked segregant analysis.

A recent genetic linkage map was employed to detect quantitative trait loci (QTLs) associated with Vibrio anguillarum resistance in Japanese flounder. An F1 family established and challenged with V. anguillarum in 2009 was used for QTL mapping. Of the 221 simple sequence repeat (SSR) markers used to detect polymorphisms in the parents of F1, 170 were confirmed to be polymorphic. The average distance between the markers was 10.6 cM. Equal amounts of genomic DNA from 15 fry that died early and from 15 survivors were pooled separately to constitute susceptible bulk and resistance bulk DNA. Bulked segregant analysis and QTL mapping were combined to detect candidate SSR markers and regions associated with the disease. A genome scan identified four polymorphic SSR markers, two of which were significantly different between susceptible and resistance bulk (P=0.008). These two markers were located in linkage group (LG) 7; therefore, all the SSR markers in LG7 were genotyped in all the challenged fry by single marker analysis. Using two different models, 11-17 SSR markers were detected with different levels of significance. To confirm the associations of these markers with the disease, composite interval mapping was employed to genotype all the challenged individuals. One and three QTLs, which explained more than 60 % of the phenotypic variance, were detected by the two models. Two of the QTLs were located at 48.6 cM. The common QTL may therefore be a major candidate region for disease resistance against V. anguillarum infection.

[Relationship between BIM gene polymorphism and therapeutic efficacy in the retreatment of advanced non-small cell lung cancer with tyrosine kinase inhibitor].

BACKGROUND AND OBJECTIVE: BIM gene is a member of the BCL-2 family, is involved in cell death. The aim of this study is to explore the relationship between BIM gene polymorphism and therapeutic efficacy in the retreatment advanced non-small cell lung cancer (NSCLC) with tyrosine kinase inhibitor (EGFR-TKI). METHODS: In the study, there were 123 patients who were diagnosed with advanced NSCLC in Zhejiang Province Cancer Hospital between January 2009 to October 2012, all of who were received gefitinib and erlotinib therapy after failure to chemotherapy. We detected the genotype of peripheral blood leukocytes of patients with BIM gene polymorphism though polymerase chain reaction (PCR). Statistical analysis was performed by SPSS version 13.0. RESULTS: On the disease control rates, BIM gene with no polymorphism type was slightly better trend than polymorphism types in disease control rate DCR (75.5% vs 57.1%, χ²=2.931, P=0.087). Univariate analysis the median PFS, women were longer than men (6.9 months vs 4.5 months, χ² =7.077, P=0.008). Non-smokers were longer than smokers (8.0 months vs 2.5 months, χ² =15.277, P<0.001). Adenocarcinoma were longer than others pathological type (7.0 months vs 2.0 months, χ² =14.978, P<0.001). The median PFS in BIM gene with no polymorphism type were longer than with polymorphism type (6.0 months vs 3.5 months, χ²=7.035, P=0.008). Multi-factor analysis showed that smoking, pathological type, the BIM gene polymorphism were the independent prognostic factors for PFS. CONCLUSIONS: The patients with the BIM gene no polymorphism have longer the median progression-free time than the polymorphism types in retreatment advanced non-small cell lung cancer patients with tyrosine kinase inhibitor.