RESUMEN
Renal cell carcinoma is one of the most universal urinary system cancers in the world. The most common renal cell carcinoma subtype is renal clear cell carcinoma. It is usually associated with high rates of metastasis and mortality. Therefore, finding effective therapeutic targets and prognostic molecular markers is of great significance to improve the early diagnosis rate and prognostic accuracy of renal clear cell carcinoma. In this work, we successfully identified six hub genes that are closely related to the occurrence, development and prognosis of renal clear cell carcinoma and proposed three new potential prognostic markers, namely ATP4B, AC144831.1 and Tfcp2l1 through differentially expressed genes (DEGs) analysis, GO functional enrichment and KEGG pathway analysis, WGCNA analysis, and survival analysis. In addition, we established machine learning models to predict the occurrence of tumors through the gene expression data of patients. It is expected that the results of this study can provide reference value for the treatment of renal clear cell carcinoma.
Asunto(s)
Carcinoma de Células Renales , Neoplasias Renales , Humanos , Carcinoma de Células Renales/genética , Carcinoma de Células Renales/metabolismo , Carcinoma de Células Renales/patología , Redes Reguladoras de Genes , Biomarcadores de Tumor/genética , Biomarcadores de Tumor/metabolismo , Regulación Neoplásica de la Expresión Génica , Neoplasias Renales/genética , Neoplasias Renales/patología , Aprendizaje AutomáticoRESUMEN
Understanding the interaction of T-cell receptor (TCR) with major histocompatibility-peptide (MHC-peptide) complex is extremely important in human immunotherapy and vaccine development. However, due to the limited available data, the performance of existing models for predicting the interaction of T-cell receptors (TCR) with major histocompatibility-peptide complexes is still unsatisfactory. Deep learning models have been applied to prediction tasks in various fields and have achieved better results compared with other traditional models. In this study, we leverage the gMLP model combined with attention mechanism to predict the interaction of MHC-peptide and TCR. Experiments show that our model can predict TCR-peptide interactions accurately and can handle the problems caused by different TCR lengths. Moreover, we demonstrate that the models trained with paired CDR3ß-chain and CDR3α-chain data are better than those trained with only CDR3ß-chain or with CDR3α-chain data. We also demonstrate that the hybrid model has greater potential than the traditional convolutional neural network.