IGF2 deficiency promotes liver aging through mitochondrial dysfunction and upregulated CEBPB signaling in D-galactose-induced aging mice.

BACKGROUND: Liver aging, marked by cellular senescence and low-grade inflammation, heightens susceptibility to chronic liver disease and worsens its prognosis. Insulin-like growth factor 2 (IGF2) has been implicated in numerous aging-related diseases. Nevertheless, its role and underlying molecular mechanisms in liver aging remain largely unexplored. METHODS: The expression of IGF2 was examined in the liver of young (2-4 months), middle-aged (9-12 months), and old (24-26 months) C57BL/6 mice. In vivo, we used transgenic IGF2f/f; Alb-Cre mice and D-galactose-induced aging model to explore the role of IGF2 in liver aging. In vitro, we used specific short hairpin RNA against IGF2 to knock down IGF2 in AML12 cells. D-galactose and hydrogen peroxide treatment were used to induce AML12 cell senescence. RESULTS: We observed a significant reduction of IGF2 levels in the livers of aged mice. Subsequently, we demonstrated that IGF2 deficiency promoted senescence phenotypes and senescence-associated secretory phenotypes (SASPs), both in vitro and in vivo aging models. Moreover, IGF2 deficiency impaired mitochondrial function, reducing mitochondrial respiratory capacity, mitochondrial membrane potential, and nicotinamide adenine dinucleotide (NAD)+/NADH ratio, increasing intracellular and mitochondrial reactive oxygen species levels, and disrupting mitochondrial membrane structure. Additionally, IGF2 deficiency markedly upregulated CCAAT/enhancer-binding protein beta (CEBPB). Notably, inhibiting CEBPB reversed the senescence phenotypes and reduced SASPs induced by IGF2 deficiency. CONCLUSIONS: In summary, our findings strongly suggest that IGF2 deficiency promotes liver aging through mitochondrial dysfunction and upregulated CEBPB signaling. These results provide compelling evidence for considering IGF2 as a potential target for interventions aimed at slowing down the process of liver aging.

Cognitive style modulates conscious but not unconscious thought: Comparing the deliberation-without-attention effect in analytics and wholists.

Unconscious thought theory (UTT) suggests that conscious thinking is less effective in complex decision-making than unconscious thinking. However, little research has taken individual differences (e.g., cognitive style) into account. Using an adapted UTT paradigm, the present study compared the performances of individuals with a wholist or an analytic cognitive style in both conscious and unconscious thought conditions. After viewing information regarding four hypothetical phones, participants in the conscious thought condition deliberated for three minutes before rating the phones, while participants in the unconscious thought condition were distracted with a 2-back task for three minutes before rating. The results showed that wholists were equally good at differentiating good and bad phones after conscious or unconscious thought, whereas analytics performed well only when thinking unconsciously. The modulation effect of cognitive style appeared only in conscious thought. Implications for UTT and the understanding of cognitive style are discussed.

Correlation of IGF2 levels with sperm quality, inflammation, and DNA damage in infertile patients.

Insulin-like growth factor 2 (IGF2) is a critical endocrine mediator implicated in male reproductive physiology. To investigate the correlation between IGF2 protein levels and various aspects of male infertility, specifically focusing on sperm quality, inflammation, and DNA damage, a cohort of 320 male participants was recruited from the Women's Hospital, Zhejiang University School of Medicine (Hangzhou, China) between 1st January 2024 and 1st March 2024. The relationship between IGF2 protein concentrations and sperm parameters was assessed, and Spearman correlation and linear regression analysis were employed to evaluate the independent associations between IGF2 protein levels and risk factors for infertility. Enzyme-linked immunosorbent assay (ELISA) was used to measure IGF2 protein levels in seminal plasma, alongside markers of inflammation (tumor necrosis factor-alpha [TNF-α] and interleukin-1ß [IL-1ß]). The relationship between seminal plasma IGF2 protein levels and DNA damage marker phosphorylated histone H2AX (γ-H2AX) was also explored. Our findings reveal that IGF2 protein expression decreased notably in patients with asthenospermia and teratospermia. Correlation analysis revealed nuanced associations between IGF2 protein levels and specific sperm parameters, and low IGF2 protein concentrations correlated with increased inflammation and DNA damage in sperm. The observed correlations between IGF2 protein levels and specific sperm parameters, along with its connection to inflammation and DNA damage, underscore the importance of IGF2 in the broader context of male reproductive health. These findings lay the groundwork for future research and potential therapeutic interventions targeting IGF2-related pathways to enhance male fertility.

Regulation mechanism and pathogenic role of lncRNA plasmacytoma variant translocation 1 (PVT1) in human diseases.

Plasmacytoma variant translocation 1 (PVT1) is a long non-coding RNA (lncRNA) gene identified as a recurrent breakpoint of Burkitt's lymphomas. Human PVT1 gene is located on region 8q24.21, a well-known cancer risk region, and encodes at least 26 linear ncRNA isoforms and 26 circular RNA isoforms, as well as 6 microRNAs. Several PVT1 functioning models have been reported recently such as competing endogenous RNA (ceRNA) activity and regulating protein stability of oncogenes, especially MYC oncogene. The promoter of PVT1 gene is a boundary element of tumor-suppressor DNA. CircPVT1 derived from PVT1 gene is also a critical non-coding oncogenic RNA. Although substantial advancements have been made in understanding the roles of PVT1 in cancer recently, the detailed mechanisms underlying its functions remain unclear. Herein, we summarize the recent progressions on the mechanisms underlying PVT1 regulated gene expression at different levels. We also discuss the interaction between lncRNA and protein, RNA and DNA, as well as the potential cancer therapy strategy by targeting these networks.

Carbon balance in an alpine steppe in the Qinghai-Tibet plateau.

Carbon fluxes were measured using a static chamber technique in an alpine steppe in the Qinghai-Tibet Plateau from July 2000 to July 2001. It was shown that carbon emissions decreased in autumn and increased in spring of the next year, with higher values in growth seasons than in winters. An exponential correlation (E(carbon)= 0.22(exp(0.09T) + ln(0.31P+ 1)), R(2)= 0.77, P < 0.001) was shown between carbon emissions and environmental factors such as temperature (T) and precipitation (P). Using the daily temperature (T) and total precipitation (R), annual carbon emission from soil to the atmosphere was estimated to be 79.6 g C/m(2), 46% of which was emitted by microbial respiration. Considering an average net primary production of 92.5 g C/m(2) per year within the 2 year experiment, alpine steppes can take up 55.9 g CO(2)-C/m(2) per year. This indicates that alpine steppes are a distinct carbon sink, although this carbon reservoir was quite small.

Carbon sequestration in two alpine soils on the Tibetan Plateau.

Soil carbon sequestration was estimated in a conifer forest and an alpine meadow on the Tibetan Plateau using a carbon-14 radioactive label provided by thermonuclear weapon tests (known as bomb-(14)C). Soil organic matter was physically separated into light and heavy fractions. The concentration spike of bomb-(14)C occurred at a soil depth of 4 cm in both the forest soil and the alpine meadow soil. Based on the depth of the bomb-(14)C spike, the carbon sequestration rate was determined to be 38.5 g C/m(2) per year for the forest soil and 27.1 g C/m(2) per year for the alpine meadow soil. Considering that more than 60% of soil organic carbon (SOC) is stored in the heavy fraction and the large area of alpine forests and meadows on the Tibetan Plateau, these alpine ecosystems might partially contribute to "the missing carbon sink".

Spatial variations in daily average CO2 concentrations above wetland surface of Xianghai National Nature Reserve, China.

Horizontal and vertical variations of daily average CO2 concentration above the wetland surface were studied in Xianghai National Nature Reserve of China in August, 2000. The primary purpose was to study spatial distribution characteristics of CO2 concentration on the four levels of height(0.1 m, 0.6 m, 1.2 m and 2 m) and compare the differences of CO2 concentration under different land covers. Results showed that daily average CO2 concentration above wetland surface in Xianghai National Natural Reserve was lower than that above other wetlands in northeast China as well as the worldwide average, suggesting that Xianghai wetland absorbed CO2 in August and acted as "sink" of CO2. The horizontal variations on the four levels of height along the latitude were distinct, and had the changing tendency of "decreasing after increasing" with the increase of height. The areas with obvious variations were consistent on different levels of height, and those with the highest variations appeared above surface of shore, sloping field, Typha wetland and Phragmites wetland; the vertical variations were greatly different, with the higher variations in Phragmites wetland and Typha wetland, and the lands near the shore and the sloping field with the lower variations. Spatial variations of daily average CO2 concentrations above wetland surface were affected by surface qualities and land covers.

The role of short daily hemodialysis in the control of hyperphosphatemia, secondary hyperparathyroidism and anemia.

BACKGROUND: Hyperphosphatemia, secondary hyperparathyroidism (SHPT) and anemia are common secondary complications in hemodialysis patients with end-stage renal disease (ESRD). Compared with conventional hemodialysis (CHD), short daily hemodialysis (sDHD) has been found to be more effective in patients with ESRD. The objective of this study was to determine whether sDHD could improve hyperphosphatemia, SHPT and anemia in patients with ESRD. METHODS: Twenty-seven patients (11 women and 16 men, 46.8 ± 13.4 years old) were switched from CHD to sDHD. All hematologic parameters were measured prior to the switch (baseline), at 3 months after the switch (sDHD(1)) and at 6 months after the switch (sDHD(2)). RESULTS: The serum phosphate decreased from 2.54 ± 0.32 mmol/L at baseline to 2.15 ± 0.36 mmol/L (p < 0.001) at sDHD(1) and 1.97 ± 0.33 mmol/L (p < 0.001) at sDHD(2). Calcium-phosphate product decreased from 5.18 ± 1.24 mmol(2)/L(2) at baseline to 4.20 ± 0.71 mmol(2)/L(2) (p < 0.001) at sDHD(1) and 4.02 ± 0.83 mmol(2)/L(2) (p < 0.001) at sDHD(2). The serum PTH levels decreased from 223.9 ± 124.7 pmol/L at baseline to 196.3 ± 101.3 pmol/L (p < 0.05) at sDHD(2). The hemoglobin concentration increased significantly from CHD to sDHD. However, the requirement for erythropoietin (EPO) dose decreased from 6847.8 ± 1057.3 u/week at baseline to 5869.6 ± 1094.6 u/week (p < 0.05) at sDHD(2). CONCLUSIONS: sDHD may decrease serum phosphate, calcium-phosphate product and PTH, increase hemoglobin levels and decrease exogenous EPO dose requirements compared with CHD in hemodialysis patients.

[Estimation of net primary productivity in middle reaches of Yarlung Zangbo River and its two tributaries].

Based on the MODIS data and field measurement, the net primary production (NPP) in 2000 and 2006 in the middle reaches of Yarlung Zangbo River and its two tributaries in Tibet were estimated by using a mathematic model. The results showed that in study area, the NPP decreased gradually from valley to ridge, which was accorded with the gradients of precipitation and temperature. The annul NPP per unit area was averagely 86.8 g C m(-2) a(-1), and was 2.15 g C m(-2) a(-1) higher in 2006 than in 2000. Farmland ecosystem had the highest annual NPP per unit area (243.1 g C m(-2) a(-1)), while desert ecosystem had the lowest one (36.5 g C m(-2) a(-1)). The average total NPP in the two years was 512.8 x 10(10) g C a(-1), with the value in 2006 being 12.7 x 10(10) g C a(-1) higher than that in 2000. Meadow ecosystem had the highest annual total NPP (194.4 x 10(10) g C a(-1)), while desert ecosystem had the lowest one (30.3 x 10(10) g C a(-1)). In 2000-2006, the NPP value in the areas with strong human disturbance (0-4 km away from road) had a decreasing trend, while that in the areas difficult for human to reach was in adverse.

[Relationships between net primary production and nitrogen cycling in Chinese forest ecosystems].

In order to better understand the relationships between net primary production (NPP) of forest ecosystem and nitrogen cycling, we studied and analyzed the relationships between NPP, litter nitrogen, plant nitrogen retention, and soil net nitrogen mineralization rate in Chinese forest ecosystems. The results indicated that NPP was linearly and positively related to litter nitrogen, plant nitrogen retention and soil net nitrogen mineralization rate, with the most correlation with soil net nitrogen mineralization rate (R2 = 0.74, n = 37), and the next with plant nitrogen retention (R2 = 0.60, n = 37).