Recent progress towards industrial rhamnolipids fermentation: Process optimization and foam control.

The global market for rhamnolipids production holds great promise, and is in need of an economically viable mass-production scheme. Accordingly, several strategies have been employed to improve the efficiency of rhamnolipid production in the past few decades. Currently, rhamnolipids can be produced by Pseudomonas aeruginosa at a high yield (over 70 g/L) when vegetable oil is used as the carbon source under optimized fed-batch cultivations. However, severe foaming during rhamnolipid fermentation inhibits scaling-up and production efficiency. Stop valve was found to effective break the extremely stable rhamnolipids foams during fermentation, and production efficiency of rhamnolipids was highly improved, while its scale-up mechanism needs further study. In addition, the combination of both chemical and mechanical approaches is likely to be more efficiently resolving the foam problem existed in rhamnolipids fermentation than either chemical or mechanical methods alone.

Application of biosurfactant surfactin as a pH-switchable biodemulsifier for efficient oil recovery from waste crude oil.

Efficient oil separation is the most desirable, but still challenging solution for the waste crude oil problem. This study developed biosurfactant surfactin as a novel pH-switchable biodemulsifier for efficient oil separation. As found, surfactin demulsification achieved a quite well oil separation ratio of over 95% on model emulsions after 20 min at 50 °C. The validity of this demulsification process should be mainly based on the readily lost stabilization ability of surfactin in emulsions triggered by acid addition. Then, surfactin (0.2 g/L) treatment with the aid of ethanol (2%) to improve its distribution could recover over 95% of oil from waste crude oil. After treated by surfactin, the separated oil phase contains tiny water (less than 0.5%) and thus can be reused for resource recycling to reach a compromised balance between satisfying the strict environmental regulations and decreasing the high treatment costs. Hence, in consideration of high demulsification efficiency, environmental-friendly properties and cost-efficiency, surfactin has a great potential for industrial applications for oil recovery from waste crude oil which is a severe problem presents in most of the petroleum-related factories.