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Sci Rep ; 11(1): 20281, 2021 10 13.
Artículo en Inglés | MEDLINE | ID: mdl-34645905

RESUMEN

Fungal infections represent a major global health problem affecting over a billion people that kills more than 1.5 million annually. In this study, we employed an integrative approach to reveal the landscape of the human immune responses to Candida spp. through meta-analysis of microarray, bulk, and single-cell RNA sequencing (scRNA-seq) data for the blood transcriptome. We identified across these different studies a consistent interconnected network interplay of signaling molecules involved in both Toll-like receptor (TLR) and interferon (IFN) signaling cascades that is activated in response to different Candida species (C. albicans, C. auris, C. glabrata, C. parapsilosis, and C. tropicalis). Among these molecules are several types I IFN, indicating an overlap with antiviral immune responses. scRNA-seq data confirmed that genes commonly identified by the three transcriptomic methods show cell type-specific expression patterns in various innate and adaptive immune cells. These findings shed new light on the anti-Candida immune response, providing putative molecular pathways for therapeutic intervention.


Asunto(s)
Candida albicans/inmunología , Candida glabrata/inmunología , Candida parapsilosis/inmunología , Candidiasis/inmunología , Candidiasis/microbiología , Transducción de Señal/inmunología , Antivirales/farmacología , Biología Computacional/métodos , Bases de Datos Genéticas , Perfilación de la Expresión Génica , Regulación Fúngica de la Expresión Génica , Humanos , Inmunidad , Inmunidad Innata , Interferones/metabolismo , RNA-Seq , Transcripción Genética , Transcriptoma
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