Antimicrobial activity of essential oils against biofilms formed in dental acrylic resin: a systematic review of in vitro studies.

This study aimed to answer the question formulated according to the PICO strategy: 'Which essential oils show antimicrobial activity against biofilms formed on dental acrylic resin?' composed by population (dental acrylic resin), intervention (application of essential oils), comparison (denture cleansers, antifungal drugs, chlorhexidine, and oral mouthwashes), and outcome (antibiofilm activity). In vitro experimental studies evaluating the activity of EOs on biofilm formed on acrylic resin were included. PRISMA guidelines were followed, and the search was performed in the PubMed, Science Direct, Embase, and Lilacs databases and in the gray literature using Google Scholar and ProQuest in December 2023. A manual search of the reference lists of the included primary studies was performed. Of the 1467 articles identified, 37 were selected for full-text reading and 12 were included. Twelve EOs were evaluated, of which 11 showed activity against Candida spp., 3 against Staphylococcus aureus, and 1 against Pseudomonas aeruginosa. The EOs of Cymbopogon citratus, Cinnamomum zeylanicum, and Cymbopogon nardus showed higher action than chlorhexidine, C. nardus higher than Listerine, C. citratus higher than nystatin, and Melaleuca alternifolia higher than fluconazole and nystatin. However, chlorhexidine was more effective than Lippia sidoides and Salvia officinalis, sodium hypochlorite was more effective than L. sidoides, nystatin was more effective than Zingiber officinale, Amphotericin B more effective than Eucalyptus globulus and M. alternifolia. In conclusion, the EOs of C. zeylanicum, C. citratus, C. nardus, and M. alternifolia showed antimicrobial activity to reduce biofilm on dental acrylic resin.

Microstructural characterization, mechanical and microbiological properties of acrylic resins added with reduced graphene oxide.

To evaluate the microstructural characterization, mechanical properties and antimicrobial activity of acrylic resins incorporated with different concentrations of reduced graphene oxide (rGO). Specimens were made of self-cured and heat-cured acrylic resins for the control group and concentrations of 0.5%, 1%, and 3%. The microstructural characterization was evaluated by scanning electron microscopy (SEM) and energy-dispersive X-ray (EDS). For mechanical testing, flexural strength, and Knoop hardness tests were performed. Microbiological evaluations were performed by colony forming units (CFU) analysis, tetrazolium salt reduction (XTT), and SEM images. The modified acrylic resins showed increased mechanical properties at low concentrations (p < 0.05) and with reduced S. mutans (p < 0.05). Reduced graphene oxide interfered with the mechanical performance and microbiological properties of acrylic resins depending on the concentration of rGO, and type of polymerization and microorganism evaluated. The incorporation of graphene compounds into acrylic resins is an alternative to improve the antimicrobial efficacy and performance of the material.

Incorporating versus coating antimicrobials for polymethyl methacrylate: A systematic review.

STATEMENT OF PROBLEM: Incorporating and coating with antimicrobials are techniques that can confer antimicrobial action on polymethyl methacrylate (PMMA) denture bases, which can accumulate microorganisms and promote oral and systemic disease. PURPOSE: The purpose of this systematic review was to answer the question: "Do techniques for incorporating and coating antimicrobial agents in PMMA promote antimicrobial action?" MATERIAL AND METHODS: The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist guidelines were followed, and the SCOPUS, PubMed/Medline, EMBASE, and Science Direct databases searched. The studies were selected in 2 stages, reading the titles and abstracts and then reading the selected studies in full. The risk of bias was analyzed by adapting the quasi-experimental studies tool by the Joanna Briggs Institute (JBI). RESULTS: A total of 970 articles were found in the databases; 71 were duplicates and, after reading the abstracts, 38 were selected for full reading. From these, 6 were excluded because they did not fulfill the inclusion criteria, and 32 studies were included in this review. Autopolymerizing, heat- polymerizing, and light-polymerizing resins were evaluated, with the incorporating technique prevailing over the coating, but both techniques effectively promoted antimicrobial activity. CONCLUSIONS: Incorporating and coating antimicrobial agents are effective methods of promoting antimicrobial activity in PMMA. Combining the 2 methods led to increased antimicrobial activity compared with each individually.

Denture stomatitis: Treatment with antimicrobial drugs or antifungal gels? A systematic review of clinical trials.

STATEMENT OF PROBLEM: Gel formulations containing antimicrobials are a potential alternative for the development of new medicines for the treatment of denture stomatitis (DS). However, whether they are more effective than antifungal drugs is unclear. PURPOSE: The purpose of this systematic review was to identify whether gels formulated with antimicrobial substances are more effective and biocompatible for the treatment of DS than antifungal drugs. MATERIAL AND METHODS: This systematic review was structured according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) and registered in the International Prospective Register of Systematic Reviews (PROSPERO; CRD42022379510). Searches were performed in the PubMed, Lilacs, Web of Science, Scopus, Embase, and Google Scholar databases in November 2022. Randomized and nonrandomized clinical trials comparing the efficacy of experimental gels and conventional antifungals in the treatment of DS were included. RESULTS: Gels formulated with Zataria multiflora essential oil and clove and cinnamon extracts showed higher antimicrobial efficacy compared with miconazole and clotrimazole. Formulations containing Uncaria tomentosa, Punica granatum, and propolis extract showed similar efficacy to nystatin and miconazole. Two formulations containing P. granatum were less effective than nystatin and miconazole. Gels of Z. multiflora and miconazole induced adverse effects including burning, itching, nausea, and vomiting. The included studies did not evaluate the biocompatibility of the gels. CONCLUSIONS: Gels formulated with natural antimicrobials such as Z. multiflora, U. tomentosa, P. granatum, propolis, and clove and cinnamon showed higher or similar antimicrobial efficacy to conventional antifungals in the treatment of DS. The biocompatibility of experimental gels based on natural agents should be evaluated.

Comparative analysis of the physical, chemical, and microbiological properties of Ti-6Al-4V disks produced by different methods and subjected to surface treatments.

STATEMENT OF PROBLEM: To improve the osseointegration of dental implants and reduce microbiological growth, different micro- and nanoscale surface topographies can be used. PURPOSE: The purpose of this in vitro study was to evaluate the influence of Ti-6Al-4V with 4 surfaces, machined (DU), machined+hydroxyapatite (DUHAp), machined+acid-alkali treatment (DUAA), and additive manufacturing (DMA), on the physical, chemical, and microbiological properties. MATERIAL AND METHODS: The topography of Ti-6Al-4V disks with the 4 surfaces was evaluated by scanning electron microscopy (SEM), the chemical composition by energy dispersive X-ray spectroscopy (EDS), and the crystalline structure by X-ray diffraction (XRD). Physical and chemical properties were analyzed by using wettability and surface free energy, roughness, and microbial adhesion against Staphylococcus aureus by colony forming units (CFU). One-way ANOVA analysis of variance and the Tukey multiple comparisons test were applied to evaluate the data, except CFU, which was submitted to the Kruskal-Wallis nonparametric test (α=.05). RESULTS: DU photomicrographs showed a topography characteristic of a polished machined surface, DUHAp and DUAA exhibited patterns corresponding to the surface modifications performed, and in DMA the presence of partially fused spherical particles was observed. The EDS identified chemical elements inherent in the Ti-6Al-4V, and the DUHAp and DUAA disks also had the ions from the treatments applied. XRD patterns revealed similarities between DU and DMA, as well as characteristic peaks of hydroxyapatite (HA) in the DUHAp disk and the DUAA. Compared with DU and DMA the DUHAp and DUAA groups showed hydrophilic behavior with smaller contact angles and higher surface free energy (P<.05). DMA showed a higher mean value of roughness, different from the others (P<.05), and a higher CFU for S. aureus (P=.006). CONCLUSIONS: DUHAp and DUAA showed similar behaviors regarding wettability, surface free energy, and bacterial adhesion. Among the untreated groups, DMA exhibited higher roughness, bacterial adhesion, and lower wettability and surface free energy.

Influence of Er:YAG laser irradiation on surface properties of Ti-6Al-4V machined and hydroxyapatite coated.

Surface treatment by laser irradiation can change the topography of titanium; however, little is known about the changes it causes when applied to other coatings. This study aimed to evaluate the influence of Er:YAG laser irradiation on the surface properties of titanium-aluminum-vanadium (Ti-6Al-4V) discs. Four Ti-6Al-4V surfaces were evaluated (n = 10): CON-control, machined without surface treatment; LT-machined + laser treatment; HA-hydroxyapatite coating; and LT-HA-hydroxyapatite coating + laser treatment. For the laser treatment, an Er:YAG laser with a wavelength of 2940 nm, a frequency of 10 Hz, and an energy density of 12.8 J/cm2 was used. The morphology of the coating was investigated by scanning electron microscopy and the surface composition by energy-dispersive X-ray spectroscopy. The influence of laser irradiation treatment on roughness and wettability was also evaluated. The Er:YAG laser promoted a significant reduction in the roughness Sa (p < 0.05) and in the contact angle (p = 0.002) of the LT surface compared to the CON surface. On the LT-HA surface, a significant decrease in roughness was observed only for the Rz parameter (p = 0.015) and an increase in the contact angle (p < 0.001) compared to the HA surface. The use of the Er:YAG laser with the evaluated parameters decreased the surface roughness and improved the wetting capacity of machined without surface treatment. In the group with hydroxyapatite coating, the laser influenced the surface roughness only for the parameter Rz and reduced their wetting capacity.

Analysis of the influence of surface treatment by high-power laser irradiation on the surface properties of titanium dental implants: A systematic review.

STATEMENT OF PROBLEM: High-power laser irradiation may be a promising treatment for titanium dental implant surfaces. However, systematic reviews of the influence of high-power laser irradiation on the different properties of titanium surfaces are lacking. PURPOSE: The purpose of this systematic review was to analyze the influence of surface treatment by high-power laser irradiation on the surface properties of titanium and its alloys. MATERIAL AND METHODS: The PubMed, LILACS, COCHRANE library, and Science Direct databases were searched, and articles published in the last 10 years were included. Of the 725 articles initially identified, 27 were selected after full reading and the application of inclusion and exclusion criteria. RESULTS: The studies evaluated showed that laser irradiation treatment, depending on the settings and parameters used, promoted changes in the surface properties of titanium. In general, lower speed and a higher number of scans increased roughness. Laser surface treatment promoted the inclusion of more oxygen and improved the wetting capacity of titanium. Additionally, laser treatment improved the adherence of coatings. CONCLUSIONS: Changes in the surface properties of titanium after laser treatment have been demonstrated. However, determining protocols with specific parameters is necessary to optimize the results.

Osseointegration in relation to drilling speed in the preparation of dental implants sites: A systematic review.

STATEMENT OF PROBLEM: The drilling speed used for preparing dental implants may affect bone-implant contact (BIC), implant stability quotient (ISQ), and bone area fraction occupancy (BAFO). Different rotational speeds and the presence or absence of irrigation during site preparation have been investigated, but an established protocol for achieving the best osseointegration results is lacking. PURPOSE: The purpose of this systematic review was to investigate the influence of drill rotational speed on bone drilling for dental implant placement and its relationship with osseointegration. MATERIAL AND METHODS: This review included the preferred reporting items for systematic reviews and meta-analyses (PRISMA) and was registered in the international prospective register of systematic reviews (PROSPERO) database. Electronic searches were performed in the MEDLINE (PubMed), Scopus, Science Direct, and Embase databases. The risk of bias was analyzed by using the systematic review center for laboratory animal experimentation (SYRCLE). RESULTS: A total of 1282 articles were found, and after removing duplicates and applying the eligibility criteria to in vivo articles on animals that addressed drilling speed and its relationship to osseointegration, 8 articles were selected for analysis. Of these, 5 articles showed no statistical differences, and 3 others showed significantly better osseointegration results by analyzing the parameters of BIC, BAFO, ISQs, and pull-out forces (PoFs). In all selected articles, high-speed drilling was performed with irrigation. CONCLUSIONS: Although drilling speed seems to affect bone perforation, no definitive protocol was found in the literature consulted. The results vary depending on the combination of different factors, including bone type, irrigation, and drilling speed.

Effect of surface properties of ceramic materials on bacterial adhesion: A systematic review.

PURPOSE: The objective of this systematic review was to describe studies that report on whether surface characteristics such as electrostatic charge, surface free energy, and surface topography promote influence on bacterial adhesion on ceramic surfaces. MATERIAL AND METHOD: Searches in the SCOPUS, PubMed/Medline, Web of Science, EMBASE, and Google Scholar databases were performed between December 2020 and January 2021 and updated in March 2021. In addition, a manual search of reference lists from relevant retrieved articles was performed. The criteria included: studies that evaluated ceramic surfaces, which described factors such as surface free energy, electrostatic charges, roughness, zeta potential, and their relationship with bacteria. RESULTS: Database search resulted in 348 papers. Of the 24 studies selected for full reading, 17 articles remained in this systematic review. Another five studies were found in references of articles included, totaling 22 studies. These had a high heterogeneity making it difficult to perform statistical analysis, so a descriptive analysis was performed. CONCLUSIONS: For dental ceramics, not enough results were found to demonstrate the influence of the electrostatic condition, and its relationship with bacterial adhesion. However, studies of this review show that there is a correlation between bacterial adhesion, surface free energy, and topography. CLINICAL SIGNIFICANCE: The knowledge of ceramics with repulsive physical-chemical interactions would allow an environment suggestive of non-adhesion of pathogenic biofilm.

In vitro assessment of polyetheretherketone for an attachment component for an implant-retained overdenture.

STATEMENT OF PROBLEM: Frequent maintenance because of the limited lifetime of overdenture attachments with O-rings has led to the development of materials that might improve their functionality and longevity. However, testing of newly developed attachment materials is lacking. PURPOSE: The purpose of this in vitro study was to evaluate a newly developed attachment made of polyetheretherketone (PEEK) for an implant-retained overdenture. MATERIAL AND METHODS: Specimens of PEEK, polyacetal, and Teflon O-ring materials were prepared for analysis of roughness, surface hardness, and compressive strength. For the fatigue resistance test, new specimens based on acrylic resin were subjected to 2880 insertion and removal cycles. Compression and roughness data were compared with the Kruskal-Wallis and Dunn post hoc test; hardness data with ANOVA and t test; and fatigue and stereomicroscopy with ANOVA with repeated measures, t test, and Bonferroni adjustment (α=.05). RESULTS: Polyacetal had the lowest surface roughness (P=.038). There was a significant difference in hardness among the materials (P<.05). PEEK presented the highest compressive strength among the materials (P<.001). For the fatigue resistance, only polyacetal showed a difference between the initial time-point (P<.05) and subsequent time-points. Polyacetal had a higher fatigue resistance than Teflon (P<.001) and PEEK (P<.05). Regarding the internal deformation of the attachments, a significant difference was observed among the materials (P<.05). CONCLUSIONS: PEEK showed promising results regarding the physicomechanical properties necessary for use as an overdenture attachment when compared with other evaluated materials. In addition, the PEEK attachment showed results comparable to those in the control group (O-rings) in terms of retention.

Stress Distribution Analysis of Novel Dental Mini-Implant Designs to Support Overdenture Prosthesis.

This study aimed to test and compare 2 novel dental mini-implant designs to support overdentures with a commercial model, regarding the stress distribution, by photoelastic analysis. Three different mini-implant designs (Ø 2.0 mm × 10 mm) were tested: G1-experimental threaded (design with threads and 3 longitudinal and equidistant self-cutting chamfers), G2-experimental helical (design with 2 long self-cutting chamfers in the helical arrangement), and G3-Intra-Lock System. After including the mini-implants in a photoelastic resin, they were subjected to a static load of 100 N under two situations: axial and inclined model (30°). The fringe orders (n), that represents the intensity of stresses were analyzed around the mini-implants body and quantified using Tardy's method that calculates the maximum shear stress (τ) value in each point selected. In axial models, less stress was observed in the cervical third mini-implants, mainly in G1 and G2. In inclined models (30°), higher stresses were generated on the opposite side of the load application, mainly in the cervical third of G2 and G3. All mini-implant models presented lower tensions in the cervical third compared with the middle and apical third. The new mini-implants tested (G1 and G2) showed lower stresses than the G3 in the cervical third under axial load, while loading in the inclined model generated greater stresses in the cervical of G2.

Comparison of oral microbiome profile of polymers modified with silver and vanadium base nanomaterial by next-generation sequencing.

This study aimed to evaluate two methods of the incorporation of nanostructured silver vanadate decorated with silver nanoparticles (AgVO3) into acrylic resin and characterize the profile of early and late microbial communities in class and family taxonomic level by pyrosequencing. The specimens were made by adding different concentrations of AgVO3 (1, 2.5, and 5%) to the heat-activated acrylic resin by two methods: vacuum spatulation (VS) and polymeric film (PF). A control group (0%) without AgVO3 was also obtained for both methods. After 24 h and 7 days of incubation in human saliva, biofilm samples were collected, DNA was extracted, and 16S rRNA genes were sequenced by the 454-Roche sequencing platform. Seventeen classes and 51 families of bacteria were identified. The abundance of Bacteroidia, Bacilli, Negativicutes, Fusobacteria and Betaproteobacteria classes decreased after 7 days of incubation, and Clostridia, Gammaproteobacteria, and unclassified bacteria increased. The Negativicutes and Betaproteobacteria classes were more abundant when the PF method was used, and Gammaproteobacteria was more abundant when VS was used. The incorporation of 5% AgVO3 promoted a reduction in the prevalence of Bacilli, Clostridia, Negativicutes, Betaproteobacteria, and unclassified bacteria, and increased Gammaproteobacteria. The addition of AgVO3 to acrylic resin altered the early and mature microbiome formed on the specimen surface, and the PF method presented a more favorable microbial profile than the VS method.

Cytotoxicity and release ions of endodontic sealers incorporated with a silver and vanadium base nanomaterial.

The modification of endodontic sealers with nanoparticles to confer antimicrobial activity allow greater effect, with interaction at a molecular level. The nanostructured silver vanadate decorated with silver nanoparticles (AgVO3) is a nanomaterial unprecedented in dentistry for this application. This study incorporated the AgVO3 into three endodontic sealers of different compositions and evaluate the cytotoxicity and release of compounds. The groups of commercially available AH Plus, Sealer 26, and Endomethasone N and groups of the same sealers with incorporated AgVO3 (at concentrations 2.5, 5, 10%) were prepared, and extracts of the specimens were obtained for 24 h. The cell viability (cytotoxicity) of human gingival fibroblasts (HGF) was assessed after 24 h, 7 and 14 days. Silver (Ag+) and vanadium (V4+/V5+) ion release was quantified after 24 h by ICP-MS. Data were analyzed by Kruskal-Wallis and Dunn's post-hoc (α = 0.05). The cell viability was inversely proportional to treatment time. The Sealer 26 and Endomethasone N groups were cytotoxic for HGF cells, regardless of the incorporation of the AgVO3 (p > 0.05), and the incorporation reduced cell viability of AH Plus (p < 0.05). The release of ions was proportional to the concentration of AgVO3. AH Plus released more Ag+ ions, and Sealer 26 and Endomethasone N releases more V4+/V5+ ions. In conclusion, it was not possible to confirm the influence of AgVO3 on HGF cell viability to Sealer 26 and Endomethasone N, however, nanomaterial influenced cell-viability to AH Plus, so the commercial sealers can be cytotoxic in synergy with the nanomaterial. The release of Ag+ and V4+/V5+ was proportional to the AgVO3 incorporated.

Effect of nanomaterial incorporation on the mechanical and microbiological properties of dental porcelain.

STATEMENT OF PROBLEM: Dental porcelain restorations are subject to biological failures related to secondary caries and periodontal disease leading to prosthesis replacement. PURPOSE: The purpose of this in vitro study was to explore the microbiological and mechanical properties of dental porcelain incorporated with different percentages of silver vanadate (ß-AgVO3) through microbiological analysis, roughness tests, and the Vickers microhardness test. MATERIAL AND METHODS: IPS InLine porcelain specimens were made by using a cylindrical Teflon matrix in the dimensions of 8×2 mm. For the control group, the porcelain was manipulated according to the manufacturer's instructions. The groups incorporating the nanomaterial were prepared with 2.5%, 5%, and 10% of ß-AgVO3, which was added proportionally by mass to the porcelain powder. In vitro microbiologic analysis, roughness tests, and the Vickers microhardness test were performed. RESULTS: Against Streptococcus mutans, the control group showed no inhibition halo (0 mm). All groups with AgVO3 showed a zone of inhibition, the highest for the group with 10% (30 mm) and then the groups with 2.5% (9 mm) and 5% (17 mm). For Vickers microhardness, no statistically significant difference (P<.05) was observed between the evaluated groups. The group with 10% of AgVO3 had the highest mean roughness and was statistically different (P<.001) from the other groups. CONCLUSIONS: Adding ß-AgVO3 to dental porcelain demonstrated antimicrobial effectiveness at all concentrations (2.5%, 5%, and 10%), with no effect on Vickers microhardness. The 10% group had higher roughness than the other groups.

Use of Recorded Poetic Audios to Manage Levels of Anxiety and Sleep Disorders.

Anxiety and sleep disorders are highly prevalent affecting millions of people worldwide. Complementary therapies like art therapy, bibliotherapy, and music have been used to manage these problems. The objective of this study was to evaluate levels of anxiety and sleep disorder symptoms of participants before and after a 30-day intervention of listening to short poetic audios. Thirty-one participants listened to audio-taped messages, which associate music and literature for 30 days. Questionnaires were answered by participants prior to the start of the study (baseline collection) and after 30 days (final collection). Setting: the study was done in the city of Ribeirao Preto (Sao Paulo-Brazil). Participants were 31 adults with anxiety symptoms and sleep problems. The scores of the questionnaires were analyzed and compared by the t test for paired samples (α = 0.05), used to evaluate possible differences in anxiety and stress symptoms and sleep disturbances before and after the 30 days of intervention. Significantly lower levels of anxiety and sleep disorders were found after the 30-day intervention. This study reinforces the benefits of music and literature for improving the quality of life of people by reducing levels of anxiety and sleep disorder symptoms.

Surface treatment of implant materials with antimicrobial nanoparticulates.

This study analyzed nanostructured silver vanadate (AgVO3) films on certain substrate surfaces found in dental implant devices in order to see if these films promote antimicrobial activity. Enamel and glaze vehicles were used to apply the nanomaterial (0.0% [control], 2.5%, 5.0%, and 10.0% concentrations) on 3 substrates (280 specimens per substrate): polytetrafluoroethylene (PTFE), polyacetal, and acrylic resin. An agar diffusion method was performed in triplicate to determine the antimicrobial activity against 8 microorganisms. Physical behavior and morphologic properties were also analyzed. The growth of Pseudomonas aeruginosa, Escherichia coli, Streptococcus mutans, Enterococcus faecalis, and Staphylococcus aureus was inhibited with the application of AgVO3. For Candida albicans, Porphyromonas gingivalis, and Prevotella intermedia, no antimicrobial activity was observed. Except for S mutans, the type of substrate did not influence the microbiologic results. The glaze showed greater antimicrobial efficacy against S aureus, E faecalis, and S mutans than did the enamel vehicle (P < 0.05). Films with 10% AgVO3 showed greater antimicrobial activity (P < 0.05). The choice of substrate or vehicle did not influence the surface roughness (P > 0.05); however, the application of 10% AgVO3 caused greater surface change (P < 0.05). The application of the nanomaterial did not influence the surface hardness of substrates when compared with the control group (P > 0.05). The use of the glaze as a vehicle provided higher hardness values (P < 0.05).

Elemental ion release and cytotoxicity of antimicrobial acrylic resins incorporated with nanomaterial.

OBJECTIVES: This study evaluated the release of ions and the cytotoxicity of acrylic resins incorporated with silver vanadate decorated with silver nanoparticles (AgVO3 ). BACKGROUND: The inhibition of the accumulation of microorganisms on the resins is critical in preventing diseases. However, the hypothesis is that the release of ions from the incorporation of AgVO3 may be important in biocompatibility. MATERIALS AND METHODS: Specimens of autopolymerising (AP) and heat-polymerising resin (HP) with AgVO3 were prepared and immersed in culture medium. The release of silver ions (Ag) and vanadium (V) was evaluated by mass spectrometry with inductively coupled plasma (ICP-MS) (n=9) and the cell viability of fibroblasts L929 by MTT (3-[4,5-dimethylthiazol- 2yl]-2,5-diphenyltetrazolium bromide) (n=12). The results were evaluated with analysis of variance (ANOVA), Tukey and Pearson correlation test (α=.05). RESULTS: The groups containing AgVO3 presented a difference in relation to the control (0%) regarding the release of Ag and V (P<.0001). All groups showed a reduction in L929 viability when compared with the cellular control (100%) (P<.0001). In comparison with the control resins for HP, a reduction in the metabolism of cells occurred starting at 2.5% and for AP at 5% (P<.0001). A positive correlation was found between the concentration of AgVO3 and the ion release, and a negative between the ion release and the cell viability. CONCLUSIONS: Significant numbers of Ag and V ions were released from resins with higher concentrations of AgVO3 , presenting cytotoxicity for cells, suggesting that the use of low concentrations is indicated to avoid risks to patients.

Mechanical properties of silorane-based and methacrylate-based composite resins after artificial aging.

The aim of this study was to compare the compressive strength of a silorane-based composite resin (Filtek P90) to that of conventional composite resins (Charisma, Filtek Z250, Fill Magic, and NT Premium) before and after accelerated artificial aging (AAA). For each composite resin, 16 cylindrical specimens were prepared and divided into 2 groups. One group underwent analysis of compressive strength in a universal testing machine 24 hours after preparation, and the other was subjected first to 192 hours of AAA and then the compressive strength test. Data were analyzed by analysis of variance, followed by the Tukey HSD post hoc test (α = 0.05). Some statistically significant differences in compressive strength were found among the commercial brands (P < 0.001). The conventional composite resin Fill Magic presented the best performance before (P < 0.05) and after AAA (P < 0.05). Values for compressive strength of the silorane-based composite were among the lowest obtained, both before and after aging. Comparison of each material before and after AAA revealed that the aging process did not influence the compressive strength of the tested resins (P = 0.785).

Esthetic solution to malpositioned implants with remodeling of soft tissue: a case report.

This article describes a clinical case of gingival conditioning with provisional fixed prostheses to improve the esthetics of the soft tissues adjacent to fixed prostheses placed on malpositioned implants. Gradual application of pressure to the tissues is an easy, nontraumatic technique for inducing formation of papillae and reestablishing the appropriate shape and contour of the gingival tissues, thereby improving esthetics and phonetics. The proposed treatment proved to be effective in remodeling the surrounding soft tissues, providing suitable contours, and restoring esthetics and function lost due to surgical treatment with malpositioned implants.

Analysis of the influence of implant shape on primary stability using the correlation of multiple methods.

OBJECTIVES: The purpose of this study was to analyze the influence of the shape of various implants and the density of substrate on primary stability using a combination of methods. MATERIALS AND METHODS: Fifty-four Neodent® brand cylindrical and conical implants with different prosthetic platforms were used. Implants were inserted into a pork rib bone and polyurethane blocks. Primary stability was assessed by insertion torque (IT), resonance frequency analysis (RFA), and pullout strength. Screws were also analyzed by scanning electron microscopy (SEM) before insertion and after removal to justify their use for inserting in different substrates. RESULTS: The conical cone morse implant had the highest average for all of the assays performed and was significantly different (p < 0.05) from the cylindrical implants for IT in the bone, pullout strength in the 40 per cubic foot (PCF) polyurethane, and the bone. The internal hex cylindrical implant had the lowest averages, which were significantly different (p < 0.05) from the conical implants for IT and RFA in the bone, pullout strength in the 40 PCF polyurethane, and the bone. The IT, RFA, and pullout strength assays were moderately correlated, and the photomicrographs did not reveal changes in the implants. CONCLUSIONS: The analysis of different implants showed a better primary stability of tapered implants; the density of the substrate influences the primary stability and the 15 PCF polyurethane was not adequate to evaluate primary stability; correlation was obtained between the different methodologies of analysis of primary stability. CLINICAL RELEVANCE: The study shows the influence of different implant macro-geometries and densities of substrates on primary stability.