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1.
Risk Anal ; 43(6): 1097-1114, 2023 06.
Artículo en Inglés | MEDLINE | ID: mdl-35853833

RESUMEN

Foodborne disease caused by nontyphoidal Salmonella (NTS) is one of the most important food safety issues worldwide. The objectives of this study were to carry out microbial monitoring on the prevalence of NTS in commercial ground pork, investigate consumption patterns, and conduct a quantitative microbiological risk assessment (QMRA) that considers cross-contamination to determine the risk caused by consuming ground pork and ready-to-eat food contaminated during food handling in the kitchen in Chengdu, China. The food pathway of ground pork was simplified and assumed to be several units according to the actual situation and our survey data, which were collected from our research or references and substituted into the QMRA model for simulation. The results showed that the prevalence of NTS in ground pork purchased in Chengdu was 69.64% (95% confidence interval [CI], 60.2-78.0), with a mean contamination level of -0.164 log CFU/g. After general cooking, NTS in ground pork could be eliminated (contamination level of zero). The estimated probability of causing salmonellosis per day was 9.43E-06 (95% CI: 8.82E-06-1.00E-05), while the estimated salmonellosis cases per million people per year were 3442 (95% CI: 3218-3666). According to the sensitivity analysis, the occurrence of cross-contamination was the most important factor affecting the probability of salmonellosis. To reduce the risk of salmonellosis caused by NTS through ground pork consumption, reasonable hygiene prevention and control measures should be adopted during food preparation to reduce cross-contamination. This study provides valuable information for household cooking and food safety management in China.


Asunto(s)
Carne de Cerdo , Carne Roja , Intoxicación Alimentaria por Salmonella , Infecciones por Salmonella , Animales , Humanos , Porcinos , Carne Roja/microbiología , Carne de Cerdo/análisis , Microbiología de Alimentos , Salmonella , Intoxicación Alimentaria por Salmonella/epidemiología , Intoxicación Alimentaria por Salmonella/prevención & control , Infecciones por Salmonella/epidemiología , Medición de Riesgo/métodos , Manipulación de Alimentos/métodos , Contaminación de Alimentos/análisis
2.
Foodborne Pathog Dis ; 18(3): 219-227, 2021 03.
Artículo en Inglés | MEDLINE | ID: mdl-33471597

RESUMEN

One objective of this study was to determine overall prevalence of Salmonella in ground pork from U.S. retail stores over three seasons including both case-ready and store-ground packages. Package types collected included: overwrap, chub, modified atmosphere packaging, and other (plastic or wax paper wrapped). Because package type represents different production systems and are subject to varied microbiological government regulation and testing methodologies, both USDA-FSIS and FDA Salmonella isolation protocols were performed. Another objective of the study was to determine serotypes and antimicrobial susceptibility profiles of the isolates obtained from the ground pork samples. Ground pork aliquots were subjected to real-time PCR. Recovered isolates were serotyped and minimum inhibitory concentration analysis to 15 antimicrobials was determined using microbroth dilution. Overall prevalence of Salmonella in ground pork from the 865 samples collected was 1.39%. Prevalence was not affected by package type (p = 0.29) nor grind location (case-ready vs. store-ground; p = 0.17). Season affected Salmonella prevalence (p = 0.05) with most isolates found during fall, and there was a tendency for geographic region to affect prevalence (p = 0.07). The USDA Salmonella isolation method was more effective at recovering isolates (p = 0.01) compared with the FDA methodology and yielded a kappa statistic of 0.26 as a measure of agreement. The serotypes isolated included: Infantis, 4,5,12:i:-, Brandenburg, Typhimurium var 5-, Seftenberg, and Johannesburg with only two packages containing multiple serotypes. No isolates were resistant to antibiotics commonly used to treat human Salmonella infections including extended spectrum cephalosporins or fluoroquinolones. Although the recovery of Salmonella from retail ground pork samples was rare, Salmonella Typhimurium (and its monophasic variant 4,5,12:i:-), which are among the most common serovars recovered from human infections, were recovered. Therefore, more effective strategies to further reduce or eliminate these pathogens from retail pork products are warranted.


Asunto(s)
Farmacorresistencia Bacteriana Múltiple , Microbiología de Alimentos/estadística & datos numéricos , Carne de Cerdo/microbiología , Salmonella/aislamiento & purificación , Animales , Humanos , Pruebas de Sensibilidad Microbiana , Prevalencia , Serogrupo , Porcinos , Estados Unidos/epidemiología
3.
Risk Anal ; 39(10): 2237-2258, 2019 10.
Artículo en Inglés | MEDLINE | ID: mdl-31039285

RESUMEN

The inclusion of deep tissue lymph nodes (DTLNs) or nonvisceral lymph nodes contaminated with Salmonella in wholesale fresh ground pork (WFGP) production may pose risks to public health. To assess the relative contribution of DTLNs to human salmonellosis occurrence associated with ground pork consumption and to investigate potential critical control points in the slaughter-to-table continuum for the control of human salmonellosis in the United States, a quantitative microbial risk assessment (QMRA) model was established. The model predicted an average of 45 cases of salmonellosis (95% CI = [19, 71]) per 100,000 Americans annually due to WFGP consumption. Sensitivity analysis of all stochastic input variables showed that cooking temperature was the most influential parameter for reducing salmonellosis cases associated with WFGP meals, followed by storage temperature and Salmonella concentration on contaminated carcass surface before fabrication. The input variables were grouped to represent three main factors along the slaughter-to-table chain influencing Salmonella doses ingested via WFGP meals: DTLN-related factors, factors at processing other than DTLNs, and consumer-related factors. The evaluation of the impact of each group of factors by second-order Monte Carlo simulation showed that DTLN-related factors had the lowest impact on the risk estimate among the three groups of factors. These findings indicate that interventions to reduce Salmonella contamination in DTLNs or to remove DTLNs from WFGP products may be less critical for reducing human infections attributable to ground pork than improving consumers' cooking habits or interventions of carcass decontamination at processing.


Asunto(s)
Ganglios Linfáticos/microbiología , Carne Roja/microbiología , Intoxicación Alimentaria por Salmonella/epidemiología , Infecciones por Salmonella/epidemiología , Salmonella/aislamiento & purificación , Animales , Humanos , Medición de Riesgo , Intoxicación Alimentaria por Salmonella/prevención & control , Porcinos
4.
J Sci Food Agric ; 99(6): 2735-2742, 2019 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-30350316

RESUMEN

BACKGROUND: Kimchi is a Korean, traditional fermented food made from Korean cabbage, radish, fermented jeotgal, ginger, garlic, and red pepper powder. It is a good source of natural antioxidants such as phenolic compounds, flavonoids, vitamins, and carotenoids. In this study, the antioxidant effects of various kimchi extracts on raw ground pork during refrigerated storage were investigated. Raw ground pork samples were treated with ascorbic acid, butylated hydroxyl toluene, baechu kimchi extract (BKE), gat kimchi extract (GKE), puchu kimchi extract (PKE), and white kimchi extract (WKE) and compared with raw ground pork without antioxidant treatment (NC). RESULTS: Increased metmyoglobin (MetMb), thiobarbituric acid reacting substance (TBARS), and total bacterial counts (TBC) were observed in all meat samples after storage, whereas pH, lightness, and redness values tended to decrease with increased storage time. All treated samples had lower TBARS and MetMb values and TBC compared to the control samples. Various kimchi ethanol extracts protected raw ground pork from lipid oxidation. The most potent antioxidant was GKE, whereas WKE was the weakest. CONCLUSIONS: This study suggests that the tested extracts, especially kimchi, have potential as natural preservatives to reduce colour degradation, lipid oxidation, and bacterial count in raw ground pork meat. © 2018 Society of Chemical Industry.


Asunto(s)
Alimentos Fermentados , Almacenamiento de Alimentos , Peroxidación de Lípido , Carne/análisis , Extractos Vegetales/química , Animales , Frío , Refrigeración , Porcinos
5.
Asian-Australas J Anim Sci ; 32(2): 265-273, 2019 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-30056664

RESUMEN

OBJECTIVE: Terminalia arjuna plant, specially its leaves, bark, and roots, are widely used in traditional herbal medicine due to presence of bioactive components and being a rich source of natural antioxidants. But its fruit has not been used for any such purposes despite its potential to retard oxidation. Hence, the antioxidant potential of Arjuna fruit extract (AFE) in retarding lipid and protein oxidation of raw ground pork was evaluated during refrigerated storage for 9 days. METHODS: The AFEs were prepared using different solvents viz. ethanol (EH), water, ethanol: water (60:40) and methanol:hot water (60:40). The AFEs were analysed for total phenolic content (TPC), 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity and reducing power. Water extract (WE) and ethanol-water extract (EH-WE) were selected and incorporated at 1.0% into freshly minced pork meat and compared with a synthetic antioxidant, in retarding lipid and protein oxidation during storage. RESULTS: The TPC in AFEs using different solvents ranged from 11.04 to 16.53 mg gallic acid equivalents/g and extracts exhibited appreciable scavenging activity ranging from 50.02% to 58.62%. Arjuna extracts significantly (p<0.05) improved the colour score of meat samples by reducing the formation of metmyoglobin during storage. Both the AFEs (WE and EH-WE) significantly (p<0.05) lowered the thiobarbituric acid reactive substances value, peroxide formation and formation of protein carbonyls in raw pork than control sample during storage. Upon sensory evaluation of all samples, it was found that AFE treatment could prolong the storage period of meat samples, without influencing the colour and odour score, up to 6 days. CONCLUSION: AFEs used at 1% improved the oxidative stability, colour and odour score and prolonged the refrigerated shelf life of ground pork up 6 days. Therefore, AFE could be explored as an alternative natural antioxidant in retarding lipid and protein oxidation in meat products.

6.
Sensors (Basel) ; 17(4)2017 Apr 02.
Artículo en Inglés | MEDLINE | ID: mdl-28368342

RESUMEN

Escherichia coli O157:H7 is known to cause serious diseases including hemorrhagic colitis and hemolytic uremic syndrome. A gold nanoparticle lateral flow immunoassay (Au-LFIA) was used to detect Escherichia coli O157:H7 in ground pork samples. False-positive results were detected using Au-LFIA; a Citrobacterfreundii strain was isolated from the ground pork samples and identified by using CHROmagarTM plates, API 20E, and 16S RNA sequencing. Since C.freundii showed cross-reactivity with E. coli O157:H7 when Au-LFIA test strips were used, a novel method combining modified enrichment with a lateral flow immunoassay for accurate and convenient detection of E. coli O157:H7 in ground pork was developed in this study to minimize these false positives. MacConkey broth was optimized for E. coli O157:H7 enrichment and C.freundii inhibition by the addition of 5 mg/L potassium tellurite and 0.10 mg/L cefixime. Using the proposed modified enrichment procedure, the false-positive rate of ground pork samples spiked with 100 CFU/g C.freundii decreased to 5%.


Asunto(s)
Escherichia coli O157 , Animales , Recuento de Colonia Microbiana , Microbiología de Alimentos , Oro , Inmunoensayo , Nanopartículas del Metal , Carne Roja , Porcinos
7.
J Environ Sci Health B ; 51(9): 622-7, 2016 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-27192496

RESUMEN

The purpose of this study was to characterize the microbial community in ground pork using molecular approaches. Forty six ground pork products were purchased from local stores in the north central area of South Korea. Aerobic plate counts varied 4.23 ± 5.14 × 10(5) CFU/g with the range between 5.00 × 10(3) and 1.85 × 10(6) CFU/g for ground pork samples. Four ground meat samples were further processed for metagenomic analysis. Pseudomonas species was the most relative abundant with a wide range occurring (1.72 to 77.7%) as part of the microbial genera in ground pork. Bacteria such as Carnobacterium, Yersinia, Photobacterium were also identified in ground pork. Despite the prominence of certain genera across all samples there was still extensive microbial diversity among ground pork products that originated from different slaughter houses and were processed in different markets. Such diversity indicates that designing interventions to extend shelf life may be hampered by the extensive variability in the microbial consortia associated with pork products. However, this diversity may be useful for developing microbial traceability signatures unique to a slaughter house or a particular market.


Asunto(s)
Microbiología de Alimentos , Microbiota , Carne Roja/microbiología , Animales , Servicios de Alimentación , Metagenómica , República de Corea , Porcinos
8.
J Sci Food Agric ; 95(14): 2832-7, 2015 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-25428271

RESUMEN

BACKGROUND: Plant proteins and polysaccharides are often utilised in ground meat products as meat binders, gelling agents, texture stabilisers or fat substitutes. The aim of this study was to investigate the effects of the incorporation of 57 g kg(-1) soy protein isolate (SPI), 7 g kg(-1) carrageenan (CAR) and SPI/CAR mixture on the quality of ground pork patties. RESULTS: Ground pork patties with individual SPI and CAR or SPI/CAR mixture showed either retained or improved emulsion stability, physicochemical properties and dynamic rheology compared with control samples. Although there were no significant colour differences among treatments, samples with SPI/CAR mixture presented higher texture profile values for hardness and chewiness compared with other treatments (P < 0.05). Patties with additives showed significantly lower cooking loss and better thermal emulsion stability than control samples owing to a lower release rate of water and fat (P < 0.05). Compared with control samples or those with individual SPI and CAR, patties with SPI/CAR mixture formed a smoother and more continuous structure with a more compact protein matrix. CONCLUSION: The results indicate that a mixture of SPI and CAR can be effectively used as a functional ingredient to improve the quality of ground pork patties.


Asunto(s)
Carragenina , Emulsiones/química , Aditivos Alimentarios , Manipulación de Alimentos , Productos de la Carne/análisis , Carne Roja , Proteínas de Soja , Animales , Color , Culinaria , Humanos , Reología , Porcinos , Temperatura
9.
Food Sci Anim Resour ; 44(2): 408-429, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38764518

RESUMEN

The color, lipid oxidation, heme iron (HI) and non-heme iron (NHI) contents, metmyoglobin content and Soret band of myoglobin of ground pork subjected to supercritical CO2 treatment under different conditions, or to heat treatment (40°C, 2 h) and subsequent storage at 4°C were evaluated during 9-day period. Supercritical CO2 treatment significantly increased CIE L* and CIE b* values of ground pork during subsequent storage, while the HI content was slightly affected. In general, CIE a* value and metmyoglobin content were decreased. Supercritical CO2 treatment for 2 h could increase the thiobarbituric acid-reactive substances (TBARS) value, while treatment for 1 h or less had no effect. The NHI content could be increased only after treatment at above 40°C or 17.2 MPa for 2 h. The Soret band of myoglobin was shifted to longer wavelength. Increasing treatment temperature from 35°C to 45°C could increase CIE L*, CIE a*, CIE b* and TBARS values, HI and NHI contents of the ground pork, while decreasing metmyoglobin content. As the treatment pressure increased from 13.8 MPa to 20.7 MPa, CIE b* and TBARS values were decreased, while the NHI and metmyoglobin contents were increased. However, the other parameters were unchanged. Extending exposure time from 0.5 h to 2 h could increase CIE L*, CIE b* and TBARS values, HI contents, while decreasing CIE a* value and metmyoglobin content. Correlation analysis showed that the TBARS value was significantly and negatively correlated with the HI content or metmyoglobin content in samples treated at 40°C or above for 2 h.

10.
Food Res Int ; 173(Pt 1): 113231, 2023 11.
Artículo en Inglés | MEDLINE | ID: mdl-37803544

RESUMEN

The main purpose of this research was to investigate the effect of power level of 915 MHz microwave heating on the inactivation of foodborne pathogens in ground pork and its bactericidal mechanism. It was demonstrated that the heating rate was proportional to the power level. For instance, the heating rates observed at microwave heating powers of 2, 3, 4, and 5 kW were 1.70, 2.77, 3.35, and 4.03℃/s, respectively. The bactericidal effect of microwave heating also significantly (P < 0.05) increased with increasing power level. In particular, when ground pork was subjected to microwave heating at 5 kW, the reduction level of pathogens was>2 log units higher than at 2 kW. To determine the bactericidal mechanism of microwave heating depending on power level, changes in transmembrane potential and DNA damage were determined using fluorescence. The extent of depolarization in membrane potential of pathogens significantly (P < 0.05) increased as power level increased. There was no significant difference in degree of DNA damage at different power levels. However, the percentage of DNA damage was>86% at all power levels. The transmembrane potential assay indicates that the bacteria exhibited dramatic pore formation on the membrane at 5 kW. Through transmission electron microscopy, the electroporation effect was enhanced as power level increased. Moreover, the quality of ground pork subjected to microwave heating at 2-5 kW was determined by measuring the moisture content, cooking loss, and texture profile.


Asunto(s)
Carne de Cerdo , Carne Roja , Animales , Porcinos , Microondas , Calefacción , Calor
11.
Foods ; 12(16)2023 Aug 21.
Artículo en Inglés | MEDLINE | ID: mdl-37628134

RESUMEN

Whey protein hydrolysate (WPH) has made a breakthrough in inhibiting oxidative deterioration and improving the quality of meat products during storage. Based on our previous study of extracting the most antioxidant active fraction I (FI, the molecular weight < 1 kDa) from whey protein hydrolysates of different molecular weights, the present study continued to delve into the effects of WPH with fraction I on the structure and function of myofibrillar proteins (MP) in ground pork during the freeze-thaw (F-T) cycles. With the number of F-T cycles raised, the total sulfhydryl content, the relative contents of α-helix, Ca2+-ATPase activity, K+-ATPase activity, solubility, emulsion activity index (EAI), and emulsion stability index (ESI) of MP gradually decreased. Conversely, the carbonyl content and the relative content of random curl showed an increasing trend. In particular, the damage to the structure and the function of MP became more pronounced after three F-T cycles. But, during F-T cycles, FI stabilized the structure of MP. Compared to the control group, the 10% FI group showed a remarkable improvement (p < 0.05) in the total sulfhydryl content, Ca2+-ATPase activity, K+-ATPase activity, solubility, EAI and ESI after multiple F-T cycles, suggesting that 10% FI could effectively inhibit protein oxidation and had the influence of preserving MP function properties. In conclusion, WPH with fraction I can be used as a potential natural antioxidant peptide for maintaining the quality of frozen processed meat products.

12.
Heliyon ; 9(3): e14486, 2023 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-36950632

RESUMEN

The study evaluated the bioactive components and antioxidant properties of sugarcane phenolic extracts (SCPE) against lipid oxidation in raw ground pork and beef during refrigerated storage conditions. Results showed that SCPE has a high total phenolic content of 35.9 g GAE/100 g sample majority of which are vanillic (799.77 mg/100 g), sinapic (434.38 mg/100 g), and coumaric (342.37 mg/100 g) acids. These phenolic compounds exhibited strong radical scavenging activities against DPPH (191.00 mg TE/g) and ABTS (359.80 mg TE/g) radical, ferric reducing capacity (97.80 mg TE/g), and lipid peroxidation inhibition (120.20 mg TE/g) activities which are comparable to BHT. Supplementation of SCPE at 400 mg/kg fresh weight of ground pork and ground beef improved the antioxidant activities of meat samples, leading to the delay in TBARS formation, lower reduction in heme iron content, and minimal increase in metmyoglobin content during 14-day refrigerated storage as compared to BHT-treated and control. Moreover, the redness of meat was preserved in SCPE- and BHT-treated samples as measured by a* color value. Correlation heat maps showed that TBARS, metmyoglobin, and ΔE are positively correlated with each other, and at the same time showed negatively correlated with heme iron and a*. This study revealed that antioxidant extracts from sugarcane presented a positive effect on the oxidative stability of raw ground pork and beef during refrigerated storage.

13.
Int J Biol Macromol ; 172: 464-474, 2021 Mar 01.
Artículo en Inglés | MEDLINE | ID: mdl-33465361

RESUMEN

Porous starch (PS) was used as the core material carrier to adsorb fennel essential oil (FEO). Using sodium alginate (SA)-chitosan (CS) as the wall material and glutaraldehyde as the curing cross-linking agent, CS/SA/PS-FEO microcapsules were successfully prepared by polyelectrolyte complex coagulation method. The formation process, structural properties and release behavior of CS/SA/PS-FEO microcapsules were analyzed. The results showed that the essential oil was encapsulated in the form of micro-capsules according to infrared spectroscopy and X-ray diffraction analysis. In open and closed systems, the 16-day cumulative release rate of FEO obtained was 70.62% and 43.87%, respectively indicating that the prepared CS/SA/PS-FEO microcapsules had a good sustained-release ability. The fennel essential oil micro-capsules exhibited good antibacterial and antioxidant activities, delayed the oxidation of fat and protein, reduced the total viable counts, total volatile-base nitrogen and methemoglobin. The textural property and status of water (analyzed by NMR) suggested that the quality of the meat can be maintained for an extended period by incorporating the CS/SA/PS-FEO microcapsules in the minced pork meat.


Asunto(s)
Antibacterianos/farmacología , Antioxidantes/farmacología , Foeniculum/química , Almacenamiento de Alimentos/métodos , Productos de la Carne/análisis , Aceites Volátiles/farmacología , Almidón/química , Alginatos/química , Animales , Antibacterianos/aislamiento & purificación , Antioxidantes/aislamiento & purificación , Cápsulas , Quitosano/química , Composición de Medicamentos/métodos , Humanos , Concentración de Iones de Hidrógeno , Metahemoglobina/análisis , Aceites Volátiles/aislamiento & purificación , Carne de Cerdo/análisis , Porosidad , Porcinos
14.
Spectrochim Acta A Mol Biomol Spectrosc ; 249: 119307, 2021 Mar 15.
Artículo en Inglés | MEDLINE | ID: mdl-33348095

RESUMEN

Hyperspectral imaging (HSI) technique was investigated to explore a feasible protocol for detecting the potential offal (lung) adulteration in ground pork. Tested samples (176 adulterated and 2 controls) were first prepared with adulterant of ground lung in range of 0%-100% (w/w) at 10% intervals. After hyperspectral images were acquired and calibrated in reflectance mode (400-1000 nm), full spectra were extracted from identified regions of interests (ROIs) and then transformed into absorbance and Kubelka-Munck spectral units, respectively. Partial least squares regression (PLSR) models based on full spectra showed that raw reflectance spectra with no preprocessings performed best with coefficient of determination (Rp2) of 0.98, root mean square error (RMSEP) of 4.25%, and ratio performance deviation (RPD) of 7.53 in prediction set. To reduce the high dimensionality of spectra, data was further explored using principal component loadings, two-dimensional correlation spectroscopy (2D-COS), and regression coefficients (RC), respectively. The optimal performance of established simplified PLSR model were acquired using eleven featured wavelengths selected by PC loadings with Rp2 of 0.98, RMSEP of 4.47% and RPD of 7.16. Finally, the limit of detection (LOD) was calculated to be a satisfactory 7.58%, and readily discernible visualization procedure using preferred simplified PLSR model yielded satisfactory spatial distribution of adulteration situation. Control samples with known distribution were also visualized to successfully prove the validity. Consequently, this research offers an alternative assay for visually and rapidly detecting offal of lung adulteration in ground pork.


Asunto(s)
Carne de Cerdo , Carne Roja , Animales , Imágenes Hiperespectrales , Análisis de los Mínimos Cuadrados , Carne/análisis , Carne Roja/análisis , Espectroscopía Infrarroja Corta , Porcinos
15.
J Food Prot ; 82(11): 1844-1850, 2019 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-31599649

RESUMEN

A total of 514 raw pork samples (395 ground or nonintact and 119 intact samples) were purchased at retail stores in Pennsylvania, Delaware, and New Jersey between July and December 2017. All raw pork samples were screened for serogroup O26, O45, O103, O111, O121, O145, or O157:H7 cells of Shiga toxin-producing Escherichia coli (STEC-7) using standard microbiological and molecular methods. In short, 21 (5.3%) of the 395 ground or nonintact pork samples and 3 (3.4%) of the 119 intact pork samples tested positive via the BAX system real-time PCR assay for the stx and eae virulence genes and for the somatic O antigens for at least one of the STEC-7 serogroups. However, none of these 24 presumptive-positive pork samples subsequently yielded a viable isolate of STEC displaying a STEC-7 serogroup-specific surface antigen in combination with the stx and eae genes. These data suggest that cells of STEC serogroups O26, O45, O103, O111, O121, O145, or O157:H7 are not common in retail raw pork samples in the mid-Atlantic region of the United States.


Asunto(s)
Microbiología de Alimentos , Carne de Cerdo , Serogrupo , Escherichia coli Shiga-Toxigénica , Animales , Mid-Atlantic Region , Carne de Cerdo/microbiología , Escherichia coli Shiga-Toxigénica/clasificación , Escherichia coli Shiga-Toxigénica/aislamiento & purificación
16.
Korean J Food Sci Anim Resour ; 38(6): 1179-1188, 2018 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-30675110

RESUMEN

This study aimed to investigate the combined effect of using natural calcium mixtures and various binding ingredients as replacers for synthetic phosphate in ground pork products. We performed seven treatments: control (0.3% phosphate blend), treatment 1 (0.5% natural calcium mixtures [NCM, which comprised 0.2% oyster shell calcium and 0.3% egg shell calcium powder] and 0.25% egg white powder), treatment 2 (0.5% NCM and 0.25% whey protein concentrate), treatment 3 (0.5% NCM and 0.25% concentrated soybean protein), treatment 4 (0.5% NCM and 0.25% isolated soybean protein), treatment 5 (0.5% NCM and 0.25% carrageenan), and treatment 6 (0.5% NCM and 0.25% collagen powder). All the treatment mixtures had higher pH and lower cooking loss than the control, which was treated with phosphate. We found that NCM and binding ingredients had no negative effects on the moisture content, lightness, and yellowness of the cooked ground pork products. Treatments 3 and 4 showed significantly lower CIE a* values than the control. Treatments 2 and 6 improved the textural properties of the products. In conclusion, the combination of NCM with whey protein concentrate or collagen powder could be suitable for producing phosphate-free meat products.

17.
Korean J Food Sci Anim Resour ; 38(6): 1246-1252, 2018 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-30675117

RESUMEN

This study was conducted to investigate the effects of the addition levels of a phosphate replacer blend in ground pork sausages. The phosphate replacer consisted of 0.2% oyster shell calcium powder, 0.3% egg shell calcium powder, and 0.25% whey protein concentrate. Depending on the presence or absence of synthetic phosphate and the addition level of phosphate replacer, the following products were processed: control (+) (0.3% phosphate), control (-) (non-phosphate), 20AL (20% replacer), 40AL (40% replacer), 60AL (60% replacer), 80AL (80% replacer), and 100AL (100% replacer). The pH values of pork sausages increased (p<0.05) with increasing addition level of the phosphate replacer. When more than 40% of the phosphate replacer was added to pork samples (40AL, 60AL, 80AL, and 100AL), cooking loss was significantly reduced compared to both the control (+) and control (-). However, no significant differences were observed in the moisture content and CIE L* values between the controls and the treatments with a phosphate replacer. The control (+) and 100AL treatment had the highest (p<0.05) hardness, but the samples with the phosphate replacer were not significantly different in cohesiveness and springiness from the control (+). As addition level increased, the gumminess and chewiness of the products with the phosphate replacer increased, which were lower than those of the control (+). Therefore, more than 40% of a phosphate replacer may possibly substitute synthetic phosphate to improve product yields in ground pork sausages, although further studies may be needed for improving the textural properties of the final products.

18.
Korean J Food Sci Anim Resour ; 37(3): 456-463, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-28747832

RESUMEN

The aim of this study was to identify the optimal and superior type of natural calcium for replacing phosphate in cooked ground pork products. To achieve this, 0.5% eggshell calcium (ESC), oyster shell calcium (OSC), marine algae calcium (MAC), or milk calcium (MC) was added to ground pork meat products. The effect of this substitution was studied by comparing the substituted products with products containing 0.3% phosphate blend (control). ESC was considered an ideal phosphate replacer for minimizing the cooking loss, which likely resulted from the increase in the pH of the product. Among the other natural calcium types, OSC treatment did not cause a significant increase in pH, but it lowered the cooking loss. CIE L* values were higher (p<0.05) in products treated with OSC or MC than the control, and lowest (p<0.05) in the products with ESC. However, products with ESC had higher (p<0.05) CIE a* and CIE b* values than the control and products treated with other powders. Compared to the control, products treated with ESC and OSC had similar substitution effects on the textural properties of the products. Therefore, the results of this study suggested that the combined use of ESC and OSC could be a potentially effective method for replacing synthetic phosphate in ground pork products.

19.
Meat Sci ; 125: 114-120, 2017 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-27940420

RESUMEN

The effect of combined treatment with supercritical CO2 (2000psi, 35°C for 2h) and rosemary powder (2.5% and 5.0% (w/w)) on microbiological and physicochemical properties of ground pork stored at 4°C was investigated. The changes in total viable count, pH, total volatile base nitrogen (TVB-N), lipid oxidation and instrumental color (CIE L⁎, a⁎, b⁎) were analyzed during a week period of refrigerated storage. It was found that microbial populations were reduced by supercritical CO2 treatment, with the more pronounced effect being achieved by combined treatment with supercritical CO2 and 5.0g rosemary powder/100g meat. Supercritical CO2 treatment for 2h could accelerate lipid oxidation of ground pork during refrigerated storage, whereas combination with rosemary can significantly slow down the increase of oxidation rate. Combined treatment of supercritical CO2 and rosemary significantly increased L⁎ and b⁎ values of the ground pork, while the a⁎, pH and TVB-N value were not affected as compared to the treatment with supercritical CO2 alone. The results of this study indicate that combined treatment of supercritical CO2 and rosemary may be useful in the meat industry to enhance the storage stability of ground pork treated with long time exposure of supercritical CO2 during refrigerated storage.


Asunto(s)
Dióxido de Carbono/análisis , Manipulación de Alimentos , Productos de la Carne/microbiología , Carne Roja/microbiología , Rosmarinus/química , Animales , Frío , Recuento de Colonia Microbiana , Color , Grasas de la Dieta/análisis , Contaminación de Alimentos , Microbiología de Alimentos , Embalaje de Alimentos , Conservación de Alimentos , Almacenamiento de Alimentos , Concentración de Iones de Hidrógeno , Productos de la Carne/análisis , Nitrógeno/análisis , Polvos , Carne Roja/análisis , Porcinos , Sustancias Reactivas al Ácido Tiobarbitúrico/análisis , Compuestos Orgánicos Volátiles/análisis
20.
Meat Sci ; 127: 30-34, 2017 May.
Artículo en Inglés | MEDLINE | ID: mdl-28110127

RESUMEN

This research was conducted to study the effects of bacteriophage application during tumbling on Salmonella populations in ground meat and poultry. Red meat trim and poultry were inoculated with a Salmonella cocktail to result in a contamination level of 7logCFU/g in ground products. A commercial preparation containing bacteriophages S16 and Felix-O1a (FO1a) was applied during tumbling at 107 and 108PFU/ml. Samples were held at 4°C for 6h and 18h (red meat) and 30min and 6h (poultry). Overall, bacteriophage application on trim reduced 1 and 0.8logCFU/g of Salmonella in ground beef and ground pork, respectively. For ground chicken and ground turkey, Salmonella was reduced by 1.1 and 0.9logCFU/g, respectively. This study shows that bacteriophage application during tumbling of red meat trim and poultry can provide additional Salmonella control in ground products.


Asunto(s)
Bacteriófagos , Manipulación de Alimentos/métodos , Microbiología de Alimentos , Inocuidad de los Alimentos/métodos , Carne/microbiología , Salmonella/crecimiento & desarrollo , Animales , Bovinos , Recuento de Colonia Microbiana , Humanos , Productos de la Carne/microbiología , Pavos
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