Assessment of episodes of pneumonia and diarrhea in vaccinated and unvaccinated children under 60 months of age.

OBJECTIVE: To assess the episodes of pneumonia and diarrhea in vaccinated and unvaccinated children under 60 months of age. METHODS: This descriptive cross-sectional study was carried out at Darul Sehat Hospital and SESSI, Karachi in the Department of Pediatrics from 1st November 2018 to 3rd February 2019. An interview based questionnaire was administered and selection of participants was done by convenience sampling. Total of 196 participants were selected for interview. RESULTS: Total 196 participants were interviewed which included mothers of children between the age group of 1- 60 months. The questions were entered on the questionnaire after taking consent from the mothers. Among them, males were 98 (52.7%) and females were 88(47.3%). One hundred seventy two (88.7%) children were vaccinated for pneumococcal and rotavirus whereas unvaccinated children were 22(11.3%). There was an incidence of 66(63.5%) for loose watery diarrhea. In vaccinated children, grading of diarrhea was found to be severe cases as 30 (34.9%), moderate cases as 27 (31.4%) and mild cases as 29(33.7%). For unvaccinated children, severe cases were 12 (66.7%) and for mild and moderate cases were (11.1%) and 04(22.2%) respectively with p value of 0.035. As compared to their unvaccinated counterparts, the frequency of severe pneumonia was far less than cough and cold in vaccinated children (p<0.001). CONCLUSION: There is significant reduction in cases of severe pneumonia in children receiving pneumococcal vaccine as compared to children receiving Rota vaccine there is moderate reduction in cases of severe diarrhea. The overall coverage of Pneumococcal and Rota vaccines was higher in our sample population. Efforts should be made to increase the awareness of Rotavirus vaccination in order to have better coverage in future.

Presence of Enteric Viruses in Shellfish Samples from the Persian Gulf.

Shellfishes are a significant economic and nutritious seafood amongst people in different countries. Seafood products, particularly shellfish, are potential reservoirs of enteric viruses. This research investigated the incidence of rotavirus (RoV), norovirus (NoV) GI and GII, hepatitis A virus (HAV), and hepatitis E virus (HEV) in shellfish samples from the Persian Gulf, Iran. One hundred and fifty shellfish samples were collected. RNA extraction and cDNA synthesis were performed using commercial kits. The real-time polymerase chain reaction assessed the presence of enteric viruses in extracted cDNA samples. Thirty-two out of 150 (21.33%) shellfish samples were contaminated with enteric viruses. Prevalence rates of NoV GI, NoV GII, HAV, and RoV amongst shellfish samples were 8.00%, 11.33%, 1.33%, and 0.66%, respectively. There were no contaminated shellfish samples with HEV. Simultaneous prevalence of HAV and NoV GI, and HAV and NoV GII viruses were 0.66% and 0.66%, respectively. Examined viruses had a higher prevalence in shellfish samples collected in the winter season (P<0.05). Prevalence of HAV, RoV, NoV GI, and NoV GII amongst shellfish samples gathered in the winter season was 2.85%, 9.09%, 11.90%, and 20%, respectively. To the best of our knowledge, this was the first report of the incidence of enteric viruses, particularly HAV, NoV GI, NoV GII, and RoV, in shellfish samples from the Persian Gulf, Iran. Shellfish samples may serve as a potential source of enteric viruses for the human population. Therefore, routine viral assessments should be conducted. The consumption of fully cooked shellfish can significantly reduce the risk of HAV, RoV, NoV GI, and NoV GII infections. Furthermore, given the export value and importance of shellfish samples, their microbial quality and safety should be routinely monitored.

The effect of the waste disposal crisis on the rates of hospitalization due to acute diarrheal illness in a middle-income country: Retrospective chart review.

BACKGROUND: A waste disposal crisis occurred in Lebanon leading to the accumulation of garbage in the streets of Beirut. It is not clear whether such a limited crisis of improper waste disposal will lead to more diarrhea illnesses. The purpose of this study is to compare the rates of admissions due to diarrhea and characterize the illness among adults and children before, during and after the garbage crisis. MATERIALS AND METHODS: This study is an observational retrospective in-patient chart review extending over four time intervals: pre-crisis, major crisis, minor crisis, and post-crisis periods. Hospital admissions due to diarrhea or gastroenteritis were included. Descriptive analysis of the following was done: characteristics of the present illness at the time of admission, stool and lab tests ordered and management of the diarrhea illness. RESULTS: There was an increase in admission rates due to diarrhea in the months of the crisis as compared to pre and post-crisis months. The severity of diarrheal illness and the management of admitted patients were almost the same across the four periods. Rota virus diarrheal outbreak was noted during the major crisis among children less than 2 years old, and adults above 18 years old. CONCLUSIONS: Despite an increase in the rates of admissions due to diarrhea during the garbage crisis, the severity of the diarrhea illness did not differ. This may indicate that physicians and patients were more worried and utilized more health services. However, Rota outbreaks may be more prominent with improper garbage disposal.

Glutathione Transferase as a Potential Marker for Gut Epithelial Injury versus the Protective Role of Breast Milk sIgA in Infants with Rota Virus Gastroenteritis.

BACKGROUND: Secretory immunoglobulin A (SIgA) plays an important protective role in the recognition and clearance of enteric pathogens. AIM: This study was designed to assess if mucosal integrity "measured by secretory IgA (SIgA)" is a protective factor from more epithelial alteration "measured by glutathione transferase" in infants with Rota gastroenteritis and its relation to infants' feeding pattern. PATIENTS AND METHODS: This study was conducted on 79 infants aged 6 months and less from those diagnosed as having gastroenteritis and admitted to Gastroenteritis Department in Abo El Rish Pediatric Hospital, Cairo University. Plasma glutathione s-transferases and Stool SIgA were measured using ELISA technique. Rota virus detection was done by Reverse transcriptase PCR. RESULTS: SIgA was found to be significantly positive in exclusive breast fed infants, Glutathione transferase was significantly more frequently positive in Rota positive cases than Rota negative cases by Reverse transcriptase PCR. A significant negative correlation between Glutathione transferase and Secretory IgA was found, (p < 0.05). CONCLUSION: Breast feeding should be encouraged and highly recommended in the first two years of life as it provides Secretory IgA to breast fed infants who in turn protect them against epithelial damage caused by Rota viral gastroenteritis.

In silico study of potential autoimmune threats from rotavirus infection.

Rotavirus, the major cause of infantile nonbacterial diarrhea, was found to be associated with development of diabetes-associated auto-antibodies. In our study we tried to find out further potential autoimmune threats of this virus using bioinformatics approach. We took rotaviral proteins to study similarity with Homo sapiens proteome and found most conserved structural protein VP6 matches at two regions with ryanodine receptor, an autoimmune target associated with myasthenia gravis. Myasthenia gravis, a chronic neurodegenerative autoimmune disorder with no typical known reason, is characterized by fluctuating muscle weakness which is typically enhanced during muscular effort. Affected patients generate auto antibodies against mainly acetyl choline receptor and sarcoplasmic reticulum calcium-release channel protein ryanodine receptor. Further, we observed that two regions which matched with ryanodine receptor remain conserved in all circulating rotaviral strains and showed significant antigenecity with respect to myasthenia gravis associated HLA haplotypes. Overall, our study detected rotaviral VP6 as a potential threat for myasthenia gravis and enlighten an area of virus associated autoimmune research.

Mucosal Immunity and acute viral gastroenteritis.

Acute gastroenteritis is a major killer of the very young worldwide. Rotavirus is the most common intestinal virus, causing acute gastroenteritis and extra-intestinal complications especially in young and chronically ill subjects. As early as 1991, the WHO recommended as high priority the development of a vaccine against rotavirus, the major pathogen causing enteric infections. Since the introduction of rotavirus vaccines for infant immunization programmes in different parts of the world in 2006, vaccination against rotavirus has resulted in substantial declines in severe gastroenteritis. The oral rotavirus vaccines RotaTeq(®) and Rotarix(®) are excellent examples for their unique features and principles of mucosal immunization. We elaborate on rotavirus immunity and the success of rotavirus vaccination and aspects also beyond infants' acute gastroenteritis.

Mucosal immunization in perspective.

Pandemic and seasonal influenza viruses are a constant public health threat with substantial morbidity and mortality worldwide. Prophylaxis is hard to realize, but immunization provides an efficient tool to control the disease. Despite most infections occurring at or through mucosal surfaces, vaccines are predominantly administered parenterally. Recently it has been suggested that vaccines applied via mucosal surfaces may be a viable novel approach. A number of clinical studies have proven live attenuated influenza vaccine given intranasally to have equivalent or superior immunogenicity and efficacy at the upper and lower respiratory tract compared with systemic intramuscular vaccination. Intranasal application provides easy administration facilitating mass immunization campaigns which requires no strictly sterile injection and is painless to recipients.

Hepatitis por Rotavirus: A propósito de un caso / Rotavirus hepatitis: About a case

Introducción: La infección por rota virus se ha reconocido como un patógeno en el humano y causante de enfermedad diarreica grave; la infección se ha asociado con meningitis aséptica, enterocolitis necrotizante, miositis aguda , abscesos hepáticos, neumonía, enfermedad de Kawasaki , síndrome de muerte súbita y enfermedad de Crohn. En este caso se informa la asociación con hepatitis infecciosa. Caso Clínico: Masculino, con colitis alérgica a las proteínas de la leche de vaca y de la soya, desde el nacimiento. Al mes y medio presenta enfermedad diarreica por rota virus asociada a hepatitis. Conclusiones: Tradicionalmente se ha considerado que es una infección del intestino delgado y que todos los eventos fisiopatologicos son el resultado de este tipo de lesión y de la intolerancia alimentaria que provoca a nivel local, sin embargo se han informado enfermedades que se presentan en otros órganos, como respuesta a la infección viral del sistema digestivo. En esta ocasión la invasión por el virus provoca daño hepático.

We describe a 3- month-old infant that was diagnosed with hepatitis secondary to infection by the rotavirus. The rotavirus has been recognized as a human pathogen that has been associated with severe diarrhea, aseptic meningitis, necrotizing enterocolitis, acute miositis, hepatic abscesses, pneumonia, Kawasaki disease, sudden infant death syndrome, and inflammatory bowel disease such as Crohn’s. To our knowledge, only three reports have associated the rotavirus with acute hepatitis, however, one of the three reports was in an immunodeficient patient. In this report we present another case of acute hepatitis secondary to the rotavirus, in an immunocompetent patient.