Autohesion Mechanisms at Interfaces Between Random Copolymer Melts: Mesoscopic Simulations with Realistic Coarse-Grained Models.

The increase in welding time during the interdiffusion of a pair of non reacting random copolymer melts favors the strength rate of healing at the interface. Furthermore, the diffusion kinetic during the interpenetration of copolymer chains across the interface is strongly dependent on molecular weight. In this paper we perform mesoscopic simulations with realistic coarse grain models to study the autohesion mechanism across the interface between slightly entangled styrene-butadiene random copolymer melts.

Enhancing sequencing batch reactors for efficient wastewater treatment across diverse applications: A comprehensive review.

This review explores recent progress in sequencing batch reactors (SBRs) and hybrid systems for wastewater treatment, emphasizing their adaptability and effectiveness in managing diverse wastewater compositions. Through extensive literature analysis from 1985 to 2024, the integration of advanced technologies like photocatalysis within hybrid systems is highlighted, showing promise for improved pollutant removal efficiencies. Insights into operational parameters, reactor design, and microbial communities influencing SBR performance are discussed. Sequencing batch biofilm reactors (SBBRs) demonstrate exceptional efficiency in Chemical Oxygen Demand, nitrogen, and phosphorus removal, while innovative anaerobic-aerobic-anoxic sequencing batch reactors (AOA-SBRs) offer effective nutrient removal strategies. Hybrid systems, particularly photocatalytic sequencing batch reactors (PSBRs), show potential for removing persistent pollutants like antibiotics and phenols, underscoring the significance of advanced oxidation processes. However, research gaps persist, including the need for comparative studies between different SBR types and comprehensive evaluations of long-term performance, environmental variability, and economic viability. Addressing these gaps will be vital for the practical deployment of SBRs and hybrid systems. Further exploration of synergies, economic considerations, and reactor stability will enhance the sustainability and scalability of these technologies for efficient and eco-friendly wastewater treatment.

Rapid and specific detection of Pentastiridius leporinus by recombinase polymerase amplification assay.

Pentastiridius leporinus (Hemiptera: Cixiidae) is the main vector of an emerging and fast spreading sugar beet disease, the syndrome 'basses richesses' (SBR), in different European countries. The disease is caused by the γ-3-proteobacterium 'Candidatus Arsenophonus phytopathogenicus' and the phytoplasma 'Candidatus Phytoplasma solani' which are exclusively transmitted by planthoppers and can lead to a significant loss of sugar content and yield. Monitoring of this insect vector is important for disease management. However, the morphological identification is time consuming and challenging as two additional cixiid species Reptalus quinquecostatus and Hyalesthes obsoletus with a very close morphology have been reported in sugar beet fields. Further, identification of females and nymphs of P. leporinus at species level based on taxonomic key is not possible. In this study, an isothermal nucleic acid amplification based on recombinase polymerase amplification (RPA) was developed to specifically detect P. leporinus. In addition, real-time RPA was developed to detect both adults (male and female) and nymph stages using pure or crude nucleic acid extracts. The sensitivity of the real-time RPA for detection of P. leporinus was comparable to real-time PCR, but a shorter time (< 7 min) was required. This is a first report for real-time RPA application for P. leporinus detection using crude nucleic acid templates which can be applied for fast and specific detection of this vector in the field.

Effect of Fillers Modification with ILs on Fillers Textural Properties: Thermal Properties of SBR Composites.

In this work, we present the effect of graphene nanoplatelets (GnPs) modification with ionic liquids (ILs). The textural properties of graphene nanoplatelets (GnPs) used as styrene-butadiene rubber's filler and the thermal properties of the composites obtained with the use of the mentioned fillers were investigated. GnPs were modified with 1-butylpyridinium bromide (BPyBr) and 4-methyl-1-butylpyridinium bromide (BmPyBr) through two different ways. One strategy has been to deposit the filler modifier from the solution. The second one involved the modification of the filler with ionic liquids in bulk during the preparation of elastomer blends. Settlement of the proposed ionic liquids onto the GnPs' surface led to significant changes in the textural characteristics. BPyBr has restricted the filler's microporosity, whereas BmPyBr has caused the formation of a more opened filler structure without the increase in its average pore size. GnPs modified with ILs led to reducing the temperature of vulcanization of SBR compounds and affected the thermal stability of the composites.

Effluent quality improvement in sequencing batch reactor-based wastewater treatment processes using advanced control strategies.

The treatment of wastewater is highly challenging due to large fluctuations in flowrates, pollutants, and variable influent water compositions. A sequencing batch reactor (SBR) and modified SBR cycle-step-feed process (SSBR) configuration are studied in this work to effectively treat municipal wastewater while simultaneously removing nitrogen and phosphorus. To control the amount of dissolved oxygen in an SBR, three axiomatic control strategies (proportional integral (PI), fractional proportional integral (FPI), and fuzzy logic controllers) are presented. Relevant control algorithms have been designed using plant data with the models of SBR and SSBR based on ASM2d framework. On comparison, FPI showed a significant reduction in nutrient levels and added an improvement in effluent quality. The overall effluent quality is improved by 0.86% in FPI in comparison with PI controller. The SSBR, which was improved by precisely optimizing nutrient supply and aeration, establishes a delicate equilibrium. This refined method reduces oxygen requirements while reliably sustaining important biological functions. Focusing solely on the FPI controller's performance in terms of total air volume consumption, the step-feed SBR mechanism achieves an excellent 11.04% reduction in consumption.

A Highly Bright Near-Infrared Afterglow Luminophore for Activatable Ultrasensitive In Vivo Imaging.

Afterglow luminescence imaging probes, with long-lived emission after cessation of light excitation, have drawn increasing attention in biomedical imaging field owing to their elimination of autofluorescence. However, current afterglow agents always suffer from an unsatisfactory signal intensity and complex systems consisting of multiple ingredients. To address these issues, this study reports a near-infrared (NIR) afterglow luminophore (TPP-DO) by chemical conjugation of an afterglow substrate and a photosensitizer acting as both an afterglow initiator and an energy relay unit into a single molecule, resulting in an intramolecular energy transfer process to improve the afterglow brightness. The constructed TPP-DO NPs emit a strong NIR afterglow luminescence with a signal intensity of up to 108  p/s/cm2 /sr at a low concentration of 10 µM and a low irradiation power density of 0.05 W/cm2 , which is almost two orders of magnitude higher than most existing organic afterglow probes. The highly bright NIR afterglow luminescence with minimized background from TPP-DO NPs allows a deep tissue penetration depth ability. Moreover, we develop a GSH-activatable afterglow probe (Q-TPP-DO NPs) for ultrasensitive detection of subcutaneous tumor with the smallest tumor volume of 0.048 mm3 , demonstrating the high potential for early diagnosis and imaging-guided surgical resection of tumors.

Quorum sensing unveils the sludge floccule-assisted stabilization of aerobic granules in granule-dominated sequencing batch reactors.

Floccules are another major form of microbial aggregates in aerobic granular sludge systems. Previous studies mainly attributed the persistence of floccules to their relatively faster nutrient uptake and higher growth rate over aerobic granules; however, they failed to unravel the underlying mechanism of the long-term coexistence of these two aggregates. In this work, the existence and function of the floccules in an aerobic granule-dominated sequencing batch reactor were investigated from the view of quorum sensing (QS) and quorum quenching (QQ). The results showed that though the floccules were closely associated with the granules in terms of similar community structures (including the QS- and QQ-related ones), they exhibited a relatively higher QQ-related activity but a lower QS-related activity. A compatible proportion of floccules might be helpful to maintain the QS-related activity and keep the granules stable. In addition, the structure difference was demonstrated to diversify the QS- and QQ-related activities of the floccules and the aerobic granules. These findings could broaden our understanding of the interactions between the coexistent floccules and granules in aerobic granule-dominated systems and would be instructive for the development of the aerobic granular sludge process.

Biological degradation and mineralization of tetracycline antibiotic using SBR equipped with a vertical axially rotating biological bed (SBR-VARB).

Tetracycline (TC) is a widely used antibiotic with a complex aromatic chemical structure and is highly resistant to biodegradation. In this study, an SBR equipped with a vertical axially rotating biological bed (SBR-VARB) was used for the biodegradation and mineralization of TC. SBR-VARB showed high efficiency in removing TC (97%), total phenolic compounds (TP) (95%), and COD (85%) under optimal operating conditions (TC = 50 mg/L, HRT = 1.75 d, and OLR = 36 g COD/m3 d). The SBR-VARB was able to treat higher concentrations of TC in shorter HRT than reported in previous studies. The contribution of VARB to improve SBR efficiency in removing TC, TP, and COD was 16, 36, and 48%, respectively. Intermediate compounds formed during the biodegradation of TC were identified using GC-MS under the optimal operating conditions of the bioreactor. These are mainly organic compounds with linear chemical structures. Based on the complete biodegradation of TC under the optimal operating conditions of the bioreactor, 93% and 36% of the chlorine and nitrogen atoms in the chemical structure of TC appeared in the wastewater, respectively. According to the sequence analysis of 16SrDNA, Pseudomonas sp., Kocuria Polaris, and Staphylococcus sp. were identified in the biofilm of VARB and the suspended biomass of the bioreactor. Therefore, SBR-VARB showed high efficiency in the biodegradation and mineralization of TC and can be used as a suitable option for treating wastewater containing antibiotics and other toxic compounds.

Effect of Cross-Linking Density on Non-Linear Viscoelasticity of Vulcanized SBR: A MD Simulation and Experimental Study.

In recent years, there has been a growing interest in changes in dynamic mechanical properties of mixed rubber during dynamic shear, yet the influence of vulcanized characteristics on the dynamic shear behavior of vulcanized rubber, particularly the effect of cross-linking density, has received little attention. This study focuses on styrene-butadiene rubber (SBR) and aims to investigate the impact of different cross-linking densities (Dc) on dynamic shear behavior using molecular dynamics (MD) simulations. The results reveal a remarkable Payne effect, where the storage modulus experiences a significant drop when the strain amplitude (γ0) exceeds 0.1, which can be attributed to the fracture of the polymer bond and the decrease in the molecular chain's flexibility. The influence of various Dc values mainly resides at the level of molecular aggregation in the system, where higher Dc values impede molecular chain motion and lead to an increase in the storage modulus of SBR. The MD simulation results are verified through comparisons with existing literature.

Dynamic Volumetric Imaging of Mouse Cerebral Blood Vessels In Vivo with an Ultralong Anti-Diffracting Beam.

Volumetric imaging of a mouse brain in vivo with one-photon and two-photon ultralong anti-diffracting (UAD) beam illumination was performed. The three-dimensional (3D) structure of blood vessels in the mouse brain were mapped to a two-dimensional (2D) image. The speed of volumetric imaging was significantly improved due to the long focal length of the UAD beam. Comparing one-photon and two-photon UAD beam volumetric imaging, we found that the imaging depth of two-photon volumetric imaging (80 µm) is better than that of one-photon volumetric imaging (60 µm), and the signal-to-background ratio (SBR) of two-photon volumetric imaging is two times that of one-photon volumetric imaging. Therefore, we used two-photon UAD volumetric imaging to perform dynamic volumetric imaging of mouse brain blood vessels in vivo, and obtained the blood flow velocity.

Retrofitting a full-scale multistage landfill leachate treatment plant by introducing coagulation/flocculation/sedimentation and ultrafiltration process steps.

Considering that landfilling still remains among the most commonly used methods for the confrontation of solid wastes, effective methods should be applied to treat the leachate generated, due to its recalcitrant nature. In this work, a full-scale system consisting of two SBRs operating in parallel (350 m3 each) and two activated carbon (AC) columns operating in series (3 m3 each) was retrofitted by introducing a coagulation/flocculation/sedimentation (C/F/S) unit of 7.8 m3 and an ultrafiltration (UF) membrane of 100 m2 to effectively treat landfill leachate. The raw leachate was characterized by high COD and NH4+-N concentration, i.e., 3095 ± 706 mg/L and 1054 ± 141 mg/L respectively, a BOD/COD ratio of 0.22, and high concentrations of certain heavy metals. Leachate processing in this retrofitted multistage treatment system resulted in total COD removal efficiency of 89.84%, with biological treatment, C/F, UF, and AC contributing 46.31%, 4.68%, 15.98%, and 22.87% to the overall organic content removal. The retrofitted scheme achieved an overall NH4+-N and TKN removal of 92.03% and 91.75% respectively, attributed mostly to the activity of an effective nitrifying community. Color number (CN) was reduced by 26.96%, 10.29%, 15.94%, and 5.39% after the activated sludge, the C/F, the UF, and the AC adsorption process respectively, corresponding to a 58.91% overall decrease. Regarding heavy metal removal, all elements examined, apart from Ni, i.e., effluent As, Cd, Co, Cr, Cu, Hg, Mg, Mn, and Pb, were below the legislative limits set by the national authorities for restricted or unrestricted irrigation. Lastly, total operating expenses (OPEX) were estimated as equal to 72,687 €/year or 6.64 €/m3.

Effects of sulphur amino acids on the size and structure of microbial communities of aerobic granular sludge bioreactors.

Granular activated sludge has been described as a promising tool in treating wastewater. However, the effect of high concentrations of sulphur amino acids, cysteine and methionine, in the evolution, development and stability of AGS-SBRs (aerobic granular sludge in sequential batch reactors) and their microbial communities is not well-established. Therefore, this study aimed to evaluate microbial communities' size, structure and dynamics in two AGS-SBRs fed with two different concentrations of amino acids (50 and 100 mg L-1 of both amino acids). In addition, the impact of the higher level of amino acids was also determined under an acclimatization or shock strategy. While N removal efficiency decreased with amino acids, the removal of the organic matter was generally satisfactory. Moreover, the abrupt presence of both amino acids reduced even further the removal performance of N, whereas under progressive adaptation, the removal yield was higher. Besides, excellent removal rates of cysteine and methionine elimination were found, in all stages below 80% of the influent values. Generally considered, the addition of amino acids weakly impacts the microbial communities' total abundances. On the contrary, the presence of amino acids sharply modulated the dominant bacterial structures. Furthermore, the highest amino acid concentration under the shock strategy resulted in a severe change in the structure of the microbial community. Acidovorax, Flavobacterium, Methylophilus, Stenotrophomonas and Thauera stood out as the prominent bacteria to cope with the high presence of cysteine and methionine. Hence, the AGS-SBR technology is valuable for treating influents enriched in sulphur Aa inclusively when a shock strategy was used.

Influence of temperature on aerobic granular sludge formation and stability treating municipal wastewater with high nitrogen loadings.

This study investigated the influence of temperature (20 and 30 °C) on the formation and stability of aerobic granules in sequential batch reactors (SBR). Therefore, two lab-scale SBRs operated at 20 and 30 °C (SBR20 and SBR30) were used. The reactors were fed with municipal wastewater (CODt:TN:TP 100:15:1.7), leading to mean organic loading rates (OLR) of 1.3 ± 0.4 kgCODt m-3 day-1. Both reactors had the same height/diameter ratio of 4.2 and were inoculated with activated sludge from a municipal wastewater treatment plant. The operational conditions were also the same for both temperatures and lasted in stable process parameters for over 100 days. By optimizing the aeration and oxygen concentration, a high removal efficiency of NH4-N (∼99%) and COD (∼90%) was achieved in both reactors, despite the poor C:N:P ratio at the influent. Furthermore, a relatively low oxygen concentration of 2 mg L-1 was defined as the set point for the control strategy. Nevertheless, granulation at 30 °C was significantly faster, resulting in more stable sludge volume index (SVI) values (SVI10/SVI30 < 1.1). The granules formed at 30 °C were also larger, more compact, and considerably more stable against system disturbances. However, at higher temperatures, larger granules might be required for nitrate removal because of the increased oxygen diffusion rates. Finally, microbiological 16S rRNA gene amplicon analysis for both systems indicated major differences relatively to the inoculum sludge only for nitrogen-degrading organisms.

Investigating the biological degradation of the drug ß-blocker atenolol from wastewater using the SBR.

Drug compounds are one of the main contributors to the entry of micro-pollutants into the environment, known as a constant threat to environmental stability. Atenolol is a type of beta-blocker extensively used to cure cardiovascular disorders. The residues of this compound have been continuously detected in aquatic environments because it is a polar and poorly biodegradable compound. Thus, removing atenolol from wastewater is essential before discharging into the environment. Biological processes are considered the most important removal process for polar drugs in wastewater treatment plants. Accordingly, for the first time in this study, the SBR performance was investigated in the biodegradation and mineralization of atenolol under different concentrations (50-600 mg/L) and hydraulic retention times (48-32 h). Based on the results, the time required for the acclimation of biomass to atenolol (C: 50 mg/L and the HRT: 48 h) was 80 days. The SBR efficiencies under optimum conditions (C: 400 mg/L and HRT: 40 h) in removing the atenolol and COD were 91% and 87%, respectively. For the first time in this study, one of the main pathways of the atenolol biodegradation was identified. Based on the review and comparison of the results of this study with existing literature showing that the SBR used in this study was able to remove higher concentrations with better efficiencies than other processes. Therefore, it can be concluded that the SBR used in this study could be considered an efficient and promising technique for treating wastewaters containing atenolol and other beta-blocker group drugs.

Reducing excess sludge volume in sequencing batch reactor by integrating ultrasonic waves and ozonation.

The effects of ultrasonic waves and ozonation on the reduction of produced sludge in the sequencing batch reactor (SBR) system were investigated in laboratory-scale experiments. For this purpose, the optimal ozone dosage was determined by measuring soluble chemical oxygen demand (SCOD), protein concentration, turbidity level, and biomass yield coefficient. Next, the effect of its integration with different levels of ultrasonic specific energy was evaluated. Based on the results, the minimum excess sludge production in the SBR system was achieved at the ozone dosage of 11 mg O3/g MLSS followed by ultrasonic specific energy of 12000 kJ/kg TS. In this case, the biomass yield coefficient decreased from 0.75 in the control reactor to 0.34 mg MLSS/mg COD in the test reactor, which was equal to a 54% reduction in excess sludge production in the SBR system. In these circumstances, the removal efficiencies of COD, total nitrogen, and total phosphorus were measured as 90%, 82%, and 81%, respectively.

An Assessment of Mass Flows, Removal and Environmental Emissions of Bisphenols in a Sequencing Batch Reactor Wastewater Treatment Plant.

This study analyzed 16 bisphenols (BPs) in wastewater and sludge samples collected from different stages at a municipal wastewater treatment plant based on sequencing batch reactor technology. It also describes developing an analytical method for determining BPs in the solid phase of activated sludge based on solid-phase extraction and gas chromatography-mass spectrometry. Obtained concentrations are converted into mass flows, and the biodegradation of BPs and adsorption to primary and secondary sludge are determined. Ten of the sixteen BPs were present in the influent with concentrations up to 434 ng L-1 (BPS). Only five BPs with concentrations up to 79 ng L-1 (BPA) were determined in the plant effluent, accounting for 8 % of the total BPs determined in the influent. Eleven per cent of the total BPs were adsorbed on primary and secondary sludge. Overall, BPs biodegradation efficiency was 81%. The highest daily emissions via effluent release (1.48 g day-1) and sludge disposal (4.63 g day-1) were for BPA, while total emissions reached 2 g day-1 via effluent and 6 g day-1 via sludge disposal. The data show that the concentrations of BPs in sludge are not negligible, and their environmental emissions should be monitored and further studied.

Activated sludge microbial communities and hydrolytic potential in a full-scale SBR system treating landfill leachate.

Landfill leachate, due to its recalcitrant nature and toxicity, poses a serious environmental threat, which requires the implementation of effective treatment processes. In this work, a full-scale treatment system consisting of two Sequencing Batch Reactors (SBRs) was used for the processing of landfill leachate of intermediate to mature age (BOD/COD ratio of 0.16). Biosystem operation resulted in BOD5, COD and TKN removal efficiencies of 81%, 39% and 76%, respectively, whereas the low residual NO3--N concentration in the effluent (4.01 ± 0.10 mg/L) was indicative of the efficient denitrification process. Assessment of hydrolytic potential of activated sludge revealed high endocellular and extracellular lipase activities, which reached values up to 206 and 141 U/g protein respectively, possibly as the consequence of plastics degradation during maturation process. Implementation of Illumina sequencing indicated the predominance of Alphaproteobacteria, accompanied by members of Bacteroidetes, Betaproteobacteria and Chloroflexi. Paracoccus was the predominant genus identified, followed by representatives of the genera Bellilinea, Flavobacterium, Thauera and Truepera. Nitrosomonas was the major ammonia-oxidizing bacterium (AOB), while nitrite oxidation was mainly achieved by the uncultured nitrite-oxidizing bacterium (NOB) Candidatus Nitrotoga.

SARS-CoV-2 in environmental perspective: Occurrence, persistence, surveillance, inactivation and challenges.

The unprecedented global spread of the severe acute respiratory syndrome (SARS) caused by SARS-CoV-2 is depicting the distressing pandemic consequence on human health, economy as well as ecosystem services. So far novel coronavirus (CoV) outbreaks were associated with SARS-CoV-2 (2019), middle east respiratory syndrome coronavirus (MERS-CoV, 2012), and SARS-CoV-1 (2003) events. CoV relates to the enveloped family of Betacoronavirus (ßCoV) with positive-sense single-stranded RNA (+ssRNA). Knowing well the persistence, transmission, and spread of SARS-CoV-2 through proximity, the faecal-oral route is now emerging as a major environmental concern to community transmission. The replication and persistence of CoV in the gastrointestinal (GI) tract and shedding through stools is indicating a potential transmission route to the environment settings. Despite of the evidence, based on fewer reports on SARS-CoV-2 occurrence and persistence in wastewater/sewage/water, the transmission of the infective virus to the community is yet to be established. In this realm, this communication attempted to review the possible influx route of the enteric enveloped viral transmission in the environmental settings with reference to its occurrence, persistence, detection, and inactivation based on the published literature so far. The possibilities of airborne transmission through enteric virus-laden aerosols, environmental factors that may influence the viral transmission, and disinfection methods (conventional and emerging) as well as the inactivation mechanism with reference to the enveloped virus were reviewed. The need for wastewater epidemiology (WBE) studies for surveillance as well as for early warning signal was elaborated. This communication will provide a basis to understand the SARS-CoV-2 as well as other viruses in the context of the environmental engineering perspective to design effective strategies to counter the enteric virus transmission and also serves as a working paper for researchers, policy makers and regulators.

Morphology and Physico-Mechanical Threshold of α-Cellulose as Filler in an E-SBR Composite.

In the current context of green mobility and sustainability, the use of new generation natural fillers, namely, α-cellulose, has gained significant recognition. The presence of hydroxyl groups on α-cellulose has generated immense eagerness to map its potency as filler in an elastomeric composite. In the present work, α-cellulose-emulsion-grade styrene butadiene rubber (E-SBR) composite is prepared by conventional rubber processing method by using variable proportions of α-cellulose (1 to 40 phr) to assess its reinforce ability. Rheological, physical, visco-elastic and dynamic-mechanical behavior have clearly established that 10 phr loading of α-cellulose can be considered as an optimized dosage in terms of performance parameters. Morphological characterization with the aid of scanning electron microscope (SEM) and transmission electron microscopy (TEM) also substantiated that composite with 10 phr loading of α-cellulose has achieved the morphological threshold. With this background, synthetic filler (silica) is substituted by green filler (α-cellulose) in an E-SBR-based composite. Characterization of the compound has clearly established the reinforcement ability of α-cellulose.

Comparison of the effects of salinity on microbial community structures and functions in sequencing batch reactors with and without carriers.

This study investigated and compared the microbial communities between a sequencing batch reactor (SBR) without carriers and a hybrid SBR with addition of carriers for the treatment of saline wastewater. The two systems were operated over 292 days with alternating aerobic/anoxic mode (temperature: 28℃, salinity: 0.0-3.0%). High removal efficiency of chemical oxygen demand (COD) and total inorganic nitrogen (TIN) was achieved in both the SBR (above 86.7 and 95.4% respectively) and hybrid SBR (above 84.4 and 94.0%) at 0.0-2.5% salinity. Further increasing salinity to 3.0% decreased TIN removal efficiency to 78.4% in the hybrid SBR. Steep decline of biodiversity and relative abundance of ammonia-oxidizing bacteria (AOB) contributed to the worse performance. More genera related to sulfide-oxidizing and sulfate-reducing bacteria were detected in the hybrid SBR than the SBR at 3.0% salinity. The abundance of halotolerant bacteria increased with the salinity increase for both reactors, summing up to 25.5% in the suspended sludge (S-sludge) from the SBR, 28.9 and 22.9% in the S-sludge and biofilm taken from the hybrid SBR, respectively. Nitrification and denitrification via nitrate was the main nitrogen removal pathway in the SBR and hybrid SBR at 0.0 and 0.5% salinity, while partial nitrification and denitrification via nitrite became the key process for nitrogen removal in the two reactors when the salinity was increased to 1.0-3.0%. Higher abundance of anaerobic ammonium-oxidizing (ANAMMOX) and sulfide-oxidizing autotrophic denitrification (SOAD) bacteria were found in the hybrid SBR at 3.0% salinity.