Mammalian octopus cells are direction selective to frequency sweeps by excitatory synaptic sequence detection.

Octopus cells are remarkable projection neurons of the mammalian cochlear nucleus, with extremely fast membranes and wide-frequency tuning. They are considered prime examples of coincidence detectors but are poorly characterized in vivo. We discover that octopus cells are selective to frequency sweep direction, a feature that is absent in their auditory nerve inputs. In vivo intracellular recordings reveal that direction selectivity does not derive from across-frequency coincidence detection but hinges on the amplitudes and activation sequence of auditory nerve inputs tuned to clusters of hot spot frequencies. A simple biophysical octopus cell model excited with real nerve spike trains recreates direction selectivity through interaction of intrinsic membrane conductances with the activation sequence of clustered excitatory inputs. We conclude that octopus cells are sequence detectors, sensitive to temporal patterns across cochlear frequency channels. The detection of sequences rather than coincidences is a much simpler but powerful operation to extract temporal information.

Sensitivity to Frequency Modulation is Limited Centrally.

Modulations in both amplitude and frequency are prevalent in natural sounds and are critical in defining their properties. Humans are exquisitely sensitive to frequency modulation (FM) at the slow modulation rates and low carrier frequencies that are common in speech and music. This enhanced sensitivity to slow-rate and low-frequency FM has been widely believed to reflect precise, stimulus-driven phase locking to temporal fine structure in the auditory nerve. At faster modulation rates and/or higher carrier frequencies, FM is instead thought to be coded by coarser frequency-to-place mapping, where FM is converted to amplitude modulation (AM) via cochlear filtering. Here, we show that patterns of human FM perception that have classically been explained by limits in peripheral temporal coding are instead better accounted for by constraints in the central processing of fundamental frequency (F0) or pitch. We measured FM detection in male and female humans using harmonic complex tones with an F0 within the range of musical pitch but with resolved harmonic components that were all above the putative limits of temporal phase locking (>8 kHz). Listeners were more sensitive to slow than fast FM rates, even though all components were beyond the limits of phase locking. In contrast, AM sensitivity remained better at faster than slower rates, regardless of carrier frequency. These findings demonstrate that classic trends in human FM sensitivity, previously attributed to auditory nerve phase locking, may instead reflect the constraints of a unitary code that operates at a more central level of processing.SIGNIFICANCE STATEMENT Natural sounds involve dynamic frequency and amplitude fluctuations. Humans are particularly sensitive to frequency modulation (FM) at slow rates and low carrier frequencies, which are prevalent in speech and music. This sensitivity has been ascribed to encoding of stimulus temporal fine structure (TFS) via phase-locked auditory nerve activity. To test this long-standing theory, we measured FM sensitivity using complex tones with a low F0 but only high-frequency harmonics beyond the limits of phase locking. Dissociating the F0 from TFS showed that FM sensitivity is limited not by peripheral encoding of TFS but rather by central processing of F0, or pitch. The results suggest a unitary code for FM detection limited by more central constraints.

Estimation of local variation in Young's modulus over a gold nanocontact using microscopic nanomechanical measurement method.

Nanoscale materials tend to have a single crystal domain, leading to not only size dependence but also orientation dependence of their mechanical properties. Recently, we developed a microscopic nanomechanical measurement method (MNMM), which enabled us to obtain equivalent spring constants (force gradients) of nanocontacts while observing their atomic structures by transmission electron microscopy (TEM). Therein, we evaluated Young's modulus based on a model that a newly introduced layer at the thinnest section of a nanocontact determined the change in the measured equivalent spring constant, and discussed their size dependence. However, this model is not general for other nanomaterials that do not exhibit the introduction of a new atomic layer while stretching. In this study, using MNMM, we propose a new analytical method to directly retrieve the local Young's modulus of nanomaterials by measuring initial lattice spacing and its displacement of a local region in the TEM image during the stretching of the nanocontact. This reveals the size dependence of local Young's modulus at various positions of the nanocontact at once. As a result, our estimated Young's modulus for a gold [111] nanocontact showed a size dependence similar to the one previously reported. This indicates that this analytical method benefits in revealing the mechanical properties of not only nanomaterials but also structurally heterogeneous materials such as high-entropy alloys. .

Optimization of tumor-treating field therapy for triple-negative breast cancer cells in vitro via frequency modulation.

PURPOSE: Currently, tumor-treating field (TTField) therapy utilizes a single "optimal" frequency of electric fields to achieve maximal cell death in a targeted population of cells. However, because of differences in cell size, shape, and ploidy during mitosis, optimal electric field characteristics for universal maximal cell death may not exist. This study investigated the anti-mitotic effects of modulating electric field frequency as opposed to utilizing uniform electric fields. METHODS: We developed and validated a custom device that delivers a wide variety of electric field and treatment parameters including frequency modulation. We investigated the efficacy of frequency modulating tumor-treating fields on triple-negative breast cancer cells compared to human breast epithelial cells. RESULTS: We show that frequency-modulated (FM) TTFields are as selective at treating triple-negative breast cancer (TNBC) as uniform TTFields while having a greater efficacy for combating TNBC cell growth. TTField treatment at a mean frequency of 150 kHz with a frequency range of ± 10 kHz induced apoptosis in a greater number of TNBC cells after 24 h as compared to unmodulated treatment which led to further decreased cell viability after 48 h. Furthermore, all TNBC cells died after 72 h of FM treatment while cells that received unmodulated treatment were able to recover to cell number equivalent to the control. CONCLUSION: TTFields were highly efficacious against TNBC growth, FM TTFields showed minimal effects on epithelial cells similar to unmodulated treatment.

.Signal Transparency of Remote Microphone Technology in Pediatric Bone Conduction Device Users

INTRODUCTION: Competing noise in the environment negatively affects speech intelligibility, particularly when listening at a distance. This is especially true for children with hearing loss in classroom environments where the signal-to-noise ratio is often poor. Remote microphone technology has been shown to be highly beneficial at improving the signal-to-noise ratio in hearing device users. Children with bone conduction devices, however, often must rely on indirect transmission of the acoustic signal for commonly used classroom-based remote microphone listening (e.g., digital adaptive microphone) which may negatively affect speech intelligibility. There are no studies on the effectiveness of using a relay method of signal delivery using remote microphone technology to improve speech intelligibility in adverse listening environments in bone conduction device users. METHODS: Nine children with irresolvable conductive hearing loss and 12 adult controls with normal hearing were included for study. Controls were bilaterally plugged to simulate conductive hearing loss. All testing was conducted using the Cochlear™ Baha® 5 standard processor coupled with either the Cochlear™ Mini Microphone 2+ digital remote microphone or the Phonak Roger™ adaptive digital remote microphone. Speech intelligibility in noise was evaluated in the (1) bone conduction device processor alone, (2) bone conduction device + personal remote microphone, and (3) bone conduction device + personal remote microphone + adaptive digital remote microphone listening conditions at -10 dB, 0 dB, and +5 dB signal-to-noise ratios. RESULTS AND CONCLUSIONS: Speech intelligibility in noise improved significantly in the bone conduction device + personal remote microphone condition over the bone conduction device alone, demonstrating significant benefit for listening at poor signal-to-noise ratios in children with conductive hearing loss using bone conduction devices with personal remote microphone use. Experimental findings demonstrate poor signal transparency when using the relay method. Coupling of the adaptive digital remote microphone technology to the personal remote microphone negatively affects signal transparency, and no hearing in noise improvements are observed. Significant gains in speech intelligibility are consistently observed for direct streaming methods and are confirmed in adult controls. Behavioral findings are supported by objective verification of the signal transparency between the remote microphone and the bone conduction device.

A Modulated Approach for Improving MFSK RADARS to Resolve Mutual Interference on Autonomous Vehicles (AVs).

This paper proposes a novel automotive radar waveform involving the theory behind M-ary frequency shift key (MFSK) radar systems. Along with the MFSK theory, coding schemes are studied to provide a solution to mutual interference. The proposed MFSK waveform consists of frequency increments throughout the range of 76 GHz to 81 GHz with a step value of 1 GHz. Instead of stepping with a fixed frequency, a triangular chirp sequence allows for static and moving objects to be detected. Therefore, automotive radars will improve Doppler estimation and simultaneous range of various targets. In this paper, a binary coding scheme and a combined transform coding scheme used for radar waveform correlation are evaluated in order to provide unique signals. AVs have to perform in an environment with a high number of signals being sent through the automotive radar frequency band. Efficient coding methods are required to increase the number of signals that are generated. An evaluation method and experimental data of modulated frequencies as well as a comparison with other frequency method systems are presented.

Deconvolved Fractional Fourier Domain Beamforming for Linear Frequency Modulation Signals.

To estimate the direction of arrival (DOA) of a linear frequency modulation (LFM) signal in a low signal-to-noise ratio (SNR) hydroacoustic environment by a small aperture array, a novel deconvolved beamforming method based on fractional Fourier domain delay-and-sum beamforming (FrFB) was proposed. Fractional Fourier transform (FrFT) was used to convert the received signal into the fractional Fourier domain, and delay-and-sum beamforming was subsequently performed. Noise resistance was acquired by focusing the energy of the LFM signal distributed in the time-frequency domain. Then, according to the convolution structure of the FrFB complex output, the influence of the fractional Fourier domain complex beam pattern was removed by deconvolution, and the target spatial distribution was restored. Therefore, an improved spatial resolution of DOA estimation was obtained without increasing the array aperture. The simulation and experimental results show that, with a small aperture array at low SNR, the proposed method possesses higher spatial resolution than FrFB and frequency-domain deconvolved conventional beamforming.

Interactive Errors Analysis and Scale Factor Nonlinearity Reduction Methods for Lissajous Frequency Modulated MEMS Gyroscope.

Although the Lissajous frequency modulated (LFM) mode can improve the long-term and temperature stability of the scale factor (SF) for mode mismatch MEMS gyroscopes, its SF nonlinearity poses a significant limitation for full-scale accuracy maintenance. This paper examines the interaction effects among stiffness coupling, system phase delay, readout demodulation phase shift, and velocity amplitude mismatch within the control process. Based on the completion of frequency difference control and demodulation phase matching, we clarify that the remaining stiffness coupling and residual system phase error are the primary factors influencing SF nonlinearity. Furthermore, SF nonlinearity is reduced through error compensation. On one hand, this paper suppresses stiffness coupling through the observation of the instantaneous frequency difference and the application of the quadrature voltage. On the other hand, system phase error is compensated by observing the amplitude control force and tuning the reference in the Phase-Locked Loops (PLLs). Subsequent simulations of these methods demonstrated a remarkable 97% reduction in SF nonlinearity within the measurement range of ±500°/s. In addition, an observed rule dictates that maintaining a sufficiently large frequency split effectively constrains the SF nonlinearity.

Advancing Stepped-Waveform Radar Jamming Techniques for Robust False-Target Generation against LFM-CFAR Systems.

This study investigates the utilization of a stepped wave frequency modulation jamming technique in radar systems. The objective is to enhance the effectiveness and robustness of false target jamming in the presence of linear frequency modulation (LFM) radars employing constant false alarm rate (CFAR) detection. The proposed method combines stepped frequency modulation with full pulse delay/sum repeat jamming to enhance resilience against uncertainties in target parameters. Theoretical analysis and simulation experiments are conducted to establish relationships between key jammer parameters, such as frequency slope and power compensation, and performance metrics, like false target distribution and CFAR masking. The results demonstrate that the proposed technique effectively maintains a dense distribution of false targets surrounding the protected target, even in the presence of uncertainties in position and signal-to-noise ratio. In comparison to existing methods, the utilization of stepped-waveform modulation enables improved control over target distribution and CFAR masking. Adaptive power allocation compensates for parameter errors, thereby enhancing robustness. Simulation results reveal that the proposed approach significantly reduces the probability of detecting the true target by over 95% under uncertain conditions, while previous methods experienced degradation. The integration of stepped waveforms optimizes false target jamming, thereby advancing electronic warfare capabilities in countering advanced radar threats. This study establishes design principles for resilient jamming architectures and supports enhanced survivability against radars employing pulse compression and CFAR detection. Moreover, the concepts proposed in this study have the potential for extension to emerging radar waveforms.

Ultrasonic Energy and Data Transfer through a Metal-Liquid Multi-Layer Channel Enhanced by Automatic Gain and Carrier Control.

Ultrasonic communication and power transfer are attractive solutions when conventional electromagnetic-based or wired connections are unfeasible. Most ultrasonic communication applications concern a single-solid barrier. Nevertheless, some relevant scenarios can be composed of several fluid-solid media, through which communication and power transfer are intended. Due to its multi-layer nature, insertion loss and, consequently, the system efficiency considerably decrease. This paper presents an ultrasonic system capable of simultaneously power transferring and transmitting data through a set of two flat steel plates separated by a fluid layer using a pair of co-axially aligned piezoelectric transducers on opposite sides of the barrier. The system is based on frequency modulation and adopts a novel technique for automatic gain and automatic carrier control. The modems used herein were developed specifically for this application, rendering the system able to transfer data at a rate of 19,200 bps, using the frequency shift keying (FSK) modulation scheme and simultaneously transferring 66 mW of power through two flat steel plates (5 mm) separated by a fluid layer (100 mm), which completely supplied a pressure and temperature sensor. The proposed automatic gain control allowed a higher data transmission rate and the automatic carrier control reduced power consumption. The former reduced the transmission error from 12% to 5%, while the latter reduced the global power consumption from 2.6 W to 1.2 W. The proposed system is promising for monitoring applications such as oil wellbore structural health monitoring systems.

Integrated Low-Voltage Compliance and Wide-Dynamic Stimulator Design for Neural Implantable Devices.

In this study, a pulse frequency modulation (PFM)-based stimulator is proposed for use in biomedical implantable devices. Conventionally, functional electrical stimulation (FES) techniques have been used to reinforce damaged nerves, such as retina tissue and brain tissue, by injecting a certain amount of charge into tissues. Although several design methods are present for implementing FES devices, an FES stimulator for retinal implants is difficult to realize because of the chip area, which needs to be inserted in a fovea, sized 5 mm x 5 mm, and power limitations to prevent the heat generation that causes tissue damage. In this work, we propose a novel stimulation structure to reduce the compliance voltage during stimulation, which can result in high-speed and low-voltage operation. A new stimulator that is composed of a modified high-speed PFM, a 4-bit counter, a serializer, a digital controller, and a current driver is designed and verified using a DB HiTek standard 0.18 µm process. This proposed stimulator can generate a charge up to 130 nC, consumes an average power of 375 µW during a stimulation period, and occupies a total area of 700 µm × 68 µm.

A Phase-Sensitive Optical Time Domain Reflectometry with Non-Uniform Frequency Multiplexed NLFM Pulse.

In the domain of optical fiber distributed acoustic sensing, the persistent challenge of extending sensing distances while concurrently improving spatial resolution and frequency response range has been a complex endeavor. The amalgamation of pulse compression and frequency division multiplexing methodologies has provided certain advantages. Nevertheless, this approach is accompanied by the drawback of significant bandwidth utilization and amplified hardware investments. This study introduces an innovative distributed optical fiber acoustic sensing system aimed at optimizing the efficient utilization of spectral resources by combining compressed pulses and frequency division multiplexing. The system continuously injects non-linear frequency modulation detection pulses spanning various frequency ranges. The incorporation of non-uniform frequency division multiplexing augments the vibration frequency response spectrum. Additionally, nonlinear frequency modulation adeptly reduces crosstalk and enhances sidelobe suppression, all while maintaining a favorable signal-to-noise ratio. Consequently, this methodology substantially advances the spatial resolution of the sensing system. Experimental validation encompassed the multiplexing of eight frequencies within a 120 MHz bandwidth. The results illustrate a spatial resolution of approximately 5 m and an expanded frequency response range extending from 1 to 20 kHz across a 16.3 km optical fiber. This achievement not only enhances spectral resource utilization but also reduces hardware costs, making the system even more suitable for practical engineering applications.

Experimental Demonstration of a Visible Light Communications System Based on Binary Frequency-Shift Keying Modulation: A New Step toward Improved Noise Resilience.

Visible light communications (VLC) are an emerging technology that is increasingly demonstrating its ability to provide wireless communications in areas where radio frequency (RF) technology might have some limitations. Therefore, VLC systems offer possible answers to various applications in outdoor conditions, such as in the road traffic safety domain, or even inside large buildings, such as in indoor positioning applications for blind people. Nevertheless, several challenges must still be addressed in order to obtain a fully reliable solution. One of the most important challenges is focused on further improving the immunity to optical noise. Different from most works, where on-off keying (OOK) modulation and Manchester coding have been the preferred choices, this article proposes a prototype based on a binary frequency-shift keying (BFSK) modulation and non-return-to-zero (NRZ) coding, for which the resilience to noise is compared to that of a standard OOK VLC system. The experimental results showed an optical noise resilience improvement of 25% in direct exposure to incandescent light sources. The VLC system using BFSK modulation was able to maintain a maximum noise irradiance of 3500 µW/cm2 as compared with 2800 µW/cm2 for the OOK modulation, and an improvement of almost 20% in indirect exposure to the incandescent light sources. The VLC system with BFSK modulation was able to maintain the active link in an equivalent maximum noise irradiance of 65,000 µW/cm2, as opposed to the equivalent 54,000 µW/cm2 for the OOK modulation. Based on these results, one can see that based on a proper system design, VLC systems are able to provide impressive resilience to optical noise.

Frequency Modulation Approach for High Power Density 100 Hz Piezoelectric Vibration Energy Harvester.

Piezoelectric vibration energy harvester (PVEH) is a promising device for sustainable power supply of wireless sensor nodes (WSNs). PVEH is resonant and generates power under constant frequency vibration excitation of mechanical equipment. However, it cannot output high power through off-resonance if it has frequency offset in manufacturing, assembly and use. To address this issue, this paper designs and optimizes a PVEH to harvest power specifically from grid transformer vibration at 100 Hz with high power density of 5.28 µWmm-3g-2. Some resonant frequency modulation methods of PVEH are discussed by theoretical analysis and experiment, such as load impedance, additional mass, glue filling, axial and transverse magnetic force frequency modulation. Finally, efficient energy harvesting of 6.1 V output in 0.0226 g acceleration is tested in grid transformer reactor field application. This research has practical value for the design and optimization process of tunable PVEH for a specific vibration source.

The Influence of Temperature on Frequency Modulation Spectroscopy in Atom Gravimeter.

Atom gravimeters use locked lasers to manipulate atoms to achieve high-precision gravity measurements. Frequency modulation spectroscopy (FMS) is an accurate method of optical heterodyne spectroscopy, capable of the sensitive and rapid frequency locking of the laser. Because of the effective absorption coefficient, Doppler broadening and susceptibility depend on temperature, and the signal-to-noise ratio (SNR) of the spectroscopy could be affected by temperature. We present a detailed study of the influence of the temperature on FMS in atom gravimeters, and the experimental results show that the SNR of the spectroscopy is dependent on temperature. In this paper, the frequency of the reference laser is locked by tracking the set point of the fringe slope of FMS. The influence of the frequency-locking noise of the reference laser on the sensitivity of the atom gravimeter is investigated by changing the temperature of the Rb cell without extra operations. The method presented here could be useful for improving the sensitivity of quantum sensors that require laser spectroscopic techniques.

Peculiar Atomic Bond Nature in Platinum Monatomic Chains.

Metal atomic chains have been reported to change their electronic or magnetic properties by slight mechanical stimulus. However, the mechanical response has been veiled because of lack of information on the bond nature. Here, we clarify the bond nature in platinum (Pt) monatomic chains by our in situ transmission electron microscope method. The stiffness is measured with sub-N/m precision by quartz length-extension resonator. The bond stiffnesses at the middle of the chain and at the connection to the base are estimated to be 25 and 23 N/m, respectively, which are higher than the bulk counterpart. Interestingly, the bond length of 0.25 nm is found to be elastically stretched to 0.31 nm, corresponding to a 24% strain. Such peculiar bond nature could be explained by a novel concept of "string tension". This study is a milestone that will significantly change the way we think about atomic bonds in one-dimension.

Long-Range Detection of a Wirelessly Powered Resistive Transducer.

Wireless measurement of resistance variation is particularly desirable inside confined cavities where wire connection and battery replacement are undesirable. Compared to capacitive or inductive transducers, resistive transducers have better availability, whose resistance changes can be directly converted into detectable voltages by electric bridges. However, to wirelessly operate electric bridges on batteryless platforms, multistage circuits are required to convert dc signals into wireless signals, making the whole system hard to miniaturize without using complicated integrated circuits. Alternatively, resistive transducers can be incorporated into passive L C resonators for contactless characterization by the backscattering method. This design, however, is ineffective beyond the near field, and it requires complicated line shape analysis of resonators' frequency response curves. Here, we will significantly improve the remote detectability of a resistive transducer, by inductively coupling it with a parametric resonator. Upon activation by wireless pumping power with an external antenna, the parametric resonator can self-oscillate and emit strong oscillation signals. The temperature-induced resistance change is converted into linear frequency shifts of the oscillation signal that can be detected over large distance separations for up to 20-fold the sensor's own dimension. Every 0.1 °C of temperature change can be converted into 8 kHz of frequency shift that is approximately threefold larger than the linewidth of oscillation peak. This sensor maintains good linearity between 25 °C and 41 °C, providing enough range for physiological monitoring. In conclusion, we have fabricated a resistance-to-frequency converter for remote detection of resistance changes via a wirelessly powered parametric oscillator. Besides this proof-of-concept demonstration for temperature sensing, the general concept of resistance-to-frequency conversion will improve the remote detectability of a broad range of resistive transducers for physiological and environmental monitoring.

The plant hormone auxin beats the time for oscillating light-regulated lateral root induction.

The molecular mechanism underlying the periodic induction of lateral roots, a paradigmatic example of clock-driven organ formation in plant development, is a matter of ongoing, controversial debate. Here, we provide experimental evidence that this clock is frequency modulated by light and that auxin serves as a mediator for translating continuous light signals into discontinuous gene activation signals preceding the initiation of lateral roots in Arabidopsis seedlings. Based on this evidence, we propose a molecular model of an ultradian biological clock involving auxin-dependent degradation of an AUX/IAA-type transcription repressor as a flexible, frequency-controlling delay element. This model widens the bandwidth of biological clocks by adding a new type that allows the pace of organ formation to adapt to the changing environmental demands of the growing plant.

Rapid frequency modulation in a resonant system: aerial perturbation recovery in hawkmoths.

Centimetre-scale fliers must contend with the high power requirements of flapping flight. Insects have elastic elements in their thoraxes which may reduce the inertial costs of their flapping wings. Matching wingbeat frequency to a mechanical resonance can be energetically favourable, but also poses control challenges. Many insects use frequency modulation on long timescales, but wingstroke-to-wingstroke modulation of wingbeat frequencies in a resonant spring-wing system is potentially costly because muscles must work against the elastic flight system. Nonetheless, rapid frequency and amplitude modulation may be a useful control modality. The hawkmoth Manduca sexta has an elastic thorax capable of storing and returning significant energy. However, its nervous system also has the potential to modulate the driving frequency of flapping because its flight muscles are synchronous. We tested whether hovering hawkmoths rapidly alter frequency during perturbations with vortex rings. We observed both frequency modulation (32% around mean) and amplitude modulation (37%) occurring over several wingstrokes. Instantaneous phase analysis of wing kinematics revealed that more than 85% of perturbation responses required active changes in neurogenic driving frequency. Unlike their robotic counterparts that abdicate frequency modulation for energy efficiency, synchronous insects use wingstroke-to-wingstroke frequency modulation despite the power demands required for deviating from resonance.

Adaptive stochastic resonance in bistable system driven by noisy NLFM signal: phenomenon and application.

The stochastic resonance (SR) in a bistable system driven by nonlinear frequency modulation (NLFM) signal and strong noise is studied. Combined with empirical mode decomposition (EMD) and piecewise idea, an adaptive piecewise re-scaled SR method based on the optimal intrinsic mode function (IMF), is proposed to enhance the weak NLFM signal. At first, considering the advantages of EMD for dealing with non-stationary signals, the segmented NLFM signal is processed by EMD. Meanwhile, the cross-correlation coefficient is used as the measure to select the optimal IMF that contains the NLFM signal feature. Then, the spectral amplification gain indicator is proposed to realize the adaptive SR of the optimal IMF of each sub-segment signal and reconstruct the enhanced NLFM signal. Finally, the effectiveness of the proposed method is highlighted with the analysis of the short-time Fourier transform spectrum of the simulation results. As an application example, the proposed method is verified adaptability in bearing fault diagnosis under the speed-varying condition that represents a typical and complicated NLFM signal in mechanical engineering. The research provides a new way for the enhancement of weak non-stationary signals. This article is part of the theme issue 'Vibrational and stochastic resonance in driven nonlinear systems (part 1)'.