RESUMEN
Anthocyanin is a natural plant pigment that acts as an antioxidant and scavenges free radicals. This study aimed to investigate the potential protective role of nightshade anthocyanin (NA), a natural flavonoid compound, against the arsanilic acid (ASA)-induced cell death of DF-1 cells. DF-1 cells were initially exposed to ASA, and then NA was applied to the treated cells. Cell viability, intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and apoptosis were examined. Results showed that NA inhibited the ASA-induced decrease in cell viability, increase in ROS, and loss of MMP in DF-1 cells. Moreover, caspase-3 activation was inhibited by ASA supplementation and NA attenuated the ASA-induced increase in the percentage of apoptotic cells. In summary, our study suggested that NA can enhance ASA-induced cytotoxicity and apoptosis, thereby providing a basis for the molecular mechanisms of NA-mediated protection.
Asunto(s)
Antocianinas/farmacología , Animales , Antocianinas/metabolismo , Antioxidantes/farmacología , Apoptosis/efectos de los fármacos , Ácido Arsanílico/efectos adversos , Ácido Arsanílico/metabolismo , Caspasa 3/efectos de los fármacos , Línea Celular , Supervivencia Celular/efectos de los fármacos , Embrión de Pollo , Flavonoides/farmacología , Peróxido de Hidrógeno/farmacología , Potencial de la Membrana Mitocondrial/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismoAsunto(s)
Ácido Arsanílico/efectos adversos , Arsenicales/efectos adversos , Ácidos Bóricos/efectos adversos , Dermatitis por Contacto/etiología , Sulfanilamidas/efectos adversos , Ácido Arsanílico/análogos & derivados , Dermatitis por Contacto/diagnóstico , Combinación de Medicamentos/efectos adversos , Femenino , Humanos , Persona de Mediana Edad , Pruebas del ParcheRESUMEN
The effects of arsanilic acid as promoter of faster growth on the nutrition index and intestinal absorption of galactose and arabinose has been studied in growing rats. The rats that received arsanilic acid 0.7 mg/day added to water showed gain in body weight and feed intake, I.T. (feed conversion ratio) decrease and P.E.R. (protein efficiency ratio) increase. A dose of 1.4 mg/day produced harmful effects. The in vitro intestinal transport of galactose and arabinose decreased and increased respectively in animals that received additive in comparison with controls.
Asunto(s)
Arabinosa/metabolismo , Ácido Arsanílico/farmacología , Arsenicales/farmacología , Peso Corporal/efectos de los fármacos , Galactosa/metabolismo , Absorción Intestinal/efectos de los fármacos , Alimentación Animal/análisis , Animales , Ácido Arsanílico/administración & dosificación , Ácido Arsanílico/efectos adversos , Transporte Biológico Activo/efectos de los fármacos , Ratas , Ratas Endogámicas/crecimiento & desarrolloRESUMEN
Bilateral vestibular dysfunction was induced in Long-Evans male rats (n = 7) by intratympanic injections of sodium arsanilate (30 mg/side). Control rats (n = 6) received isotonic saline. Animals were tested for labyrinthine integrity by measuring air-righting and contact-righting reflexes. Rats were reduced to 85% of free-feeding body weight and tested in an enclosed 8-arm radial maze (1 trial/day over 10 days). Labyrinthectomized animals made significantly more errors (p < .001) and, unlike the controls, showed no significant improvement on this measure over acquisition training. These rats also made significantly more (p = 0.018) sequential same arm reentries and fewer sequential adjacent arm entries (p < .01). These findings demonstrate that information obtained from the vestibular system is very important in spatial learning in the rat.