The effects of dietary non-starch polysaccharides on Ascaridia galli infection in grower layers.

This study examined whether Ascaridia galli infection can be controlled by dietary non-starch polysaccharides (NSP) in chickens. One-day-old chicks were fed either a basal diet (CON) or CON plus insoluble NSP (I-NSP), or CON plus soluble NSP (S-NSP) for 11 weeks. Three weeks later, birds from half of each feeding group were inoculated with 250 embryonated eggs of A. galli, and slaughtered 8 weeks post-infection to determine worm counts. Both NSP diets, particularly S-NSP, increased prevalence of infection (P<0·05) and worm burden (roughly +50%) of the birds (P<0·001). A. galli infection caused a less efficient (P=0·013) feed utilization for body weight gain (BWG) resulting in lower body weights (P<0·001) irrespective of type of diet consumed. NSP-fed birds, particularly those on I-NSP, consumed more (+8%) feed per unit BWG and showed retarded (P<0·001) BW development compared to CON-fed birds. Intracaecal pH was lowered by S-NSP (P<0·05). Both NSP diets increased the volatile fatty acids pool size in caeca (P<0·001) with S-NSP exerting a greater effect (+46%) than I-NSP (+24%). It is concluded that both NSPs supplemented diets alter gastrointestinal environment in favour of the nematode establishment, and thus have no potential for controlling A. galli infection in chickens.

Identification of Ascaridia numidae in guinea fowl (Numida meleagris) and association with elevated mortality.

An outbreak of ascaridiasis occurred in 10-wk-old guinea fowl (Numida meleagris) on a commercial farm. Birds had exhibited elevated mortality (11.66%) in the previous week, as well as increased water consumption, weakness, anorexia, and stunted growth. Numerous nematodes, occasionally occluding the intestinal lumen, were present in the jejunum and ileum and were identified as Ascaridia numidae based on microscopic morphology. Ribosomal DNA 18S and 28S D3 sequences of the nematode were deposited into GenBank and found to be most similar to Ascaridia galli and Toxocara vitulorum, respectively; sequences for A. numidae had not been previously reported. Treatment with piperazine sulfate significantly reduced the number of adult worms in the intestines, greatly decreased eggs per gram of feces, relieved clinical signs in the flock, and returned the flock mortality back to expected levels. All findings implicate A. numidae as the cause of elevated mortality in this flock.

The effect of Plasmodium gallinaceum on a challenge infection with Ascaridia galli in chickens.

The effect of a primary infection with the haemoparasite Plasmodium gallinaceum on the establishment of a challenge infection with the nematode Ascaridia galli in chickens was studied. Four groups were infected as follows. Group 1: inoculated intravenously with 10(6) P. gallinaceum-infected erythrocytes on day 0; group 2: orally infected with 500 embryonated A. galli eggs on day 10; group 3: infected with P. gallinaceum on day 0 and A. galli on day 10; and group 3: non-infected control birds. The results of this investigation demonstrates that a primary infection with P. gallinaceum in chickens alters the course of a subsequent infection with A. galli. Thus, an antagonistic effect was seen in which the malaria infection caused a significant reduction on the establishment of the nematode in concurrently infected animals.

High mortality of domestic turkeys associated with Ascaridia dissimilis.

Third- and fourth-stage Ascaridia dissimilis larvae were isolated from commercial white turkey intestinal scrapings from two farms that were experiencing high mortality. Lesions consisted of a necrotic-like enteritis that was most severe in the jejunum. Subsequent bacteriological isolation yielded heavy growth of Escherichia coli and Clostridium perfringens. The rate of mortality declined rapidly when the turkeys were administered 18 ppm fenbendazole for 7 days.

Diseases of young captive crocodiles in Papua New Guinea.

To identify causes of mortality in young captive crocodiles, detailed necropsy and laboratory examination was done on 54 (30 Crocodylus porosus, 22 C. novaeguineae, 2 of unrecorded species). Although multiple infections often confounded interpretation it was concluded that the major infectious diseases, of approximately equal importance, were coccidiosis, bacterial septicaemia with Gram-negative organisms, and metazoan parasitism including ascariasis and pentastomiasis. A range of other lesions and agents was recognised, including keratitis, enteritis of unknown aetiology, non-suppurative encephalitis, traumatic peritonitis and trematodes located in renal tubules, gut and blood vessels. Some crocodiles in poor condition had only mild lesions associated with metazoan parasites and the cause of death or illness could not be clearly determined, although it was considered likely that adaptation failure was a contributing factor.

Ascariasis in the subdistrict of Cavacos, municipality of Alterosa (MG), Brazil: effect of mass treatment with albendazole on the intensity of infection

Em amostra aleatoria e estratificada da populacao do subdistrito de Cavacos, no municipio de Alterosa (Minas Gerais, Brasil) estudaram-se os aspectos clinicos e epidemiologicos da infeccao por Ascaris lumbricoides. Avaliou-se, tambem, na mesma amostra, seis meses mais tarde, o efeito do tratamento em massa com albendazol sobre a prevalencia e intensidade de infeccao por esse nematoide. Na primeira fase do estudo, realizou-se inquerito em 248 individuos, utilizando questionario que investigava aspectos relativos a condicoes socioeconomicas, sanitarias e clinicas. Foram, tambem, examinadas 230 amostras de fezes pela tecnica de Kato-Katz, visando determinacao da prevalencia e intensidade de infeccao por A. lumbricoides. Ao mesmo tempo, 202 individuos foram submetidos a micro-hematocrito e em 70 criancas com idade menor ou igual a 12 anos efetuou-se avaliacao do estado nutricional. Determinou-se, ainda, a presenca de ovos de A. lumbricoides e outros helmintos em 22 amostras de solo colhidas na zona urbana de Cavacos...