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1.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 41(10): 1016-1023, 2016 Oct 28.
Artículo en Zh | MEDLINE | ID: mdl-27807322

RESUMEN

OBJECTIVE: To observe the effect of high-mobility group box 1 (HMGB1) on autophagy and chemotherapy resistance in human leukemiacell line (K562) cells, and to explore the underlying mechanisms.
 Methods: The K562 cells were cultured in vitro and divided into 6 groups: a chemotherapeutic group, a chemotherapeutic control group, a HMGB1 preconditioning group, a HMGB1 preconditioning control group, a HMGB1 siRNA group and a siRNA control group. The chemotherapeutic group was further divided into a vincristine (VCR) group, an etoposide (VP-16) group, a cytosine arabinoside (Ara-C) group, a adriamycin (ADM) group and a arsenic trioxide (As2O3) group. The cell activity was evaluated by cell counting kit-8. The protein levels of HMGB1, microtubule-associate protein1light chain3 (LC3), AMP-activated protein kinase (AMPK) and mammalian target of rapamycin (m-TOR) were determined by Western blotting. The level of serum HMGB1 was evaluated by enzyme-linked immunosorbent assay (ELISA). The autophagy was examined by monodansylcadaverine staining and observed under transmission electron microscopy.
 Results: Compared with the control group, the cell activity was significantly decreased and the level of serum HMGB1 was significantly increased in the chemotherapeutic (VCR, VP-16, Ara-C, ADM and As2O3) groups (all P<0.05). Compared with the control group, the cell activity and the level of serum HMGB1 were significantly increased in the HMGB1 preconditioning group (both P<0.05). Compared with the siRNA control group, the cell activity and the level of serum HMGB1 were significantly decreased in the HMGB1 siRNA group (both P<0.05). Compared with the control group, the expression of LC3-II and the formation of autophagic bodies were increased in the HMGB1 preconditioning group (both P<0.05), the p-AMPK expression was increased and p-mTOR expression was decreased (both P<0.05).
 Conclusion: HMGB1 can increase the autophagy and promote chemotherapy resistance through the pathway of AMPK/m-TOR in K562 cells.


Asunto(s)
Proteínas Quinasas Activadas por AMP/genética , Proteínas Quinasas Activadas por AMP/fisiología , Autofagia/genética , Resistencia a Antineoplásicos/genética , Resistencia a Antineoplásicos/fisiología , Proteína HMGB1/genética , Proteína HMGB1/fisiología , Serina-Treonina Quinasas TOR/genética , Serina-Treonina Quinasas TOR/fisiología , Trióxido de Arsénico , Arsenicales , Citarabina , Doxorrubicina , Etopósido , Humanos , Células K562/fisiología , Proteínas Asociadas a Microtúbulos , Óxidos , ARN Interferente Pequeño , Transducción de Señal , Vincristina
2.
J Cell Physiol ; 227(5): 2240-51, 2012 May.
Artículo en Inglés | MEDLINE | ID: mdl-21809341

RESUMEN

The goal of the present study is to explore the contribution of tumor necrosis factor-α (TNFα)-related pathway to the cytotoxicity of arecoline on human leukemia K562 cells. Arecoline treatment induced death of K562 cells and increased surface expression of TNFα, TNFR1, and TNFR2. Unlike that of TNFR1 mRNA, transcriptional levels of TNFα and TNFR2 mRNA increased in arecoline-treated cells. Moreover, arecoline-induced down-regulation of ADAM17 maturation was involved in surface up-modulation of TNFR1, TNFR2, and TNFα. Arecoline-elicited increase in intracellular Ca(2+) concentration was responsible for JNK/c-Jun pathway activation and ERK inactivation. Abolition of JNK/c-Jun pathway suppressed arecoline-induced increase in transcriptional level of TNFα and TNFR2 mRNA. TNFα and TNFR2 promoter luciferase activity and chromatin immunoprecipitating analyses revealed that c-Jun increasingly bound with TNFα and TNFR2 promoter upon arecoline treatment. Over-expression of constitutively active MEK1 abolished the effect of arecoline on suppressing ADAM17 maturation. Pretreatment with TNFR2 antibody abrogated arecoline-induced increased susceptibility of K562 cells for the cytotoxicity of TNFα and arecoline-induced cell death. Taken together, our data suggest that up-modulation of TNFR2 surface expression is associated with arecoline-induced death of K562 cells.


Asunto(s)
Arecolina/farmacología , Muerte Celular/efectos de los fármacos , Agonistas Colinérgicos/farmacología , Células K562/efectos de los fármacos , Receptores Tipo II del Factor de Necrosis Tumoral/metabolismo , Regulación hacia Arriba/efectos de los fármacos , Proteínas ADAM/genética , Proteínas ADAM/metabolismo , Antracenos/metabolismo , Calcio/metabolismo , Quelantes/metabolismo , Ácido Egtácico/análogos & derivados , Ácido Egtácico/metabolismo , Quinasas MAP Reguladas por Señal Extracelular/genética , Quinasas MAP Reguladas por Señal Extracelular/metabolismo , Humanos , Proteínas Quinasas JNK Activadas por Mitógenos/genética , Proteínas Quinasas JNK Activadas por Mitógenos/metabolismo , Células K562/fisiología , MAP Quinasa Quinasa 1/genética , MAP Quinasa Quinasa 1/metabolismo , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Sistema de Señalización de MAP Quinasas/fisiología , Interferencia de ARN , Receptores Tipo I de Factores de Necrosis Tumoral/genética , Receptores Tipo I de Factores de Necrosis Tumoral/metabolismo , Receptores Tipo II del Factor de Necrosis Tumoral/genética , Factor de Necrosis Tumoral alfa/genética , Factor de Necrosis Tumoral alfa/metabolismo
3.
Elife ; 102021 12 02.
Artículo en Inglés | MEDLINE | ID: mdl-34854380

RESUMEN

Integrin conformational ensembles contain two low-affinity states, bent-closed and extended-closed, and an active, high-affinity, extended-open state. It is widely thought that integrins must be activated before they bind ligand; however, one model holds that activation follows ligand binding. As ligand-binding kinetics are not only rate limiting for cell adhesion but also have important implications for the mechanism of activation, we measure them here for integrins α4ß1 and α5ß1 and show that the low-affinity states bind substantially faster than the high-affinity state. On- and off-rates are similar for integrins on cell surfaces and as ectodomain fragments. Although the extended-open conformation's on-rate is ~20-fold slower, its off-rate is ~25,000-fold slower, resulting in a large affinity increase. The tighter ligand-binding pocket in the open state may slow its on-rate. Low-affinity integrin states not only bind ligand more rapidly, but are also more populous on the cell surface than high-affinity states. Thus, our results suggest that integrin binding to ligand may precede, rather than follow, activation by 'inside-out signaling.'


Asunto(s)
Sitios de Unión , Adhesión Celular/fisiología , Citoesqueleto/fisiología , Integrinas/química , Células Jurkat/fisiología , Células K562/fisiología , Ligandos , Humanos , Cinética , Modelos Moleculares
4.
J Cell Biol ; 158(4): 787-99, 2002 Aug 19.
Artículo en Inglés | MEDLINE | ID: mdl-12177042

RESUMEN

Leukocytes roll on selectins at nearly constant velocities over a wide range of wall shear stresses. Ligand-coupled microspheres roll faster on selectins and detach quickly as wall shear stress is increased. To examine whether the superior performance of leukocytes reflects molecular features of native ligands or cellular properties that favor selectin-mediated rolling, we coupled structurally defined selectin ligands to microspheres or K562 cells and compared their rolling on P-selectin. Microspheres bearing soluble P-selectin glycoprotein ligand (sPSGL)-1 or 2-glycosulfopeptide (GSP)-6, a GSP modeled after the NH2-terminal P-selectin-binding region of PSGL-1, rolled equivalently but unstably on P-selectin. K562 cells displaying randomly coupled 2-GSP-6 also rolled unstably. In contrast, K562 cells bearing randomly coupled sPSGL-1 or 2-GSP-6 targeted to a membrane-distal region of the presumed glycocalyx rolled more like leukocytes: rolling steps were more uniform and shear resistant, and rolling velocities tended to plateau as wall shear stress was increased. K562 cells treated with paraformaldehyde or methyl-beta-cyclodextrin before ligand coupling were less deformable and rolled unstably like microspheres. Cells treated with cytochalasin D were more deformable, further resisted detachment, and rolled slowly despite increases in wall shear stress. Thus, stable, shear-resistant rolling requires cellular properties that optimize selectin-ligand interactions.


Asunto(s)
Proteínas Portadoras/metabolismo , Movimiento Celular , Glicoproteínas , Células K562/citología , Glicoproteínas de Membrana/metabolismo , Selectina-P/fisiología , Péptidos , Estrés Mecánico , Humanos , Células K562/fisiología , Ligandos , Microesferas , Modelos Biológicos , Mucinas/metabolismo , Células Tumorales Cultivadas
5.
Biotechnol Appl Biochem ; 54(1): 41-52, 2009 Jul 09.
Artículo en Inglés | MEDLINE | ID: mdl-19216718

RESUMEN

Nonsense mutations, giving rise to UAA, UGA and UAG stop codons within the coding region of mRNAs, promote premature translational termination and are the leading cause of approx. 30% of inherited diseases, including cystic fibrosis, Duchenne muscular dystrophy and thalassaemia. For instance, in beta(0)39-thalassaemia the CAG (glutamine) codon is mutated to the UAG stop codon, leading to premature translation termination and to mRNA destabilization through the well-described NMD (nonsense-mediated mRNA decay). In order to develop an approach facilitating translation and, therefore, protection from NMD, aminoglycoside antibiotics have been tested on mRNAs carrying premature stop codons. These drugs decrease the accuracy in the codon-anticodon base-pairing, inducing a ribosomal read-through of the premature termination codons. Interestingly, recent papers have described drugs designed and produced for suppressing premature translational termination, inducing a ribosomal read-through of premature but not normal termination codons. These findings have introduced new hopes for the development of a pharmacological approach to the therapy of beta(0)39-thalassaemia. In this context, we started the development of a cellular model of the beta(0)39-thalassaemia mutation that could be used for the screening of a high number of aminoglycosides and analogous molecules. To this aim, we produced a lentiviral construct containing the beta(0)39-thalassaemia globin gene under a minimal LCR (locus control region) control and used this construct for the transduction of K562 cells, subsequently subcloned, with the purpose to obtain several K562 clones with different integration copies of the construct. These clones were then treated with Geneticin (also known as G418) and other aminoglycosides and the production of beta-globin was analysed by FACS analysis. The results obtained suggest that this experimental system is suitable for the characterization of correction of the beta(0)39-globin mutation causing beta-thalassaemia.


Asunto(s)
Codón sin Sentido , Células K562/fisiología , Mutación Puntual , ARN Mensajero/biosíntesis , Globinas beta/genética , Talasemia beta/genética , Células Clonales , Clonación Molecular , Expresión Génica/efectos de los fármacos , Gentamicinas/farmacología , Proteínas Fluorescentes Verdes/biosíntesis , Proteínas Fluorescentes Verdes/genética , Humanos , Lentivirus/genética , Mutagénesis Sitio-Dirigida , Reacción en Cadena de la Polimerasa , ARN Mensajero/genética , Proteínas Recombinantes de Fusión/biosíntesis , Proteínas Recombinantes de Fusión/genética , Transcripción Genética/efectos de los fármacos , Transfección , Globinas beta/biosíntesis
6.
J Antibiot (Tokyo) ; 72(1): 45-49, 2019 01.
Artículo en Inglés | MEDLINE | ID: mdl-30258222

RESUMEN

Aranciamycin K (1) and isotirandamycin B (2) were isolated from a marine-derived Streptomyces sp. SCSIO 41399, along with the previously reported four anthracycline derivatives (3-6), and two known tirandamycin derivatives (7 and 8). Their structures including absolute configurations were determined by extensive analysis of their spectroscopic analysis and ECD calculation method. Most of the isolated compounds were tested for their cytotoxic, antibacterial, and antifungal activities. Compounds 2, 7 and 8 displayed potent bacteriostatic effects against Streptococcus agalactiae with MIC values of 11.5, 5.9 and 5.7 µM, respectively. Besides, compounds 3, 5 and 6 exhibited moderate in vitro cytotoxic activities against the K562 cell lines with IC50 values of 22.0 ± 0.20, 1.80 ± 0.01 and 12.1 ± 0.07 µM, respectively.


Asunto(s)
Aminoglicósidos/farmacología , Antraciclinas/farmacología , Antibacterianos/farmacología , Antineoplásicos/farmacología , Organismos Acuáticos/química , Streptomyces/química , Aminoglicósidos/química , Aminoglicósidos/aislamiento & purificación , Antraciclinas/química , Antraciclinas/aislamiento & purificación , Antibacterianos/química , Antibacterianos/aislamiento & purificación , Antineoplásicos/química , Antineoplásicos/aislamiento & purificación , Organismos Acuáticos/aislamiento & purificación , Supervivencia Celular/efectos de los fármacos , Humanos , Concentración 50 Inhibidora , Células K562/efectos de los fármacos , Células K562/fisiología , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Análisis Espectral , Streptococcus agalactiae/efectos de los fármacos , Streptomyces/aislamiento & purificación
7.
Sci Rep ; 8(1): 1219, 2018 01 19.
Artículo en Inglés | MEDLINE | ID: mdl-29352174

RESUMEN

Mechanical properties of living cells can be used as reliable markers of their state, such as the presence of a pathological state or their differentiation phase. The mechanical behavior of cells depends on the organization of their cytoskeletal network and the main contribution typically comes from the actomyosin contractile system, in both suspended and adherent cells. In the present study, we investigated the effect of a pharmaceutical formulation (OTC - Ossitetraciclina liquida 20%) used as antibiotic, on the mechanical properties of K562 cells by using the Micropipette Aspiration Technique (MAT). This formulation has been shown to increase in a time dependent way the inflammation and toxicity in terms of apoptosis in in vitro experiments on K562 and other types of cells. Here we show that by measuring the mechanical properties of cells exposed to OTC for different incubation times, it is possible to infer modifications induced by the formulation to the actomyosin contractile system. We emphasize that this system is involved in the first stages of the apoptotic process where an increase of the cortical tension leads to the formation of blebs. We discuss the possible relation between the observed mechanical behavior of cells aspirated inside a micropipette and apoptosis.


Asunto(s)
Células K562/efectos de los fármacos , Células K562/fisiología , Fenómenos Mecánicos , Algoritmos , Células Cultivadas , Humanos , Modelos Teóricos
8.
Tsitologiia ; 49(7): 552-60, 2007.
Artículo en Ruso | MEDLINE | ID: mdl-17918339

RESUMEN

The induction of apoptosis in K562 cells by doxorubicin (DR) was used as a model to investigate changes in the subunit composition, phosphorylation state and enzymatic activities of 26S proteasomes in cells undergoing the programmed death. Here we have shown for the first time that proteasomes isolated from the nuclei of control and induced K562 cells differ in their subunit patterns, as well as in the phosphorylation state of subunits on threonine and tyrosine residues. It has been shown for the first time that trypsin- and chymotrypsin-like, and the endoribonuclease activities of nuclear 26S proteasomes are affected under influence of DR on K562 cells. Treatment of K562 cells with DR leads to modification of zeta/alpha5 and iota/alpha6 proteasomal subunits associated with RNase activity of proteasomes. These findings confirm our hypothesis about so-called reprogramming of nuclear proteasomes population in undergoing apoptosis K562 cells which is manifested by changes in proteasomal composition, phosphorylation state, and enzymatic activities during the programmed cell death.


Asunto(s)
Apoptosis , Núcleo Celular/metabolismo , Complejo de la Endopetidasa Proteasomal/metabolismo , Doxorrubicina/farmacología , Humanos , Células K562/efectos de los fármacos , Células K562/fisiología , Proteínas Nucleares/metabolismo , Fosforilación , Treonina/metabolismo , Tirosina/metabolismo
9.
Tsitologiia ; 49(6): 451-9, 2007.
Artículo en Ruso | MEDLINE | ID: mdl-17802742

RESUMEN

Here we have studied changes in the subunit composition, phosphorylation state and enzymatic activities of 26S proteasomes in cells undergoing the programmed cell death. Apoptosis in proerythroleukemic K562 cells was induced by glutathione-depleting agent, diethylmaleate (DEM). We have shown for the first time that proteasomes isolated from the nuclei of control and induces K562 cells differ in their subunit patterns, as well as in the phosphorylation state of subunits on threonine and tyrosine residues. We observed trypsin- and chymotrypsin-like activities on nuclear proteasomes and the specificity of proteasomal nucleolysis of several individual messenger RNAs (c-fos and c-myc) to be changed under effect of DEM on K562 cells. Treatment of K562 cells with DEM leads to modification of zeta/alpha5 and iota/alpha6 proteasomal subunits associated with RNAse activity of proteasomes. These findings confirm our hypothesis about so-called reprogramming of nuclear proteasome population in undergoing apoptosis K562 cells which is manifested by the changes in proteasomal composition, phosphorylation state, and enzymatic activities during the programmed cell death.


Asunto(s)
Apoptosis , Maleatos/farmacología , Complejo de la Endopetidasa Proteasomal/metabolismo , Núcleo Celular/metabolismo , Glutatión/efectos de los fármacos , Glutatión/metabolismo , Humanos , Células K562/efectos de los fármacos , Células K562/fisiología , Peso Molecular , Proteínas Nucleares/química , Proteínas Nucleares/metabolismo , Fosforilación , Complejo de la Endopetidasa Proteasomal/química , Subunidades de Proteína/metabolismo , Ribonucleasas/metabolismo
10.
Tsitologiia ; 48(2): 133-41, 2006.
Artículo en Ruso | MEDLINE | ID: mdl-16737180

RESUMEN

The participation of proteasome in the programmed cells death is now extensively investigated. Studies using selective inhibitors of proteasomes have provided a direct evidence of both pro- and anti-apoptotic functions of proteasomes. Such opposite roles of 26S proteasomes in regulation of apoptosis may be defined by the proliferative state of cell. The induction of apoptosis in K562 cells by diethylmaleate was used as a model to investigate changes in the subunit composition, phosphorylation state and enzymatic activities of 26S proteasomes undergoing the programmed cell death. Here we have shown that proteasomes isolated from the cytoplasm of control and diethylmaleate treated K562 cells differ in their subunit patterns, as well as in the phosphorylation state of subunits on threonine and tyrosine residues. It has been shown for the first time that proteolytic activity of 26S proteasomes is decreased, and endoribonuclease activity of 26S proteasomes is affected under diethylmaleate action on K562 cells. Treatment of K562 cells with an inductor of apoptosis--diethylmaleate--leads to modification of a proteasomal subunit (zeta/alpha5) associated with RNase activity of proteasomes. These data suggest the subunit composition and enzymatic activities of 26S proteasomes to be changed in K562 cells undergoing apoptosis, and that specific subtypes of 26S proteasomes participate in execution of programmed death of these cells.


Asunto(s)
Apoptosis , Complejo de la Endopetidasa Proteasomal/metabolismo , Humanos , Células K562/efectos de los fármacos , Células K562/metabolismo , Células K562/fisiología , Maleatos/farmacología , Peso Molecular , Fosforilación , Complejo de la Endopetidasa Proteasomal/química , Ribonucleasas/metabolismo , Treonina , Tirosina
11.
Cancer Res ; 60(23): 6585-9, 2000 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-11118038

RESUMEN

Among the different cellular factors that regulated p53 functions, we previously identified (P. Drane et al., Oncogene, 15: 3013-3024, 1997) RB18A, a new gene whose encoded Mr 205,000 protein interacted in vitro, through its COOH-terminal domain, with p53. Therefore, we analyzed the in vivo role of RB18A by measuring its effect on the transactivating activity of p53 on physiological promoters. We herein demonstrated that RB18A, which interacted also in vivo with p53, activated Bax promoter and inhibited p21Waf1 or IGF-BP3 promoters. In addition, fluorescence in situ hybridization mapping led to localizing the RB18A gene on chromosome 17q12-q21.1, loci associated with human cancers. This is the first demonstration that in vivo RB18A, in a protein-protein interaction, regulates p53 transactivating activity.


Asunto(s)
Proteínas Portadoras/fisiología , Cromosomas Humanos Par 17 , Proteínas Proto-Oncogénicas c-bcl-2 , Factores de Transcripción , Activación Transcripcional/fisiología , Proteína p53 Supresora de Tumor/fisiología , Proteínas Portadoras/genética , Proteínas Portadoras/metabolismo , Inhibidor p21 de las Quinasas Dependientes de la Ciclina , Ciclinas/genética , Humanos , Proteína 3 de Unión a Factor de Crecimiento Similar a la Insulina/genética , Células K562/metabolismo , Células K562/fisiología , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/metabolismo , Subunidad 1 del Complejo Mediador , Regiones Promotoras Genéticas , Proteínas Proto-Oncogénicas/genética , Proteína p53 Supresora de Tumor/genética , Proteína p53 Supresora de Tumor/metabolismo , Proteína X Asociada a bcl-2
12.
Ann Clin Lab Sci ; 45(6): 674-9, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26663798

RESUMEN

In the case of blood type B3 with typical mixed-field agglutination of RBCs in the presence of anti-B or anti-AB antibody, a number of genetic alternations have been reported. It is well known that the IVS3+5G→A mutation in the B gene destroys the consensus of the splice donor site leading to exon 3 skipping during mRNA splicing. The lack of exon 3 likely causes a short stem region, producing an unstable B3 protein, and is concomitant with a decrease in B3 protein expression. Whether the phenomenon also appears in the type A blood group is of question. In this study, we evaluate whether exon 3 deletion in the blood type A gene also results in mixed-field phenotype. Site-directed mutagenesis was used to generate cDNA encoding A1 gene with exon 3 deletion. The cDNA was stably expressed in K562 cells. The expression of A antigen was compared with expression in parental K562 cells that did not express A antigen and in the stable K562 cell line expressing A(1) cDNA by flow cytometry analyses. The expression of A antigen in A1 stable cells and parental K562 cells was set as 100% and 0%, respectively. The mean relative percentage of A antigen expression for the cells of A1 with exon 3 deletion was 59.9% of A1 stable cells. Consistent with the observations of B3, which is B gene with exon 3 deletion, mixed field agglutination was observed for the cells expressing A1 with exon 3 deletion. Exon 3 deletion results in mixed field phenotype in both type A and B RBCs. However, the degree of antigen expression change for exon 3 deletion in A gene was less severe when compared with the deletion occurred in B gene.


Asunto(s)
Sistema del Grupo Sanguíneo ABO/genética , Células K562/fisiología , Sistema del Grupo Sanguíneo ABO/metabolismo , Aglutinación , Alelos , Exones , Humanos , Eliminación de Secuencia
13.
J Gerontol A Biol Sci Med Sci ; 53(6): B430-7, 1998 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-9823739

RESUMEN

We investigated the effects of a graded maximal exercise treadmill test on natural killer (NK) cell number, activity, and responsiveness to interferon-alpha (IFN-alpha) in young (22+/-0.7 yrs) and elderly (65+/-0.8 yrs) sedentary subjects. NK cell cytotoxicity (NKCC) was determined using Ficoll purified peripheral blood mononuclear cells (PBMCs) by a 51Cr release assay against NK-sensitive (K562) and NK-insensitive (Daudi) target cells at various effector:target (E:T) ratios before and immediately after exercise. PBMCs were incubated with rhu IFN-alpha (125 and 250u/10(6) PBMCs) or without for 2 hrs before addition to the 51Cr release assay. There were no differences in unstimulated NKCC against K562 or Daudi targets between the old and the young despite significantly (p=.01) higher percentages of CD56+ NK cells (21.1+/-2.3% in old vs 12.5+/-2.5% in young, pre-exercise). IFN-alpha increased NKCC versus both targets, and NK cells from old subjects were hyporesponsive to IFN-alpha stimulation; this was especially evident at low E:T ratios versus Daudi cells. Maximal exercise significantly increased (50-200%) unstimulated NKCC versus K562 and Daudi targets similarly in both young and old and increased the percentage of CD56+ cells in the PBMC fraction to 33.3+/-3.7% and 23.3+/-3.6% in old and young, respectively. We found a significant correlation between %CD56+ and basal NKCC versus K562s and Daudi cells in the young (i.e., r=.55; p=.02 vs K562s), but not the old (i.e., r=.20; p=.29 vs K562s) subjects. This indicates that, in the young, part of the exercise-induced increase in NKCC is due to an increase in NK cell number. Maximal exercise did not affect unstimulated per cell killing of K562s, but tended to increase per cell killing of Daudis. These results indicate that CD56+ cells from old subjects have an intrinsic defect in their ability to perform cytolysis and respond to IFN-alpha. Furthermore, a single bout of maximal exercise increases NKCC and CD56+ cell number similarly in both young and old subjects regardless of the target cell used.


Asunto(s)
Envejecimiento/fisiología , Citotoxicidad Inmunológica/efectos de los fármacos , Citotoxicidad Inmunológica/fisiología , Ejercicio Físico , Interferón-alfa/farmacología , Células Asesinas Naturales/efectos de los fármacos , Células Asesinas Naturales/fisiología , Adolescente , Adulto , Anciano , Actividad Bactericida de la Sangre/fisiología , Antígeno CD56/análisis , Femenino , Humanos , Células K562/fisiología , Células Asesinas Naturales/inmunología , Recuento de Leucocitos/efectos de los fármacos , Masculino , Persona de Mediana Edad , Resistencia Física , Proteínas Recombinantes
14.
Anticancer Res ; 22(6C): 3827-32, 2002.
Artículo en Inglés | MEDLINE | ID: mdl-12553001

RESUMEN

BACKGROUND: Anticancer agents modulate gene expression and these changes are essential for tumor cell killing. To investigate the mechanism by which etoposide acts as an anticancer agent, the relationship between p21WAF1/CIP1 (p21) and c-Myc was studied. MATERIALS AND METHODS: K562 cells with and without ectopic c-Myc expression were studied. Apoptosis was detected using propidium iodide and Hoechst 33342 double staining. The c-Myc and p21 levels were studied by RT-PCR and immunoblot. The p21 promoter (from -205 to +67) was investigated by the luciferase reporter gene assay. RESULTS: Ectopic c-Myc-expressing K562 (K562/c-Myc) cells showed more extensive apoptosis than K562 cells after continuous exposure to 200 microM etoposide for 24 hours. During this treatment, p21 expression was not observed in K562/c-Myc cells, and the expression of c-Myc and p21 was mutually exclusive. Etoposide activated the p21 promoter in a concentration-dependent manner, and etoposide-induced luciferase activity was suppressed by co-transfection of c-Myc. CONCLUSION: p21 promoter activity was repressed by c-Myc in proliferating K562 cells, and detoposide-induced down-regulation of c-Myc released this suppression, resulting in the induction of p21.


Asunto(s)
Antineoplásicos Fitogénicos/farmacología , Ciclinas/biosíntesis , Inhibidores Enzimáticos/farmacología , Etopósido/farmacología , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Células K562/efectos de los fármacos , Proteínas Proto-Oncogénicas c-myc/biosíntesis , Inhibidores de Topoisomerasa II , Apoptosis/efectos de los fármacos , Secuencia de Bases , Inhibidor p21 de las Quinasas Dependientes de la Ciclina , Ciclinas/antagonistas & inhibidores , Ciclinas/genética , Regulación hacia Abajo/efectos de los fármacos , Genes myc/efectos de los fármacos , Genes myc/genética , Humanos , Células K562/metabolismo , Células K562/fisiología , Datos de Secuencia Molecular , Regiones Promotoras Genéticas , Proteínas Proto-Oncogénicas c-myc/genética , Transcripción Genética/efectos de los fármacos
15.
FEBS Lett ; 585(14): 2235-42, 2011 Jul 21.
Artículo en Inglés | MEDLINE | ID: mdl-21658388

RESUMEN

The aim of this study was to investigate the inhibitory mechanism of pure curcumin on WT1 expression in leukemic K562 cells. Pure curcumin suppressed WT1 expression, independent of effects on protein degradation or WT1 mRNA stability. Chromatin immunoprecipitation and reporter gene assays indicate that pure curcumin treatment attenuates WT1 auto-regulation. Interestingly, PKCα inhibition mimicks the repressive effects of pure curcumin in K562 cells. Conversely, myristoylated PKCα over-expression increased WT1 expression and reversed the inhibitory effect of pure curcumin. Our study indicates that pure curcumin attenuates WT1 auto-regulatory function through inhibition of PKCα signaling in K562 cells.


Asunto(s)
Antineoplásicos/farmacología , Curcumina/farmacología , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Células K562 , Proteína Quinasa C-alfa/metabolismo , Transducción de Señal/efectos de los fármacos , Proteínas WT1/genética , Genes Reporteros , Humanos , Células K562/efectos de los fármacos , Células K562/fisiología , Regiones Promotoras Genéticas , Proteínas WT1/metabolismo
16.
Environ Toxicol Pharmacol ; 32(2): 285-95, 2011 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-21843810

RESUMEN

Benzene is a well-known environmental pollutant that can induce hematotoxicity, aplastic anemia, acute myelogenous leukemia, and lymphoma. Benzene toxicity is likely mediated through metabolites induced by means of multiple pathways. Although benzene metabolites are known to induce oxidative stress and disrupt the cell cycle, the mechanism underlying leukemogenesis is not fully understood. The aim of this study was to analyze the genome-wide expression profiles of human promyelocytic leukemia HL-60 cells that had been exposed to benzene and its metabolites. This was carried out using whole human genome oligonucleotide microarrays to ascertain potential biomarkers. Genes that were differentially expressed (>1.5-fold and p-values <0.05) after exposure to benzene (BZ), hydroquinone (HQ), and 1,4-benzoquinone (BQ) were then classified with GO, KEGG and GSEA pathway annotation. All genes that were identified were then functionally categorized as being involved in the cell cycle, the p53 signaling pathway, apoptosis, the MAPK signaling pathway, or the T cell receptor signaling pathway. Functionally important genes were further validated by means of real-time RT-PCR. The results showed that EGR1, PMAIP1, AR, CCL2, CD69, HSPA8, SLC7A11, HERPUD1, ELK1, and MKI57 genes altered their expression profiles. Similar expression profiles were also found in human erythromyeloblastoid leukemia K562 cells and in human leukemic monocyte lymphoma U937 cells. In conclusion, gene expression profiles along with GO, KEGG and GSEA pathway annotation analysis have provided an insight into the leukemogenesis as well as highlighted potential gene-based biomarkers of human leukemia cell lines when they are exposed to benzene and its metabolites.


Asunto(s)
Benceno/toxicidad , Benzoquinonas/toxicidad , Expresión Génica/efectos de los fármacos , Células HL-60/efectos de los fármacos , Hidroquinonas/toxicidad , Células K562/efectos de los fármacos , Células U937/efectos de los fármacos , Benceno/metabolismo , Benzoquinonas/metabolismo , Biomarcadores/metabolismo , Análisis por Conglomerados , Contaminantes Ambientales/metabolismo , Contaminantes Ambientales/toxicidad , Perfilación de la Expresión Génica , Células HL-60/fisiología , Humanos , Hidroquinonas/metabolismo , Células K562/fisiología , Leucemia/genética , Análisis por Micromatrices , Transducción de Señal/genética , Células U937/fisiología
17.
Artículo en Inglés | MEDLINE | ID: mdl-21293542

RESUMEN

AIM: The study was carried out to evaluate the effect of several substrates on oxidative stress induced apoptosis and in K-562 cells. METHODS: Glucose at 5, 11 and 30 mM concentrations was tested, as well as 5 mM glutamine and 5 mM fructose. The cells were exposed to tert-butylhydroperoxide (tBH) and apoptotic cells were evaluated by flow cytometry with FITC-Annexin V and propidium iodide. The effect of glucose concentration on DNA damage was evaluated using hydrogen peroxide and electrophoretic "DNA comets" assay at 5 mM and 30 mM glucose concentrations. RESULTS: The exposure of cells to tBH resulted in increased number of apoptotic cells, and this effect was prevented by administration of an antioxidant - N-Acetyl cysteine. Rising concentrations of glucose added to the toxic effect of tBH; we also observed some toxic effect of fructose and no effect of glutamine. We found higher susceptibility to hydrogen peroxide induced DNA damage with 30 mM glucose concentration. CONCLUSION: Hyperglycemia increases the cell's susceptibility to oxidative stress and it also amplifies oxidative DNA damage. Glutamine - when used as a sole energetic substrate - showed no protective effect against oxidative stress.


Asunto(s)
Apoptosis , Daño del ADN , Glucosa/administración & dosificación , Células K562/efectos de los fármacos , Células K562/fisiología , Estrés Oxidativo , Acetilcisteína/farmacología , Antioxidantes/farmacología , Relación Dosis-Respuesta a Droga , Sinergismo Farmacológico , Fructosa/farmacología , Glutamina/farmacología , Humanos , Peróxido de Hidrógeno/farmacología , terc-Butilhidroperóxido/farmacología
18.
OMICS ; 13(6): 467-76, 2009 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-20001861

RESUMEN

Multidrug resistance (MDR) to chemotherapy is a significant barrier to the effective treatment of chromic myeloid leukemia (CML). In an attempt to identify more factors associated with MDR for an understanding of the mechanism, we first established an adriamycin (ADR)-resistant human erythroleukemia cell line K562/ADR by stepwise selection in vitro using ADR. Besides the elevated resistance to ADR, the K562/ADR cells also showed significantly increased crossed-resistance to vincristin and Gleevec, compared to the parental K562 cells. Then we compared the global protein profiles between K562 and K562/ADR cells. Following two-dimensional gel electrophoresis and image analysis, some of the proteins with different levels between the two cell lines were identified by MALDI TOF/TOF mass spectrometry and Western blot analysis. The differentially expressed proteins were classified into groups based on their functions: calcium-binding proteins, chaperones, metabolic enzymes, proteins related to protein synthesis or DNA synthesis, and proteins related to signal transduction. In particular, ANXA1, a protein that was downregulated in K562/ADR, was analyzed further for its involvement in MDR by transfection and subsequent assays. The functional validation showed that the downregulated ANXA1 expression contributes considerably to the observed drug resistance in K562/ADR cells. These data will be valuable for further study of the mechanisms of MDR and may reveal a potential new diagnostic marker to chemotherapy.


Asunto(s)
Anexina A1/metabolismo , Doxorrubicina/farmacología , Resistencia a Múltiples Medicamentos/fisiología , Células K562 , Proteómica/métodos , Miembro 1 de la Subfamilia B de Casetes de Unión a ATP/metabolismo , Animales , Anexina A1/genética , Proteínas de Unión al Calcio/metabolismo , Doxorrubicina/uso terapéutico , Humanos , Células K562/efectos de los fármacos , Células K562/fisiología , Leucemia Eritroblástica Aguda/tratamiento farmacológico , Leucemia Mielógena Crónica BCR-ABL Positiva/tratamiento farmacológico , Datos de Secuencia Molecular , Análisis por Matrices de Proteínas , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
19.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 24(12): 1167-9, 2008 Dec.
Artículo en Zh | MEDLINE | ID: mdl-19068202

RESUMEN

AIM: To obtain sufficient numbers of pure and activated human primary NK cells for potential clinical application. METHODS: PBMC based ex vivo expansion of natural killer cells was set up. The expansion was initiated by co-culture of PBMC and stimulator cells (irradiate genetic modified K562 cells) in RPMI1640 medium with 1 000 U/mL IL-2. Genetic modified K562 cells 'K562D3' were prepared by expressing IL-15, 4-1BBL and IL-18 on K562 cell membrane. RESULTS: An average of 500 fold expansion of CD56(+)CD3(-) cells was observed after 3 weeks of co-culture. The NK cells population could reach 93% after expansion, comparing with 7% before expansion. The expanded NK cells lysed 95% of K562 targets in a 5:1 effector to target ratio. IL-15/4-1BBL bound K562 stimulatory cells could expand same fold NK cells as K562D3, but the cytotoxicity of NK cells expanded by 'K562D3' was 10% higher. CONCLUSION: The described method is a simple and efficient way for the expansion of human NK cells.


Asunto(s)
Técnicas de Cultivo de Célula/métodos , Células Asesinas Naturales/citología , Células Asesinas Naturales/inmunología , Ligando 4-1BB/genética , Ligando 4-1BB/metabolismo , Adulto , Células Cultivadas , Femenino , Citometría de Flujo , Humanos , Interleucina-15/genética , Interleucina-15/metabolismo , Interleucina-18/genética , Interleucina-18/metabolismo , Interleucina-2/farmacología , Células K562/metabolismo , Células K562/fisiología , Células Asesinas Naturales/efectos de los fármacos , Células Asesinas Naturales/metabolismo , Leucocitos Mononucleares/citología , Leucocitos Mononucleares/efectos de los fármacos , Masculino , Persona de Mediana Edad , Adulto Joven
20.
Br J Haematol ; 137(3): 252-61, 2007 May.
Artículo en Inglés | MEDLINE | ID: mdl-17408467

RESUMEN

Iron is required for the differentiation of HL-60 cells along the monocyte lineage in vitro, reflecting a requirement for iron in the transcriptional induction of the p21(WAF1/CIP1) gene. To determine if the same requirement holds true for differentiation in other cell lineages and for primary human CD34(+) bone marrow precursor cells, we induced granulocyte differentiation by treating HL-60 cells with dimethyl sulphoxide, and erythroid or megakaryocytic differentiation by treating K562 cells with butyrate or phorbol myristate acetate, respectively. Nitro blue tetrazolium reduction, expression of haem, or expression of CD41 was used to assess granulocytic, erythroid, or megakaryocytic differentiation respectively. Purified CD34(+) cells were cultured with granulocyte/macrophage-colony stimulating factor and stem cell factor to induce myelomonocytic differentiation. Iron deprivation was induced by desferrioxamine. p21(WAF1/CIP1) antisense oligonucleotides were used to inhibit p21 expression. Iron deprivation blocked p21 induction as judged by real-time polymerase chain reaction assays. In addition, both iron deprivation and p21 antisense blocked CD34(+) cell differentiation. These observations were not explained by induction of widespread apoptosis under conditions of iron deprivation. We concluded that both iron and functional p21(WAF1/CIP1) are required for in vitro differentiation of human haematopoietic precursors along multiple cell lineages.


Asunto(s)
Línea Celular Tumoral/fisiología , Inhibidor p21 de las Quinasas Dependientes de la Ciclina/genética , Hematopoyesis/genética , Hierro/metabolismo , Antígenos CD34/genética , Elementos sin Sentido (Genética)/genética , Apoptosis/fisiología , Butiratos/farmacología , Carcinógenos/farmacología , Recuento de Células , Diferenciación Celular/efectos de los fármacos , Línea Celular Tumoral/efectos de los fármacos , Supervivencia Celular/fisiología , Células Cultivadas , Deferoxamina/farmacología , Dimetilsulfóxido/farmacología , Regulación Leucémica de la Expresión Génica/genética , Factor Estimulante de Colonias de Granulocitos y Macrófagos/farmacología , Células HL-60/efectos de los fármacos , Células HL-60/fisiología , Hematopoyesis/efectos de los fármacos , Humanos , Células K562/efectos de los fármacos , Células K562/fisiología , Sideróforos/farmacología , Acetato de Tetradecanoilforbol/farmacología , Transcripción Genética/genética
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